Background: Because of its wonderful taste, as well as
numerous nutritional and medicinal qualities valued especially
in the 20th century apple fruit is preferred worldwide.
Objective: The current work presents the antioxidant
specifications of several varieties of apples distributed in
Republic of Bulgaria and also methodology/equipment and
method for the preparation of the apples for the experiment/
used to determine the antioxidant characteristics.
Methods: We use the following methods:
spectrophotometric method; systematic approach and a critical
analysis of the accessible Research Periodicals
Results: We obtain nectar from apples by their
immediate squeezing prior the experiment using a juicer. (1)
Mix the three tubes with electromagnetic stirrer VORTEX 2
GENIE for 10 seconds. (2) Place the tubes in a water bath for
10-15 minutes at 380C. (3) Put the tubes in cold water for 5
seconds to stop the reaction. (4.) Identification of the activity of
the samples with a UV-VIS spectrophotometer; ThermoScientific;
Used length λ = 560 nm.
CONCLUSION:
1. Choose fully ripe fruits to generate the highest
amount of antioxidants.
2. The studied varieties of apples can be arranged
according to their antioxidant activity as follows: Grey smith,
Aidere, Golden Delicious, Jonah Gold, Golden Delicious, Red
Delicious, Chadeo, Mutsu, and Florina
Extraction of Colorant From Dragon Fruit Peel and It's Application in a baker...Salemir Hossain
This is a project or thesis proposal presentation for B.Sc Engineering level on Extraction of Colorant From Dragon Fruit Peel and It's Application in a bakery Product. If any help is needed then contact on salemirhossainjust@gmail.com
Fruit as Medicine: blueberry, Genus vacciniumKevin KF Ng
Blueberry is native to North America. Commercial cultivation began in 1911. Its health benefits are related to its antioxidant property which ranks one of the highest among fruits.
Fruit as Medicine: Pomegranate, Puna granatumKevin KF Ng
Pomegranate is known for its medicinal properties for more than 5000 years. Modern science has uncovered its antioxidant, anti-inflammatory,anti-cancer,anti-hyperlipidemic, anti-diabetic, anti-hypertensive and antimicrobial properties. Current research is now focused on inflammatory disease and cancer.
Extraction of Colorant From Dragon Fruit Peel and It's Application in a baker...Salemir Hossain
This is a project or thesis proposal presentation for B.Sc Engineering level on Extraction of Colorant From Dragon Fruit Peel and It's Application in a bakery Product. If any help is needed then contact on salemirhossainjust@gmail.com
Fruit as Medicine: blueberry, Genus vacciniumKevin KF Ng
Blueberry is native to North America. Commercial cultivation began in 1911. Its health benefits are related to its antioxidant property which ranks one of the highest among fruits.
Fruit as Medicine: Pomegranate, Puna granatumKevin KF Ng
Pomegranate is known for its medicinal properties for more than 5000 years. Modern science has uncovered its antioxidant, anti-inflammatory,anti-cancer,anti-hyperlipidemic, anti-diabetic, anti-hypertensive and antimicrobial properties. Current research is now focused on inflammatory disease and cancer.
A Prospective Study of Biochemical Assay in Mice Fed Refined Sugar Diet and U...iosrjce
IOSR Journal of Dental and Medical Sciences is one of the speciality Journal in Dental Science and Medical Science published by International Organization of Scientific Research (IOSR). The Journal publishes papers of the highest scientific merit and widest possible scope work in all areas related to medical and dental science. The Journal welcome review articles, leading medical and clinical research articles, technical notes, case reports and others.
New Schiff base ligand (E)-6-(2-(4-
(dimethylamino)benzylideneamino)-2-phenylacetamido)-3,3-
dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic
acid = (HL) Figure(1) was prepared via condensation of
Ampicillin and 4(dimethylamino)benzaldehyde in methanol
.Polydentate mixed ligand complexes were obtained from 1:1:2
molar ratio reactions with metal ions and HL, 2NA on reaction
with MCl2 .nH2O salt yields complexes corresponding to the
formulas [M(L)(NA)2Cl] ,where M =
Fe(II),Co(II),Ni(II),Cu(II),and Zn(II) and NA=nicotinamide.
The 1H-NMR, FT-IR, UV-Vis and elemental analysis
were used for the characterization of the ligand. The complexes
were structurally studied through AAS, FT-IR, UV-Vis,
chloride contents, conductance, and magnetic susceptibility
measurements. All complexes are non-electrolytes in DMSO
solution. Octahedral geometries have been suggested for each
of the complexes. The Schiff base ligands function as
tridentates and the deprotonated enolic form is preferred for
coordination. In order to evaluate the effect of the bactericidal
activity, these synthesized complexes, in comparison to the un
complexed Schiff base has been screened against bacterial
species, Staphy
Attitudes toward suicide may influence the
treatment content and outcomes. Hence, this study aimed to
investigate how public attitudes toward suicide were influenced
by (1) their degree of idealism; and (2) their degree of
relativism. A questionnaire survey with Suicide Perception
Scale and Ethic Position Questionnaire was carried out on 50
male and 50 female participants (aged 21 and above) from
Klang Valley, Malaysia to obtain answer. The findings
supported both hypotheses, indicated that (1) higher idealism is
associated with lower level of acceptance toward suicide; and
(2) higher relativism is associated with higher level of
acceptance toward suicide. In sum, variations in public’s
attitude toward suicide were related to individual differences in
personal ethical ideologies and moral philosophies.
Caterer | Asian Restaurants - Chef Patricks KitchenLance Libot
Chef Patrick’s Kitchen is a local Chinese restaurant that focuses in delighting the palates of each and every guest with various fresh and delicious dishes all at affordable prices.
The main aim of energy efficient routing is to
minimize the energy required to transmit or receive packets also
called as active communication energy. Inactive energy is the
energy which not only tries to reduce the energy consumed
when a mobile node stays idle but also listens to the wireless
medium for any possible communication requests from other
nodes. To conserve energy, many energy efficient routing
protocols have been proposed. Networks of small, inexpensive,
disposable, smart sensors are emerging as a new technology with
tremendous potential. Wireless sensor networks can be randomly
deployed inside or close to phenomenon to be monitored. The
advantage of these networks is the fact that they are selfconfiguring,
which means that a sensor network can be deployed
randomly on a battlefield, in a disaster area or in an inaccessible
area without the need for human intervention. The energy
supplies of nodes are not replenished or replaced and therefore
nodes only participate in the network for as long as they have
energy. This fact necessitates energy efficiency in the design of
every aspect of such nodes. Energy consumption in sensor nodes
occurs mainly due to computational processing and, to a greater
extent, communication. The routing protocol employed by these
sensor nodes can minimize the number of transmissions that
nodes make as well as the computational complexity of routing
path selection. It is therefore of critical importance that the
routing protocol be designed with energy efficiency in mind.
Batch adsorption experiments were carried out for
the adsorption of cationic dye from aqueous solution onto
composite activated carbon. The composite activated carbon was
prepared from brewer’s spent grain and sea bean shell at a ratio
of 1:1. The equilibrium studies were done at different
concentrations and temperatures. The equilibrium data were
fitted to Langmuir, Freundlich, Dubinin-Radushkevich, and
Temkin isotherm models. The results showed that both Lagmuir
and Freundlich isotherm model fitted the data reasonably well
but Freundlich isotherm fitted better in the temperature range
studied. This confirmed that the adsorption is heterogeneous,
non-specific and non-uniform in nature. Kinetic studies were also
undertaken in terms of first order, second order, pseudo first
order, pseudo second order, Elovich, Boyd, and intra-particle
diffusion models. The results indicated that the data followed
pseudo second order model with surface adsorption and intraparticle
diffusion concurrently operating during the adsorbateadsorbent
interaction. The values of the thermodynamic
parameters computed from Van’t Hoff plot confirmed the
process to be endothermic and spontaneous in nature.
Data mining , knowledge discovery is the process
of analyzing data from different perspectives and summarizing it
into useful information - information that can be used to increase
revenue, cuts costs, or both. Data mining software is one of a
number of analytical tools for analyzing data. It allows users to
analyze data from many different dimensions or angles, categorize
it, and summarize the relationships identified. Technically, data
mining is the process of finding correlations or patterns among
dozens of fields in large relational databases. The goal of
clustering is to determine the intrinsic grouping in a set of
unlabeled data. But how to decide what constitutes a good
clustering? It can be shown that there is no absolute “best”
criterion which would be independent of the final aim of the
clustering. Consequently, it is the user which must supply this
criterion, in such a way that the result of the clustering will suit
their needs.
For instance, we could be interested in finding
representatives for homogeneous groups (data reduction), in
finding “natural clusters” and describe their unknown properties
(“natural” data types), in finding useful and suitable groupings
(“useful” data classes) or in finding unusual data objects (outlier
detection).Of late, clustering techniques have been applied in the
areas which involve browsing the gathered data or in categorizing
the outcome provided by the search engines for the reply to the
query raised by the users. In this paper, we are providing a
comprehensive survey over the document clustering.
A Prospective Study of Biochemical Assay in Mice Fed Refined Sugar Diet and U...iosrjce
IOSR Journal of Dental and Medical Sciences is one of the speciality Journal in Dental Science and Medical Science published by International Organization of Scientific Research (IOSR). The Journal publishes papers of the highest scientific merit and widest possible scope work in all areas related to medical and dental science. The Journal welcome review articles, leading medical and clinical research articles, technical notes, case reports and others.
New Schiff base ligand (E)-6-(2-(4-
(dimethylamino)benzylideneamino)-2-phenylacetamido)-3,3-
dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic
acid = (HL) Figure(1) was prepared via condensation of
Ampicillin and 4(dimethylamino)benzaldehyde in methanol
.Polydentate mixed ligand complexes were obtained from 1:1:2
molar ratio reactions with metal ions and HL, 2NA on reaction
with MCl2 .nH2O salt yields complexes corresponding to the
formulas [M(L)(NA)2Cl] ,where M =
Fe(II),Co(II),Ni(II),Cu(II),and Zn(II) and NA=nicotinamide.
The 1H-NMR, FT-IR, UV-Vis and elemental analysis
were used for the characterization of the ligand. The complexes
were structurally studied through AAS, FT-IR, UV-Vis,
chloride contents, conductance, and magnetic susceptibility
measurements. All complexes are non-electrolytes in DMSO
solution. Octahedral geometries have been suggested for each
of the complexes. The Schiff base ligands function as
tridentates and the deprotonated enolic form is preferred for
coordination. In order to evaluate the effect of the bactericidal
activity, these synthesized complexes, in comparison to the un
complexed Schiff base has been screened against bacterial
species, Staphy
Attitudes toward suicide may influence the
treatment content and outcomes. Hence, this study aimed to
investigate how public attitudes toward suicide were influenced
by (1) their degree of idealism; and (2) their degree of
relativism. A questionnaire survey with Suicide Perception
Scale and Ethic Position Questionnaire was carried out on 50
male and 50 female participants (aged 21 and above) from
Klang Valley, Malaysia to obtain answer. The findings
supported both hypotheses, indicated that (1) higher idealism is
associated with lower level of acceptance toward suicide; and
(2) higher relativism is associated with higher level of
acceptance toward suicide. In sum, variations in public’s
attitude toward suicide were related to individual differences in
personal ethical ideologies and moral philosophies.
Caterer | Asian Restaurants - Chef Patricks KitchenLance Libot
Chef Patrick’s Kitchen is a local Chinese restaurant that focuses in delighting the palates of each and every guest with various fresh and delicious dishes all at affordable prices.
The main aim of energy efficient routing is to
minimize the energy required to transmit or receive packets also
called as active communication energy. Inactive energy is the
energy which not only tries to reduce the energy consumed
when a mobile node stays idle but also listens to the wireless
medium for any possible communication requests from other
nodes. To conserve energy, many energy efficient routing
protocols have been proposed. Networks of small, inexpensive,
disposable, smart sensors are emerging as a new technology with
tremendous potential. Wireless sensor networks can be randomly
deployed inside or close to phenomenon to be monitored. The
advantage of these networks is the fact that they are selfconfiguring,
which means that a sensor network can be deployed
randomly on a battlefield, in a disaster area or in an inaccessible
area without the need for human intervention. The energy
supplies of nodes are not replenished or replaced and therefore
nodes only participate in the network for as long as they have
energy. This fact necessitates energy efficiency in the design of
every aspect of such nodes. Energy consumption in sensor nodes
occurs mainly due to computational processing and, to a greater
extent, communication. The routing protocol employed by these
sensor nodes can minimize the number of transmissions that
nodes make as well as the computational complexity of routing
path selection. It is therefore of critical importance that the
routing protocol be designed with energy efficiency in mind.
Batch adsorption experiments were carried out for
the adsorption of cationic dye from aqueous solution onto
composite activated carbon. The composite activated carbon was
prepared from brewer’s spent grain and sea bean shell at a ratio
of 1:1. The equilibrium studies were done at different
concentrations and temperatures. The equilibrium data were
fitted to Langmuir, Freundlich, Dubinin-Radushkevich, and
Temkin isotherm models. The results showed that both Lagmuir
and Freundlich isotherm model fitted the data reasonably well
but Freundlich isotherm fitted better in the temperature range
studied. This confirmed that the adsorption is heterogeneous,
non-specific and non-uniform in nature. Kinetic studies were also
undertaken in terms of first order, second order, pseudo first
order, pseudo second order, Elovich, Boyd, and intra-particle
diffusion models. The results indicated that the data followed
pseudo second order model with surface adsorption and intraparticle
diffusion concurrently operating during the adsorbateadsorbent
interaction. The values of the thermodynamic
parameters computed from Van’t Hoff plot confirmed the
process to be endothermic and spontaneous in nature.
Data mining , knowledge discovery is the process
of analyzing data from different perspectives and summarizing it
into useful information - information that can be used to increase
revenue, cuts costs, or both. Data mining software is one of a
number of analytical tools for analyzing data. It allows users to
analyze data from many different dimensions or angles, categorize
it, and summarize the relationships identified. Technically, data
mining is the process of finding correlations or patterns among
dozens of fields in large relational databases. The goal of
clustering is to determine the intrinsic grouping in a set of
unlabeled data. But how to decide what constitutes a good
clustering? It can be shown that there is no absolute “best”
criterion which would be independent of the final aim of the
clustering. Consequently, it is the user which must supply this
criterion, in such a way that the result of the clustering will suit
their needs.
For instance, we could be interested in finding
representatives for homogeneous groups (data reduction), in
finding “natural clusters” and describe their unknown properties
(“natural” data types), in finding useful and suitable groupings
(“useful” data classes) or in finding unusual data objects (outlier
detection).Of late, clustering techniques have been applied in the
areas which involve browsing the gathered data or in categorizing
the outcome provided by the search engines for the reply to the
query raised by the users. In this paper, we are providing a
comprehensive survey over the document clustering.
Мы организовываем 5 недель активностей, которые взорвут вашу группу! Что сделать, чтобы полюбиться участникам? Как создать элиту группы? Как заполучить доверие и лояльность? Об этом в нашей презентации!
World Wide Web is large sized repository of
interlinked hypertext documents accessed via the Internet. Web
may contain text, images, video, and other multimedia data. The
user navigates through this using hyperlink. Search Engine gives
millions of results and applies Web mining techniques to order the
results. The sorted order of search results is obtained by applying
some special algorithms called—Page ranking algorithms. The
algorithm measures the importance of the pages by analyzing the
number of inlinked and outlinked pages. Our proposed system is
built on an idea that to rank the relevant pages higher in the
retrieved document set, an analysis of both page‘s text substance
and links information is required. The proposed approach is
based on the assumption that the effective weight of a term in a
page is computed by adding the weight of a term in the current
page and additional weight of the term in the linked pages. In
this chapter, we first study the nature of web pages, the various
link analysis ranking algorithms and their limitations and then
show the comparative analysis of the ranking scores obtained
through these approaches with our new suggested ranking
approach.
Nutritional Profile and Physicochemical Properties of Peach Varieties in Ethi...AJSERJournal
Nutritional and functional characteristics of fruits are related to their quality and are influenced by
genotype and ripening stage, and by environmental conditions and orchard management practices. The purpose of this
research was to test nutritional profile and selected physicochemical properties of different improved and adopted
peach varieties in Ethiopia and comparative study among varieties and between study varieties and standard
reference. The result got show that improved peach varieties greater amount of ash (4.3-5.51%), protein (4.34-6.05%),
fat (0.097-1.386%) and fiber (2.87-4.611%) than standard reference (0.263, 1.423, 0.427, and 1.20%, in the
aforementioned order) but lower in carbohydrate (84.411-89.90%) than standard of (96.678%). The mineral content
higher in K (0.48-1.182%), Ca (0.162-0.565 %), Mg (0.037-0.066%), Fe (16.33-159.2%) and Zn (2.43-8.84%) than
standard reference (0.942, 0.051, 0.071, 16.32, 3.213%), respectively except Na, Mg, Cu and Sulfur while the fruit
quality was revealed low moisture content which less water and medium vitamin C (3.99-5.55%) and TSS (8.36-
14.31%). We observed variation in proximate composition and mineral content among sixteen peach varieties.
Screening and evaluation of medicinal properties of grape fruit varieties in ...EditorIJAERD
Theni districts could be considered one of the highest grapes producing area also export best quality of
Paneer dhrakshai Indian table grapes in Tamil Nadu. The research work focused to identify the nutritive compounds in
the grapes (Vitis vinifera) species which are a wonderful fruit having pulp content, vibrant color and exert an immense
health benefits. Grape fruits were found to be rich source of Anthocyanin content and vitamin C also macromolecules
contents such as carbohydrate contents of 29mg, Protein 18.7mg and fat content 3.7mg /100 g of f. wt. Micronutrients
were estimated by AAS which showed that calcium content was 3.1mg/g.f.wt, iron content 2.7 mg/g.f.wt, potassium 2.6
mg/g.f.wt found to be more than other minerals in fresh extracts of grape fruits. The comparative results of revealed
considerable amount of phytoconstituents in grape fruits in which anthocyanins alone has been taken to evaluate
therapeutic effects on breast cancer cell MCF-7 cell viability test was carried out to determine cell proliferation. The
results revealed presence of anthocyanins in high quantity in the process of purification through various biochemical
methods that could ultimately be used for preventing cancerous cell proliferation which reduces the development of
cancer diseases. On comparison Grapes (Vitis vinifera) may serve as functional food with vital pharmaceutical and
biological value.
‘Cider wine production using different yeast strains & comparing their efficiency, testing their antimicrobial activity towards E.coli’ fortification with honey for increasing vit b content and helping initial clarification. project author :Ritwik Bhatatcharya, MSc Food technology, BSc (hons)Microbiology. Done as part of Final smseter research project from Punjabi university , Patiala.wokring in QC(wine).Prodcution(FMCG), R&D. check website www.indobrews.com for more information,
Email us at brewindo@gmail.com
cider wine preparation using different yeast culturesguest6de666
cider wine preparation using honey and different yeast cultures (Saccharomyces cerevisiae and Saccharomyces uvarum), testing their efficiency based on alcohol production, in single and mixed cultures, fortification by honey to increase vit b content as well help in initial calrification, and testing antimicrobial character of cider types on E.coli
COMPARATIVE ANALYSIS OF ASCORBIC ACID CONCENTRATION IN TWO VARIETIES OF CITRU...mujahid hussain
COMPARATIVE ANALYSIS OF ASCORBIC ACID CONCENTRATION IN TWO VARIETIES OF CITRUS (CITRUS SINENSIS, CITRUS LIMETTA) COLLECTED FROM DIFFERENT TEHSILS OF DISTRICT SARGODHA
In the present study we monitor the eff ect of copper-contaminated culture media on development and active compound biosynthesis of two Rhodotorula mucilaginosa strains, assigned as R1 and R2. The media was prepared in aqueous extracts of Asclepias syriaca stems and diff erent copper ions concentrations were added. The data reveals that the presence of 10 mg/ L Cu2+ for the Rhodotorula mucilaginosa strain-1 (R1) and 50 mg/ L Cu2+ for the Rhodotorula mucilaginosa strain-2 (R2) strain fermentation increases the biomass yield (24.7 g and 14.8 g wet biomass/ L culture medium, respectively) and biosynthesis of carotenoid pigments (approximately 1.4 μg/ g dry biomass) compared with references. The chelating properties of polyphenol compounds from aqueous extracts of Asclepias syriaca
stems was confi rmed by detection of 17.9 mg/ L Cu2+ in extracts with 100 mg/ L Cu2+ added, after 24 hours at 4°
C. In the residual culture media, we have determines that the Cu2+ concentrations decreased at 14.0 mg/L for Rhodotorula mucilaginosa strain-1 (R1) and 13.8 mg/
L for Rhodotorula mucilaginosa strain-2 (R2) strain fermentation, respectively, which means that the copper was trapped by the yeasts cells. Likewise, the polyphenols are digested and used as a carbon source. These results bring a significant contribution to the possibility of yeast fermentations in a low cost-vegetal polyphenol and copper ions system.
Production of alcohol by yeast isolated from apple, orange and bananaPremier Publishers
The purpose of this study was to isolate wild yeast strains present in different fruits (apple, orange and banana) and to determine the yeast growth and the amount of alcohol production at various glucose concentrations. Three fruits namely apple, banana and orange were selected as natural sources for yeast isolation. Medium used for isolation of yeast from fruits was consisting of 50 glucose, 3 malt extract, 3 yeast extract, 5 peptone and 15g/L agar. For fermentation MGYP medium used with different glucose concentrations of 5, 10, 30, 50 and 70g/L. Inocula were prepared by loop transfers from stock slants to 50 mL of the 20 percent medium in 500-mL Erlenmeyer flasks. The results showed that with higher concentration of glucose (30g/L) higher amount of alcohol (0.22g/100mL) was produced. Similarly, the yeast isolated from apple showed maximum yeast biomass (0.38g/100mL) at 70g/L glucose concentration and minimum on 50g/L (0.03g/100mL) glucose concentration. In conclusion, the wild yeast produce higher ethanol amount in case of banana fruits.
Changes in functional compounds, antioxidant capacity, sensory profile of paste, in the processing of cocoa beans, from three geographic areas
Aide Quispe1, Pedro Camasca 2, Pedro P. Peláez 1*
1 Universidad Nacional Agraria de la Selva, Carretera Central 1,21, Tingo María, Perú.
2 Cooperativa Agraria Cafetalera Divisoria Ltda, Caserío Mapresa km. 4.40 C.F.B. Santa Martha, Tingo María, Perú.
* Corresponding author:
Pedro Pablo Peláez Sánchez
E-mail address: pedro.pelaez@unas.edu.pe
Tel: +51 962686829
Similar to ANTIOXIDANT CHARACTERISTICS OF DIFFERENT VARIETIES OF APPLES DISTRIBUTED IN BULGARIA (20)
Recent joint surgery studies reveal increased
revisions and resurfacing of the metal on metal hip joints. Metal
on metal hip implants were developed more than thirty years ago
and their application has been refined because of availability of
advanced manufacturing techniques and partly by advancements
in material science and engineering. Development of composite
materials may provide greater durability to metal-on-metal hip
implants .This review article is a study of the latest literature of
metal-on-metal hip implants and its various modeling techniques.
Numbers of methods are used for convergence and numerical
solution to investigate the performance of metal-on-metal hip
implant for accurate stable solution. This paper presents analysis
done by various researchers on metal-on-metal hip implants for
wear, lubrication, fatigue, bio-tribo-corrosion, design, toxicity
and resurfacing. After in vivo and in vitro studies, it is found that
all these methods have limitations. There is a need of more
insight for lubrication analysis, geometry of bearings, materials
and input parameters. The information provided in this work is
intended as an aid in the assessment of metal-on-metal hip joints.
Background Hospital contributes significantly tangible and intangible resources on a concurred plan by the scheduling of surgery on the OT list. Postponement decreases efficiency by declining throughput leads to wastage of resources hence burden to the nation. Patients and their family face economic and emotional implication due to the postponement. Postponement rate being a quality indicator controls check mechanism could be developed from the results. Postponement of elective scheduled operations results in inefficient use of the operating room (OR) time on the day of surgery. Inconvenience to patients and families are also caused by postponements. Moreover, the day of surgery (DOS) postponement creates logistic and financial burden associated with extended hospital stay and repetitions of pre-operative preparations to an extent of repetition of investigations in some cases causing escalated costs, wastage of time and reduced income. Methodology A cross-sectional study was done in the operation theaters of a tertiary care hospital in which total ten operation theaters of General Surgery Data of scheduled, performed and postponed surgeries was collected from all the operation theater with effect from March 1st to September 30th, 2018. A questionnaire was developed to find out the reasons for the postponement for all hospital’s stakeholders (surgeons, Anesthetist, Nursing Officer) and they were further evaluated time series analysis of scheduling of Operation Theater for moving average technique. Results Total 958 surgeries were scheduled and 772 surgeries performed were and 186 surgeries were postponed with a postponement rate of 19.42% in the cardiac surgery department during the study period. Month-wise postponement Rate exponential smoothing of time series data shows the dynamic of operating suits. To test throughput Postponement rate was plotted the postponed surgeries and on regression analysis is in a perfect linear relationship.
Introduction: Postponement of elective scheduled operations results in inefficient use of operating room (OR) time on the day of surgery. Inconvenience to patients and families also caused by postponements. Moreover, day of surgery (DOS) postponement creates logistic and financial burden associated with extended hospital stay and repetitions of pre-operative preparations to an extend of repetition of investigations in some cases causing escalated costs, wastage of time and reduced income. Methodology: A cross sectional study was done in the operation theaters of a tertiary care hospital in which total ten operation theaters of General Surgery Data of scheduled, performed and postponed surgeries was collected from all the operation theater with effect from march 1st to September 30th 2018. A questionnaire was developed to find out the reasons for the postponement for all hospital’s stakeholders (Surgeons, Anesthetist, Nursing officer) and they were further evaluated Time series analysis of scheduling of Operation Theater for Moving average Technique. Results: total 2,466 surgeries were scheduled and 1,980 surgeries were performed and 486 surgeries were postponed in the general surgery department during the study period. Month wise postponement forecast was in accordance with the performed surgeries and on regression analysis postponed surgeries were in perfect linear relationship with the postponement Rate.
In the present paper the experimental study of
Nanotechnology involves high cost for Lab set-up and the
experimentation processes were also slow. Attempt has also
been made to discuss the contributions towards the societal
change in the present convergence of Nano-systems and
information technologies. one cannot rely on experimental
nanotechnology alone. As such, the Computer- simulations and
modeling are one of the foundations of computational
nanotechnology. The computer modeling and simulations
were also referred as computational experimentations. The
accuracy of such Computational nano-technology based
experiment generally depends on the accuracy of the following
things: Intermolecular interaction, Numerical models and
Simulation schemes used. The essence of nanotechnology is
therefore size and control because of the diversity of
applications the plural term nanotechnology is preferred by
some nevertheless they all share the common feature of control
at the nanometer scale the latter focusing on the observation
and study of phenomena at the nanometer scale. In this paper,
a brief study of Computer-Simulation techniques as well as
some Experimental result
Solar cell absorber Kesterite- type Cu2ZnSnS4 (CZTS) thin films have been prepared by Chemical Bath Deposition (CBD). UV–vis absorption spectra measurement indicated that the band gap of as-synthesized CZTS was about1.68 eV, which was near the optimum value for photovoltaic solar conversion in a single-band-gap device. The polycrystalline CZTS thin films with kieserite crystal structure have been obtained by XRD. The average of crystalline size of CZTS is 27 nm
Multilevel inverters play a crucial part in the
areas of high and medium voltage applications. Among the three
main multilevel inverters used, the capacitor clamped multilevel
inverter(CCMLI) has advantage with respect to voltage
redundancies. This work proposes a switching pattern to improve
the performance of chosen H-bridge type CCMLI over
conventional CCMLI. The PWM technique used in this work is
Phase Opposition Disposition PWM(PODPWM). The
performance of proposed H-bridge type CCMLI is verified
through MATLAB-Simulink based simulation. It has been
observed that the THD is low in chosen CCMLI compared to
conventional CCMLI.
- In this paper, we introduce a practical mechanism of
compressing a binary phase code modulation (BPCM) signal
according to Barker code with 13 chips in presence of additive
white Gaussian noise (AWGN) by using a digital matched filter
(DMF) corresponding to time domain convolution algorithm of
input and reference signals using Cyclone II EP2C70F896C6
FPGA from ALTERA placed on education and development
board DE2-70 with the following parameters: frequency of
BPCM signal fIF=2 MHz, sampling frequency
f MHz SAM 50
,pulse period
T 200s
, pulse width
S 13sc
, chip width
CH 1sc
, compressing factor
KCOM 13
, SNRinp=1/1, 1/2, 1/3, 1/4, 1/5 and processing
gain factor SNRout/SNRinp=11.14 dB.
The results of filter operation are evaluated using a digital
oscilloscope GDS-1052U to display the input and output signals
for different SNRinp.
Flooding is one of the most devastating natural
disasters in Nigeria. The impact of flooding on human activities
cannot be overemphasized. It can threaten human lives, their
property, environment and the economy. Different techniques
exist to manage and analyze the impact of flooding. Some of these
techniques have not been effective in management of flood
disaster. Remote sensing technique presents itself as an effective
and efficient means of managing flood disaster. In this study,
SPOT-10 image was used to perform land cover/ land use
classification of the study area. Advanced Space borne Thermal
Emission and Reflection Radiometer (ASTER) image of 2010 was
used to generate the Digital Elevation Model (DEM). The image
focal statistics were generated using the Spatial Analyst/
Neighborhood/Focal Statistics Tool in ArcMap. The contour map
was produced using the Spatial Analyst/ Surface/ Contour Tools.
The DEM generated from the focal statistics was reclassified into
different risk levels based on variation of elevation values. The
depression in the DEM was filled and used to create the flow
direction map. The flow accumulation map was produced using
the flow direction data as input image. The stream network and
watershed were equally generated and the stream vectorized. The
reclassified DEM, stream network and vectorized land cover
classes were integrated and used to analyze the impact of flood on
the classes. The result shows that 27.86% of the area studied will
be affected at very high risk flood level, 35.63% at high risk,
17.90% at moderate risk, 10.72% at low risk, and 7.89% at no
risk flood level. Built up area class will be mostly affected at very
high risk flood level while farmland will be affected at high risk
flood level. Oshoro, Imhekpeme, and Weppa communities will be
affected at very high risk flood inundation while Ivighe, Uneme,
Igoide and Iviari communities will be at risk at high risk flood
inundation level. It is recommended among others that buildings
that fall within the “Very High Risk” area should be identified
and occupants possibly relocated to other areas such as the “No
Risk” area.
Without water, humans cannot live. Since time began,
we have lived by the water and vast tracts of waterless land have
been abandoned as it is too difficult to inhabit. At any given
moment, the earth’s atmosphere contains 4,000 cubic miles of
water, which is just 0.000012% of the 344 million cubic miles of
water on earth. Nature maintains this ratio via evaporation and
condensation, irrespective of the activities of man.
There is a certain need for an alternative to solve the water
scarcity. Obtaining water from the atmosphere is nothing new -
since the beginning of time, nature’s continuous hydrologic cycle
of evaporation and condensation in the form of rain or snow has
been the sole source and means of regenerating wholesome water
for all forms of life on earth.
An effective method to generate water is by the separation of
moisture present in air by condensation. In this study, the water
present in air is condensed on the surface of a container and then
collected in an external jacket provided on the container.
Insulations are provided to optimize the inner temperature of the
container.
The method is although uncommon but has certain advantages
which make it a success. The process is economical and does not
require a lot of utilities. It also helps in further reducing the
carbon footprint.
In every moment of functioning the Li-Ion
battery must provide the power required by the user, to have a
long operating life and to and to provide high reliability in
operation. The methods for analysis and testing batteries are
ensuring that all these conditions imposed to the batteries are
met by being tested depending on their intended use.
The success rate of real estate project is
decreasing as there is large scale of project and participation of
entities. It is necessary to study the risk factors involved in the
project. This paper focused on types of risks involved in the
project, risk factors, risk management tools & techniques.
Identification of risk of the project in terms of the total cost of the
project has been divided under Technical, Financial, Sociopolitical
and Statutory cost centers. Large real estate projects
have to tackle the following issues: land acquisition, skilledlabour
shortage, non-availability of skilled project managers, and
mechanization of the construction process to cater to the growing
demands. Non- availability of supporting infrastructure, political
issues like instability of the government leading to regulatory
issues, social issues, marketing forms an important part in these
projects as this is a onetime investment and the purchase cycle is
long , long development period makes the same project be at
different points in the real estate value cycle.
- In the present scenario carbon emission and sand
mining are major concern due to its hazardous effect to
environment and making serious imbalance to the ecosystem.
Various studies have been conducted to reduce severe effect on
environment, using byproducts like copper slag as partial
replacement of fine aggregate. Different researchers have also
revealed numerous uses of copper slag as a replacing agent in
determining the strength of concrete. A comprehensive review of
studies has been presented in this paper for scope of replacement
of fine aggregate from copper slag in concrete
- Security is a concept similar to being cautious
or alert against any danger. Network security is the condition of
being protected against any danger or loss. Thus safety plays a
important role in bank transactions where disclosure of any data
results in big loss. We can define networking as the combination
of two or more computers for the purpose of resource sharing.
Resources here include files, database, emails etc. It is the
protection of these resources from unauthorized users that
brought the development of network security. It is a measure
incorporated to protect data during their transmission and also
to ensure the transmitted is protected and authentic.
Security of online bank transactions here has been
improved by increasing the number of bits while establishing the
SSL connection as well as in RSA asymmetric key encryption
along with SHA1 used for digital signature to authenticate the
user
Background: Septoplasty is a common surgical
procedure performed by otolaryngologists for the correction of
deviated nasal septum. This surgery may be associated with
numerous complications. To minimize these complications,
otolaryngologists frequently pack both nasal cavities with
different types of nasal packing. Despite all its advantages,
nasal packing is also associated with some disadvantages. To
avoid these issues, many surgeons use suturing techniques to
obviate the need for packing after surgery.
Objective: To determine the efficacy and safety of trans-septal
suture technique in preventing complications and decreasing
morbidity after septoplasty in comparison with nasal packing.
Patients and methods: Prospective comparative study. This
study was conducted in the department of Otolaryngology -
Head and Neck Surgery, Rizgary Teaching Hospital - Erbil,
from the 6th of May 2014 to the 30th of November 2014.
A total of 60 patients aged 18-45 years, undergoing septoplasty,
were included in the study. Before surgery, patients were
randomly divided into two equal groups. Group (A) with transseptal
suture technique was compared with group (B) in which
nasal packing with Merocel was done. Postoperative morbidity
in terms of pain, bleeding, postnasal drip, sleep disturbance,
dysphagia, headache and epiphora along with postoperative
complications including septal hematoma, septal perforation,
crustation and synechiae formation were assessed over a follow
up period of four weeks.
Results: Out of 60 patients, 37 patients were males (61.7%)
and 23 patients were females (38.3%). Patients with nasal
packing had significantly more postoperative pain (P<0.05)><0.05). There was no significant difference between
the two groups with respect to nasal bleeding, septal
hematoma, septal perforation, crustation and synechiae
formation.
Conclusion: Septoplasty can be safely performed using transseptal
suturing technique without nasal packing.
The basic reason behind the need to
monitor water quality is to verify whether the examined
water quality is suitable for intended usage or not. This
study is conducted on Al -Shamiya al- sharqi drain in
Diwaniya city in Iraq to make valid assessment for the
level of parameters measured and to realize their effects
on irrigation. In order to assess the drainage water
quality for irrigation purposes with a high accuracy, the
Irrigation Water Quality Index (IWQI) will be examined
and upgraded (integrated with GIS) to make a
classification for drainage water. For this purpose, ten
samples of drainage water were taken from different ten
location of the stuay area. The collected samples were
analyzed chemically for different elements which affect
water quality for irrigation.These elements are :
Calcium(Ca+2), Sodium(Na+
), Magnesium(Mg+2),
Chloride( ), Potassium(K+
), Bicarbonate(HCO3),
Nitrate(NO3), Sulfate( , Phosphate( , Electrical
Conductivity(EC), Total Dissolved Solids (TDS), Total
Suspended Solids (TSS) and pH-values (PH). Sodium
Adsorption Ratio (SAR) and Sodium Content (Na%)
have been also calculated. Results suggest that, the use of
GIS and Water Quality Index (WQI) methods could
provide an extremely interesting as well as efficient tool
to water resource management. The results analysis of
(IWQI) maps confirms that: 52% of the drainage water
in study area falls within the "Low restriction" (LR) and
47%of study area has water with (Moderate
restriction)(MR),While 1% of drainage water in the
study area classified as (Sever restriction) (SR). So, the
drainage water should be used with the soil having high
permeability with some constraints imposed on types of
plant for specified tolerance of salts
The cable-hoisting method and rail cable-lifting
method are widely used in the construction of suspension bridge.
This paper takes a suspension bridge in Hunan as an example,
and expounds the two construction methods, and analyzes their
respective merits and disadvantages.
Baylis-Hillman reaction has been achieved on
different organic motifs but with completion times of three to
six days. Micellar medium of CTAB in water along with the
organic base DABCO has been used to effect the BaylisHillman
reaction on a steroidal nucleus of Withaferin-A for the
first time with different aromatic aldehydes within a day to
synthesize a library of BH adducts (W1a –W14a) and (W1bW14b)
as a mixture of two isomers and W15 as a single
compound. The isomers were separated on column and the
major components were chosen for bio-evaluation. Cytotoxic
activity of the synthesized compounds was screened against a
panel of four cancer cell lines Lung A-549, Breast MCF-7,
Colon HCT-116 and Leukemia THP-1 along with 5-florouracil
and Mitomycin-C as references. All the compounds exhibited
promising activity against screened cell lines and were found to
possess enhaunced activity than parent compound. BH adducts
with aromatic systems having methoxy and nitro groups were
found to be more active.
This paper presents the details on the
experimental investigation carried out to get the desired fresh
properties of the SCC. Tests were performed on various mixtures
to obtain the required SCC. In the present research work we
have replaced 15% of cement with class F fly ash. By varying the
quantity of water and sand the mortar mix was prepared. Later
varying percentage of coarse aggregate was added to the mortar
to obtain the desired SCC.
The batteries used in electric and hybrid vehicles
consists of several cells with voltages between 3.6V battery and
4.2 V in series or parallel combinations of configurations for
obtaining the necessary available voltages in the operation of a
hybrid electric vehicle. How malfunction of a single cell affects
the behavior of the entire battery pack, BMS main function is to
protect individual cells against over-discharge, overload or
overheating. This is done by correct balancing of the cells. In
addition BMS estimates the battery charge status
This project aims at using (PD-MCPWM) Phase
disposition multi carrier pulse width modulation technique to
reduce leakage current in a transformerless cascaded multilevel
inverter for PV systems. Advantages of transformerless PV
inverter topology is as follows, simple structure, low weight and
provides higher efficiency , but however this topology provides a
path for the leakage current to flow through the parasitic
capacitance formed between the PV module and the ground.
Modulation technique reduces leakage current with an added
advantage without adding any extra components.
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ANTIOXIDANT CHARACTERISTICS OF DIFFERENT VARIETIES OF APPLES DISTRIBUTED IN BULGARIA
1. International Journal of Technical Research and Applications e-ISSN: 2320-8163,
www.ijtra.com Volume 3, Issue 2 (Mar-Apr 2015), PP. 33-36
33 | P a g e
ANTIOXIDANT CHARACTERISTICS OF
DIFFERENT VARIETIES OF APPLES
DISTRIBUTED IN BULGARIA
Papanov St.1, Ek.Petkova2, St.Pankova3, N.Traykova2, G.Hadjidekov4, V.Grudeva5
1
Medical University Plovdiv, Pharmacy Faculty
2
Medical University Plovdiv,Medical college
3
Medical University Sofia
4
University Kliment Ohridski Sofia, Medical Faculty
5
Hospital Ssheta Ekaterina, Sofia
'stoyan.papanov@abv.bg'
Abstract-
Background: Because of its wonderful taste, as well as
numerous nutritional and medicinal qualities valued especially
in the 20th century apple fruit is preferred worldwide.
Objective: The current work presents the antioxidant
specifications of several varieties of apples distributed in
Republic of Bulgaria and also methodology/equipment and
method for the preparation of the apples for the experiment/
used to determine the antioxidant characteristics.
Methods: We use the following methods:
spectrophotometric method; systematic approach and a critical
analysis of the accessible Research Periodicals
Results: We obtain nectar from apples by their
immediate squeezing prior the experiment using a juicer. (1)
Mix the three tubes with electromagnetic stirrer VORTEX 2
GENIE for 10 seconds. (2) Place the tubes in a water bath for
10-15 minutes at 380C. (3) Put the tubes in cold water for 5
seconds to stop the reaction. (4.) Identification of the activity of
the samples with a UV-VIS spectrophotometer; Thermo-
Scientific; Used length λ = 560 nm.
CONCLUSION:
1. Choose fully ripe fruits to generate the highest
amount of antioxidants.
2. The studied varieties of apples can be arranged
according to their antioxidant activity as follows: Grey smith,
Aidere, Golden Delicious, Jonah Gold, Golden Delicious, Red
Delicious, Chadeo, Mutsu, and Florina.
Keywords: apple, antioxidant activity
I. INTRODUCTION
The apple is well known fruit since ancient times. Besides
its marvelous taste, it is preferred for its numerous nutritious
and healing qualities that are most valued in the twentieth
century[5,6]. We believe that there is more to learn about its
origin and benefits
OBJECTIVE: This paper presents:
- The antioxidant properties of several varieties of
apples grown in Republic of Bulgaria
- Methodology (instrumentation and method for
preparing the apples for experiment) used to determine the
antioxidant characteristics.
Object of the survey are the following sorts of apples:
Golden Delicious, Red Delicious, Grey-Smith, Mutsu,
Florina, Chadeo, JonaGold, Aidere.
Information sources
Time of the study spans from September the 12 to
October the 30 2013.
Place of study: Medical University of Plovdiv -
Faculty of Pharmacy and College of Medicine, University of
Thrace
Methods applied:
- Spectrophotometric method[8,10]
- Systemic approach and critical analysis of the
available scientific periodicals[11]
Apparatus:
- UV-VIS spectrophotometer
- Thermo scientific
- Electromagnetic stirrer VORTEX 2 GENIE
- Centrifuge Centronix Microcentrifugo 1236V
- Juicer
II. RESULTS AND DISCUSSION
GENERAL - a brief review of the available scientific
periodicals
Apple refers to the Rosaceae family /Rosaceae/, apple
subfamily /Pomoidae/, genus Malus Mill. There are about 30
sorts of apples, but only few of them are progenitors of
modern varieties. The apple was known to prehistoric man.
Remains of apple fruits were found during excavations of
the pile dwellings that existed some 5,000 years ago. Its
homeland is considered to be an area in the northwestern
Himalayas. Cultivation of apple originated in Asia Minor,
and then its path leads to the Caucasus, Egypt and Palestine.
More than 600 BC Apples were cultivated in ancient
Greece, where it was taken to Rome and thence to Europe.
This culture is mentioned in ancient manuscripts in China,
Persia, India and Rome. Although it was known from
ancient times, great economic importance the apple gained
in twentieth century, when it was valued for its nutritional
and medicinal qualities[2,10].
The apple is among the best fruit foods for human. This
is due to its diverse chemical composition: dry matter
content - 8.0-13.0%; sugars; Organic acids - 0.11-0.49%;
minerals, vitamins, enzymes, fiber, mainly in the form of
pectin and cellulose. Vitamin C content varies from 4 to 80
mg. So besides being a nourishing food, the fruits have
valuable medicinal and dietary properties[9,10].
Apples are a great source of vegetable matter - i.e.
flavonoids. Particularly valuable is quercetin, which reduces
the risk of lung cancer by 46%. In 2001, American
Laboratory studies have shown that apples stop the growth
of prostate cancer cells and the risk of cancer of the
digestive system decreases by 30-40%. Due to the anti-
inflammatory properties of quercetin, apples improve lung
functioning and relieve asthma. The usage of only five
apples per week improves lung function by 3%. Another
2. International Journal of Technical Research and Applications e-ISSN: 2320-8163,
www.ijtra.com Volume 3, Issue 2 (Mar-Apr 2015), PP. 33-36
34 | P a g e
flavonoid, which can boast the apple, is catechin, and studies
confirm that people where catechin content is high in the
blood rarely suffer heart attack[3,4,7].
The amount of cellulose in apples is greater than in
other fruits and varies from 0.8 to 1.2%. Cellulose is
concentrated in the skin and seed of the fruit. Pectin (from
0.08 to 1.77%) makes apple fruit strong and gives them very
good gelling properties, which is why they are very
important for the processing industry.
The flavor of apples is largely dependent on the
amount and the ratio of the content of sugars, organic acids
and tannins. The aroma is due to the essential oils.
The content of sugars in apple fruit is 9.24 to
12.71%.
The fructose is dominant - 6.8%, glucose - 2.7%, and
sucrose - 2.2%.
The total acidity is 0.17% at the sweet varieties to
1.2% at the sour ones.
The pleasant apple flavor is owed to the many
essential oils that are available in apples. Different tastes of
individual fruit species are dependent on the ratio of the
quantity of sugars and organic acids. Interestingly, the
vitamin C content is different with different varieties of
apples, such as certain sorts may contain up to 3 times more
Vitamin C than the others.
Apples combine flavonoids, fibers and antioxidants
in unique and incomparable way. The greatest amount of
antioxidants contains in fully ripe fruits. Research conducted
at the University of Innsbruck, Austria, shows that with the
full ripening of apples, just before they spoil their
antioxidant levels increase.
Red and Golden Delicious are among the sweetest
varieties, Gray Smith and Gravenstein are the tartness and
during the processing retain their best texture.
The phytonutrient content of apple did not change
notably throughout the period of their storage. After 100
days, the content of phenolic compounds decreased slightly,
but even after 200 days kept cold, the overall amount of
these compounds remains about the same as it was in the
day of their retraction.
Nutritional values per 100 grams raw apple with
peel: energy - 218kj (50kal.) Carbohydrates - 13.81g., Starch
- 0.05g., Sugar - 10.39g., Sucrose - 2.07g., Glucose - 2.43g.,
Fructose - 5.9g., lactose - 0g., maltose - 0g., fiber - 2.4g., fat
- 0.17g., cholesterol- 0g., protein-0.26g., water-85.56g. ash -
0.19g[1].
Vitamins per 100 grams of raw apples cortex:
vitamin A - 0 μg (0%), alpha carotene - 0 μg (0%), beta-
carotene - 27μg (3%); Thiamine (B1) - 0,017mg (1%);
Riboflavin (B2) - 0.026 mg (2%); niacin (B3) - 0.091 mg
(1%); pantothenic acid (B5) - 0.061 mg (1%); Pyridoxine
(B6) - 0.041 mg (3%); folic acid (B9) - 3 μg (1%);
cobalamin (B12) - 0mg (0%); Vitamin C - 4.6 mg (5%);
vitamin D - 0 mg (0%); Vitamin E - 0.18 mg (1%); Vitamin
K - 2.2 μg (2%); lycopene - 0 μg (0%).
Minerals per 100 grams raw apple with peel:
calcium Ca - 6 mg (1%); Iron Fe - 0.12 mg (2%);
Magnesium Mg - 5mg (1%); Phosphorus P - 11 mg (2%);
potassium K - 107 mg (2%); sodium Na - 1 mg (0%); zinc
Zn - 0.04 mg (0%); Copper Cu - 0.027 mg (3%); Manganese
Mn - 0.035 mg (2%); Selenium Se - 0 mg (0%)[1].
Amino acids per 100 grams raw apple with peel:
tryptophan - 0,001g; threonine - 0,006g; threonine - 0,006 g;
isoleucine - 0,006g; leucine - 0,013g; lysine - 0,012g;
methionine - 0,001g; cystine - 0,001g; phenylalanine -
0,006g; tyrosine - 0,001g; valine - 0,012g; Arginine -
0,006g; histidine - 0,005g; alanine - 0,011g; aspartic acid-
0,07g; glutamic acid - 0,025g; glycine - 0,009g; proline -
0,006g; serine - 0,01g; choline - 0,003g[1].
Other ingredients in 100 g raw apple with peel:
alcohol - 0 g.
III. METHODS OF DETERMINATION
For the experiment using:
- 9 varieties of apples (Table. 1).
- Hipoknantin oxidase
- phosphate buffer
№ Variety apple Origin
1 Golden Delicious Katunitsa village
2 Golden Delicious Ognianovo village
3 Red Delicious Ognianovo village
4 Grey smith Ognianovo village
5 Mutsu Katunitsa village
6 Florina Katunitsa village
7 Chadeo Katunitsa village
8 Jonah Gold Katunitsa village
9 Aidere Katunitsa village
Table 1. Sorts of apples and place of cultivation
The experiment is based on the ability of antioxidants
(products with antioxidant properties) to trap free radicals -
super oxide anion (super oxide radical). The mechanism of
formation of the super oxide radical is the following:
Hypoxanthine + hypoxanthine oxidase + uric acid +
O2-super oxide radical
We evaluate the degree of antioxidant activity by the
difference in the degree of staining of the solution.
The degree of staining is determined by UV-VIS
spectrophotometer.
We determine the antioxidant activity by the
difference of absorption of the control sample and the
sample with the corresponding apple juice.
To determine the antioxidant activity of each apple
variety we use 3 tubes.
"0" - zero tube
"K" - a control tube
"1" - test tube
We obtain nectar from apple by squeezing them prior
the experiment using a PHILIPS ............. juicer.
To prepare the juice for the experiment we centrifuge
it for 5 minutes at 14000 rpm in centrifuge Centronix
Microcentrifugo 1236V. The goal is to settle the nectar
particles and to remain pure juice.
In all tubes we put in 1,5ml kit (hypoxanthine +
nitrobluetetrasole).
In tube, "0" we set 100μl sample (juice) + 400ml
buffer solution (phosphate buffer) instead of hypoxanthine.
In test tube "K" we put 400μl hypoxanthine oxidase
+100 ml buffer instead of sample.
3. International Journal of Technical Research and Applications e-ISSN: 2320-8163,
www.ijtra.com Volume 3, Issue 2 (Mar-Apr 2015), PP. 33-36
35 | P a g e
In test tube "1" we put 400μl hypoxanthine oxidase
+100 ml sample.
Or:
Tube "0" is used to remove the difference in color.
Tube "K" has the highest activity of free radicals.
Tube "1" has a lower activity of free radicals, as
some of them are caught by the sample.
Sequence of actions:
5. Mix the three tubes with electromagnetic stirrer
VORTEX GENIE 2 for 10 seconds.
6. Put the tubes in a water bath for 10-15 minutes at
380C.
7. Put the tubes in cold water for 5 seconds to stop
the reaction.
8. Determination of the activity of the samples with a
UV-VIS spectrophotometer
Thermo Scientific. Working length λ = 560 nm.
First we measure the control tube with maximum
activity.
For our 9 cases (9 varieties of apples) we measure 3
controls and take the average. The controls are the same.
We measure the absorbance of the nine samples "1".
For each we make two measurements and take the average.
(Table 2)
Before each measurement, we reset the apparatus
with a solution of test tube "0", to avoid loss of the
absorbance due to the coloration.
Table2. Absorption of test tube „1”
Measurement
1
Measurement
2
А
tube.1
ΔA = А мах.-А
tube.1
1 0.594 0.664 0.629 0.823
2 0.504 0.515 0.510 0.942
3 0.652 0.716 0.684 0.768
4 0.014 0.019 0.017 1.435
5 0.714 0.611 0.662 0.790
6 0.823 0.844 0.833 0.619
7 0.782 0.763 0.772 0.680
8 0.952 0.835 0.893 0.559
9 0.489 0.504 0.497 0.955
For antioxidant activity we judge by the change in
absorbance ΔA between tube "K" and tube "1".
Antioxidant activity (or SOD like activity) is
calculated by the following formula:
For 0,1 ml juice:
2ml is the full volume of the tube, and 0,1 – the
volume of the juice sample.
In each tube „К” and „1” we have the following
volume:
Amax – A of test tube “K”
ΔA - Amax – A of test tube “1”
In %:
Amax = 100%
And tube”1” = Х %, where Х are the percents of
absorption of the tube.
The subtraction 100 % - Х % results in antioxidant
activity measured in %, which is given respectively for each
variety in table 3.
Table3. Antioxidant activity of the apple sorts.
SOD
ANTIOXIDANT
ACTIVITY IN %
22.67 56.68
25.95 64.88
21.16 52.89
39.53 98.83
21.76 45.59
17.05 42.63
18.73 46.83
15.40 61.50
26.31 65.77
CONCLUSION
1. Choose ripe fruit to generate the highest amount of
antioxidants.
2. The studied varieties of apples can be arranged
according their antioxidant activity as follows: Grey
smith, Aidere, Golden Delicious, Jonah Gold, Golden
Delicious, Red Delicious, Chadeo, Mutsu, and
Florina
REFERENCES
[1] http://www.bb-team.org/articles/
[2] Umakoshi H, Tuan LQ, Morimoto et al. Superoxide
dismutase-like activity of liposomes modified with
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Average value 1, 4523 nm
The difference in the three
measurements should be no greater
than 30%
1 – 1,528 nm
2 – 1.450 nm
3 – 1.379 nm
4. International Journal of Technical Research and Applications e-ISSN: 2320-8163,
www.ijtra.com Volume 3, Issue 2 (Mar-Apr 2015), PP. 33-36
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