Chemistry of amino acids with their clinical applicationsrohini sane
A comprehensive presentation on Chemistry of Amino acids with their clinical applications for MBBS , BDS, B Pharm & Biotechnology students to facilitate easy- learning.
Amino acids are the building blocks of proteins and thus a code of life. This slide discusses about the structure, importance and various classifications of amino acids.
Chemistry of amino acids with their clinical applicationsrohini sane
A comprehensive presentation on Chemistry of Amino acids with their clinical applications for MBBS , BDS, B Pharm & Biotechnology students to facilitate easy- learning.
Amino acids are the building blocks of proteins and thus a code of life. This slide discusses about the structure, importance and various classifications of amino acids.
All proteins are formed of 20 amino acids.They are mainly formed of α amino acids (except proline).They have COOH and NH3 on same carbon atom. In physiological conditions both the groups are are completely ionised so an amino acid can act both as acid and base (amphoteric)
This Course is included in the syllabus of Bachelor in Science Agriculture level study in Tribhuvan University. The course belongs to 1h lecture.This slide include general introduction of amino acid. It describes about structure, function , type and role of amino acid.
Amino acids are biologically important organic compounds composed of amine (-NH2) and carboxylic acid (-COOH) functional groups, along with a side-chain specific to each amino acid. The key elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen, though other elements are found in the side-chains of certain amino acids. About 500 amino acids are known and can be classified in many ways. They can be classified according to the core structural functional groups' locations as alpha- (α-), beta- (β-), gamma- (γ-) or delta- (δ-) amino acids; other categories relate to polarity, pH level, and side-chain group type (aliphatic, acyclic, aromatic, containing hydroxyl or sulfur, etc.). In the form of proteins, amino acids comprise the second-largest component (water is the largest) of human muscles, cells and other tissues.Outside proteins, amino acids perform critical roles in processes such as neurotransmitter transport and biosynthesis.
All proteins are formed of 20 amino acids.They are mainly formed of α amino acids (except proline).They have COOH and NH3 on same carbon atom. In physiological conditions both the groups are are completely ionised so an amino acid can act both as acid and base (amphoteric)
This Course is included in the syllabus of Bachelor in Science Agriculture level study in Tribhuvan University. The course belongs to 1h lecture.This slide include general introduction of amino acid. It describes about structure, function , type and role of amino acid.
Amino acids are biologically important organic compounds composed of amine (-NH2) and carboxylic acid (-COOH) functional groups, along with a side-chain specific to each amino acid. The key elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen, though other elements are found in the side-chains of certain amino acids. About 500 amino acids are known and can be classified in many ways. They can be classified according to the core structural functional groups' locations as alpha- (α-), beta- (β-), gamma- (γ-) or delta- (δ-) amino acids; other categories relate to polarity, pH level, and side-chain group type (aliphatic, acyclic, aromatic, containing hydroxyl or sulfur, etc.). In the form of proteins, amino acids comprise the second-largest component (water is the largest) of human muscles, cells and other tissues.Outside proteins, amino acids perform critical roles in processes such as neurotransmitter transport and biosynthesis.
This presentation the chemical structure of natural amino acids. It also classifies amino acids according to several criteria e.g., structure (aliphatic, aromatic, and heterocyclic amino acids), reaction (Neutral, acidic and basic amino acids), polarity (polar and nonpolar amino acids), and metabolic fate ( glucogenic, ketogenic and glucoketogenic amino acids)
Table of Contents
What are Amino Acids?
Properties of Amino acids
Physical Properties
Chemical Properties
Structure of Amino acids
Classification of amino acids on the basis of R-group
Classification of amino acids on the basis of nutrition
Essential amino acids (Nine)
Non-essential amino acids (Eleven)
Classification of amino acids on the basis of the metabolic fate
Functions of Amino acids
Amino acids structure classification & function by KK Sahu sirKAUSHAL SAHU
INTRODUCTION
STRUCTURE
CLASSIFICATION OF AMINO ACIDS
ELEROCHEMICAL PROPERTIES
IONIZATION
TITRATION CURVE
NONSTANDARD PROTEIN AMINO ACIDS
NONPROTEIN AMINO ACIDS
DISTRIBUTION IN PROTEIN
ESSENTIAL AMINO ACIDS
FUNCTIONS
It contain more information about Amino acids and their structure. Then , contain both physical and chemical properties. Next Classification of amino acids based on nutritional requirements, based on metabolic fate, Position of NH2 group, etc.,
General structure of amino acid
Specific learning objective (SLO): Amino acid as Ampholytes (acid and base), Zwitter ions.
Classification of amino acid on the basis of side chain, chemical composition, Nutritional Requirement and metabolic fate.
Derived amino acids.
Optical properties of amino acids.
Acid-Base properties and Buffer characteristic.
Biological Important Peptides
Proteins based on nutritional value
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CRISPR-Cas9, a revolutionary gene-editing tool, holds immense potential to reshape medicine, agriculture, and our understanding of life. But like any powerful tool, it comes with ethical considerations.
Unveiling CRISPR: This naturally occurring bacterial defense system (crRNA & Cas9 protein) fights viruses. Scientists repurposed it for precise gene editing (correction, deletion, insertion) by targeting specific DNA sequences.
The Promise: CRISPR offers exciting possibilities:
Gene Therapy: Correcting genetic diseases like cystic fibrosis.
Agriculture: Engineering crops resistant to pests and harsh environments.
Research: Studying gene function to unlock new knowledge.
The Peril: Ethical concerns demand attention:
Off-target Effects: Unintended DNA edits can have unforeseen consequences.
Eugenics: Misusing CRISPR for designer babies raises social and ethical questions.
Equity: High costs could limit access to this potentially life-saving technology.
The Path Forward: Responsible development is crucial:
International Collaboration: Clear guidelines are needed for research and human trials.
Public Education: Open discussions ensure informed decisions about CRISPR.
Prioritize Safety and Ethics: Safety and ethical principles must be paramount.
CRISPR offers a powerful tool for a better future, but responsible development and addressing ethical concerns are essential. By prioritizing safety, fostering open dialogue, and ensuring equitable access, we can harness CRISPR's power for the benefit of all. (2998 characters)
India Clinical Trials Market: Industry Size and Growth Trends [2030] Analyzed...Kumar Satyam
According to TechSci Research report, "India Clinical Trials Market- By Region, Competition, Forecast & Opportunities, 2030F," the India Clinical Trials Market was valued at USD 2.05 billion in 2024 and is projected to grow at a compound annual growth rate (CAGR) of 8.64% through 2030. The market is driven by a variety of factors, making India an attractive destination for pharmaceutical companies and researchers. India's vast and diverse patient population, cost-effective operational environment, and a large pool of skilled medical professionals contribute significantly to the market's growth. Additionally, increasing government support in streamlining regulations and the growing prevalence of lifestyle diseases further propel the clinical trials market.
Growing Prevalence of Lifestyle Diseases
The rising incidence of lifestyle diseases such as diabetes, cardiovascular diseases, and cancer is a major trend driving the clinical trials market in India. These conditions necessitate the development and testing of new treatment methods, creating a robust demand for clinical trials. The increasing burden of these diseases highlights the need for innovative therapies and underscores the importance of India as a key player in global clinical research.
CHAPTER 1 SEMESTER V - ROLE OF PEADIATRIC NURSE.pdfSachin Sharma
Pediatric nurses play a vital role in the health and well-being of children. Their responsibilities are wide-ranging, and their objectives can be categorized into several key areas:
1. Direct Patient Care:
Objective: Provide comprehensive and compassionate care to infants, children, and adolescents in various healthcare settings (hospitals, clinics, etc.).
This includes tasks like:
Monitoring vital signs and physical condition.
Administering medications and treatments.
Performing procedures as directed by doctors.
Assisting with daily living activities (bathing, feeding).
Providing emotional support and pain management.
2. Health Promotion and Education:
Objective: Promote healthy behaviors and educate children, families, and communities about preventive healthcare.
This includes tasks like:
Administering vaccinations.
Providing education on nutrition, hygiene, and development.
Offering breastfeeding and childbirth support.
Counseling families on safety and injury prevention.
3. Collaboration and Advocacy:
Objective: Collaborate effectively with doctors, social workers, therapists, and other healthcare professionals to ensure coordinated care for children.
Objective: Advocate for the rights and best interests of their patients, especially when children cannot speak for themselves.
This includes tasks like:
Communicating effectively with healthcare teams.
Identifying and addressing potential risks to child welfare.
Educating families about their child's condition and treatment options.
4. Professional Development and Research:
Objective: Stay up-to-date on the latest advancements in pediatric healthcare through continuing education and research.
Objective: Contribute to improving the quality of care for children by participating in research initiatives.
This includes tasks like:
Attending workshops and conferences on pediatric nursing.
Participating in clinical trials related to child health.
Implementing evidence-based practices into their daily routines.
By fulfilling these objectives, pediatric nurses play a crucial role in ensuring the optimal health and well-being of children throughout all stages of their development.
The dimensions of healthcare quality refer to various attributes or aspects that define the standard of healthcare services. These dimensions are used to evaluate, measure, and improve the quality of care provided to patients. A comprehensive understanding of these dimensions ensures that healthcare systems can address various aspects of patient care effectively and holistically. Dimensions of Healthcare Quality and Performance of care include the following; Appropriateness, Availability, Competence, Continuity, Effectiveness, Efficiency, Efficacy, Prevention, Respect and Care, Safety as well as Timeliness.
Global launch of the Healthy Ageing and Prevention Index 2nd wave – alongside...ILC- UK
The Healthy Ageing and Prevention Index is an online tool created by ILC that ranks countries on six metrics including, life span, health span, work span, income, environmental performance, and happiness. The Index helps us understand how well countries have adapted to longevity and inform decision makers on what must be done to maximise the economic benefits that comes with living well for longer.
Alongside the 77th World Health Assembly in Geneva on 28 May 2024, we launched the second version of our Index, allowing us to track progress and give new insights into what needs to be done to keep populations healthier for longer.
The speakers included:
Professor Orazio Schillaci, Minister of Health, Italy
Dr Hans Groth, Chairman of the Board, World Demographic & Ageing Forum
Professor Ilona Kickbusch, Founder and Chair, Global Health Centre, Geneva Graduate Institute and co-chair, World Health Summit Council
Dr Natasha Azzopardi Muscat, Director, Country Health Policies and Systems Division, World Health Organisation EURO
Dr Marta Lomazzi, Executive Manager, World Federation of Public Health Associations
Dr Shyam Bishen, Head, Centre for Health and Healthcare and Member of the Executive Committee, World Economic Forum
Dr Karin Tegmark Wisell, Director General, Public Health Agency of Sweden
Antibiotic Stewardship by Anushri Srivastava.pptxAnushriSrivastav
Stewardship is the act of taking good care of something.
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
WHO launched the Global Antimicrobial Resistance and Use Surveillance System (GLASS) in 2015 to fill knowledge gaps and inform strategies at all levels.
ACCORDING TO apic.org,
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
ACCORDING TO pewtrusts.org,
Antibiotic stewardship refers to efforts in doctors’ offices, hospitals, long term care facilities, and other health care settings to ensure that antibiotics are used only when necessary and appropriate
According to WHO,
Antimicrobial stewardship is a systematic approach to educate and support health care professionals to follow evidence-based guidelines for prescribing and administering antimicrobials
In 1996, John McGowan and Dale Gerding first applied the term antimicrobial stewardship, where they suggested a causal association between antimicrobial agent use and resistance. They also focused on the urgency of large-scale controlled trials of antimicrobial-use regulation employing sophisticated epidemiologic methods, molecular typing, and precise resistance mechanism analysis.
Antimicrobial Stewardship(AMS) refers to the optimal selection, dosing, and duration of antimicrobial treatment resulting in the best clinical outcome with minimal side effects to the patients and minimal impact on subsequent resistance.
According to the 2019 report, in the US, more than 2.8 million antibiotic-resistant infections occur each year, and more than 35000 people die. In addition to this, it also mentioned that 223,900 cases of Clostridoides difficile occurred in 2017, of which 12800 people died. The report did not include viruses or parasites
VISION
Being proactive
Supporting optimal animal and human health
Exploring ways to reduce overall use of antimicrobials
Using the drugs that prevent and treat disease by killing microscopic organisms in a responsible way
GOAL
to prevent the generation and spread of antimicrobial resistance (AMR). Doing so will preserve the effectiveness of these drugs in animals and humans for years to come.
being to preserve human and animal health and the effectiveness of antimicrobial medications.
to implement a multidisciplinary approach in assembling a stewardship team to include an infectious disease physician, a clinical pharmacist with infectious diseases training, infection preventionist, and a close collaboration with the staff in the clinical microbiology laboratory
to prevent antimicrobial overuse, misuse and abuse.
to minimize the developme
2. Proteins
The amino acid sequence (also called primary structure) of a protein is
the order of the amino acids in the protein chain.
The sequence is always read from the to the C-terminus of
the protein.
For example:
-Lys-Val-Phe-Ala-Met-Cys-Leu-Leu-Arg-Val-COO-
Or (in one-lettercode): VFAMCLLRV
4. One amino acid
- Cα is at the heart of the amino acid
- Cα, C N and O are called backbone atoms
- R can be any of the 20 side chains
5. Amino Acids
Amino Acids are the building units of proteins. Proteins are polymers of amino
acids linked together by what is called “ Peptide bond” (see latter).
There are about 300 amino acids occur in nature. Only 20 of them occur in
proteins.
Structure of amino acids:
Each amino acid has 4 different groups attached to α- carbon ( which is C-atom next
to COOH). These 4 groups are : amino group, COOH gp,
Hydrogen atom and side
Chain (R)
R
6. • At physiological PH (7.4), -COOH gp is dissociated forming a negatively charged
carboxylate ion (COO-) and amino gp is protonated forming positively charged ion
(NH3
+) forming Zwitter ion
• N.B. Proline is an imino acid not amino acid (see latter)
Classification of amino acids
I- Chemical classification: According to number of COOH and NH2 groups i.e. according
to net charge on amino acid.
A- Monobasic, monocarboxylic amino acids i.e. neutral or uncharged:
R
7. Subclassification of neutral amino acids:
All structures are required (See structures in hand out)
1- Glycine R= H
2- Alanine R= CH3
3- Branched chain amino acids: R is branched such as in:
a - Valine R= isopropyl gp
b- Leucine R= isobutyl gp
c- Isoleucine R = is isobutyl
R is isobutyl in both leucine and isoleucine but branching is different: in leucine
→ branching occurs on γ carbon
in isoleucine→ branching occurs on β- carbon
4- Neutral Sulfur containing amino acids:
e.g. Cysteine and Methionine. What is cystin?
5- Neutral, hydroxy amino acids:
e.g. Serine and Threonine
8. 6- Neutral aromatic amino acids:
a- Phenyl alanine : It’s alanine in which one hydrogen of CH3 is substituted
with phenyl group. So it’s called phenyl alanine
b- Tyrosine: - it is P- hydroxy phenyl alanine
- it is classified as phenolic amino acid
c- Tryptophan: as it contains indole ring so it is classified as heterocyclic amino
acid
7- Neutral heterocyclic amino acids:
a- Tryptophan: contains indole ring
b- Proline: In proline, amino group enters in the ring formation being α-imino gp
so proline is an α-imino acid rather than α-amino acid
9. B- Basic amino acids: Contain two or more NH2 groups or nitrogen atoms that act
as base i.e. can bind proton.
At physiological pH, basic amino acids will be positively charged.
e.g.
a- Lysine
b- Arginine: contains guanido group
c- Histidine: is an example on basic heterocyclic amino acids
10. C- Acidic Amino acids: at physiological pH will carry negative
charge.
e.g. Aspartic acid (aspartate) and Glutamic acid (glutamate). see structures in hand out.
Aspargine and Glutamine: They are amide forms of aspartate and glutamate in which side
chain COOH groups are amidated.
They are classified as neutral amino acids.
11. II- Classification according to polarity of side chain (R):
A- Polar amino acids: in which R contains polar hydrophilic group so can forms hydrogen bond
with H2O. In those amino acids, R may contain:
1- OH group : as in serine, threonine and tyrosine
2- SH group : as in cysteine
3- amide group: as in glutamine and aspargine
4- NH2 group or nitrogen act as a base (basic amino acids ): as lysine, arginine and
histidine
5- COOH group ( acidic amino acids): as aspartic and glutamic .
B- Non polar amino acids:
R is alkyl hydrophobic group which can’t enter in hydrogen bonf formation. 9 amino acids are
non polar ( glycine, alanine, valine, leucine, isoleucine, phenyl alanine, tryptophan, proline and
methionine)
12.
13. III- Nutritional classification:
1- Essential amino acids: These amino acids can’t be formed in the body and so, it is
essential to be taken in diet. Their deficiency affects growth, health and protein
synthesis.
2- Semiessential amino acids: These are formed in the body but not in sufficient amount
for body requirements especially in children.
Summary of essential and semiessential amino acids:
Villa HM = Ten Thousands Pound
V= valine i= isoleucine l= lysine l= leucine
A = arginine* H= histidine* M= methionine
T= tryptophan Th= threonine P= phenyl alanine
*= arginine and histidine are semiessential
3- Non essential amino acids: These are the rest of amino acids that are formed in the body
in amount enough for adults and children. They are the remaining 10 amino acids.
14. IV- Metabolic classification: according to metabolic or degradation products of amino acids
they may be:
1- Ketogenic amino acids: which give ketone bodies . Lysine and Leucine are the only pure
ketogenic amino acids.
2- Mixed ketogenic and glucogenic amino acids: which give both ketonbodies and
glucose.These are: isoleucine, phenyl alanine, tyrosine and tryptophan.
3- Glucogenic amino acids: Which give glucose. They include the rest of amino acids. These
amino acids by catabolism yields products that enter in glycogen and glucose formation.
15. Amphoteric properties of amino acids: that is they have both basic and acidic groups and so
can act as base or acid.
Neutral amino acids (monobasic, monocarboxylic) exist in aqueous solution as “ Zwitter ion”
i.e. contain both positive and negative charge. Zwitter ion is electrically neutral and can’t
migrate into electric field.
Isoelectric point (IEP) = is the pH at which the zwitter ion is formed. e.g IEP of alanine is 6
Chemical properties of amino acids:
1- Reactions due to COOH group:
-Salt formation with alkalis, ester formation with alcohols, amide formation with amines and
decarboxylation
-2- Reactions due toNH2 group: deamination and reaction with ninhydrin reagent.
-Ninhydrin reagent reacts with amino group of amino acid yielding blue colored product. The
intensity of blue color indicates quantity of amino acids present.
16. The 20 amino acids
A Ala Alanine
C Cys Cysteine
D Asp Aspartic acid (Aspartate)
E Glu Glutamic acid (Glutamate)
F Phe Phenylalanine
G Gly Glycine
H His Histidine
I Ile Isoleucine
K Lys Lysine
L Leu Leucine
M Met Methionine
N Asn Asparagine
P Pro Proline
Q Gln Glutamine
R Arg Arginine
S Ser Serine
T Thr Threonine
V Val Valine
W Trp Tryptophan
Y Tyr Tyrosine
17. The 20 amino acids
The side chains, R, determine the differences in the structural and chemical
properties of the 20 ‘natural’ amino acids.
The 20 amino acids can, for example, be classified as follows:
Hydrophobic
Aliphatic Ala, Leu, Ile, Val
Aromatic Phe, Tyr, Trp, (His)
Hydrophilic
Polar Asn, Gln
Alcoholic Ser, Thr, (Tyr)
Charged Arg, Lys, Asp, Glu, (His)
Inbetween:
Sulfur-containing Met, Cys
Special Gly (no R), Pro (cyclic)
Several amino acids belong in more than one category.
18. •There are many ways to characterize the properties of amino acids. The ones
most useful and most commonly used are:
•Hydrophobicity
•Size
•Charge
•Secondary structure preference
•Alcoholicity
•Aromaticity
•And on top of that there are some special characteristics like bridge forming
by cysteines, rigidity of prolines, titrating at physiological pH of histidine,
flexibility of glycines, etc.
26. • amino acids don’t fall neatly into classes--they are
different combinations of small/large,
charged/uncharged, polar/nonpolar properties
• the properties of a residue type can also vary with
conditions/environment
Key points about the character of amino acid
side chains
27. Obviously, there are relations between the
physico-chemical characteristics of the amino
acids and their secondary structure
preference.