Seed borne mycoflora of selected forest trees

1. Seed Borne Mycoflora Of Selected
Forest Tree Species In Mudigere Region
Of Chikkamagaluru District
PRESENTED BY
ADRABI
PS173051
I.D.S.G. Govt. College, Chikkamagaluru

2. Introduction
A forest is a large area dominated by trees.
 According to the widely used Food and
Agriculture Organization definition, forests
covered 4 billion hectares (9.9×109 acres) (15
million square miles) or approximately 30 percent
of the world's land area.
Forests are the dominant terrestrial ecosystem of
Earth, and are distributed around the globe. Forest
are the main constituents of the natural vegetation
(Breitenbach 1963).

3.  For regeneration of a forest proper germination of
seeds subsequent establishment of the trees is
essential.
 Plants are normally propagated through seeds.
 seed pathology may be defined as the study of
seedborne disease and pathogens.
 Seed pathology includes the study of diseases and
deterioration caused by bacteria, fungi, nematodes,
viroids, and viruses, and physiological and
mechanical disorders.
 A number of ecological groups of fungi can be found
associated with seeds.

4.  Seeds of forest trees like others, carry numerous fungi
which are known to cause considerable damage to
spreading seeds and seedlings.
 The majority of the fungi recorded from seeds of
forest trees so far belong to the conidial states of
Ascomycetes.

5. AIMS AND OBJECTIVES
Aim :
 The present study is aimed to detect the different types
of mycoflora present in the selected forest tree seeds.
 This is useful to know how the pathogen get associated
with seeds.
Objectives :
• Collection of different seed samples from forest.
• Screening of seed borne mycoflora
• Isolation and identification of seed borne mycoflora.

6. Study Area :

7. Materials and methods
 Collection of seeds
 PDA (Potato Dextrose Agar) method
 Standard blotter method
 Isolation of seed mycoflora

8. Result and Discussion
• Details of seed borne Mycoflora of forest seeds are
given below. We have selected 6 plants species from
Mudigere forest region for the identification of seed
borne mycoflora.
• From this study we get 30 species of fungi. In that
some species are unique and some of them are
different.
• They are showed in below tables.

9. • Leucaena leucocephala
Sl. No. Name of the Fungi
1. Aspergillus niger
2. Cladosporium sphaerospermum
3. Cochliobolus hawaiiensis
4. Fusarium javanicum
5. Penicillium crysogenum

10. • Pterocarpus marsupium
Sl. No. Name of the fungi
1. Alternaria brassicicola
2. Aspergillus kanagawaensis
3. Cochlibolus bicolor
4. Cladosporium cladosporioides
5. Penicillium spore
6. Torula caligans

11. • Gmelina arborea
Sl. No. Name of the fungi
1. Alternaria alternata
2. Aspergillus ochraceus
3. Spirodactylon aureum
4. Trichoderma citrinoviride
5. Trichothecium roseum

12. • Terminalia bellerica
Sl. No Name of the fungi
1. Aspergillus niger spores
2. Penicillium decumbens
3. Periconia species
4. Pithomyces maydicus
5. Trichoderma atroviride

13. • Neolamarckia cadamba
Sl. No. Name of the fungi
1. Aspergillus niger
2. Mucor varians
3. Myrothecium cinctum
4. Penicillium species
5. Periconia species

14. • Caryota urens
Sl. No. Name of the fungi
1. Aspergillus sydowii
2. Circinella species
3. Emericella nidulans
4. Penicillium species
5. Spegazzinia labulata

15. Based on classes the fungi are grouped and
represented in graphs :
Sl. No. Name of the classes No. of species
1. Ascomycetes 21
2. Dueteromycetes 6
3. Zygomycetes 3

16. 0
5
10
15
20
25
Ascomycetes Deutereomycetes Zygomycetes
Classes
Numberofspecies

17. Inoculated seed samples

18. Sample showing fungal growth

19. Different Fungal Species Obtained

24. Conclusion
To study the seed borne mycoflora we selected 6
forest trees from Mudigere region and we collected 6
different species. For the culture of fungi we
conducted both PDA method and blotter method. For
the identification of fungi we used compound
microscope with 40X and 10X magnification. We got
30 species of fungi.
The highest fungi growth was identified in PDA
method compared to blotter method.

25. By comparing the classes we get 70% 0f
Ascomycetes species, 20% of Deutereomycetes and
10% of Zygomycetes. In all the 6 seeds the
commonly identified fungi was Aspergillus species,
then 3 Penicillium species was obtained. Remaining
species were different from one another.
This seed borne mycoflora affect the seed
germination and young seedlings.
This study helps in the identification and suppression
of fungi in the collected seed samples and increases
the economic value of trees. By this project work, I
learned the culturing of fungi, indentification and we
can see variety of fungul species.

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