Genotypic variations in the inhibitory potentials of four combined botanicals on mycelia growth of Macrophomina phaseolina of cowpea (Vigna unguiculata (L) Walp.)
The ethanol extracts of Ficus asperifolia, Mormordica charantia, Anacardium
occidentals and Psidium guajava were evaluated sole and in treatment combinations at 25, 50 and
75mg ml-1 concentration levels against the mycelial growth of Macrophomina phaseolina of
Cowpea. The pathogen was cultured on plates containing botanicals amended Potato Dextrose
Agar (PDA) in three replicates while only ethanol treated PDA tested plates served the control
experiment. The radial growths were recorded at 4th, 6th and 8th day after inoculation. Data
obtained were analysed using the SAS software program version 9.2. The extract of Mormordica
charantia was the most effective in the botanical treatments alone. The most significant inhibition
of Macrophomina phaseolina were observed from the combined treatments of Ficus asperifolia,
Mormordica charantia and Anacardium occidentals (3.11 cm), followed by Mormordica
charantia and Psidium guajava (3.29 cm), then combination of four extracts; Ficus asperifolia,
Mormordica charantia, Anacardium occidentals and Psidium guajava (3.53 cm), then
Mormordica charantia and Anacardium occidentals (3.84 cm). Other treatments, either alone or in
combination produced significant result compared to the control experiment (6.94 cm). However,
the efficacy of botanicals increased with concentration and also significantly correlated with time
and reduction in mycelia extension of the pathogen. More so, variability in the antifungicidal
potentials of the botanicals on Macrophomina phaseolina ranges from 15.93% to 34.06%
according to Eigen proportions. The treatment combinations of; Ficus asperifolia, Mormordica
charantia and Anacardium occidentals at 75mg ml-1 concentration level produced the most
inhibitory effect against Macrophomina phaseolina in vitro. However, the untreated plates did not
show inhibitory effect on the mycelial growth of the pathogen. Therefore, combined treatments of
botanicals could be a potential source in the practice of plant disease control.
Genetic characterization of morphological and yield traits in ten genotypes of Celosia argentea L. was evaluated
at the Research Farm of the Department of Botany, University of Ibadan, Nigeria. The experiment was laid out
in a randomized complete block design with four replicates. The results of analysis of variance carried out on
early morphological characters of C. argentea L. at 3, 4, and 5weeks after sowing showed significant
(p<0.05 /><0.01) effects except for number of leaves per plant and leaf width at 3 and 5 weeks after sowing,
respectively. The replicates in blocks produced varying observable effects on the genotypes while genotype x
replicate showed significant variation on morpho-agronomic and yield traits except number of days to flowering
at 50 days and fruit length at maturity. Also, from the result of the mean separation, it is shown that
NG/MAY/09/015 performed the best for plant height at flowering, leaf length at flowering, leaf width at
flowering, and root biomass. NG/SA/07/213 produced the highest mean values of number of flowers per plant,
leaf biomass and pod weight at maturity. The highest values of number of primary branches and fruit length at
maturity (FLM) were observed for NG/TO/MAY/09/015, while NG/AO/MAY/09/015 had the highest for pod
weight at maturity. The result of principal component axis also showed that Prin 1 accounted for highest Eigen
Vector of 38.62% from the total variation. NG/MAY/09/015 (R2) genotype produced the highest Eigen Vector
of 6.705 from Prin 1. The correlation result showed that plant height had a significant positive association with
seed weight at maturity, pod weight at maturity, number of primary branches and fruit length at maturity, while
similar association existed between leaf biomass, number of primary branches and pod weight at maturity, as
well as between plant height at flowering and pod weight at maturity. Again, the number of primary branches is
also positive and significantly correlated with plant height, root biomass and leaf length. Furthermore, the
results of dendrogram and minimum spanning tree revealed variations in genetic relatedness and distance,
respectively, which exist among the population of the C. argentea L.
Sixteen genotypes of maize were evaluated for genetic variability and character associations in the Research
Farm of the Department of Botany, University of Ibadan, Nigeria for 90 days in the 2012, 2013 and 2014
cropping seasons. Perforated polythene bags (18 × 9 cm) were filled with 6kg sandy-loam soil and were spaced
at a distance of 10 cm apart between rows. Three seeds from each genotype were then planted per bag without
treatment in three replicates. Data on plant and stem height, leaf width, leaf length and number of leaves were
collected after planting at 14 days interval on each replicate for a particular genotype. The plant height of
EVDT.Y200STRQPM genotype was significantly (p < 0.05) different from other genotypes, while genotype
pairs; TZE-OR2DTSTRQPM and 2009TZE-ORIDTSTRQPM, TZEI 22, TZEI 98 and OBANTAPA, TZEI 4,
TZEI 161, BODIJA as well as EVDT-W99STR, OJO, 98SYNWECSTRQPM were similar to each other. The
result of correlation coefficient shows that stem height was highly significant and positively correlated with the
plant height (p < 0.01; r = 0.91). The genotype is negative and non-correlated with plant height, stem height, leaf
width and number of leaves, but positive and non-significantly associated with leaf length, replicate and week
after planting.
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...AI Publications
The roothairs are important components of the root for absorbing nutrients for plants and also secreting the plant-produced secretes. Morphology and their number are influenced by various environmental and internal factors and are regulated by them. Mycorrhizal fungi are established through the root and their presence in the root can affect root’s physical and chemical properties.The aim of this study was to evaluate the effect of the presence of the fungus in the roots on the characteristics of the capillary roots.In order to remove the effect of other microorganisms on the results of the experiment, this experiment was performed in vitro on the roots of the carrot secondary phloem tissue culture. In this experiment, the fungus could affect the characteristics of the root hairs: their number (22% decrease in the root hairs number in the root hair area of the root) and their length (A decrease of 21.3% in the length of capillary roots in mycorrhizal plants). These changes in the characteristics of capillary roots were also caused by the presence of fungal structures in the roots as well as by the decrease in the production of strigolactones. In this study, changes in the production of strigolactones calculated by using their effect on seed germination of Phelipancheaegyptiaca.
Estimation of genetic parameters and gene actions of sorghum [Sorghum bicolor...Innspub Net
A study of sorghum tolerance to low P condition was conducted in two experiments : (1) in nutrient solution and
(2) in acid soil. The aim of this study was to obtain information on the genetic variability and heritability of agronomic characters that could be used as selection criteria in low P condition and to obtain information on gene action that controlled agronomic characters of sorghum.The plant materials used in this experiment were F2 segregating population derived from the cross between B69 and Numbu. The first experiment was conducted in the green house of Bogor Agricultural University. B69, Numbu and F2 population were grown hidroponically for 14 days. Composition of the nutrient solution used in the experiment followed the method suggested by Ohki with modification of P concentration. P is given in the form of KH2PO4: 0.001 mM. The results showed that shoot dry weight (SDW) and total dry weight (TDW) had high estimate of heritability and moderate genotypic
coefficient of variance (GCV), thus these characters can be used for criteria selection for low P tolerance at seedling stage. The significant value of skweness from the distribution shoot dry weight in F2 segregants, indicate the presence of complementary epistasis gene action, whereas normal distribution of total dry weight showed additive gene action. The second experiment was conducted in acid soils of Bogor District Experimental Field Tenjo, Bogor. The results showed that stem diameter, panicle weight and grain yield per plant had high heritability and wide GCV value, indicating these characters can be used as the selection criteria in selecting
sorghum genotypes for low P tolerance in acid soil. Based on the skewness values, all of the agronomy characters
observed in this study were influenced by additive gene action. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
Analysis of some Capparis L. accessions from Turkey based on IRAP and seed pr...Agriculture Journal IJOEAR
Abstract— 15 accessions from 10 different grid square of Turkey were analysed based on IRAP and seed protein patterns in order to observe the genetic diversity in the gene pool of Capparis. High levels of polymorphisms were detected with IRAP primers (93%) and seed protein electrophoresis (55.5%). Specific delineation between C. spinosa and C. ovata, and segregations of the accessions related to infraspecific status and eco-geographical distributions were presented in the dendrograms and PCA analysis. Significantly correlation between IRAP markers and seed protein profiles of the specimens was detected (p< 0.0001). Combination of genomic/proteomic marker systems may be useful approach for determining the broad genetic diversity in gene pool of Capparis, identification of the germplasms and ecologically tolerant genotypes in breeding programs.
Criteria for the Selection of Vegetable Growth-Promoting Bacteria to be appli...Agriculture Journal IJOEAR
In order to define which are the most important criteria for the selection of plant Growth-Promoting bacterial strains of the Hibiscus sabdariffa L. crop (Roselle), bacterial strains isolated from the roots of Roselle plants of two varieties (Creole and Spider) were used, collected in the community of Río de los Peces, municipality of Candelaria Loxicha, Oaxaca and seeds of the same varieties. To characterize the varieties, the following were determined: total germination percentage (TGP), germination speed (GS), the root length(RL), the stem length (SL), the dry root biomass (DRB), the dry stem biomass (DSB) and the chlorophyll content (CC). Three types of LED lamps were used to illuminate the seedlings. The seeds inoculated with cells of six selected bacterial strains were grown in a greenhouse to determine: the stem length (SL) at 3, 45 and 65 days after sowing (das). The treatments were distributed under a completely random design and comparison of means (Tukey, p = 0.05). The TGP, DSB and DRB parameters were not useful in the selection process of the strains that promoted plant growth to a greater degree. The GS and SL to be considered safe criteria or not, what is important is the relationship of what happens at the time of germination and development of the seedlings in the laboratory and greenhouse. The SL of the plants in the greenhouse showed differences between strains, but not regarding the control and also only observed in the first days of development (3 das). The CC did not prove to be a good selection criterion either. The lamp composed of 15% white light, 27% blue light and 58% red light was the one that most promoted root growth.
The use of plants extracts in the improvement of cowpea yield at dang (Ngaoun...Innspub Net
In the substitution of chemical insecticides with potential biopesticides, the efficiency of Lippia multiflora, Plectranthus glandulosus and Callistemon rigidus were evaluated on the improvement of cowpea (Vinia unguiculata) in Dang (Ngaoundere, Cameroon). The experiment was conducted in a completely randomized block design with 5 treatments repeated 4 times each: the negative control, the positive control (Decis), L. multiflora, P. glandulosus and C. rigidus. These different insecticides were sprayed on cowpea plants with 14 days interval starting from the 14th day after sowing. The parameters collected were the diversity of insect pests, the number of pods, and the dry weight of the grains. All of the insecticides used improved cowpea yields. Although less efficient than Decis, most biopesticides significantly (p < 0.001) improved the yield of cowpeas compared to the negative control. This improvement was 260% for C. rigidus, and 120% for P. glandulosus. These results suggest that C. rigidus, P. glandulosus and L. multiflora could be considered as potential substitutes for chemical insecticides in improving cowpea yields.
assessment of drumstick tree (m. deifera) accessionsIJEAB
An experiment was conducted to analyze the genetic diversity among 9 drumstick tree (Moringaoleifera) accessions in the Teaching and Research Farm of the University of Agriculture Makurdi. The experiment was laid out in a Randomized Complete Block Design (RCBD) replicated three times. Data were recorded on growth and yield characteristics before and after pruning. The result obtained showed that at 18 weeks after transplanting, accession UAM-NI had the tallest plants (3.63m) while UAM-BE had the shortest mean plant height (2.84m) under no pruning. Other parameters that showed significant differences were number of leaves per tree and stem diameter. Although accession UAM-OY recorded highest fresh (220.22g), dry (113.42g) and leaf powder (82.60g) weights, it was not significantly different from other accessions. However, at 18 weeks after pruning, there was a significant difference among the accessions with regard to leaf length. Although accession UAM-NA recorded highest fresh leaf weight (286.60g), dry leaf weight (90.67g) and leaf powder weight (85.60g), it was not statistically different from other accessions. For the pruned accessions, significant differences were recorded in leaf length, number of flowers/tree, days to podding and fifty percent podding, pod length, pod girth, pod weight, number of seeds/pod, number of seeds/tree and 100seed weight. The result also indicated that the pruned accessions recorded higher leaf yield than the unpruned. The result of the cluster analysis grouped the accessions into two clusters and an outlier both for the pruned and unpruned accessions irrespective of area of collection.
Genetic characterization of morphological and yield traits in ten genotypes of Celosia argentea L. was evaluated
at the Research Farm of the Department of Botany, University of Ibadan, Nigeria. The experiment was laid out
in a randomized complete block design with four replicates. The results of analysis of variance carried out on
early morphological characters of C. argentea L. at 3, 4, and 5weeks after sowing showed significant
(p<0.05 /><0.01) effects except for number of leaves per plant and leaf width at 3 and 5 weeks after sowing,
respectively. The replicates in blocks produced varying observable effects on the genotypes while genotype x
replicate showed significant variation on morpho-agronomic and yield traits except number of days to flowering
at 50 days and fruit length at maturity. Also, from the result of the mean separation, it is shown that
NG/MAY/09/015 performed the best for plant height at flowering, leaf length at flowering, leaf width at
flowering, and root biomass. NG/SA/07/213 produced the highest mean values of number of flowers per plant,
leaf biomass and pod weight at maturity. The highest values of number of primary branches and fruit length at
maturity (FLM) were observed for NG/TO/MAY/09/015, while NG/AO/MAY/09/015 had the highest for pod
weight at maturity. The result of principal component axis also showed that Prin 1 accounted for highest Eigen
Vector of 38.62% from the total variation. NG/MAY/09/015 (R2) genotype produced the highest Eigen Vector
of 6.705 from Prin 1. The correlation result showed that plant height had a significant positive association with
seed weight at maturity, pod weight at maturity, number of primary branches and fruit length at maturity, while
similar association existed between leaf biomass, number of primary branches and pod weight at maturity, as
well as between plant height at flowering and pod weight at maturity. Again, the number of primary branches is
also positive and significantly correlated with plant height, root biomass and leaf length. Furthermore, the
results of dendrogram and minimum spanning tree revealed variations in genetic relatedness and distance,
respectively, which exist among the population of the C. argentea L.
Sixteen genotypes of maize were evaluated for genetic variability and character associations in the Research
Farm of the Department of Botany, University of Ibadan, Nigeria for 90 days in the 2012, 2013 and 2014
cropping seasons. Perforated polythene bags (18 × 9 cm) were filled with 6kg sandy-loam soil and were spaced
at a distance of 10 cm apart between rows. Three seeds from each genotype were then planted per bag without
treatment in three replicates. Data on plant and stem height, leaf width, leaf length and number of leaves were
collected after planting at 14 days interval on each replicate for a particular genotype. The plant height of
EVDT.Y200STRQPM genotype was significantly (p < 0.05) different from other genotypes, while genotype
pairs; TZE-OR2DTSTRQPM and 2009TZE-ORIDTSTRQPM, TZEI 22, TZEI 98 and OBANTAPA, TZEI 4,
TZEI 161, BODIJA as well as EVDT-W99STR, OJO, 98SYNWECSTRQPM were similar to each other. The
result of correlation coefficient shows that stem height was highly significant and positively correlated with the
plant height (p < 0.01; r = 0.91). The genotype is negative and non-correlated with plant height, stem height, leaf
width and number of leaves, but positive and non-significantly associated with leaf length, replicate and week
after planting.
Effect of Clariodeoglomusclariodeorum on morphology and abundant of carrot ro...AI Publications
The roothairs are important components of the root for absorbing nutrients for plants and also secreting the plant-produced secretes. Morphology and their number are influenced by various environmental and internal factors and are regulated by them. Mycorrhizal fungi are established through the root and their presence in the root can affect root’s physical and chemical properties.The aim of this study was to evaluate the effect of the presence of the fungus in the roots on the characteristics of the capillary roots.In order to remove the effect of other microorganisms on the results of the experiment, this experiment was performed in vitro on the roots of the carrot secondary phloem tissue culture. In this experiment, the fungus could affect the characteristics of the root hairs: their number (22% decrease in the root hairs number in the root hair area of the root) and their length (A decrease of 21.3% in the length of capillary roots in mycorrhizal plants). These changes in the characteristics of capillary roots were also caused by the presence of fungal structures in the roots as well as by the decrease in the production of strigolactones. In this study, changes in the production of strigolactones calculated by using their effect on seed germination of Phelipancheaegyptiaca.
Estimation of genetic parameters and gene actions of sorghum [Sorghum bicolor...Innspub Net
A study of sorghum tolerance to low P condition was conducted in two experiments : (1) in nutrient solution and
(2) in acid soil. The aim of this study was to obtain information on the genetic variability and heritability of agronomic characters that could be used as selection criteria in low P condition and to obtain information on gene action that controlled agronomic characters of sorghum.The plant materials used in this experiment were F2 segregating population derived from the cross between B69 and Numbu. The first experiment was conducted in the green house of Bogor Agricultural University. B69, Numbu and F2 population were grown hidroponically for 14 days. Composition of the nutrient solution used in the experiment followed the method suggested by Ohki with modification of P concentration. P is given in the form of KH2PO4: 0.001 mM. The results showed that shoot dry weight (SDW) and total dry weight (TDW) had high estimate of heritability and moderate genotypic
coefficient of variance (GCV), thus these characters can be used for criteria selection for low P tolerance at seedling stage. The significant value of skweness from the distribution shoot dry weight in F2 segregants, indicate the presence of complementary epistasis gene action, whereas normal distribution of total dry weight showed additive gene action. The second experiment was conducted in acid soils of Bogor District Experimental Field Tenjo, Bogor. The results showed that stem diameter, panicle weight and grain yield per plant had high heritability and wide GCV value, indicating these characters can be used as the selection criteria in selecting
sorghum genotypes for low P tolerance in acid soil. Based on the skewness values, all of the agronomy characters
observed in this study were influenced by additive gene action. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
Analysis of some Capparis L. accessions from Turkey based on IRAP and seed pr...Agriculture Journal IJOEAR
Abstract— 15 accessions from 10 different grid square of Turkey were analysed based on IRAP and seed protein patterns in order to observe the genetic diversity in the gene pool of Capparis. High levels of polymorphisms were detected with IRAP primers (93%) and seed protein electrophoresis (55.5%). Specific delineation between C. spinosa and C. ovata, and segregations of the accessions related to infraspecific status and eco-geographical distributions were presented in the dendrograms and PCA analysis. Significantly correlation between IRAP markers and seed protein profiles of the specimens was detected (p< 0.0001). Combination of genomic/proteomic marker systems may be useful approach for determining the broad genetic diversity in gene pool of Capparis, identification of the germplasms and ecologically tolerant genotypes in breeding programs.
Criteria for the Selection of Vegetable Growth-Promoting Bacteria to be appli...Agriculture Journal IJOEAR
In order to define which are the most important criteria for the selection of plant Growth-Promoting bacterial strains of the Hibiscus sabdariffa L. crop (Roselle), bacterial strains isolated from the roots of Roselle plants of two varieties (Creole and Spider) were used, collected in the community of Río de los Peces, municipality of Candelaria Loxicha, Oaxaca and seeds of the same varieties. To characterize the varieties, the following were determined: total germination percentage (TGP), germination speed (GS), the root length(RL), the stem length (SL), the dry root biomass (DRB), the dry stem biomass (DSB) and the chlorophyll content (CC). Three types of LED lamps were used to illuminate the seedlings. The seeds inoculated with cells of six selected bacterial strains were grown in a greenhouse to determine: the stem length (SL) at 3, 45 and 65 days after sowing (das). The treatments were distributed under a completely random design and comparison of means (Tukey, p = 0.05). The TGP, DSB and DRB parameters were not useful in the selection process of the strains that promoted plant growth to a greater degree. The GS and SL to be considered safe criteria or not, what is important is the relationship of what happens at the time of germination and development of the seedlings in the laboratory and greenhouse. The SL of the plants in the greenhouse showed differences between strains, but not regarding the control and also only observed in the first days of development (3 das). The CC did not prove to be a good selection criterion either. The lamp composed of 15% white light, 27% blue light and 58% red light was the one that most promoted root growth.
The use of plants extracts in the improvement of cowpea yield at dang (Ngaoun...Innspub Net
In the substitution of chemical insecticides with potential biopesticides, the efficiency of Lippia multiflora, Plectranthus glandulosus and Callistemon rigidus were evaluated on the improvement of cowpea (Vinia unguiculata) in Dang (Ngaoundere, Cameroon). The experiment was conducted in a completely randomized block design with 5 treatments repeated 4 times each: the negative control, the positive control (Decis), L. multiflora, P. glandulosus and C. rigidus. These different insecticides were sprayed on cowpea plants with 14 days interval starting from the 14th day after sowing. The parameters collected were the diversity of insect pests, the number of pods, and the dry weight of the grains. All of the insecticides used improved cowpea yields. Although less efficient than Decis, most biopesticides significantly (p < 0.001) improved the yield of cowpeas compared to the negative control. This improvement was 260% for C. rigidus, and 120% for P. glandulosus. These results suggest that C. rigidus, P. glandulosus and L. multiflora could be considered as potential substitutes for chemical insecticides in improving cowpea yields.
assessment of drumstick tree (m. deifera) accessionsIJEAB
An experiment was conducted to analyze the genetic diversity among 9 drumstick tree (Moringaoleifera) accessions in the Teaching and Research Farm of the University of Agriculture Makurdi. The experiment was laid out in a Randomized Complete Block Design (RCBD) replicated three times. Data were recorded on growth and yield characteristics before and after pruning. The result obtained showed that at 18 weeks after transplanting, accession UAM-NI had the tallest plants (3.63m) while UAM-BE had the shortest mean plant height (2.84m) under no pruning. Other parameters that showed significant differences were number of leaves per tree and stem diameter. Although accession UAM-OY recorded highest fresh (220.22g), dry (113.42g) and leaf powder (82.60g) weights, it was not significantly different from other accessions. However, at 18 weeks after pruning, there was a significant difference among the accessions with regard to leaf length. Although accession UAM-NA recorded highest fresh leaf weight (286.60g), dry leaf weight (90.67g) and leaf powder weight (85.60g), it was not statistically different from other accessions. For the pruned accessions, significant differences were recorded in leaf length, number of flowers/tree, days to podding and fifty percent podding, pod length, pod girth, pod weight, number of seeds/pod, number of seeds/tree and 100seed weight. The result also indicated that the pruned accessions recorded higher leaf yield than the unpruned. The result of the cluster analysis grouped the accessions into two clusters and an outlier both for the pruned and unpruned accessions irrespective of area of collection.
Germination percentage and growing behavior of Salix tetrasperma (Willow) as ...Innspub Net
Propagation through branch cuttings is one of the best methods to produced tree nursery the yield of the tree produced from a cutting can be higher then a tree produced from seedlings, the conditions provided to them are important factor for getting good results. The aim of this study was to find the best size of cuttings for establishment of nursery and also to compare their performance in open air or in artificial conditions under plastic sheets cuttings of 2 inches, 4 and 6 of Salix tetrasperma were raised in plastic bags of size 3x7 their were three treatments with 25 bags in each and replicated 4 times. The data on sprouting percentage, plant height, root development etc was recorded after every two weeks. The data thus collected was analyzed statically using randomized complete block design. Result showed that cutting of 2 inches has high sprouting percentage and growth behavior as compared to other cuttings. Get more articles at: http://www.innspub.net/volume-6-number-4-april-2015-jbes/
Influence of water stress and rhizobial inoculation on growth and yield of se...Innspub Net
Two season’s field experiment and single season screen house experiment were conducted to assess the effect of water stress periods and rhizobial inoculation in five P. vulgaris cultivars. The experiment consisted of two levels of rhizobia (with and without inoculation), two stress levels (with and without water stress) and five cultivars of P. vulgaris (KAT B9, KAT B1, F9 Kidney Selection, F8 Drought line and JESCA). Results showed that rhizobial inoculation significantly increased plant height (cm), leaf area (cm2), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) at vegetative and flowering in field experiment. Furthermore, water stress treatments significantly reduced plant height (cm), stem diameter (mm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) in both growth stages at field experiment. For screen house experiment rhizobial inoculation significantly increased leaf area (cm2), number of leaves, stem girth (mm), shoot and root dry weight (g-1 plant) at both growth stages. Additionally, water stress treatments significantly reduced number of leaves, stem diameter (mm), shoot and root dry weight (g-1 plant) in both growth stages. Varieties F9 Kidney Selection, F8 Drought Line and JESCA had significantly superior measurements reflected in increased plant height (cm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) as compared with KAT B9 and KAT B1. Furthermore, significant interactive effects were also seen between rhizobial inoculation x stress level and tested bean cultivars on plant height, number of leaves, stem diameter, shoot dry weight and seed yields.
Evidence of natural hybridization and introgression between Medicago ciliaris...Agriculture Journal IJOEAR
Abstract—The present study, investigated some reproductive and fertility parameters in some wild populations, originating from the North Tunisia (4 populations of Medicago ciliaris and 3 populations of Medicago intertexta). Previous finding revealed that these species are genetically distinct and easily recognized by the number of flowers per inflorescence and pod dimensions. However, biometrical traits and isozyme patterns intermediacy between these two species had detected the existence of a potential spontaneous interspecific hybrid originating from Sedjnane locality in Tunisia. Indeed, the present work has shown significant decrease of pollen fertility and seed production for this population when compared to the others (pollen viability 75%, pollen germinability 8% and pod production=9%). These results suggested a possible natural interspecific hybrid and confirming introgressive hybridization possibility between M. intertexta and M. ciliaris.
Glyphosate resistance trait into soybean Cuban varieties: agronomical assessm...Innspub Net
Glyphosate-resistant (GR) soybean was one of the first major applications of genetic engineering in field crops and offered farmers a vital tool in fighting weeds. Weeds are a problem for soybean production in Cuba, so our work aim was the GTS 40-3-2 event introgression into Cuban varieties. Two local cultivars were crossed with transgenic genotypes that carry the event. From F1 to F3 generations, individual plants that produced more than 60 g of seeds per plant were chosen to obtain next generation. Individual lines were selected from F4 generation. F5 and F6 generations of five selected transgenic lines and their relatives were chosen to evaluate seven
agronomic traits throughout the summers of 2012 and 2013. A Random Block experimental design was done. First flowering (R1) and maturity (R8) stages of all genotypes were affected by planting date. Plant height of I1B2- 3, I1B4, I36B4 and RP5 lines ranged from 80 to 111 cm. I1B2-2 and I1B2-3 lines would be suitable for mechanized harvesting because they had the insertion of the first pod at 14.63 cm and 13.93 cm respectively. I36B4 line produced the greatest number of pods per plant (127). Transgenic lines produced more than 180 seeds per plant and 100-seed weight ranged from 13.75 g to 17.46 g. Seed yield per plant of transgenic lines and their parents IncaSoy36, CEB2 and CEB4 weren’t statistically different. These results could be a start point for other studies
involving larger areas, different planting dates and localities. Get more articles at: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Effect of interaction between different plant growth regulators on in vitro s...Agriculture Journal IJOEAR
— In this paper a shoot multiplication is described for Citrus latifolia Tan. (persian lime) using nodal segment explants of young one – old – year trees by two different pathways contain with and without callusing phase. The best result for multiple shoot formation and regenerated shoot formation was 3.2 and 2.6 shoots per explants with 4.44 µM BA plus 0.053 µM NAA and 4.44 µM BA plus 0.049 µM IBA respectively. Alike shoot regeneration, shoot elongation was occurred in medium with 4.44 µM BA and 0.049 µM IBA. Micropropagated and regenerated plants are under other experiments. Abbreviation: BA – 6 benzylaminopurine; IBA – Indole acetic acid; NAA – Naphtalene acetic acid; PGRs – Plant Growth Regulators.
Comparison of Growth and Development of Pleurotus florida against Wastes from...Dr. siddhant
Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the
substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165 gm, 33%) than egg shells (155 gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of
sporophores, both the substrates were statistically at par to each other. Utilization of human hairs egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.
Effects of 60Co gamma radiation doses on seed germination of Jatropha curcas ...Innspub Net
By: Baudouin K. Nyembo, Alexandre N. Mbaya, Calvin C. Ilunga, Jean-Louis N. Muambi, Luc L. Tshilenge
Key Words: Jatropha curcas, Gamma irradiation, Seed germination, LD50.
Int. J. Agron. Agri. Res. 13(5), 46-52, November 2018.
This study aimed to assess the effects of different gamma radiation doses from Cobalt -60 isotopic source on seed germination and early growth parameters of Jatropha curcas L. Healthy and dry seeds were subjected to three doses of gamma rays (100, 200 and 300 Gy). The experiment was conducted using randomized complete block design, with three replicates. The significantly maximum germination percentage (89.85 %), seedling survival (92.3 %), seedling collar diameter (0.892 cm), plant height (17.30 cm), number of leaves (7) were observed at 30 days after germination. The results revealed that seed germination percentages and seedling shoot length decreased with increasing dose of gamma-rays. Higher gamma-ray dose (300 Gy) in particular had a pronounced effect on these germination parameters than others, probably because high-dose inhibited cell division due to free radicals and DNA system damage. The LD50 for seeds germination rates was obtained at 254 Gy. These results implied that germination traits of Jatropha curcas seeds were sensitive to increase in gamma-ray.
Emerging issues and challenges associated with conservation of the African ba...Innspub Net
The aim of this review is to discuss the emerging issues and challenges in baobab conservation based on the climate change, emerging challenges related to herbivory and human use such as emerging of new markets for baobab products and land use changes.Specifically, the review summarizes scientific questions and challenges for the conservation of one of the most important indigenous fruit tree species, Adansonia digitata in Sub-Saharan Africa. It highlights on new information on the status of A. digitata in Tanzania with a strong focus on ethno-botanical issues, traditional and modern uses as well as challenges for the management and conservation of the tree species.We have done that by using facts from different parts of the geographical ranges of Africa and other parts of the world. The review is based on the belief that understanding the factors underpinning the use and management of A. digitata is an essential step towards development of credible strategies and plans that would advance and lead to sustainable management of baobabs.
Responses of wheat seedling to varying moisture conditions and relationship b...Agriculture Journal IJOEAR
— The following study was conducted to estimate the genotypic differences among 30 wheat (Triticum aestivum L.) genotypes under different moisture regimes and relationship between morphological and molecular characterization. Eight seedling parameters root length (RL), shoot length (SL), root fresh weight (RFW), shoot fresh weight (SFW), root dry weight (RDW), shoot dry weight (SDW), chlorophyll rate (CR) and survival rate (SR) were studied at four different soil moisture conditions (T 1 40%,T 2 60%,T 3 80%,T 4 100%) using two factor factorial complete randomized design (CRD). Significant differences among genotypes were observed by analysis of variance. For heritability estimates, survival rate showed lowest heritability under all the treatments. Principal components analysis accounted 81.4% variation in T 1 , 81.9% in T2, 87.7% in T3 and 84.7% in T4 conditions in first PC. Selected diverse genotypes were further fingerprinted with 10 ISSR markers. A total of 74 DNA fragments were detected and 72.7% of was polymorphic. The amplified DNA fragments were ranged from 4 (UBC-809) to 11 (UBC-808). PIC values were ranged from 0.32 to 0.81. Cluster analysis grouped the genotypes into 4 clusters on the basis of molecular and phenotypic characterization under T4 normal conditions whereas under T1 (moisture stress) conditions genotypes were grouped into 5 clusters explaining genotypic differences under different moisture conditions. The present results showed that phenotypic difference in wheat seedling expression under different water regimes is accompanied with molecular basis, which offer a prospective to enhance wheat adaptation under moisture stress conditions.
Evaluation of seed storage proteins in common bean by some biplot analysisINNS PUBNET
In order to study of seed storage proteins, proteins samples of common bean genotypes were prepared by 0.2 M
NaCl of extracting soluble. Genotypes were located in two groups by cluster analysis using Wilks’ lambda
statistic. Two groups were different for yield components (number of pods per plant, number of seeds per plant
and seed weight). Factor analysis showed that two factors described 61% of total proteins variation. Correlated
bands with yield components characters had the highest coefficients for the first factor. This factor was named
“yield components proteins”. Protein bands via RM 58 and 64 had relationship with days to flowering.
Therefore, the second factor was named “phenologic proteins”. Genotypes were located in four groups by these
factors. Length, angle and presence of protein bands were important characteristics to explain graphical
information in GGE biplot compared to factor analysis. Get the full articles at: http://www.innspub.net/volume-3-number-5-may-2013/
Germination percentage and growing behavior of Salix tetrasperma (Willow) as ...Innspub Net
Propagation through branch cuttings is one of the best methods to produced tree nursery the yield of the tree produced from a cutting can be higher then a tree produced from seedlings, the conditions provided to them are important factor for getting good results. The aim of this study was to find the best size of cuttings for establishment of nursery and also to compare their performance in open air or in artificial conditions under plastic sheets cuttings of 2 inches, 4 and 6 of Salix tetrasperma were raised in plastic bags of size 3x7 their were three treatments with 25 bags in each and replicated 4 times. The data on sprouting percentage, plant height, root development etc was recorded after every two weeks. The data thus collected was analyzed statically using randomized complete block design. Result showed that cutting of 2 inches has high sprouting percentage and growth behavior as compared to other cuttings. Get more articles at: http://www.innspub.net/volume-6-number-4-april-2015-jbes/
Influence of water stress and rhizobial inoculation on growth and yield of se...Innspub Net
Two season’s field experiment and single season screen house experiment were conducted to assess the effect of water stress periods and rhizobial inoculation in five P. vulgaris cultivars. The experiment consisted of two levels of rhizobia (with and without inoculation), two stress levels (with and without water stress) and five cultivars of P. vulgaris (KAT B9, KAT B1, F9 Kidney Selection, F8 Drought line and JESCA). Results showed that rhizobial inoculation significantly increased plant height (cm), leaf area (cm2), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) at vegetative and flowering in field experiment. Furthermore, water stress treatments significantly reduced plant height (cm), stem diameter (mm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) in both growth stages at field experiment. For screen house experiment rhizobial inoculation significantly increased leaf area (cm2), number of leaves, stem girth (mm), shoot and root dry weight (g-1 plant) at both growth stages. Additionally, water stress treatments significantly reduced number of leaves, stem diameter (mm), shoot and root dry weight (g-1 plant) in both growth stages. Varieties F9 Kidney Selection, F8 Drought Line and JESCA had significantly superior measurements reflected in increased plant height (cm), shoot and root dry weight (g-1 plant) and seed yields (kg-1 ha) as compared with KAT B9 and KAT B1. Furthermore, significant interactive effects were also seen between rhizobial inoculation x stress level and tested bean cultivars on plant height, number of leaves, stem diameter, shoot dry weight and seed yields.
Evidence of natural hybridization and introgression between Medicago ciliaris...Agriculture Journal IJOEAR
Abstract—The present study, investigated some reproductive and fertility parameters in some wild populations, originating from the North Tunisia (4 populations of Medicago ciliaris and 3 populations of Medicago intertexta). Previous finding revealed that these species are genetically distinct and easily recognized by the number of flowers per inflorescence and pod dimensions. However, biometrical traits and isozyme patterns intermediacy between these two species had detected the existence of a potential spontaneous interspecific hybrid originating from Sedjnane locality in Tunisia. Indeed, the present work has shown significant decrease of pollen fertility and seed production for this population when compared to the others (pollen viability 75%, pollen germinability 8% and pod production=9%). These results suggested a possible natural interspecific hybrid and confirming introgressive hybridization possibility between M. intertexta and M. ciliaris.
Glyphosate resistance trait into soybean Cuban varieties: agronomical assessm...Innspub Net
Glyphosate-resistant (GR) soybean was one of the first major applications of genetic engineering in field crops and offered farmers a vital tool in fighting weeds. Weeds are a problem for soybean production in Cuba, so our work aim was the GTS 40-3-2 event introgression into Cuban varieties. Two local cultivars were crossed with transgenic genotypes that carry the event. From F1 to F3 generations, individual plants that produced more than 60 g of seeds per plant were chosen to obtain next generation. Individual lines were selected from F4 generation. F5 and F6 generations of five selected transgenic lines and their relatives were chosen to evaluate seven
agronomic traits throughout the summers of 2012 and 2013. A Random Block experimental design was done. First flowering (R1) and maturity (R8) stages of all genotypes were affected by planting date. Plant height of I1B2- 3, I1B4, I36B4 and RP5 lines ranged from 80 to 111 cm. I1B2-2 and I1B2-3 lines would be suitable for mechanized harvesting because they had the insertion of the first pod at 14.63 cm and 13.93 cm respectively. I36B4 line produced the greatest number of pods per plant (127). Transgenic lines produced more than 180 seeds per plant and 100-seed weight ranged from 13.75 g to 17.46 g. Seed yield per plant of transgenic lines and their parents IncaSoy36, CEB2 and CEB4 weren’t statistically different. These results could be a start point for other studies
involving larger areas, different planting dates and localities. Get more articles at: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Effect of interaction between different plant growth regulators on in vitro s...Agriculture Journal IJOEAR
— In this paper a shoot multiplication is described for Citrus latifolia Tan. (persian lime) using nodal segment explants of young one – old – year trees by two different pathways contain with and without callusing phase. The best result for multiple shoot formation and regenerated shoot formation was 3.2 and 2.6 shoots per explants with 4.44 µM BA plus 0.053 µM NAA and 4.44 µM BA plus 0.049 µM IBA respectively. Alike shoot regeneration, shoot elongation was occurred in medium with 4.44 µM BA and 0.049 µM IBA. Micropropagated and regenerated plants are under other experiments. Abbreviation: BA – 6 benzylaminopurine; IBA – Indole acetic acid; NAA – Naphtalene acetic acid; PGRs – Plant Growth Regulators.
Comparison of Growth and Development of Pleurotus florida against Wastes from...Dr. siddhant
Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the
substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165 gm, 33%) than egg shells (155 gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of
sporophores, both the substrates were statistically at par to each other. Utilization of human hairs egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.
Effects of 60Co gamma radiation doses on seed germination of Jatropha curcas ...Innspub Net
By: Baudouin K. Nyembo, Alexandre N. Mbaya, Calvin C. Ilunga, Jean-Louis N. Muambi, Luc L. Tshilenge
Key Words: Jatropha curcas, Gamma irradiation, Seed germination, LD50.
Int. J. Agron. Agri. Res. 13(5), 46-52, November 2018.
This study aimed to assess the effects of different gamma radiation doses from Cobalt -60 isotopic source on seed germination and early growth parameters of Jatropha curcas L. Healthy and dry seeds were subjected to three doses of gamma rays (100, 200 and 300 Gy). The experiment was conducted using randomized complete block design, with three replicates. The significantly maximum germination percentage (89.85 %), seedling survival (92.3 %), seedling collar diameter (0.892 cm), plant height (17.30 cm), number of leaves (7) were observed at 30 days after germination. The results revealed that seed germination percentages and seedling shoot length decreased with increasing dose of gamma-rays. Higher gamma-ray dose (300 Gy) in particular had a pronounced effect on these germination parameters than others, probably because high-dose inhibited cell division due to free radicals and DNA system damage. The LD50 for seeds germination rates was obtained at 254 Gy. These results implied that germination traits of Jatropha curcas seeds were sensitive to increase in gamma-ray.
Emerging issues and challenges associated with conservation of the African ba...Innspub Net
The aim of this review is to discuss the emerging issues and challenges in baobab conservation based on the climate change, emerging challenges related to herbivory and human use such as emerging of new markets for baobab products and land use changes.Specifically, the review summarizes scientific questions and challenges for the conservation of one of the most important indigenous fruit tree species, Adansonia digitata in Sub-Saharan Africa. It highlights on new information on the status of A. digitata in Tanzania with a strong focus on ethno-botanical issues, traditional and modern uses as well as challenges for the management and conservation of the tree species.We have done that by using facts from different parts of the geographical ranges of Africa and other parts of the world. The review is based on the belief that understanding the factors underpinning the use and management of A. digitata is an essential step towards development of credible strategies and plans that would advance and lead to sustainable management of baobabs.
Responses of wheat seedling to varying moisture conditions and relationship b...Agriculture Journal IJOEAR
— The following study was conducted to estimate the genotypic differences among 30 wheat (Triticum aestivum L.) genotypes under different moisture regimes and relationship between morphological and molecular characterization. Eight seedling parameters root length (RL), shoot length (SL), root fresh weight (RFW), shoot fresh weight (SFW), root dry weight (RDW), shoot dry weight (SDW), chlorophyll rate (CR) and survival rate (SR) were studied at four different soil moisture conditions (T 1 40%,T 2 60%,T 3 80%,T 4 100%) using two factor factorial complete randomized design (CRD). Significant differences among genotypes were observed by analysis of variance. For heritability estimates, survival rate showed lowest heritability under all the treatments. Principal components analysis accounted 81.4% variation in T 1 , 81.9% in T2, 87.7% in T3 and 84.7% in T4 conditions in first PC. Selected diverse genotypes were further fingerprinted with 10 ISSR markers. A total of 74 DNA fragments were detected and 72.7% of was polymorphic. The amplified DNA fragments were ranged from 4 (UBC-809) to 11 (UBC-808). PIC values were ranged from 0.32 to 0.81. Cluster analysis grouped the genotypes into 4 clusters on the basis of molecular and phenotypic characterization under T4 normal conditions whereas under T1 (moisture stress) conditions genotypes were grouped into 5 clusters explaining genotypic differences under different moisture conditions. The present results showed that phenotypic difference in wheat seedling expression under different water regimes is accompanied with molecular basis, which offer a prospective to enhance wheat adaptation under moisture stress conditions.
Evaluation of seed storage proteins in common bean by some biplot analysisINNS PUBNET
In order to study of seed storage proteins, proteins samples of common bean genotypes were prepared by 0.2 M
NaCl of extracting soluble. Genotypes were located in two groups by cluster analysis using Wilks’ lambda
statistic. Two groups were different for yield components (number of pods per plant, number of seeds per plant
and seed weight). Factor analysis showed that two factors described 61% of total proteins variation. Correlated
bands with yield components characters had the highest coefficients for the first factor. This factor was named
“yield components proteins”. Protein bands via RM 58 and 64 had relationship with days to flowering.
Therefore, the second factor was named “phenologic proteins”. Genotypes were located in four groups by these
factors. Length, angle and presence of protein bands were important characteristics to explain graphical
information in GGE biplot compared to factor analysis. Get the full articles at: http://www.innspub.net/volume-3-number-5-may-2013/
Field experiments were conducted to investigate the variations in sixteen maize genotypes in relation to drought tolerance. The experimental set up was subjected to drought stress after five weeks of planting for three weeks before data on morphological and yield characters of maize genotypes were obtained for three cropping years. Plant height and grain yield of Bodija yellow maize were the highest overall. There was a significant difference among genotypes for drought stress resistance and Bodija yellow maize showed the most tolerance, while TZBR Comp 1 – C1 S2 510 genotype was the least. First principal component axis (Prin 1) had the highest contribution to the variation of the morphological, yield and drought tolerance traits. Prin 1 was highly related to the morphological and yield characters more than to the drought resistance. Plant height was negatively and strongly correlated (p<0.01) with stem height, number of leaves, stem girth, leaf length, leaf width and week after planting, but negatively correlated with the drought resistance. Therefore, Bodija yellow maize should be considered as parent material in breeding for the development of drought tolerant traits in maize.
The use of aluminium molds in injection molding is an emerging trend because of its ability to reduce cycle time by as much as 50% when compared to P20 tool steel.
Ultraviolet (UV) light has strong genotoxic effect to induce mutations for developing high genetic variability in yields, early maturity and other characters in crops. The study investigated the mutagenic effects of UV radiation on growth, yield, agronomic and mutation tolerance of six maize cultivars. Maize seeds were exposed to UV radiation, and planted in 7 kg soils in the polythene bags, while unexposed served as control. The effect of UV radiation on the first order interaction between weeks after planting (WAP) and treatments was only significant (p<0.05) height="true"><0.05)><0.05)><0.05)><0.01) with leaf length, leaf width and number of leaves with r = 0.95, 0.96, 0.89 respectively. Only the periods of exposure of the UV radiation was positive and strongly correlated with leaf width (r = 0.79). The association between the stover weight and periods of exposure was positive and insignificant, while the correlation between total numbers of grains and grain weight per stand was positive and strong (r = 0.99). Therefore, quality protein maize cultivars should be improved by introgression of favourable genes of drought tolerance, grain yield and related characters through induced mutation of UV radiation.
Michael Gangadeen is an attorney from Ozone Park, New York. He has been practicing the law since graduating with his Juris Doctor from St. John's University School of Law. Before starting his own practice in 2000, Michael provided legal representation to State Farm clients.
Similar to Genotypic variations in the inhibitory potentials of four combined botanicals on mycelia growth of Macrophomina phaseolina of cowpea (Vigna unguiculata (L) Walp.)
Potential of moringa for human health and moringa leaf extract as plant growt...Khairul Anam
Moringa is rich in several vitamins. It’s like growing multi-vitamins at your doorstep. Moreover , moringa leaf extract (MLE) is enriched with zeatin, a purine adenine derivative of plant hormone group cytokinin. It can be used as natural plant growth enhancer.
ANTIFUNGAL ACTIVITY OF SELECTED MEDICINAL PLANT EXTRACS AGAINST PLANT PATHOG...IJSIT Editor
The aim of this work was to find an alternative to chemical fungicides currently used in the control
plant pathogenic fungi Rhizoctoniasolani ,ColletotrichummusaeandFusariumoxysporum,. The antifungal
activity of the methanol extracts of six medicinal plants used in native medicine in Sri Lanka is reported.All
plant extracts were screened for their fungistatic, fungicidal activities and minimum inhibitory dilution (MID)
against above fungi. The media amended with methanol and recommended fungicide for respective fungal
strain were consider as negative and positive control respectively.Results showed that radial growth in all the
three tested organisms was significantly impaired (p<0.05) by the addition of the extracts in the culture
medium used. The test fungi differed in their reaction to the different extracts but on the whole, growth
inhibition increased with the concentration of each extract. The most active extracts, shows a marked effect of
the 20% methanol extracts from sweet flag with inhibition values of 91%, 86% and 84 % for F. oxysporum,R.
solani and C.muceawhereas those from wild basil inhibited the growth of the same pathogens by 89%, 84%
and 74%.The results showed minimal inhibitory concentrations (MIC) were 5 % (v/v) for sweet flag and wild
basil and 20% (v/v) for all other plant crude extracts. Out of six plants extract screened, wild basil and sweet
flag showed more than 80% fungal inhibition after 6 hour immersion and other extracts could not exceed
60% inhibition after any exposure time. The study revealed that methanol crude extract of sweet flag and
wild basil exhibit strong fungistatic and fungicidal activities against tested fungi. These results support the
potential use of these plant extracts in the management of diseases caused by tested plant pathogenic fungi.
4 ijfaf nov-2017-2-allelopathic effect of eucalyptusAI Publications
Laboratory and greenhouse experiments were carried out at the Faculty of Agricultural Sciences, University of Gezira, Sudan in season 2014/15 to study the allelopathic effects of Eucalyptus (Eucalyptus camaldulensis Dehnh) leaf on seed germination and seedling growth of some poaceous crops. Laboratory experiments were conducted to study the allelopathic effects of leaf aqueous extract of Eucalyptus on seed germination of sorghum (Sorghum bicolor [L.] Moench), millet (Pennisetum glaucum [L.] R. Br.), maize (Zea mays L.) and wheat (Triticum vulgare L.). Six concentrations (0, 20, 40, 60, 80 and 100%) of the leaf aqueous extract of Eucalyptus were prepared from the stock solution (50 g / l). Treatments, for each crop, were arranged in completely randomized design with four replicates. The seeds were examined for germination at three days after initial germination. Greenhouse experiments were conducted to study the allelopathic effects of Eucalyptus leaf powder on seedling growth of the same crops. The leaf powder of Eucalyptus was incorporated into the soil at rate of 0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0% on w/w bases in pots. Treatments, for each crop, were arranged in completely randomized design with four replicates. The experiments were terminated at 30 days after sowing and the plant height, number of leaves and root length of crop seedlings were measured as well as plant fresh and dry weight. Data were subjected to analysis of variance procedure. Means were separated for significance using Duncan`s Multiple Range Test at p 0.5. The results showed that the leaf aqueous extract of Eucalyptus significantly reduced seed germination of the tested poaceous crops and there was direct negative relationship between concentration and germination. Also, the results showed that incorporating leaf powder of Eucalyptus into the soil significantly decreased plant height, number of leaves and root length of crop seedlings as well as seedling fresh and dry weight. In addition, the reduction in seedling growth was increased as the leaf powder increased in the soil. Based on results supported by different studies, it was concluded that Eucalyptus has allelopathic effects on seed germination and seedling growth of the poaceous crops.
Identification and evaluation of antifungal compounds from botanicals for the...researchagriculture
Red rot is a devastating disease in sugarcane caused by fungus, Colletotrichum falcatum. In this study, eighteen different botanicals were screened for identifying effective antifungal compound against C. falcatum. Among the plants screened, 15 per cent aqueous leaf extract of Psoralea corylifolia alone inhibited 100 per cent growth of both mycelium as well as spore germination under in vitro conditions. The extract did not exhibit any inhibitory effect to the beneficial microbes viz., Pseudomonas fluorescens, Bacillus megaterium and Gluconacetobacter diazotrophicus which are normally used in sugarcane. The effective plant extracts exhibiting 100 per cent antifungal activity was subjected to TLC, HPLC and GC-MS analysis to identify the bioactive antifungal compound. It revealed the presence of 7H-furo [3,2-G] (1) benzopyran-7-one as main bioactive compound which is thought to be the intermediate of antifungal compound, 8 – methoxypsoralen formed during biosynthesis.
Article Citation:
Rajkumar D and Murugesan R.
Identification and Evaluation of Antifungal Compounds from Botanicals for the Control of Sugarcane Red Rot Pathogen, Colletotrichum falcatum.
Journal of Research in Agriculture (2013) 2(1): 164-172.
Full Text:
http://www.jagri.info/documents/AG0044.pdf
Identification and Evaluation of Antifungal Compounds from Botanicals for th...researchagriculture
Red rot is a devastating disease in sugarcane caused by fungus,
Colletotrichum
falcatum
. In this study, eighteen different botanicals were screened for
identifying effective antifungal compound against
C.
falcatum.
Among the plants
screened, 15 per cent aqueous leaf extract of
Psoralea corylifolia
alone inhibited 100
per cent growth of both mycelium as well as spore germination under
in vitro
conditions. The extract did not exhibit any inhibitory effect to the beneficial microbes
viz.
,
Pseudomonas fluorescens
,
Bacillus megaterium
and
Gluconacetobacter
diazotrophicus
which are normally used in sugarcane. The effective plant extracts
exhibiting 100 per cent antifungal activity was subjected to TLC, HPLC and GC
-
MS
analysis to identify the bioactive antifungal compound. It revealed the
presence of
7H
-
furo [3,2
-
G] (1) benzopyran
-
7
-
one as main bioactive compound which is thought to be
the intermediate of antifungal compound, 8
–
methoxypsoralen formed during
biosynthesis.
Allelopathic effects of weeds extract on seed germination of Trachyspermum co...Innspub Net
Seed germination stage is one of the most basic growth stages in medicinal plants that may be adversely affected by the chemicals released from other plants in the soil environment. The present study was performed to investigate the allelopathic effects of weeds, including (Xanthium strumarium, Amaranthus retroflexus and Chenopodium album) as common weeds in medicinal plant's farms, on seed germination parameters, total dry weight and allometry coefficient of Trachyspermum copticum L., Foeniculum vulgare and Cuminum cyminum L. in laboratory conditions. Treatments included extracts of all organs of three weed species, applied on the seeds of the medicinal plants at three concentrations of zero (distilled water), 50 and 100% by volume. The results showed that the extracts of mentioned weed species had a significant inhibitory effect on germination characteristics of the studied medicinal plants. The greatest negative impact on seed germination was recorded for the extract of Amaranthus retroflexus, but Trachyspermum copticum L. showed a better resistance to the extract of Amaranthus retroflexus as compared to Foeniculum vulgare and Cuminum cyminum. Get the full articles at: http://bit.ly/1NaHI5u
SYNERGISTIC ANTIMICROBIAL ACTIVITIES OF PHYTOESTROGENS IN CRUDE EXTRACTS OF T...lukeman Joseph Ade shittu
Intensive studies on extracts and biologically active compounds isolated from medicinal plants have doubled in the last decade worldwide. However, as a result of paucity of knowledge and folkloric claim on the effectiveness of sesame leaves in infectious disease treatments, we aimed to determine the synergistic antimicrobial activity of essential oils and lignans present in the crude leaves extracts of Sesame radiatum and Sesame indicum. Ethanolic, methanolic and aqueous extracts of both leaves were studied for their in-vitro synergistic antimicrobial activity against both Gram positive and Gram negative micro-organisms, and Yeast using Agar diffusion method. The GC-MS phytochemical screening of methanolic extract showed that the major compounds in essential oils are of carboxylic acids and phenolic groups especially, the most potent antioxidants known to man like sesamol, sesamolin and sesamin among others. Methanolic and ethanolic extracts have broad spectrum antimicrobial effect against all the tested pathogenic micro-organisms except Streptococcus pneumoniae and Staphylococcus aureus respectively, while the aqueous extract exhibited inhibitory activity on Staphylococcus aureus, Streptococcus pneumoniae and Candida albicans. The result confirmed the folkloric claims of the antimicrobial effectiveness of locally consumed sesame leaves extracts especially against bacterial and common skin infection in many areas of Nigeria .
A Study on the Removal of Pesticide Residues on Potatoes Using Moringa oleife...AI Publications
Moringa oleifera (MO) is a plant whose seeds possess strong coagulation properties capable of removing turbidity and heavy metals from water. In this study, the effectiveness of MO seeds as a potential pesticide removal apparatus for agricultural produce was investigated. The parameters of interest for each pesticide washing solution were pesticide-concentration reduction, cost, and taste alteration to the potatoes. Separate doses of 0.25 mL profenofos 500 EC were individually applied to distinct 250 g pesticide-free potato piles. A submersion of each tainted potato pile was carried out in one of the chosen pesticide baths (mineral water, 2% salt solution, 10% salt solution, vegetable bath wash, and 10 g MO seed) for 10 min, followed by a 10 second mineral water bath rinse. Remaining profenofos residue concentrations on potatoes were quantified using the quick, easy, cheap, rugged, and simple QuEChERs method coincided with High Performance Liquid Chromatography (HPLC). The experiments showed that the MO seed was the most effective at removing profenofos at 52.9% followed by the vegetable bath at 47.3%. Neither method caused an alteration in food taste, but the MO seed method is significantly cheaper at 2,000/L IDR ($0.14 USD) compared to the vegetable bath at IDR 10,668/L ($0.77 USD) at the time of the experiment, respectively.
This study was initiated in order to understand the biochemical mechanisms involved in the resistance of J. curcas to Lasiodiplodia theobromae. Artificial inoculations were done on young seedlings leaves of 8 J. curcas local accessions from Burkina Faso including 4 resistant and 4 susceptible. Two extractions were made for the evaluation of each biochemical parameter: one on the 1st day before and the second on the 7th day after inoculation. The study revealed that the response of J curcas to L. threobromae infection is depending to the resistance or the susceptibility of accessions. It showed an increase in the content of biomolecules synthesis such as phenolic, flavonoids, proteins, photosynthetic pigments, salicylic acid and also an increase of catalase and trypsin inhibition activities. In susceptible accessions, the study revealed a decrease in the photosynthetic pigments and an increase in MDA content comparatively to resistant accessions. The ACP performed on the basis of the evaluated biochemical parameters showed that the axis F1 allows the separation of the resistant and the susceptible accessions, and indicated that the resistance of accessions result in the activation of biomolecules synthesis such as phenolic, flavonoids, proteins, photosynthetic pigments, salicylic acid and stimulation of catalase and trypsin inhibition activities. The F2 axis is associated with sugars content and, chymotrypsin inhibition and SOD activities. In susceptible accessions, an increase in MDA content and SOD activity were observed. This study represents an important step in combating leaf fungal diseases by opting to the green technology and by breeding for genotypes with inducing natural defense compounds.
Effect of Chitosan on Disease Control and Yield Parameters of Rambutan (Nephe...AI Publications
Rambutan (Nephelium lappaceum L.) is an extensively grown fruit crop in Sri Lanka. Powdery mildew incited by fungus Oidium nephelii attack young leaves, flowers and immature fruits caused heavy fruit losses in Rambutan. Chitosan is a natural biodegradable de-acetylated form of chitin has been proven to control numerous pre and post-harvest disease on various horticultural commodities. Therefore, the objective of the present study was to investigate the effect of chitosan compounds on control of powdery mildew disease and yield parameters of Rambutan (Nephelium lappaceum L.) variety Malwana Special. Different types of chitosan i.e. chitosan fungicide, chitosan oligomer, copper containing chitosan and wettable sulphur were used as treatments. Based on the green house test results, 40 ppm concentration of chitosan fungicide, chitosan oligomer and Copper chitosan was identified as economically effective concentration for field studies. Field experiment was conducted in WU1 agro ecological zones at Gannoruwa in Central province of Sri Lanka. Experiment was arranged as Randomized Complete Block Design with 8 replicates. Treatments were sprayed by Knapsack sprayer. Disease severity Index of fruits, fruit weight and number of fruits per bunch were recorded at harvesting. Highest fruit weight was recorded in chitosan fungicide treated fruits. Higher number of fruits per bunch was recorded in all tested chitosan and wettable sulphur treated fruits compared to control. Chitosan fungicide (40ppm) and wettable sulphur 80 WP (4000ppm) has equally potential in controlling of powdery mildew pathogen of Rambutan variety Malwana Special.
The experiment was conducted at the experimental farm and laboratory of Institute of
Sustainable Agrotechnology, University Malaysia Perlis, Padang Besar, Perlis, Malaysia, with the objective
toinvestigate the inhibitory and stimulatory effects of aqueous extract of mungbean on seed germination and
seedling growth of three crop species, mungbean, sweet corn and okra. Different treatments of mungbean
aqueousextracts (vegetative fresh, vegetative after 2 weeks drying, vegetative after 4 weeks drying, flowering
fresh, flowering after 2 weeks drying, flowering after 4 weeks drying, flowering fresh, flowering after 2 weeks
drying, flowering after 4 weeks drying, maturity fresh, maturity after 2 weeks drying, maturity after 4 weeks
drying and water as control) were used to test their effect on the test species. The experiment was randomly
distributed and according to Completely Randomized Design(CRD) with five replicates. The results showed the
fresh vegetative aqueous extract of mungbean had a significant effect (stimulatory) on germination percent and
growth parameters such as number of root, root length and shoot height, of the three crop species. The study
revealed that the aqueous extract of mungbean have different effects (inhibitory and stimulatory) on the
seedlings and the mode of action depends on the associated plant species.Our results suggest that the aqueous
extract of mungbean from the different growth stages and drying periods have an allelopathic effect.
A high frequency microcloning protocol for subsequent cryopreservation in Kae...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Antibacterial and Antifungal Activities of Essential Oils of Crude Extracts o...lukeman Joseph Ade shittu
Concern about the rising prevalence of antibiotics-resistant strains of pathogenic microorganisms has been expressed in the last three decades. However, intensive studies on extracts and biologically-active compounds isolated from medicinal plants have also doubled in the last decade. As a result of paucity of knowledge and folkloric claim on the leaves effectiveness in infectious disease treatments, we aimed to determine the antimicrobial activity of essential oils and lignans present in the crude Sesame radiatum leaves extracts. Ethanolic, Methanolic and Aqueous extracts of Sesame radiatum leaves were studied for their in-vitro antimicrobial activity against both Gram positive and Gram negative micro-organisms and Yeast using Agar diffusion method. The GC-MS phytochemical screening of methanolic extract showed the presence of carboxylic acids and phenolic groups in essential oils especially some of the most potent antioxidants like Sesamol, Sesamolin and Sesamin. Both the methanolic and ethanolic extracts have broad spectrum antimicrobial effect against all the tested micro-organisms except Streptococcus pneumoniae, Candida albicans and Staphylococcus aureus respectively, while the aqueous extract exhibited no inhibitory effect on Staphylococcus aureus and Streptococcus pneumoniae except on Candida albicans. The result confirmed the folkloric claims of the antimicrobial effectiveness of locally consumed Sesame leaves extracts especially against bacterial and common skin infection in many areas of the Country (Nigeria).
Similar to Genotypic variations in the inhibitory potentials of four combined botanicals on mycelia growth of Macrophomina phaseolina of cowpea (Vigna unguiculata (L) Walp.) (20)
This study was carried out in Osogbo township of Osun State, Nigeria to isolate and determine prevalence and pathogenicity of microorganisms associated with deterioration of sweet orange fruits. Twenty samples of 20 infected and 20 non-infected sweet oranges (Citrus sinensis L) were collected from four open markets (Akindeko, Igbonna, Oja-Oba and Sabo markets) each. The samples were transported immediately to Fountain University Microbiology Laboratory for pathogenic analysis. The oranges were rinsed with distilled water and serially diluted in 10 folds. The highest three dilutions were considered for microbial count analysis. Each of the orange was cut and the liquid content inoculated on nutrient agar and potato dextrose agar, incubated at 370C and 250C respectively. They were observed for seven days, and the different colonies isolated using the slide culture technique. Biochemical analyses of the culture showed that Apergillus spp, Staphylococcus spp, Escherichia spp, Rhizopus spp and Shigella spp had the highest load. Pathogens prevalence revealed that Staphylococcus spp had highest (12.63%) at Sabo, 4.94% at Igbonna and 10.43% at Akindeko. Aspergillus spp with 6.60% and 17.58% loads were identified at Sabo and Oja-Oba respectively. Rhizopus spp had 21.97% at Oja-Oba, E. coli, 17.58% at Igbonna and Shigella spp, 8.24% at Akindeko. Rhizopus spp and Aspergillus spp were the most active microbes with respective 100% and 90% infections, while the least active microbes were Staphylococcus spp and Shigella spp. Harvesting fruits at the suitable periods and stored the harvested orange fruits under controlled conditions could aid in retarding the microbial growth of post-harvest spoilage of pathogenic microorganisms.
This study was carried out to isolate and identify pathogenic microorganisms associated with
deterioration of tomato fruits. Fruit samples of infected and non-infected tomatoes were collected from
two open markets, Oja-Oba and Sabo in Osogbo, Nigeria. Each of the tomato was cut and the liquid
content inoculated on nutrient agar and potato dextrose agar, incubated at 37 0C and 25 0C, respectively,
and observed from 24 hours to 5 days, after which different colonies obtained were identified using slide
culture technique. Two bacteria, Staphylococcus aureus and Bacillus spp, as well as two fungi Aspergillus
flavus and Rhizopus stolonifer were observed in the tomato samples in both markets. Prevalence indices
revealed that isolated pathogens is higher at Sabo market than Oja-Oba market. Pathogenicity tests also
revealed that both of bacteria and fungi caused fruit decay. Consumers’ awareness on potential health
hazards of consuming relatively cheaper and pathogen contaminated spoilt fruits should be intensified.
A field experiment was conducted to evaluate the effects of time of application and rates on the
performance of performance of okra (Abelmoschus esculentus L.) on July and November 2013 at Lapai (9o
2' N and 6o34'E) and Mokwa (9o 8'N and 5o4'E) in the southern Guinea savanna ecology of Nigeria, The
experiments consisted of three (3) levels of poultry manure application time (two weeks before planting, at
planting and two week after planting) and four (4) levels of poultry manure application rates(0, 5, 10 and 15
t ha-1). The experiments were laid out as a 3 x 4 factorial in a randomized complete block design. Data
collected include plant height, number of leaves, leaf area, number of branches fruit weight, pod length, pod
diameter and cumulative yield. The result showed that the application of poultry manure two weeks before
planting significantly produced taller okra plants, higher number of leaves, wider leaf area and more okra
branches than other treatments in the two locations in 2013 cropping season. Fruit characteristic results
followed the same trends. In the case of the poultry manure rate, the application of 10 t ha-1significantly
produced taller okra plant, higher number of leaves, wider leaf area and more okra branches than other
treatments in the two locations in 2013 cropping season. The application of 15 t ha-1 was comparable to 10 t
ha-1 but significantly different from 5 t ha-1 and no application in the two locations in 2013 cropping season.
This result therefore recommended that poultry manure should be applied at least two weeks before planting
at 10 t ha-1
The first edition of this book ‘‘PLANT LIFE DIVERSITY’’ comprised of eleven Chapters which include the following: 1. The characteristics of soil environment, 2. The plant kingdom, 3. Thallophyta (Fungi, Algae, Bacteria and Lichens), 4. Phycophyta (Algae), 5. Mycophyta (Fungi), 6. Lichens, 7. Bryophyta, 8. Pteridophyta (Ferns), 9. Angiosperms and Gynmnosperms, 10. Forms and functional adaptation of plant to ecosystem and 11. Growth in plants. This book is meant for students of Pre-Degree, B.Sc., B.Sc. (Hons) and M.Sc. of Biological group such as Plant Sciences, Zoology, Plant Breeding and Bio-sciences.
Two separate experiments were conducted at the sereenhouse of the Unilorin Sugar Research Institute
(USRI) IIorin, to investigate the optimum concentration of a weak acid solution required for the presciption of cut
sugarcane stalks during hybridization. In the first experiment. stalks of two (2) sugarcane varieties (LS 1-047 and LS1057)
which were at the flowering. phase were immersed in three (3) eonecntrations(Blank, O.004M nnd 0.006M
respectively) of sulphurous acid (H2SO]) solution. In the second experiment, stalks of three (3) varieties viz: LSI-047,
LSI-057 and C06806 at the vegetative phase and those of LSI-047, LSI-050, LSI-054, LSI-057, B6609 and'C06806 at
the Dowering phase were also immersed in four (4) concentrations (i.e. Blank, 0.0021\1, 0.004M and O.OO(,M
respectively) of sulphurie acid (H2S04) solution.
Our results showed a rapid decline in the physiologienl activities of the sugarcanc stalks regardlcss of the
concentration and/or the source of the weak acid used as preservative solution. Flowering sequence was also disrupted
in the flowering stalks since none of the varieties could proceed to tile next ,phase, indicating that the stock solutions
were toxic to the test varieties. However, rooting and side shoot emergence (beginning from the riftll and ,cvcnth day
rcspeetively) were observcd ill the sugareanc stalks at both the vegetative and Oowerillg ph,lsl's or diiTercnl
eoneelllralion of the weak 1-1250 •. indicating Ih,1I the: i'nrlJ'ulal ions 'frolll 112SO., were less tOXIC {O ti,l'. l''" 1-::. d"'11 {I")';l'
or the 1-1)50. where the test varie{les nl:ilhl:r r()o(ed nor produce sid\' ,hm)l.
i\ILllllllgh 1110 (2) or the: \'arie:lIl'S (L,SI-O'17 ,md 1..'()ll~()I,) ,'ppe"rl'" III PUSs,'ss greater {,lkral":C: k.\cl I·.' Ii;.: .!,!!"<',.:l'>I, 1:';11' lil<.'>
exoLic judging by their overall performance.
Terminalia avicennioides is an important medicinal plant used to manage many pathological
conditions among most inhabitants of rural communities in Nigeria and many other African countries. It
belongs to family Combretaceae and found commonly growing in the savannah region of West Africa. The
parts of the plant mostly used for medicinal purpose are root and stem barks extracts, which are rich sources
of phytochemicals such as anthraquinone, saponins, steroids, tannins and terpenes among others. In folk
medicine, there are claims that the plant has been used to cure various diseases such as dental caries, skin
infections, sore and ulcer, syphilis, bloody sputum, ringworm infection, gastrointestinal helminthes and several
others. In this review, we have tried to provide bases for most of these claims at the instance of the results of
modern investigations on the biological activities of the plant extract. The possibility of employing the plant
extract in the field of green chemistry for probable application in biomedical industries was also discussed.
Keywords: Terminalia avicennioides, uses, phytochemicals, biological activities, nanoparticles
Genetic control and heterosis depend directly on genetic divergence among the parents in generating promising hybrids
required by plant breeders. The purpose of this study was to determine the relative importance of heterosis, combining
abilities, regression and correlation estimates in order to develop hybrid cultivars in maize. The 28 F1 hybrids obtained by
partial diallel cross of 8 inbreds in a randomized complete block design were evaluated at the Lower Niger River Basin
Authority, Oke-Oyi, Nigeria in three years. General (GCA) and specific combining abilities (SCA) produced significant (P
< 0.01) effects for all the characters, while non-additive genetic effects were predominant. The levels of heterosis for grain
yield varied widely among crosses, ranging from -16.83 to 9.76%. Positive and significant genotypic and phenotypic
correlations among grain yield and some related characters (days to anthesis and silking; plant and ear heights; number of
ears plant-1 and 1000 seed weight) showed that each character could be used indirectly to selection of grain yield. These
results also indicated that SCA was more effective than heterosis for describing hybrid performance. The regression of
actual hybrid characters on the expected hybrid characters, based on parental GCA values, was highly significant.
Regression analysis also showed that all the agronomic characters jointly contributed 19.4% to grain yield of maize. The
inbreds (Pop 66 SR and 2000 EV DT-Y STRC4) and crosses (Pop 66 SR x, 2000 EV DT-Y STRC4, Pop 66 SR x KU1409
and 9006 x KU1409) featured prominently with respect to better GCA × Year and SCA × Year effects with high heterotic
values for maize grain yield and associated characters. These inbreds could serve as donors to obtain early and short
statured hybrids with higher yield, while the crosses may be exploited and adapted to the Nigerian Savannas. This study
also affirmed that genotypic and phenotypic correlation coefficients as well as combining abilities, heterosis and regression
analyses were found to be suitable models for yield improvement in maize breeding.
Drought and nitrogen (N) tolerance quality protein maize (QPM) could serve as a succor for malnourishment in the Sub-Saharan Africa, and adoption should be intensified where resource poor farmers cannot afford N fertilizer and drought on maize at grain filling stage is frequent. This study compares the quality index, protein, tryptophan and lysine contents of normal extra-early drought-tolerant and their quality protein maize hybrids under sub-optimal and optimal soil N conditions. Four normal drought-tolerant and their respective QPM hybrids were planted under no (0 kg N ha-1), low (30 kg N ha-1) and optimal (90 kg N ha-1) fertilizations at Oke Oyi, Ilorin in the southern Guinea savanna of Nigeria in 2012 and 2013 cropping seasons. The trials were set up in a split plot arrangement with the N rates as main plot and the eight cultivars as sub-plots. Each plot within N levels was four-row, laid out in a randomized complete block design with four replications. The normal cultivars outyielded the QPM versions across N rates with no significance difference. There is a linear increase in all protein qualities with increase in N rates among QPM, while normal maize counterparts had a linear decline with increase in N fertilization. The QPM cultivars also maintained their endosperm protein qualities across N rates. Across N environments, the grain quality characters, such as crude protein, tryptophan, and lysine contents in grain, showed a significant negative relationship with grain yields, which were relatively much stronger under low-N stress. 99TY, TYEEC4, 99TYQ and 20SYNEEWQ have viable traits that could be explored for the development of maize varieties with good grain yield and better protein qualities to improve protein availability in maize based diets and feed for large population of man and livestock.
A comparative study on the response of six maize hybrids: two
hybrids each from the 1980s (8321-21 and 8425-8), 1990s (9801-11 and 9803-2)
and 2000s (0103-11 and 0103-15) to sub-optimal and optimal nitrogen fertilization
was conducted in Oke-Oyi, Nigeria. The trials were set up in a split plot with three
nitrogen levels (0, 30 and 90 kg N ha-1) as a main plot and six hybrids as sub-plots.
Significant interactions were observed between hybrid and N level for all
characters, with increasing in variation as the level of N decreased. Mean grain
yield reductions across eras were 73.8% at no-N and 32.6% at low-N, and those of
optimal-N fertilization were 34.3% and 15.7% for 1980s and 1990s genotypes
respectively. Depending on N treatment, grain yield varied from 0.67 to 4.89 t ha-1.
Kernel number was most severely reduced by N stress, but had positive and highly
significant (p≤0.01) correlation with grain yield at all N levels. Genetic gains in
grain yield were 42% (between 1980 and 2000 eras) and 9% (between 1990 and
2000 eras) under optimal-N fertilization. The two modern hybrids of 2000 era
(0103-11 and 0103-15) were outstanding for all the agronomic traits and leaf
chlorophyll concentration at all N levels. Improving traits associated with fertilizer
N response could accelerate rate of genetic gains in maize yields.
Development of drought tolerant maize cultivars is prerequisite to achieving stable grain yield in
drought–prone ecologies of Nigeria’s Guinea savanna. However, success has been limited mainly due
to lack of maize genotypes that show clear differences in response to well defined moisture deficit
condition. Two sets of drought tolerant (DT) maize germplasm were evaluated under screenhouse and
field conditions between 1999 and 2002. In the screenhouse study, performances of the genotypes
were compared under well-watered condition and moisture deficit imposed at different growth stages.
Under field conditions, the first set comprising 11 accessions along with a check were evaluated for 4
growing seasons while the second set which comprised 3 DT varieties were evaluated along with 2
check varieties using monthly plantings between April and August of 2001 and 2002, respectively. In
the first set, post anthesis moisture deficit significantly reduced grain yield by 25 to 73.5% in the open
pollinated varieties (OPVs) and by 20 to 64% in the hybrids. Grain yield under field conditions ranged
from 2.48 to 3.49, 2.82 to 3.73 and 3.58 to 4.76 tons/ha-1 for 1999, 2000 and 2001 full growing seasons,
respectively, and 2.03 to 2.50 tons/ha-1 for 2000 late growing season. In the second set, pre and post
anthesis moisture deficits reduced grain yield by 77.6 and 95.8%, respectively, of well watered
condition while in the field, grain yields in the genotypes were highest for plantings made in April and
July (1.90 - 2.5 t/ha), lowest for August (0.7 -1.8 t/ha) when moisture deficit coincided with
reproductive phase. Yield stability exhibited under moisture deficit and on the field by 8522-2, Oba
super 2 and AK9943-DMRSR in the first set as well as DT-SR-Y C0 and DT-SR-W C0 in the second set,
indicates their suitability either as cultivars per se or as potential source of DT alleles for
development of DT maize varieties for Nigeria’s savanna ecologies.
The comparative efficiency of organic and synthetic fertilizers were studied on maize and cowpea, two staple
Nigerian crops. Chemical fertilizer did not support yield as much as poultry manure (PM) or arbuscular mycorrhizal
fungi(AMF), individual or in a combined state. The combination of PM and AMF was the best application expressing
yield in form of shoot productivity. Glomus clarum was a good fertilizing candidate in this study
Twenty two genotypes of quality protein maize (QPM) and two local checks were assessed for their lysine and tryptophan levels, as well as grain yield characteristics at the Lower Niger River Basin Development Authority station, Oke-Oyi, Ilorin, Nigeria for three years (2009-2011). The results showed that the QPM genotypes and the standard checks varied from one another, with respect to crude protein, zein dry matter, zein crude, lysine and tryptophan. The best QPM hybrids for grain yield (Dada-ba, ART98-SW5-OB, ART98-SW4-OB and TZPB-OB had percentage lysine and tryptophan advantage of 34% compared with the local checks. These hybrids also out-yielded other genotypes with yield advantage of 10, 24 and 26% over the best inbred, open pollinated variety and the standard check respectively. However, grain yield showed positive association with all the characteristics except crude protein content. Kernel number per cob had maximum correlation with grain yield followed by kernel rows per cob, cob diameter and cob weight. The direct effect for crude protein was positive but the correlation was negative. Conclusively, the QPM hybrids that combined high yield with the essential amino acids could be tested in different savanna agro-ecologies to identify those that could be released to farmers, while the superior inbreds could be introgressed for further breeding programs.
Combining ability studies for maize grain yield and other agronomic characters were carried out using
ten open-pollinated maize varieties and their 45 F1 hybrids in a Striga hermonthica (Del.) Benth endemic
zone (Shonga) and non-endemic zone (Ilorin) in Kwara State, Nigeria, during the 2005 cropping season.
Both general combining ability (GCA) and specific combining ability (SCA) effects for Striga related
characters such as Striga shoot counts, syndrome ratings, flowering Striga shoots and barren maize
plants were generally low, suggesting the role of additive and dominant gene action in tolerance to S.
hermonthica (Del.) Benth. Parents Acr 94 Tze Comp5 and Tze Comp3 C2 had significant (p < 0.05)
positive GCA effects for grain yield and other agronomic characters in both Striga endemic and nonendemic
environments respectively. Crosses Tze Comp3 C2 x Hei 97 Tze Comp3 C4, Tze Comp3 C2 x
Acr 94 Tze Comp5 and Ak 95 Dmr - Esrw x Acr 94 Tze Comp5 had significant (p < 0.05) positive SCA
effects for grain yield only in Striga endemic environment. These parents and hybrids appeared to have
gene pools for S. hermonthica tolerance that can be manipulated and used to develop promising
hybrids for early maturity and high grain yield across the Southern Guinea Savanna ecology.
Development of drought-tolerant maize varieties with high and stable yields is very imperative as being affordable alternative to
many smallholder farmers. Drought-tolerant maize varieties belonging to two maturity groups (10 early and 10 late/intermediate) were evaluated
for yield and other related characters in the southern guinea savannah (SGS) of Nigeria for two years. Days to flowering were higher in
the second year than the first year. Consistent number of days (3 days) was recorded for anthesis-silking interval in both years. Plant and
ear heights are greater in 2008 than 2007. However, plant and ear aspects were fair in overall phenotypic appeal and grain yield was not
significantly difference in both years. Maize grain yield in late/intermediate varieties is significantly higher than the early with a difference of
one tonne. High grain yield recorded in two varieties each among the early (AC 90 POOL 16 DT STR and TZE-Y DT STR C4) and late/
intermediate (DT-SR-WC0 F2, SUWAN-1-SR-SYN) varieties was approximately 4.6 t/ha. These genotypes could be used either as cultivar
per se to escape the prolonged moisture stress during the later part of the cropping season or introgressed with favourable cultivars for high
yield adaptable to drought-prone areas in SGS ecologies.
Experiments were conducted on six newly developed open pollinated quality protein maize (QPM) genotypes and two
check entries for three years (2009-2011). The objective was to assess their yield potentials and disease tolerance/
resistance in the southern Guinea savanna agro-ecology of Nigeria. Genotype and year of evaluation were significant for
(P<0.01><0.05) for grain yield, harvest moisture and lodging characteristics. Genotypes x year interactive effect for
grain yield revealed different genotypic performance of the genotypes tested with two checks (Oba-Super 1 and DMRLSR-
Y) being responsible for the significant differences obtained in the three years of evaluation. Average grain yield was
significantly higher in the year 2011 compared to 2009 and 2010. All the genotypes tested were moderately tolerant to the
five diseases ranging from 1.5 (Streak virus) in Oba-Super 1 (check) to 2.9 (Southern leaf blight, Curvularia leaf spot and
Leaf rust) in the ART98-SW6-OB and ART98-SW4-OB respectively. Ear rot mostly affected the leaves among diseases
with a range of 2.3 to 2.8 in TZPB-OB and DMR-LSR-Y respectively.. Four QPM genotypes (ART98-SW5-OB,
ART98-SW4-OB, TZPB-OB and ART98-SW6-OB) were superior for grain yield with yield advantage of 28% over the
best OPV check. These QPM genotypes can therefore serve as useful replacement for existing cultivars and also as source
of genes for future maize breeding activities in the development of superior maize varieties with high protein contents for
the savanna agro-ecology.
Quality protein maize (QPM) combining the enhanced levels of
lysine and tryptophan with high grain yield and desirable agronomic traits could
reduce food insecurity and malnutrition in West and Central Africa. Twenty-two
varieties of QPM and two local checks were evaluated for agronomic
characteristics and nutritional qualities in the southern Guinea savanna of Nigeria
for two years (2009 and 2010). Though crude protein levels are good indicators of
quality, amino acid composition analyses, especially lysine and tryptophan that
would provide a final proof are in progress. The genotypes comprised five open
pollinated varieties (OPVs), nine inbred lines, eight hybrids and two local varieties.
Differences among the varieties were significant (P<0.01)><0.05) for days to tasselling. Most of the QPM inbred lines (CML
437, CML 490 CML 178) and hybrids (Dada-ba, ART98-SW5-OB, ART98-SW4-
OB and TZPB-OB) have superior performance for grain yield, other yield
attributes and nutritional qualities compared with the OPVs and local checks.
These inbreds could be potential sources of favorable alleles useful for future
maize breeding, while the hybrids could be evaluated in different environments of
Nigeria for comparative advantages in different environments and quality of the
grains to be released to farmers.
Field experiments were conducted at the University of Ilorin Teaching and Research Farm in 2005 and
2006 cropping seasons with the objective to evaluate the combining ability for maize grain yield and
other agronomic characters in 10 open pollinated maize varieties, which have been selected for high
yield and stress tolerance. General combining ability (gca) and year (y) effects were significant for all
the parameters except plant height, while specific combining ability (sca) and gca x year effects were
significant only for grain yield. However, Tze Comp4 Dmr Srbc2, Tze Comp4 C2 and Acr 94 Tze Comp5
which are good general combiners for maize grain yield, also showed positive significant gca x year
effects for flowering traits. Significant sca x year interaction effects were recorded for maize grain yield
and days to flowering, with Hei 97 Tze Comp3 C4 combining very well with 3 parents (Acr 90 Pool 16-Dt,
Tze Comp4-Dmr Srbc2 and Tze Comp4 C2). These parents and their hybrids probably have genes that
can be introgressed into other promising lines in developing early maturing and high yielding varieties
for cultivation in the Nigeria savannas.
This study was explored to determine the effect of the grain
yield and its related characteristics on 10 open-pollinated maize varieties and
their 45 F1 hybrids using correlation and path coefficient analysis. A two-year
study was conducted on maize genotypes at the University of Ilorin Teaching
and Research Farm Ilorin, Nigeria, during 2005 and 2006 growing seasons.
Positive and significant phenotypic and genotypic correlations were found for
days to 50% tasselling with plant and ear height, and grain yield with plant
height, number of grains ear-1 and ear weight. Positive and significant
environmental correlation was also recorded for grain yield with plant and ear
height, and ear weight. The path analysis revealed that, days to 50% silking,
ear weight and number of grains ear-1 had the highest direct effect on grain
yield, while number of grains ear-1 had the highest moderate indirect negative
effects on grain yield. Days to flowering, plant and ear height, number of grains
ear-1 and ear weight could be the important selection criteria in improving open
pollinated maize varieties and hybrids for high grain yield.
Change in climate and consequent global warming are posing threats to food security in many developing nations including Nigeria because of the climate-dependent nature of agricultural systems and lack of coping capabilities. The spatiotemporal pattern of temperature and rainfall of Nigeria between 1901 and 2005 (105 years), using temperature and rainfall data to support the concept of regional climate change and its impact on Agriculture and food security was in-vestigated. Also assessed was the level of agriculture funding and output in Nigeria. Mean annual air temperature and rain-fall data between 1901- 2005 (105 years) were obtained from Food and Agricultural Organization publications, National Bureau of Statistics, Central Bank of Nigeria bulletin and National Meteorological Agency. Descriptive statistics were used to analyze the data. The results showed that within the period of 105 years, rainfall decreased by 81 mm with increasing temperature of 1.1℃. The unpredictability of rainfall and steadily increasing air temperature were observed from 1971-2005. The total federal budget between 2001 and 2005 averaged 824 billion Naira per year of which very small amount (14.7 billion (1.8%)) went to agricultural sector. The actual spending was 681 billion Naira per year with 11.4 bil-lion Naira for agriculture. Over 25 years, there were low and dramatic walloping of public spending in agriculture relative to large size and importance of agricultural sector in the economy. There is a continuous rise in output from 1987 to 2000 before it dropped in 2001. Land degradation, desert encroachment, drying up of surface waters, coastal inundations, and shift in cultivated crops over time affected the food security in Nigeria. The current available data showed that Nigeria, like most parts of the world is experiencing the basic features of climate change. Therefore, Nigerian government/ private sector partnership should encourage agricultural, industrial and domestic practices which will not contribute to the emission of greenhouse gasses.
Reports of improvement in sweet corn (Zea mays saccharata) for grain yield and agronomic traits
through introgression of genes from the field corn (Zea mays L.) are common in literature. However, few
studies have reported improvement in field corn varieties for organoleptic and/or nutritional properties
through the transfer of recessive alleles that condition sweetness in sweet corn into the field corn. Field
and laboratory studies were conducted to determine changes in quantitative and qualitative
characteristics of three F1 hybrids derived by crossing sweet corn (pollen parent) unto field corn and
their F2 populations derived by sibmating approximately 200 ears from each of the F1 hybrids. Our
results showed significant changes in grain yield, grain protein concentration, carbohydrate and
calcium contents in the F1 hybrids and F2 populations. One hybrid (Pop31DMR x Sweet corn) had 21%
grain yield advantage over the maternal parent while another hybrid (Obatanpa x Sweet corn) showed
superiority for grain protein (27.3%) with corresponding lower carbohydrate content. Grains obtained
from the F2 populations had lower seed protein content than their respective maternal parents or the F1
hybrids and with corresponding higher carbohydrate content. Percent (%) loss in grain protein in the F2
seeds was higher than for other nutrient elements. Modest heterotic response was recorded for grain
yield in Pop31DMR x Sweet corn cross and for grain protein concentration in Obatanpa x Sweet corn
cross.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Genotypic variations in the inhibitory potentials of four combined botanicals on mycelia growth of Macrophomina phaseolina of cowpea (Vigna unguiculata (L) Walp.)
1. www.tropicalplantresearch.com 257
Received: 25 August 2015 Published online: 31 December 2015
ISSN (E): 2349 – 1183
ISSN (P): 2349 – 9265
2(3): 257–263, 2015
Research article
Genotypic variations in the inhibitory potentials of four combined
botanicals on mycelia growth of Macrophomina phaseolina of
cowpea [Vigna unguiculata (L) Walp.]
A. O. Akanmu1
, O. J. Olawuyi1
, O. B. Bello2
*, O. A. Akinbode3
,
T. Aroge1
, B. Oyewole1
and A. C. Odebode1
1
Department of Botany, P.O. Box 128, University of Ibadan, Ibadan, Nigeria
2
Department of Biological Sciences, Fountain University, Osogbo, Nigeria
3
Plant Pathology Unit, Institute of Agriculture Research and Training, Apata, Ibadan, Nigeria
*Corresponding Author: obbello2002@yahoo.com [Accepted: 07 December 2015]
Abstract: The ethanol extracts of Ficus asperifolia, Mormordica charantia, Anacardium
occidentals and Psidium guajava were evaluated sole and in treatment combinations at 25, 50 and
75mg ml-1
concentration levels against the mycelial growth of Macrophomina phaseolina of
Cowpea. The pathogen was cultured on plates containing botanicals amended Potato Dextrose
Agar (PDA) in three replicates while only ethanol treated PDA tested plates served the control
experiment. The radial growths were recorded at 4th
, 6th
and 8th
day after inoculation. Data
obtained were analysed using the SAS software program version 9.2. The extract of Mormordica
charantia was the most effective in the botanical treatments alone. The most significant inhibition
of Macrophomina phaseolina were observed from the combined treatments of Ficus asperifolia,
Mormordica charantia and Anacardium occidentals (3.11 cm), followed by Mormordica
charantia and Psidium guajava (3.29 cm), then combination of four extracts; Ficus asperifolia,
Mormordica charantia, Anacardium occidentals and Psidium guajava (3.53 cm), then
Mormordica charantia and Anacardium occidentals (3.84 cm). Other treatments, either alone or in
combination produced significant result compared to the control experiment (6.94 cm). However,
the efficacy of botanicals increased with concentration and also significantly correlated with time
and reduction in mycelia extension of the pathogen. More so, variability in the antifungicidal
potentials of the botanicals on Macrophomina phaseolina ranges from 15.93% to 34.06%
according to Eigen proportions. The treatment combinations of; Ficus asperifolia, Mormordica
charantia and Anacardium occidentals at 75mg ml-1
concentration level produced the most
inhibitory effect against Macrophomina phaseolina in vitro. However, the untreated plates did not
show inhibitory effect on the mycelial growth of the pathogen. Therefore, combined treatments of
botanicals could be a potential source in the practise of plant disease control.
Keywords: Macrophomina phaseolina - Mycelial growth - Correlation - Eigen proportion
[Cite as: Akanmu AO, Olawuyi OJ, Bello OB, Akinbode OA, Aroge T, Oyewole B & Odebode AC (2015)
Genotypic variations in the inhibitory potentials of four combined botanicals on mycelia growth of
Macrophomina phaseolina of cowpea [Vigna unguiculata (L) Walp.]. Tropical Plant Research 2(3): 257–263]
INTRODUCTION
Macrophomina phaseolina (Tassi) Goid. belongs to the family Botryosphaeriaceae. It is a highly polyphagus
necrotrophic fungal pathogen that infects more than 500 plant hosts (Wyllie 1988). The plants invaded by this
pathogen are; major food crops, pulse, fiber and oil crops (Su et al. 2001, Mayek-Pérez et al. 2001, Dinakaran &
Mohammed 2001, Aly et al. 2007, Khan 2007). Macrophomina phaseolina have a wide geographical
distribution, and is especially found in tropical and subtropical countries with arid to semi-arid climates in;
Africa, Asia, Europe, and North and South America (Wrather et al. 2001). Diseases caused by Macrophomina
phaseolina include ashy seedling blight, seedling damping-off, charcoal rot, stem rot, and root rot leads to
significant yield loss (Emechebe & Lagoke 2002, Wellington et al. 2011). Incidences of these diseases are
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www.tropicalplantresearch.com 258
favoured at low soil moisture (Sandhu et al. 1999, Islam et al. 2012) and high temperatures (30–35°C). The
comparatively high capacity of Cowpea to withstand drought stress, poor soil conditions and high temperatures
made the crop very valuable especially in arid and Sub-Saharan regions of West Africa where cowpea is a crop
of major economic importance for resource poor farmers (Sanginga et al. 2003).
The persistence of sclerotia of Macrophomina phaseolina in the soil and plant debris (Short et al. 1980)
coupled with its wide host range has resulted in difficulty of the disease control especially on Cowpea where the
pathogen constitutes a major yield-suppressing factor even when under drought stress (Wyllie 1993). The
ineffective chemical control approach against Mormordica phaseolina necessitates a biological control measure
(Mark & Norman 2007). However, disease caused by fungi pathogens and their control with botanicals has been
found to be environmental friendly and effective against the targeted pathogen (Odebode 2006, Akanmu et al.
2013a, Abiala et al. 2013). This study investigates the efficacy of the interactive treatments of the extracts of
Ficus asperifolia, Mormordica charantia, Anacardium occidentals and Psidium guajava alone and in treatment
combinations against the pathogenic Macrophomina phaseolina of Cowpea.
MATERIALS AND METHODS
Source of pathogen
The pathogenic strain of Macrophomina phaseolina isolated from Cowpea was obtained from the plant
pathology laboratory, International Institute of Tropical Agriculture (IITA) Ibadan, Oyo state, Nigeria.
Source of plant extracts
Fresh leaves of Ficus asperifolia, Mormordica charantia, Anacardium occidentales and Psidium guajava
were obtained from Botanical garden and authenticated at the herbarium laboratory, both of the Department of
Botany, University of Ibadan, Ibadan, Nigeria.
Preparation of plant extracts
The fresh leaves were washed in clean water to be freed from possible dirt and debris. The cleaned leaves
were soaked in 5% Sodium hypochlorite solution for 10 minutes and rinsed in three exchanges of distilled water
to neutralize the effect of Sodium hypochlorite. The leaves were then air dried for two week weeks after which
they were blended separately into powdery form. The ethanolic extraction was carried out by weighing 2.5 g,
5.0 g and 7.5 g of each powdered extracts separately into 100ml of 75% ethanol, to achieve 25 mg ml-1
, 50 mg
ml-1
and 75 mg ml-1
concentration levels respectively.
In vitro control of Macrophomina phaseolina with plant extracts
The Macrophomina phaseolina culture obtained was asceptically subcultured on Potato Dextrose Agar
(PDA) and incubated for 7 days. The biocontrol experiment using plant extracts was carried out by adding 1ml
of the extracts to 9 ml of PDA poured on each plate. This was observed on each concentration level of the four
plant extracts used in this study. For the interactive treatment which involves the combination of two extracts,
0.5 ml of each extracts were added to 9ml of PDA poured per plate. The interaction of three extracts were
prepared by adding 0.33 ml of each extract to 9 ml of PDA while for the different four extracts used, 0.25 ml of
each were added to 9 ml of PDA. The extracts amended molten PDA was initially swirled gently for
homogenization before the plates were poured and allowed to solidify. Using a 5 mm cork borer, the mycelia
growth from the advancing edge of the 7 day old pure culture of Macrophomina phaseolina were picked and
inoculated at the centre of extracts treated PDA plates. The control experiment consisted of the treatments of 1
ml 75% ethanol with 9 ml of PDA. The plates were then incubated for 8 days, during which data on the radial
growth of the pathogen in each plate were taken at the 4th
day, 6th
day and 8th
day of the experiment.
Percentage inhibition of mycelial growth by the leaf extracts was calculated using the formula:
Where: %MGI = % Inhibition of mycelial growth
DC = diameter of control
DT = diameter of test
Data analysis
Data obtained from the radial growths were subjected to the Analysis of Variance (ANOVA) using System
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Analysis Software package (SAS) 9.1 2009 version, while means were separated by Duncan Multiple Range
Test (DMRT).
RESULTS
Highly significant (p<0.0) result was obtained on the radial growths of Macrophomina phaseolina by
activities of the plant extracts, with respect to period (day) of the study, also, in the interactions involving;
concentration x day, extracts x concentration, extracts x day, extracts x replicates, extracts x concentration x
day, also, extracts x replicate x concentration. While significant (p<0.05) effects were produced in the
interactions of concentration x day and extract x replicates x day (Table 1).
Table 1. Interactive effect of extract, replicate, concentration and day of data collection on the
mycelia growth of Macrophomina phaseolina.
Source df Radial Growth
Concentration 2 1.46ns
Day 2 1624.83**
Extracts 15 33.01**
Replicates 3 0.13ns
Concentration x Day 4 1.32*
Extracts x Concentration 30 8.10**
Replicates x Concentration 6 0.19ns
Extracts x Day 30 3.61**
Replicates x Day 6 0.03ns
Extracts x Replicates 45 1.33**
Extracts x Concentration x Day 60 1.23**
Replicate x Concentration x Day 12 0.51ns
Extract x Replicate x Concentration 90 1.18**
Extract x Replicate x Day 90 0.82*
Error 180 101.21
Corrected total 575 4527.88
Note: ** = Highly significant (p<0.01), * = Significant (p<0.05), ns = not significant.
Considering the sole treatment of the four botanicals tested, Mormordica charantia (4.34 cm) had the
highest mean inhibitory effect of 4.34 cm on the mycelial growths of Macrophomina phasolina, while F.
asperifolia (5.69 cm), Anacardium occidentals (5.59 cm) and Psidium guajava (5.55 cm) which showed slight
inhibitory effect on the pathogen, were not significantly (p>0.05) different from the control (5.69 cm). A more
significant (p<0.05) control of the pathogen was achieved by the effects of the combined treatments of the four
extracts, with the extracts of Mormordica charantia and Psidium guajava (3.29 cm) as well as Ficus asperifolia,
Mormordica charantia and Anacardium occidentales (3.11 cm) on Macrophomina phaseolina (Table 2).
Table 2. Inhibitory effect of interaction of extracts on the mycelia growth of Macrophomina phaseolina.
Extracts Radial growth (cm)
Anacardium occidentals 5.59b
Psidium guajava 5.55b
Ficus asperifolia and Mormordica charantia 4.79c
Ficus asperifolia and Anacardium occidentals 4.61dc
Ficus asperifolia and Psidium guajava 4.63dc
Mormordica charantia and Anacardium occidentals 3.84gh
Mormordica charantia and Psidium guajava 3.29ij
Anacardium occidentals and Psidium guajava 4.76c
Ficus asperifolia, Mormordica charantia and Anacardium occidentals 3.11j
Ficus asperifolia, Mormordica charantia and Psidium guajava 4.36de
Mormordica charantia, Anacardium occidentals and Psidium guajava 4.16efg
Ficus asperifolia, Anacardium occidentals and Psidium guajava 3.95fg
Ficus asperifolia, Mormordica charantia, Anacardium occidentals and Psidium guajava 3.53hi
Control 6.94a
Note: The significant difference (p<0.05) is indicated by different letters along each column.
The growth inhibition at 75 mg ml-1
concentration produced significant (p<0.05) effect, followed by 25 mg
ml-1
, while the concentration of 50 mg ml-1
had the least mycelia inhibition. The radial growth of the pathogen
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increases with time in which the most significant growth was recorded on the 8th
day of data collection (7.32
cm), followed by the 6th
day (4.63 cm) while the least was recorded on the fourth day (Table 3).
Table 3. Mean performance of botanical concentration, days of observation and replicated treatments
on the mycelia growth of Macrophomina phaseolina.
Observed characters Variables Radial growth (cm)
Extract concentration (mg ml-1
) 25 4.57a
50 4.49ab
75 4.40b
Days 4 1.51c
6 4.63b
8 7.32a
Replicates 1 4.50a
2 4.51a
3 4.48a
4 4.45a
Note: The significant difference (p<0.05) is indicated by different letters along each column.
The day of observation is positive and significantly (p<0.05) correlated with concentration of the extract and
mycelia growth with r=0.15 and 0.85. There was negative association between the extracts and the mycelia
growth with r=0.22 while non-significant and negative association occurs between the radial growth and
replicate as well as concentration, no association exists between extracts and days of observation, concentration
and the replicates (Table 4).
Table 4. Effect of Correlation of the extracts; extract concentration, days of observation and
replicates on the radial mycelia growth of Macrophomina phaseolina.
Correlation Extracts Replicates Concentration Day
Replicates 0.00ns
Concentration 0.00ns
0.00ns
Day 0.00ns
0.00ns
0.15*
Radial growth -0.22* -0.01ns
-0.01ns
0.85**
Note: ** = Highly significant (p<0.01), * = Significant (p<0.05), ns = not significant.
The contribution of principal component analysis (PCA) in tables 5 showed variations in the Eigen values
and proportion of the mycelial growth. Prin 1 accounted for the highest variation with the highest Eigen vector
for extract, concentration and day of experimental observation at proportion of 34.06%. The first and fourth
PCA showed positive and more relatedness for the extracts treatments. The extract concentration of the second
PCA had the highest Eigen value which accounted for 25.01% of total variation while the third PCA had the
highest vector for the replicates (Table 5).
Table 5. Contribution of principal component analysis (PCA) to the variation in the radial growth
of Macrophomina phaseolina.
Radial growth Prin1 Prin2 Prin3 Prin4
Extracts 0.706 -0.067 -0.011 0.705
Replicates 0.022 0.389 0.920 0.003
Concentration 0.035 0.919 -0.390 0.047
Day 0.707 0.010 0.002 -0.707
Eigen values 1.360 1.000 1.000 0.637
Proportion (%) 34.06 25.01 25.00 15.930
DISCUSSION AND CONCLUSION
Research has demonstrated that biological control is a potentially feasible alternative to the use of pesticides
(Jacobsen et al. 2004, Adandonon et al. 2006, Sobowale et al. 2008, Olawuyi et al. 2011, Akanmu et al. 2012,
2013a, Olawuyi et al. 2013). The combined use of biocontrol agents in the control of some important pathogens
affecting crops of economic importance is a new trend in plant disease control experiments. The integrated
approach of biocontrol research adopted in this research using botanicals of; Ficus asperifolia, Mormordica
charantia, Anacardium occidentals and Psidium guajava at different concentrations levels in sole and in
combinations towards the control of the mycelial growth of Macrophomina phaseolina of cowpea is in line with
the observations earlier made (Odebode 2006, Adandonon et al. 2006).
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Macrophomina phaseolina had been reported as the cause of charcoal rot disease which is a major biotic
factor that limits cowpea productivity worldwide (Ma et al. 2010, Zveibil et al. 2012, Arshad et al. 2012). This
was demonstrated in the reaction of cowpea varieties to infection of Macrophomina phaseolina obtained from
six different leguminous plants in Nigeria, as was earlier investigated by Amusa et al. (2007). The possible
control of this pathogen using botanicals as investigated in this research showed the efficacy of all the extracts
tested when compared to the results obtained in the control experiment. The effectiveness of the botanicals
against Macrophomina phaseolina could be attributed to their antifungal properties as was also discovered by
Arshad et al. (2012) who reported the efficacy of the antifungal properties of different parts of Sorghum
halepense Pers. in the control of Macrophomina phaseolina of cowpea.
The efficacy of the extract of Mormordica charantia which showed the most inhibitoriest potential on the
growth of Macrophomina phaseolina had earlier been reported in the treatment of human and plant diseases
(Virdi et al. 2003, Burger et al. 2010, Jonathan et al. 2012). However, the most significant mycelial inhibition of
Macrophomina phaseolina which was recorded in the combination of the extracts of; Ficus asperifolia,
Mormordica charantia and Anacardium occidentals, compared to the control experiment conformed to the
findings of Akhter et al. (2006) who observed 91% to 100% inhibition of conidial germination of Bipolaris
sorokiniana by plant extracts amended with cow dung and cow urine. The efficacy of combined treatment of
plant extracts was also affirmed in the combined treatments of botanicals and antibiotics against some emerging
drug-resistance microorganisms investigated by Rakholiva & Chanda (2012).
The significant result in interactions of the biological agents with the pathogen is in accordance with
findings of Sobowale et al. (2009), Akanmu et al. (2013b), Olawuyi et al. (2011, 2013). More so, the efficacy of
botanicals increases with concentration while it was also significantly correlated with time and the reduction in
the mycelia extension of the pathogen as similar reported by Akinbode (2010).
The significant correlation of the days of observation with extracts concentration and mycelia growth is an
indication of the positive contribution of the extract and their concentration in inhibition of Macrophomina
phaseolina as similarly observed by Akanmu et al. (2013a) and Olawuyi et al. (2014). The contribution of the
principal component analysis in this study is an indication of variability in the inhibitory potential of the
botanical extracts on the mycelia growth of the pathogen in accordance with the findings of Olowe et al. (2013).
The mycelial growths of Macrophomina phaseolina showed variability which ranges from 15.93% to 34.06%
due to the interactive treatments of botanicals at varying concentration over a period of time according to Eigen
proportion. The variations in the extract performances could be attributed to differences in their anti-fungicidal
properties, as similarly confirmed by Burger et al. (2010), Olawuyi et al. (2010) and Fapounda et al. (2011).
This suggests that there are variations in bioactive of antifungal compounds with varying characteristics and
potentials in their modes of action. However, the effectiveness of botanicals in field condition could
subsequently affirm the findings of this study to prove further their potentials in plant disease control.
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