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Termnology
1- Hypochromia :- abnormality of staining of RBC , the
cells are poorly filled with hemoglobin , and the normal
pale central zone may expand until only a faint rim of
pink stain Remains around the periphery of the cells as
in iron deficiency anemia.
2- Hyperchromia :- Abnormality of staining of RBC , the
cell Are well area disappear.
3-polychromesia :- The RBC stain with more than one color.
R .B . Cs inclusion bodies:
In the R.B. Cs in the form of bodies of different shapes
and sizes, origin, number and staining.
Those are:
1. Howell – Jolly bodies:- are about 1 nm in diameter , are
usually single and are centrically placed round purple
staining They are derived from chromosomes during
abnormal mitosis They occur singly in hemolytic anemia
and following splenectomy Multiple Howell-Jolly bodies
in one red blood cell occur in megaloblastic anemia and
in other forms of nuclear maturation defect.
2- Basophilic stippling :- these are many coarse or fine
purple – staining granules, result from aggregation of
ribosomes and are found in lead poisoning , anemias
with impaired hemoglobin synthesis, alcoholism, and
megaloblastic anemia.
3-Heinz bodies:- They are highly retractile , usually
peripheral may be multiple , vary from 1 to 2nm
upwards. They consist of denatured hemoglobin and are
caused by the action of various oxidant drugs and
chemicals, also produced in cells with defective enzyme
systems such as glucose-6-phosphate dehydrogenase
deficiency , or if unstable Hb. Are present, and they
appear after splenectomy . They are only demonstrated
by super avital staining
4-siderocyte granules ( Pappenheimer bodies ):- are
iron containing granules commonly appear in pairs or
multiples of two their precise cause is unknown but they
seem to be iron which is taken into the cell but which
has not been incorporated into Hb . They stain nearly
black , they are found in greatest number in patients
with splenectomy.
5- Cabot ring bodies :- very rarely seen , fine rings of
deeply staining material – probably denatured proteins
– and occur in the red cells of patient with sever anemias
and lead poisonhng.
Rouleaux formation :- arrangement of red blood cells
in rolls or stacks. This may be due to an artifact as a
result of not preparing the blood soon after placing the
blood on the slide, it also found in multiple myeloma
and macroglobulinemia.

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Abnormalities in color of RBCs

  • 1.
  • 2. Termnology 1- Hypochromia :- abnormality of staining of RBC , the cells are poorly filled with hemoglobin , and the normal pale central zone may expand until only a faint rim of pink stain Remains around the periphery of the cells as in iron deficiency anemia.
  • 3. 2- Hyperchromia :- Abnormality of staining of RBC , the cell Are well area disappear.
  • 4. 3-polychromesia :- The RBC stain with more than one color.
  • 5. R .B . Cs inclusion bodies: In the R.B. Cs in the form of bodies of different shapes and sizes, origin, number and staining. Those are:
  • 6. 1. Howell – Jolly bodies:- are about 1 nm in diameter , are usually single and are centrically placed round purple staining They are derived from chromosomes during abnormal mitosis They occur singly in hemolytic anemia and following splenectomy Multiple Howell-Jolly bodies in one red blood cell occur in megaloblastic anemia and in other forms of nuclear maturation defect.
  • 7.
  • 8. 2- Basophilic stippling :- these are many coarse or fine purple – staining granules, result from aggregation of ribosomes and are found in lead poisoning , anemias with impaired hemoglobin synthesis, alcoholism, and megaloblastic anemia.
  • 9. 3-Heinz bodies:- They are highly retractile , usually peripheral may be multiple , vary from 1 to 2nm upwards. They consist of denatured hemoglobin and are caused by the action of various oxidant drugs and chemicals, also produced in cells with defective enzyme systems such as glucose-6-phosphate dehydrogenase deficiency , or if unstable Hb. Are present, and they appear after splenectomy . They are only demonstrated by super avital staining
  • 10.
  • 11. 4-siderocyte granules ( Pappenheimer bodies ):- are iron containing granules commonly appear in pairs or multiples of two their precise cause is unknown but they seem to be iron which is taken into the cell but which has not been incorporated into Hb . They stain nearly black , they are found in greatest number in patients with splenectomy.
  • 12. 5- Cabot ring bodies :- very rarely seen , fine rings of deeply staining material – probably denatured proteins – and occur in the red cells of patient with sever anemias and lead poisonhng.
  • 13. Rouleaux formation :- arrangement of red blood cells in rolls or stacks. This may be due to an artifact as a result of not preparing the blood soon after placing the blood on the slide, it also found in multiple myeloma and macroglobulinemia.