This document provides an overview of biocomposting. It describes the three phases of composting (mesophilic, thermophilic, and curing), the key organisms involved (bacteria, actinomycetes, fungi, earthworms), materials used, and common composting methods like the Indore and Bangalore approaches. The benefits of composting are highlighted as improving soil quality by adding nutrients, improving soil structure, and enabling plant growth. In conclusion, composting is presented as an economically and environmentally sound waste management process.
Composting is nature's process of recycling decomposed organic materials into a rich soil known as compost. Anything that was once living will decompose
Composting is nature's process of recycling decomposed organic materials into a rich soil known as compost. Anything that was once living will decompose
A pesticide can be defined as any substance or mixture of substances intended for preventing, destroying, repelling, or mitigating any pest.
Pesticides like insecticides, herbicides, fungicides, and various other substances are used to control or inhibit plant diseases and insect pests.
The positive aspect of application of pesticides renders enhanced crop/food productivity and drastic reduction of vector-borne diseases.
However excessive use of these chemicals leads to the microbial imbalance, environmental pollution and health hazards.
Due to these problems, development of technologies that guarantee their elimination in a safe, efficient and economical way is important.
This ppt contains all types of Microbial Bioremediation methods . Everyone can understand clearly . Explaining with neat pictures and animation . Useful for presentation about Microbes in bioremediation . At last it contains a small animated video which helps to get clear view .
Vermitechnology means rearing of earthworms. earthworm is friend of farmer. earthworm is doing a great job and also produced a good organic manure is called vermicompost. vermicompost is a biofertilzer. which is enhancing soil qualities. This is explained earthworm biology, importance and preparation of vermicompost, vermiwash, panchgavya and their importance.
Introduction
Type of pesticides
Advantage & disadvantages of pesticides
Degradation of pesticide
Microbial degradation of pesticides
Mode of microbial metabolism of pesticides
Strategies for biodegradation
Approaches for biodegradation of pesticide
Chemical reaction leading biodegradation of pesticide
Metabolism of pesticides by MO
Metabolism of DDT
In his PPT you will come to know about the TREATMENT OF SOLID WASTE, ITS MANAGEMENT and MICROORGANISMS INVOLVED IN THE TREATMENT OF SOLID WASTE. do like, share and follow me to get more such PPT to be uploaded.
A pesticide can be defined as any substance or mixture of substances intended for preventing, destroying, repelling, or mitigating any pest.
Pesticides like insecticides, herbicides, fungicides, and various other substances are used to control or inhibit plant diseases and insect pests.
The positive aspect of application of pesticides renders enhanced crop/food productivity and drastic reduction of vector-borne diseases.
However excessive use of these chemicals leads to the microbial imbalance, environmental pollution and health hazards.
Due to these problems, development of technologies that guarantee their elimination in a safe, efficient and economical way is important.
This ppt contains all types of Microbial Bioremediation methods . Everyone can understand clearly . Explaining with neat pictures and animation . Useful for presentation about Microbes in bioremediation . At last it contains a small animated video which helps to get clear view .
Vermitechnology means rearing of earthworms. earthworm is friend of farmer. earthworm is doing a great job and also produced a good organic manure is called vermicompost. vermicompost is a biofertilzer. which is enhancing soil qualities. This is explained earthworm biology, importance and preparation of vermicompost, vermiwash, panchgavya and their importance.
Introduction
Type of pesticides
Advantage & disadvantages of pesticides
Degradation of pesticide
Microbial degradation of pesticides
Mode of microbial metabolism of pesticides
Strategies for biodegradation
Approaches for biodegradation of pesticide
Chemical reaction leading biodegradation of pesticide
Metabolism of pesticides by MO
Metabolism of DDT
In his PPT you will come to know about the TREATMENT OF SOLID WASTE, ITS MANAGEMENT and MICROORGANISMS INVOLVED IN THE TREATMENT OF SOLID WASTE. do like, share and follow me to get more such PPT to be uploaded.
ORGANIC FARMING : COMMON ORGANIC MANURES SMGsajigeorge64
A brief account of common organic manures - Bone meal, cow dung, poultry wastes, oil cakes, organic mixtures, compost and vermicompost, vermiwash , advantages and disadvantages of composting & vermicomposting.
Compost and Lanfills (process and designs)Aneela Rafiq
compost and Landfills are most important for balance soil. it helps in maintain the soil to has enough nutrients to grow health plants without destroying natural environment and living things in surroundings.
you will find sufficient material about composts and landfills in this presentation.
In recent years, it is no doubt that in India, where on one side pollution is increasing day by day due to accumulation of organic waste and on the other side there is a great shortage of organic manure.
It has been estimated that India, as a whole, generates as much as 25 million tonnes of urban solid waste of diverse composition per year. Solid waste comprises of both organic and inorganic matter.
Under the present condition of environmental degradation, vermicomposting technology is the best way to meet all the requirements of the society. This is a process of recycling trash/agricultural wastes in an efficient and eco-friendly manner in order to produce quality compost.
Organic wastes can be broken down and fragmented rapidly by earthworms, resulting in a stable non-toxic material with good structure, which has a potentially high economic value and also acts as a soil conditioner for plant growth.
It is a type of composting in which worms eat and metabolize organic matter that comprises to a better end product known as Vermicast (commonly called as BLACK GOLD) which has a stuff of nutrients that can be directly incorporated into the soil to help with plant fertilization, soil enrichment and soil stability.From a social point of view, organic fertilizers will:
Improve the social status of the individuals and the community.
Create motivation for people to live in the countryside by providing job
opportunities and business plans.
From a hygienic point of view, organic fertilizers will:
Produce chemical-free crops which will improve people's health.
Reduce the danger of lung diseases and other diseases resulting from burning the organic wastes in the field.EPIGEIC EARTHWORMS:
Earthworms of this group cannot make burrows in the soil. They can only move through crevices of the surface. They feed exclusively on decomposing organic wastes.
ENDOGEIC EARTHWORMS:
They are subsoil dwellers. Secretions of body wall of earthworms cement and smoothen the walls of the burrows and protect the wall from collapsing easily. They move below 30cm or more in the soil
ANECIC EARTHWORMS:
They are found in the soil, which is not frequently disturbed. They make very complicated burrows in the sol and they firmly pack their burrow walls with their castings. The Anecic earthworms like Epigeic earthworms are commonly found in temperate countries.Vermicompost is an excellent soil additive made up of digested compost. Worm castings are much higher in nutrients and microbial life and therefore, are considered as a higher value product. Worm castings contain up to 5 times the plant available nutrients. It not only adds microbial organisms and nutrients that have long lasting residual effects, it also modulates structure to the existing soil, increases water retention capacity. Vermicompost contains an average of 1.5% - 2.2% N, 1.8% - 2.2% P and 1.0% - 1.5% K. The organic carbon is ranging from 9.15 to 17.98 and contains micronutrients Nitrogen, phosphorus, Potassium..
Compost is a mixture of ingredients used to fertilize and improve the soil. It is commonly prepared by decomposing plant and food waste and recycling organic materials. The resulting mixture is rich in plant nutrients and beneficial organisms, such as worms and fungal mycelium. Compost improves soil fertility in gardens, landscaping, horticulture, urban agriculture, and organic farming. The benefits of compost include providing nutrients to crops as fertilizer, acting as a soil conditioner, increasing the humus or humic acid contents of the soil, and introducing beneficial colonies of microbes that help to suppress pathogens in the soil. It also reduces expenses on commercial chemical fertilizers for recreational gardeners and commercial farmers alike.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
This pdf is about the Schizophrenia.
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Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
1. A SELF STUDY REPORT ON TOPIC
BIOCOMPOSTING
• Presented by:- Parveen kumar
• Msc. BOTANY (3rd SEM)
• ROLL no. 170000302010
Composting
2. CONTENTS
• Introduction
• Benefits of composting
• Mechanism of composting
• Phases of composting
• Organisms involved in composting
1. Bacteria
2. Actinomycetes
3. Fungi
4. Earthworms -:Vermicomposting
• Material required for composting
• Methods for composting
• Result and discussion
• Conclusion
• References
3. INTRODUCTION
• Composting is a waste managing biological process
• Microoganisms such as bacteria , fungi , and actinomycetes decomposes
organic matter into co2 ,water , heat and humus
• Compost -:compost is organic matter that has been decomposed and
recycled as fertilizer & soil amendent
• Composting can be either aerobic or anaerobic
• Aerobic composting requires oxygen while anerobic not
• Composting organisms requires 4 ingredients to work effectively -:1)carbon
2)nitrogen 3)oxygen 4)water
4. BENEFITS OF COMPOSTING
• Improves soil quality by adding nutrients such as nitrogen , phosphorus and
magnesium etc. –Soil conditioner
• Improve water holding capacity of soil
• Enhances structure and texture of soil
• Enables the soil to retain nutrients , moisture , & air – for plant growth
• Micronutrients such as Mn , Cu , Fe , & Zn also found in compost
Parameters in Composting
Carbon: nitrogen ratio 30:1
Ideal moisture 50 – 60%
Ph 6 – 8
Temperature 55 – 75°C (thermophile range)
Oxygen availability 5 -15
5. MECHANISMS OF COMPOSTING
• Composting is biochemical process
• Aerobic and anerobic microorganisms decomposes organic matter into valuable
manure –compost
Mesophilic state [25 – 30°c] promotes mesophilic microbes
Organic matter organic matter compost
temp 55 - 60°c [ C:N ratio 25:1]
[Thermophilic state - destroy pathogens]
Finally compost is made and utilize as fertilizer
Rich in nutrients –N , P , & C – for plants growth
6.
7. PHASES OF COMPOSTING
There are 3 phases for composting
1.Mesophilic phase
Mesophilic microorganisms rapidly breakdown the soluble & readily
degradable compounds
Temp range 10 – 40°c is considereed
2.Thermophilic phase
Temp raise & enters to thermophilic range
Temp range 40 – 70°c
High temp accelerate the breakdown of [proteins , fats ,starch ,cellulase &
hemicellulase ]
3.Curing or maturation phase
Temp cool down & mesophilic microbes once again
Almost all organic substrates are degraded
Final stage of aerobic composting
9. ORGANISMS INVOLVED IN COMPOSATING
BACTERIA
Smallest and most numerous
biological component
Responsible –decomposition & heat
generation in compost
Process start with mesophile
[predominant]
Bacteria –bacillus genus are
dominates mesophilic phase
E.g. of bactera – Bacillus brevis & B.
subtilis
10. Continue…..
Actinomycetes
Higher form filamentous bacteria &
resemble with fungi
Complex organic compounds – cellulose ,
lignin , chitin & proteins
Enzymes –chemically breakdown tough
debris [ woody stems , bark or news
paper]
Release –carbon , nitrogen & ammonia
[earthy smell of compost]
Some species appear in thermophilic
phase and others in curing phase
Decompose more resistant compounds
E.g. Actinobifida chromogena,
Microbispora bispora
11. Continue…..
Fungi
Responsible for decomposition of complex
plant polymers
complex polymers –polyromantic compounds
& plastics
Breaks tough debris & enables bacteria to
continue the process
Extremely dry , acidic , or low in nitrogen
dealt by fungi
Live on outer surface of compost as gray
colonies
Numerous in both phases –mesophilic &
thermophilic
E.g. Aspergillus fumigatus , Humicola grisea
13. Earthworms
Most important and large physical decomposers
Ingest organic matter & digest it with the help of tiny
stones [gizzards]
Leave dark & fertile casting
Each day casting =worms weight
Castings are rich in plant nutrients – N , Ca , Mg , & P
14. Suitable species of worms
Eisenia fetida Eisenia hortensis
Lumbricus
rubellus Eudrilus eugeniae Perionyx excavatus
15. Vermicomposting
Using worms to recycle food scraps & other organic material into
valuable soil amendment –vermicompost
Mesophilic process -earthworms & microbes active @ 10 -
32°C
Consume various organic waste & reduce the volume by
40-60%
Containing water-soluble nutrients
Vermicompost is an excellent, nutrient-rich organic fertilizer and
soil conditioner
16. MATERIAL REQUIRED FOR COMPOSTING
1.Weeds 1. Cow dung 1. Night soil
2.Stubbles bhusa 2. Buffalo dung 2. Street refuge
3.Crop residues 3. Poultry dung 3. Municipal refuse
4.Remments of fodder
Farm
refuses
Animal
dung
Town
refuse
17. Methods for composting
Indore method
Size of the pit :- Breadth 6 - 8 feet
Depth 2 - 3 feet
Length 10 feet or more as per requirement
Raw material :- mix plant residues , weeds , sugarcane leaves , grass
,wood ashes , bran etc. animal dung , water , urine soaked mud
Filling the composting pits :-spread dry waste with cattle
dung & soil in ratio of 4:2:1 upto 2 inch
Sprinkle water over material
Filled the pit with raw matter upto 1 feet above the ground
level
18. continue
• Turning –material is turned 3-4 times for proper aeration &
moisture
• First tuning :- 10-15 days after filling the pits
• Second turning :- 15 days after first turning
• Third turning :- after 2 months of second turning
Turning
19. Continue……
Bangalore method :
• Worked out by L.N. Acharya at Indian institute of science , Bangalore
• This method saves Labour cost
• No need of turning & regular sprinkling of water
Method of filling the composting pits :-
Spread moist farm refuse @bottom of the pit upto 1 inch
Then , spread cattle dung & urinated mud followed by 1 or 2 inch soil layer
Finally heap is made up up to 1.5 – 2 feet above ground level
Heap is covered with thick mud
20. After 8-9 months all material decomposes and
compost becomes ready for the application.
Bangalore
method
21. • Microorganisms degrades organic waste into nutrients
• Produce organic fertilizer – manure
• Reduces reliance on chemical pesticides & fertilizers
• Improve plant growth & water holding capacity of soil -conserve water
• Bacteria excreting N , P , Mg in soil & utilize by plants
• Compost used as soil amendment for agriculture
• Soil building & erosion control
• Good source of minerals & microorganisms
• Finally decomposed organic matter – HUMUS
• Reduces soil temp fluctuations
• Inc. water & nutrients holding capacity of soil
• Serve as nitrogen reservoir for plant
• Maintain C:N ration in soil – 30:1
22. conclusion
Converts raw organic matter into humus
Maintain high & healthy soil life level
Complex organic compounds breaks into simpler forms
Composting –best fitted waste managing option
Economically & environmentally sound method that avoids all
pollution concerns
23. References
Trautmann , Nancy , “CORNELL Composting –
Compost Microorganisms” CORNELL
Composting – Compost Microorganisms.
Cornell Waste Management Institute , Web. 13
Dec 2015
“Microbes in the compost pile.” Microbes in the
compost pile. Mansfield C. T. Web. 13 Dec 2015
David. R. Hershey , Sir Albert Howard an The
Indoor Process , 1992