7. dr jared serem dv tech mgr - nutritional approach to reduce antibiotic resistance

1. © Diamond V, Inc. All rights reserved. This presentation is the confidential and proprietary property of Diamond V. Diamond V does not give its consent for its distribution or license the use of its content.
Reducing Antibiotic Resistance in Animal
Production
Jared K. Serem, Ph.D.
Animal Health and Food Safety Manager - Pigs and Poultry
Diamond V - IMEA
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 Use of antibiotic growth promoters (AGPs) in
feed started in 1940’s
 AGPs adoption improved animal performance by
20%
 Increased antibiotic resistance linked to the sub-
therapeutic doses in animal feed
Antibiotic Growth Promoters
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Industry In Rapid Change

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Changing Consumer Preferences…
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 Saccharomyces cerevisiae fermentation
metabolites potential solution
 Reduces prevalence, shedding, virulence and
antibiotic resistance in:
 Salmonella
 E. coli
 Clostridium
 Campylobacter
 Maintained production performance
Is Farming Without AGPs Sustainable?
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How Do Diamond V Products Work?
 The unique metabolites created through our
exclusive fermentation process improves
health and performance of animals.
 Mode of Action:
 Enhances immune function
 Gut microbial balance and activity
 Improves intestinal morphology

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 Commercial broiler field trials
 Broiler companies
 Two Dietary Treatments
 CON diet: standard company diet
 CON diet plus XPC at 1.25 kg/MT from day of age to market
 Ceca harvested from 50 birds/house after evisceration in
commercial processing plants
 Ceca were shipped on ice overnight to Iowa State University for
E. coli analysis
Diamond V XPC: Avian Pathogenic
E. coli prevalence and AMR in broilers
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E. coli Testing Procedures
Pick E. coli
colonies from
individual ceca
samples
Multiplex PCR:
APEC Prevalence
Micro-Dilution Antibiotic
Resistance Testing:
Ceftiofur, Enrofloxacin and
Florfenicol Resistance
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0.0
20.0
40.0
60.0
80.0
100.0
CON XPC
TestedE.coliColonies
ConfirmedasAPEC(%)
Treatment
74.7a
32.1b
Survey of E. coli Colonies: APEC Genotyping
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a,bP <0.0001
Broiler Field Samples
Miller et al., 2017

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Pick E. coli colonies from individual ceca
samples into media and grow overnight
Count the number of wells with growth
and determine the percent resistant
Transfer to media containing
the targeted antibiotic
E. coli plate
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Antibiotic Resistance Testing: (Micro-Dilution)

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0.0
20.0
40.0
60.0
80.0
100.0
Florfenicol Ceftiofur Enrofloxacin
ColoniesResistant
toanAntibiotic(%)
CON XPC
Survey of Broiler E. coli Colonies: Antibiotic
Resistance
90.7a
25.0b
68.0a
15.4b
56.3a
26.9b
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Miller et al., 2017
a,bP <0.0001
Broiler Field Samples

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Necrotic Enteritis Mortality (<28d)
Broomhead et al., 2014
a,bP < 0.055 vs. PC

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Necrotic Enteritis Body Weight Gain (0-21d)
Broomhead et al., 2014
a,bP = 0.07

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Necrotic Enteritis Feed Conversion (0-21d)
Broomhead et al., 2014
a,bP < 0.0001

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Ceca Campylobacter coli: Prevalence and Numbers
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90.8a
76.7b
0.0
20.0
40.0
60.0
80.0
100.0
CON XPC
Prevalence(%)
Smith et al., 2014
32,360a
3,096b
0
10,000
20,000
30,000
40,000
CON XPC
CFU/g
a,bP = 0.0026 a,bP = 0.023

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Ceca Campylobacter coli: Prevalence and Numbers
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90.8a
76.7b
0.0
20.0
40.0
60.0
80.0
100.0
CON XPC
Prevalence(%)
Smith et al., 2014
4.5a
3.5b
0.0
2.0
4.0
6.0
CON XPC
Log10CFU/g
a,bP = 0.0026 a,bP = 0.023

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 90 broiler chicks
45 CON
45 TRT
 Treatment Categories:
Organic acids
Probiotics
Yeast cell wall products
Essential oils
Prebiotics
 Day 11: Campylobacter inoculation (104)
 Day 14, 35, 42: Ceca sampled (15 birds each)
EU Campybro Project
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Guyard-Nicodeme et al., 2015
EU Campybro Project: Day 42 Results

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Fecal Swab Salmonella:
Prevalence and Numbers - Calves
P < 0.0001
58.0a
4.5b
0.0
20.0
40.0
60.0
80.0
Control NutriTek
CFU/g
Multi-Dairy Project; n = 814
P < 0.0001
26.8a
13.4b
0.0
10.0
20.0
30.0
40.0
Control NutriTek
Prevalence(%)

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0.63
0.65
0.64
0.65
0.55
0.57
0.59
0.61
0.63
0.65
0.67
0.69
Control AGP XPC XPC + CWP
kg/d
Average Daily Feed Intake
1.49f
1.47e 1.47e 1.47e
1.40
1.42
1.44
1.46
1.48
1.50
Control AGP XPC XPC + CWP
F/G
Feed Conversion
22.7a
23.5b
23.3b
23.6b
22.2
22.4
22.6
22.8
23.0
23.2
23.4
23.6
23.8
Control AGP XPC XPC + CWP
kg
Body Weight (d 35)
427c
448d
443d
450d
400
410
420
430
440
450
460
Control AGP XPC XPC + CWP
g/d
Average Daily Gain
P < 0.06
P < 0.05
P < 0.01
van der Peet-Schwering, et al. 2007. J. Anim. Sci. 85:3099–3109
Nursery Pig Performance: Wageningen

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 Diamond V Original XPC
 Reduces APEC prevalence and resistance to antibiotics
 Reduced mortalities, and maintains performance in
necrotic enteritis challenge
 Reduces Campylobacter pathogen load
 Reduced Salmonella incidence and load in calves
 Improved performance in nursery pigs comparable to
AGPs
Take home message
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Looking Ahead