MAB Training session

1. USING MARKERS FOR
PARENT SELECTION IN PEACH

2. MARKERS FOR PEACH BREEDING
1. PARENT CHARACTERIZATION AND SELECTION
• Relationships between parents
• Cross planning
o Genetic distances
o Heterozygosity
• Breeder’s rights enforcement
2. SEEDLING MARKER-ASSISTED SELECTION
3. MARKER-ASSISTED INTROGRESSION

3. 167
178
183
189
141
147
141
147
141
147
125
129
131
131
125
131125178 183
189
178
PEDIGREE VALIDATION
P 1
P 2
H-A
H-B

4. SELECTING PARENTS FOR A CROSS: GENETIC DISTANCE
Do my pedigree data fit?
The DENDROGRAM
Clusters group genetically
similar parents
Do I need more variability?
Do I want to have a fixed
background where only a few
traits segregate?

5. SELECTING PARENTS FOR A CROSS: HETEROZYGOSITY
More heterozygosity in the
parents

More segregation in the
progeny
0 20 30 40 50 6010
% Heterozygosity

6. MARKER (SSR) FINGERPRINTING FOR
BREEDER’S RIGHTS PROTECTION

7. Procedure for Cultivar Identification
for Breeder’s Rights Protection
Creation of a DataBase with the molecular profiles
Base de Datos
• With the Spanish breeder’s
association (GESLIVE)
• Samples collected from
official sources

8. Comparison between the fingerprints
of a problem samples and the DB
Database
Fingerprint coincides with Var. 7
(Calculate the probability of inding by chance another variety with same fingerprint)
Procedure for Cultivar Identification
for Breeder’s Rights Protection

9. M3
MARKER-ASSISTED SELECTION
0-5 cM
M2
LINKED MARKERS: Require validation in a progeny (phase
knowledge)
DIAGNOSTIC MARKERS: Presence of certain alleles predict
corresponding phenotypes
M1IDEALLY “DIAGNOSTC”
MARKERS. FOR LINKED
MARKERS WE HAVE TO
KNOW THE PHASE
MARKER-SELECTED GENE

10. ×
×
×
×
×

11. A WHOLE-GENOME VIEW
OF THE PARENTS OF
A BREEDING PROGRAM (9K PEACH CHIP)

12. Thank you