About Rutgers Molecular Imaging Center and the Aspect M2 The Rutgers Molecular Imaging Center received the Aspect M2 in the summer of 2012. With the versatility of the M2, a wide range of studies and over 85000 scans have been completed at the center. We have had conventional scans such as cancers, traumatic brain injuries, contrast agents, and other biologicals. The center has also reviewed items such as flow measurements of toothpaste, water filters in action, and pills breaking down during digestion. Please join us as we review examples of the various scans completed here at Rutgers University. The Rutgers Molecular Imaging Center received the Aspect M2 in 2012. Since the M2’s arrival, it has been one of the most utilized instruments at our center. As of May 1st, 2021, the instrument has seen over 6900 hours of use generating 450 projects/patient files comprising a total of over 85,000 scans. Since the M2 was installed, a combination of 60 departments (over 100 researchers) from multiple universities and pharmaceutical companies have had scans completed at Rutgers. At least ten users from five different departments have been trained over the years to independently run the MRI. This webinar will highlight some of the scans completed here at the center. One major focus of the center has been the brain and central nervous system. Some examples are the following: Longevity scans of Traumatic Brain Injury have been completed using the Mouse Brain Coil. For another TBI investigator project, leakage into the ventricles post-injury has been tracked with the use of positive contrast agents. Negative contrast agents have been utilized to track stem cells traveling from the lower vertebrae up the spine to points of injury or accumulating around damage in the brain. Changes to the brain following low levels of radiation exposure have been observed using the rat coil. Spontaneous paralysis was reviewed when phenotyping a new mouse strain. A wound healing model tracked the progress of recovery within a spinal injury model. A second important focus involves cancer models actively scanned at Rutgers. Multiple investigators track the progress of lung cancer and its metastasis. Another investigator tracked cancers in the liver through an interesting negative contrast technique. One Cancer Institute of NJ investigator tracked prostate growth in the same mice for up to two years. Yet another research group tracked an aggressive lymphoma on a weekly basis. Other interesting cancer scans that have been recorded are tumors in the bone, under the tongue, breast duct, uterus, just to name a few of the ones scanned at the center.

1. Low Field MRI and its
Applications
at Rutgers Molecular
Imaging Center Dr. Derek Adler
Manager At Rutgers
Molecular Imaging Center

2. Rutgers Molecular Imaging Center presents
Aspect M2 MRI
Director: Ed Yurkow, PhD
Manager: Derek Adler
July 2021

3. Overview
• Aspect M2 Installed September 2011- Formally tracking scans as of
July 2012
• From July 2012 to May 2021:
– 85,592 scans have been (86034 as of 7/9/2021)
– Over 6,000 hours of MRI use Between July 2012 and May 2021
– Data produced on the MRI has been used in over 40 publications from Rutgers
investigators.
– At least 2 Rutgers grants are associated with scans being conducted on the MRI
– To date over 10 students have completed their PhD. thesis projects utilizing the
MRI into their research.
– 450 projects/patient
– Since the M2 was installed, a combination of 60 departments (over 100
researchers)
– Most analysis done in Vivoquant (some Pmod, Matlab, or ImageJ )
– MRI has been used in tandem with our Optical, Ultrasound, PET, CT, and other
imaging units here at Rutgers
– Images are without contrast unless noted

4. Aspect M2 Hours Per Year
2011 MRI went online September 2011
2012 297.25*
2013 947.75
2014 881.25
2015 563.25
2016 595.75
2017 485.25
2018 761.00
2019 692.75
2020 388.75
Instrument Hours
*Tracking of Instrument time started July 2012

5. Known RU Publications using the M2
2013 1
2014 3
2015 7
2016 7
2017 6
2018 2
2019 5
2020 9
2021 1
Investigator Publications using the M2

6. Institutes, Overall Departments,
Universities, and Pharmaceuticals
Universities:
• Princeton
• NYU
• Stevens
• Delaware Valley
• Chicago
• NJIT
• Rowan
• Cornell (demo)
• Thomas Jefferson
(virtual demo)
• Cancer Institute
of New Jersey
• Robert Wood
Johnson
University
School of
Medicine
• University of
Medicine and
Dentistry
• New Jersey
Medical School
• Rutgers School
of Dentistry
• Rutgers School
of Pharmacy
• New Jersey
Center For
Biomaterials
• Chem-
Biochemistry
Engineering
• Food Science
• Entomology
• WM Keck Center
for Collaborative
Neuroscience
• Chemistry and
Chemical Biology
• Genetics
• Engineering
• Comparative
Medicine
Resources
• Public Health
Research Institute
Pharmaceuticals:
• BioTSI
• Johnson and Johnson
• Colgate
• Kashiv
• Alizee
• PTC Bio
• Visikol
• Others

7. MRI (Magnetic Resonance Imaging)
• 1 T MR M2™ platform (Aspect Imaging)
• Small footprint / No significant magnetic field
• Good for 2D & 3D quantitative image analysis
• Spatial resolution  100 µm
• Soft tissue and organs in vivo / ex vivo, in vitro

8. Examples of MRI scans completed at RU
• Assorted Mustard-Lung
scans, Fibrosis
• Flow Measurements
• Powered Cell Phone
Batteries
• Water Filters
• Toothpaste
• Assorted Cancer and
Metastasis Models
• Skin Grafts
• Bladder Stones
• Uterine Scars
• Tooth Extraction
Inflammation
• Novel Contrast Agents
• Paralysis Models
• Mouse
• Rat
• Zebra Fish
• Koi
• Lizard
• Hibernating
Bees/Other
insects
• Rabbit
Spines
• Quail
• Snail
• Vegetables
(for training)
• Pig (skin
graft)
• Goat Aorta
• Human
Placenta
• And more….. 
• Fetal Mouse and Brain
• NASA (Rat Brains)
• Sudden Death
• Phenotyping
• Wound Healing
• TBI: Traumatic Brain
Injury
• Contraceptive Foams
and Gels (In-vivo)
• Stems Cells with
Contrast Markers
• Cannula Functionality
• Enlarged Prostates
• Muscle and Body
Composition
• Chagas Disease

9. Examples of Brain and CNS Imaging

10. Traumatic Brain Injury (TBI) -Mouse
*FSE: No contrast

11. Axial Example of TBI Mouse scan
*FSE: No contrast

12. Example 2
Example 1
Examples of TBI Review over time -Mouse
Day 1 Day 7 Day 21
Day 1 Day 7 Day 21
*FSE: No contrast

13. Traumatic Brain Injury Model 1
Left: Brain and ventricles FSE (Blue)
Right: Green signal indicated contrast leakage GRE within Ventricle space
TBI and leaking ventricles -Mouse
*Leakage detected by subtracting a GRE-EXT
Before and After the use of Magnavist

14. Measuring the Volume of Intensity Change
(Green indicates contrast signal)
604 605 Flox Red Flox None
3000-Max Ventricle FSE Intensity overlay on GRE Images
TBI and leaking ventricles continued

15. Cell Tracking: SPION-labeled Stem Cells: Spine -Rat

16. Cell Tracking within the Brain -Mouse

17. Exposure to Low levels of Radiation over a two-year period. Same that Astronauts would
be exposed to in a trip to Mars.
Brain changes post radiation exposure -Rat

18. Ventricles at assorted levels of radiation exposure
R1 R2 R3 R4 R5
Gp1
Gp2
Gp3
Gp4
Gp5
Group R1 R2 R3 R4 R5 Ave SEM Stats
1 57.5 26.8 5.9 39.3 16.8 29.3 8.96 NA
2 39.6 66.5 60.4 48.0 29.5 48.8 6.73 ns
3 74.2 81.4 94.7 42.3 55.4 69.6 9.32 ns
4 148.0 174.5 54.3 175.5 42.5 119.0 29.29 **p=0.0032
5 102.5 48.7 27.6 78.2 64.3 16.44 ns
Ventricles - Total Volume (mm3)
0
20
40
60
80
100
120
140
160
1 2 3 4 5
Volume
(mm3)
Group
TP18-AZM-01: Ventricles - Total Volume
**
Group R1 R2 R3 R4 R5 Ave SEM Stats
1 2998.5 3113.7 2681.9 2817.6 2677.5 2857.8 86.71 NA
2 3087.5 2928.8 2899.5 2939.4 3114.2 2993.9 44.35 ns
3 3116.4 2898.5 2841.5 2844.8 3121.5 2964.5 63.85 ns
4 2964.1 2828.8 2848.9 3113.1 2762.7 2903.5 61.65 ns
5 3111.7 2774.8 3049.1 3189.8 3031.4 90.22 ns
Brain - Total Volume (mm3)

19. Example exhibited a lesion (benign tumor?) – marked by the crosshairs
Continued

20. G1R1 G1R2 G3R1 G4R1 G4R4
L R
Baseline MR Images (top) & 3D-PET Images (bottom)
Overlay was completed using similar Head positioning between scanners
Examples of MRI results and PET Glucose uptake in the same brains

21. Rat Brains: MRI & 18F-FDG-PET
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G4R1: 18F-FDG Uptake in Brain
Left
Right
Control Mass / Tumor T2 Hyper-intense Region
*Brain evaluation of Glucose uptake done in Pmod Brain Mapping

22. Paralysis Observed in a Mouse Model of Pancreatic Cancer-
Mouse
MRI: Signal Void in Mouse Spine CT: ROIs observed in Spine
*Impaired bladder emptying in paralyzed mouse
*
*

23. Wound healing above a spinal Injury -Mouse
Control Mouse with no
spinal injury.
Markers used to indicate
injury location
Mouse with a spinal injury.
Wound is above spinal
location. Blood flow
regulation to the wound site
was reduced/absent versus
the control
Markers used to indicate
injury location

24. Cancer Imaging

25. Tracking a lung tumors over time
Lung cancer or metastasis -Mouse
Week 1 Week 2 Week 3
Week 4 Week 5 Week 6
*FSE No contrast. Scan time was about 3 minutes a mouse with 50 mice a day

26. MRI and SWIR -Mouse

27. Negative Contrast Agents Enhance Detection of Metastasis -
Mouse
Pre-Dose
Liver
1.5 hr Post-Dose
Metastases

28. Prostate -Mouse
Animals scanned weekly
Then every other week
Then Monthly over 72 weeks
Final scans completed after 2 years
Enlarged/Cancer
Control

29. Lymph node:
Mice had cancer cells injected in their front arm. Starting on day three, primary tumors were then tracked and scanned weekly over
the next three weeks. After three weeks the primary tumor was removed. Scans then continued tracking metastasis that formed after
the removal of the primary tumor.
Week 3 Week 4 Week 5 Week 6 Week 7
Lymphoma model -Mouse

30. Pancreatic -Mouse
Other Cancer examples

31. Colon Cancer -Mouse
Tumors confirmed upon necropsy

32. Pituitary -Mouse

33. Breast Cancer, Breast Duct Model -Rat
Retired Breeders, Lowe Left Teat

34. • Melanoma Tumor Model -
Mouse
• Melanoma Tumor Model
• (Optical)

35. • On the Spine-Cause of Paralysis

36. • Bone -Mouse
• (Reviewed on micro-CT and
Optical)

37. Lung Cancer: Liver and other Metastasis
-Mouse

38. • Uterine (with contrast) -
Mouse
Heart (Metastasis)
-Mouse

39. Tongue/Oral cancer slices (Brain coil) -Mouse

40. Molecular Imaging Center (RUMIC)
Continued
M5 M8
M3
M6
M1 M2
M10
M4

41. Cystinuria (stone formation) -Mouse
Bladder and Kidney Stones
Thickening of the
bladder wall and
tumors

42. Lung tumor PhD Student Project with Matlab

43. Other interesting scans
Lung Example
(with IT contrast)

44. MRI: Evaluation of Pulmonary Edema Rat
Day 1 Day 14
Day 7
Day 3
Vesicant
(i.t., Day 0)
Vehicle
(i.t., Day 0)
• Vesicants
• LPS
• Influenza
• Asthma
MRI-FSE (T2)
Edema (2D)
Processed Image
with ROI (3D)
MRI-FSE (T2)
Control (2D)

45. Lung Fibrosis/Edema -Rat
FSE GRE

46. MRI Continuous Analysis -Rat
Murray et al. 2020. Ann. NY Acad. Sci.

47. MRI Image Review: Segmentation of Fibrotic Lungs
-Mouse

48. Fetal Review -Rat
*Above: Fetus scanned in Mouse
Brain Coil. FSE GRE combined

49. Fetal Review -Mouse

50. Chagas: Loss of Fat-Tissue around the heart: GRE
Top Control, Bottom infected with Chagas

51. Chagas Disease Heart Measurements

52. Acknowledgement of the Labs whose
images were used in this presentation
• Dr. Subramanian School of Dentistry
• Dr. Nagajyothi Public Health Inst.
• Dr. Reimer CMR
• Dr. Raskin Plant Biology
• Dr. Stapleton School of Pharmacy
• Dr. Kong School of Pharmacy
• Dr. Chen School of Pharmacy
• Dr. Sahota Genetics
• Dr. Minko School of Pharmacy
• Dr. Garbuzenko School of Pharmacy
• Dr. Gow School of Pharmacy
• Dr Santanum CINJ
• Dr. Paolo UMDNJ
• And those that I have missed.
• Dr. Adler RWJMS
• Dr. Contreras NJMS
• Dr. Kumar Biomedical Engineering
• Dr. Azzam NJMS
• Dr. Moghe Biomedical Engineering
• Dr. Hatefi School of Pharmacy
• Dr. Sophou Biomedical Engineering
• Dr. Laskin School of Pharmacy
• Dr. Sinko School of Pharmacy
• Dr Gao School of Pharmacy
• Dr. Prud'homme Princeton Chemical &
Biological Engineering
• Dr. Goldberg Rowan Molecular Biology

53. Special Thanks to the following for Direct
Contributions to this presentation:
• Dr. Yurkow Rutgers Molecular Imaging Center
• Dr. Wu Rutgers Molecular Imaging Center
• Dr. Shapley Biomedical Engineering
• Dr. Ganapathy Biomedical Engineering
• (Now) Dr. Murray School of Pharmacy
• (Now) Dr. Nomani School of Pharmacy

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