2. INTRODUCTION
2
Centrifugation: It is the process in which
centrifugal force is used as driving force
for Separation.
Centrifuge: The equipment used for
separation is known as centrifuge.
3. 3
INTRODUCTION
• Centrifugation is the process in which centrifugal force is
used as driving force for phase separation.
• The separation between the liquid and solid phases are on the
basis of the particle size and density difference
The equipment used for separation is known as centrifuge.
• Centrifuges are also used for separation of
macromolecules, separation of two immiscible liquids, to
concentrate small molecular species, and to
concentrate gases having different molecular weight.
• Centrifugation is widely employed when filtration
process is not applicable.
4. 4
APPLICATION
1. Centrifugation is widely used in bulk drug
industry to separate crystalline material from
suspension.
2. Centrifugation is used for isolation of bacterial
cells, and spores from liquid growth
3. Centrifugation is used for separation of blood
cells from blood
4. Ultracentrifuge is used for separation of virus
particle which are utilize in industrial applications
5. 5
APPLICATION
5. Centrifugation is used for separating drug present
in blood, urine and tissue fluid. This is essential for
evaluation of pharmacokinetic parameters and
biocquivalence studie
6. Centrifugation is used to obtain pure form of
insulin by selective precipitating other fraction of
protein and separating them by ultracentrifugation
7. Ultracentrifugation is used to determine molecular
weight of polymers, scrum albumin, insulin and
methyl cellulose
6. 6
FACTOR AFFECTING CENTRFUGATION
• Liquid viscosity
• Liquid density
• Solids concentration
• Particle size distribution
• Surface charge of particles
• Type and shape of particles
7. 7
FACTOR AFFECTING CENTRFUGATION
• Feed rate
• Agitation speed
• Size of centrifuge
• Height of cake
• Mode of operation
• Time at full speed
