4. ⦁ Proteome defines the complete set of
proteins expressed during a cell’s entire
lifetime.
⦁ Proteomics is the study of the proteome; it
uses technologies ranging from genetic
analysis to mass spectrometry.
⦁ Proteomics assesses activities, modifications,
localization, and interactions of proteins in
complexes.
6. ⦁ Proteome indicates the total proteins expressed by a
genome in a cell or tissue.
⦁ Biomarkers detection might allow identification of
patients who would benefit from further evaluation.
⦁ With the development of proteomic techniques,
proteome analysis provides a fast, non-invasive
diagnostic tool for patients with various diseases.
⦁ The advent of highly sensitive proteomic technologies
can identify proteins associated with development of
diseases well before any clinically identifiable alteration.
⦁ MS has a high resolving power and identifies proteins
with more accuracy.
7. 1. Structural Proteomics:- The ultimate aim of this
proteomics is to build a body of structural information
that will help predict the probable structure and potential
function for almost any protein from knowledge of its
coding sequence.
2. Functional proteomics:- It refers to the use of
proteomics techniques to analyze the characteristics of
molecular protein-networks involved in a living cell.
3. Expression proteomics:- It refers to the quantitative
study of protein expression between sample differing by
some variable.
8. ⚫Separation of proteins
One dimentional electrophoressis
2 D electrophossis(modern)
Multi-dimensional HPLC (modern)
⚫Analysis of proteins
Mass Spectrometry (modern)
⚫Database utilization
9. 1. Sample collection, handling and storage.
2. Separation of individual proteins by 2-D
electrophoresis.
3. Protein characterization.
4. Identification by mass spectrometry or other
methods.
5. Storage, manipulation, and comparison of the
data using bioinformatics.
10. ⦁ The large-scale analysis of protein
⦁ Protein structural comparisons can help to
identify the function of newly discovered
genes
⦁ X-ray crystallography, NMR spectroscopy.
11. ⦁ 1 one – Dimensional SDS-PAGE
⦁ 2 Two- Dimensional SDS-PAGE
⦁ What is SDS-PAGE? – SDS-PAGE a type of gel
electrophoresis.
⦁ What is the purpose of doing gel electrophoresis? –
It has been seen that by running a gel we are able to
identify more proteins from the sample.
⦁ An electric current is applied across the gel, causing
proteins will differentially migrate based on their
molecular mass.
12.
13. ⦁ 2-D gel electrophoresis a method for the separation and
identification of proteins in a sample by displacement in 2
dimensions.
⦁ First step is to separate based on charge or isoelectric
point, called isoelectric focusing.
⦁ Then separate based on size (SDS-PAGE).
14.
15. Application of Proteomics
⦁ Protein sample identification/ confirmation.
⦁ Protein sample purity determination.
⦁ Detection of post-translational modifications
⦁ Detection of amino acids substitution.
⦁ Mass fingerprint identification of proteins.
⦁ Nutrition Research
⦁ To identify unknown protein of intrest.
⦁ Quantify protein and peptide.
⦁ Protein Biomarker.
