This slide summarises the recent advancement in the field of protein Array. It includes a cell free expression system, Nucleic acid programmable protein array, nano-well array and other techniques.

1. NEXT-GENERATION HIGH-DENSITY
SELF-ASSEMBLING
FUNCTIONAL PROTEIN ARRAYS
By
Arindam Sain

2.  Serum screening to identify antibody immune responses for
cancer
 Detect post-translational modifications such as
phosphorylation
 Identify protein-protein or protein-DNA interactions
 High throughput quantification of protein binding kinetics
by coupling Protein array with surface plasmon resonance
(SPRi)
APPLICATIONS

3. CONVENTIONAL PROTEIN MICROARRAY

4. NEXT GENERATION CELL FREE PROTEIN
MICROARRAY
 Replaces printing proteins with the more reliable and less expensive
process of printing DNA
 Avoids the need to express, purify and store individual proteins
 Displays 95% of sequence-verified full-length genes
 Protein display levels are consistent from protein to protein(e.g.
differential MW)
 Assures protein integrity by using both mammalian expression
machinery and chaperone proteins to synthesize and fold proteins
 Easy to create custom arrays

5. MAMMALIAN CELL FREE EXPRESSION

6. NUCLEIC ACID PROGRAMMABLE PROTEIN
ARRAY-NAPPA
 Plasmid encoding target proteins fused with an affinity tag (e.g. GST)
are affixed to surface
 Array is activated by the addition of a cell free expression system
(IVTT mixture)
 Target proteins are expressed and immobilized in situ, and detected
using anti-tag antibody

7. WORK FLOW OF NAPPA

8. LaBaer et al

9. In 2016, cell-free protein microarrays made the jump
from bench to bedside with the launch of Provista’s
Videssa® test
The test, the first blood-based proteomics biomarker
assay for early breast cancer detection, includes 28
autoantibody biomarkers that were originally discovered
with cell-free protein arrays
Videssa® test can significantly reduce the number of
unnecessary breast biopsies
RECENT ADVANCEMENTS
Anderson et al.

10. POTENTIAL WORKFLOW

11. DRUG SCREENING
Rauf et al., 2018

13. THANK YOU