Proteins perform many functions in living organisms and differ based on their amino acid sequence. Affinity chromatography uses the interaction between proteins and ligands to separate proteins, and magnetic nanoparticles can be used to make this process faster and more efficient. In this experiment, magnetic nanoparticles were prepared and bound to tissue plasminogen activator to isolate it using affinity chromatography, washing and eluting steps. Absorbance readings would then indicate if proteins were successfully isolated.