Untargeted metabolomics, namely discovery metabolomics, involves the comparison of the metabolome between the control and test groups, to identify differences between their metabolite profiles which may be relevant to specific biological conditions.
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Plant metabolomics
1. plant metabolomics
• Untargeted metabolomics, namely discovery metabolomics, involves
the comparison of the metabolome between the control and test
groups, to identify differences between their metabolite profiles
which may be relevant to specific biological conditions.
2.
3. • There are usually three steps in the experimental workflow:
• Profiling in order to seek the metabolites with statistically significant variations in abundance within a set of
experimental and control samples;
• Determination of metabolite ID, including the chemical structure;
• Comprehensions as the last step which makes connections between the identified metabolites and the biological
processes.
• In the workflow of discovery metabolomics, analytical reproducibility is critical for expression profiling work;
annotation is a tentative identification based on an accurate mass match to a database or a spectral match to a
library; the collected data can be interpreted for biomarker discovery, biological signature/fingerprint selection and
pathway mapping. Above are the most important parts in untargeted metabolomic research. The tech panel in
Creative Proteomics is experienced in sample preparation, data interpretations. It also can provide you reliable
metabolite identification.