Analytical Profile of Coleus Forskohlii | Forskolin .pdf
APPLICATION OF METABOLOMICS.pdf
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INDEX
PAGE 1- COVER PAGE
PAGE 2- CONTENT SLIDE
PAGE 3- DEFINITION
PAGE 4- APPLICATION OF METABOLOMICS
PAGE 5- MORE ABOUT METABOLOMICS
PAGE 6- METABOLOMICS IN BIOINFORMATICS
PAGE 7- METABOLOMICS IN TARGET
IDENTIFICATION
PAGE 8- STEPS INVOLVED IN METABOLOMICS IN
TARGET IDENTIFICATION
PAGE 9- BIBLIOGRAPHY
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What is Metabolomics?
Metabolomics is the state-of-the-art qualitative or quantitative analysis of metabolites
present in biological samples.
As majority of diseases are associated with metabolic alterations – either as a cause
or result of the disease process – metabolomics is increasingly being used in drug
and biomarker discovery.
What is Target Identification?
Target identification is a crucial step in drug discovery and development, as it allows researchers to
understand the underlying mechanisms of a disease and to identify potential drug targets.
Metabolomics can be used in target identification by profiling the metabolites of cells or tissues that
have been treated with a drug or that are affected by a disease. This information can be used to
identify the pathways and proteins that are involved in the disease or drug response.
Metabolomics is a powerful technique for the comprehensive analysis of small molecule
metabolites in biological systems.
It has many applications in various fields:
Drug
discovery
Personalized
medicine
Environmental
studies
TARGET
IDENTIFICATION
Nutritional
Research
Microbial
metabolism
Biomarker
discovery
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It is a rapidly growing field of study that focuses on the identification and quantification of
small molecules in biological systems.
Here are some of the applications of metabolomics:
1. Biomarker discovery: Metabolomics can be used to identify novel biomarkers for the
early detection, diagnosis, and monitoring of diseases. By comparing the metabolite
profiles of healthy and diseased individuals, researchers can identify specific
metabolites that are associated with certain diseases.
2. Drug development: Metabolomics can be used in drug discovery to identify new drug
targets and to evaluate the efficacy and safety of drug candidates. Metabolite profiling
can provide information on the metabolic pathways that are affected by drugs, as well
as potential side effects.
3. Nutritional research: Metabolomics can be used to study the effects of diet and
nutrition on human health. By analyzing the metabolite profiles of individuals on
different diets, researchers can gain insights into the metabolic processes that are
affected by diet and identify potential biomarkers for dietary intake.
4. Environmental toxicology: Metabolomics can be used to study the effects of
environmental toxins on human health. By analyzing the metabolite profiles of
individuals exposed to environmental toxins, researchers can identify specific
metabolites that are associated with exposure and potential health effects.
5. Microbial metabolism: Metabolomics can be used to study the metabolism of
microorganisms, including bacteria, fungi, and viruses. By analyzing the metabolite
profiles of these organisms, researchers can gain insights into their metabolic
processes, identify potential drug targets, and develop new antimicrobial therapies.
6. Agricultural research: Metabolomics can be used to study plant metabolism and to
identify biomarkers for plant quality, yield, and disease resistance. By analyzing the
metabolite profiles of crops, researchers can identify specific metabolites that are
associated with these traits and develop strategies to improve crop production.
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One of the most important applications of metabolomics is target identification, which
involves the identification of the molecular targets that are affected by a particular drug or
disease.
Metabolomics can also be used to identify potential biomarkers for disease diagnosis and
monitoring. By analyzing the metabolites in biological samples such as blood or urine,
researchers can identify changes in metabolite levels that are associated with a particular
disease. These biomarkers can be used to develop diagnostic tests and to monitor the
progression of the disease or the effectiveness of a particular treatment.
In addition to drug discovery and biomarker identification, metabolomics has applications in
environmental studies. It can be used to study the metabolic response of organisms to
environmental stressors such as pollution or climate change. This information can be used to
develop strategies for environmental monitoring and remediation.
Most diseases result in metabolic changes. In many cases, these changes play a causative role
in disease progression. By identifying pathological metabolic changes, metabolomics can
point to potential new sites for therapeutic intervention. Particularly promising enzymatic
targets are those that carry increased flux in the disease state. Definitive assessment of flux
requires the use of isotope tracers. Here we present techniques for finding new drug targets
using metabolomics and isotope tracers. The utility of these methods is exemplified in the
study of three different viral pathogens. For influenza A and herpes simplex virus,
metabolomic analysis of infected versus mock-infected cells revealed dramatic concentration
changes around the current antiviral target enzymes. Similar analysis of human-
cytomegalovirus-infected cells, however, found the greatest changes in a region of
metabolism unrelated to the current antiviral target. Instead, it pointed to the tricarboxylic
acid (TCA) cycle and its efflux to feed fatty acid biosynthesis as a potential preferred target.
Isotope tracer studies revealed that cytomegalovirus greatly increases flux through the key
fatty acid metabolic enzyme acetyl-coenzyme A carboxylase. Inhibition of this enzyme
blocks human cytomegalovirus replication. Examples where metabolomics has contributed to
identification of anticancer drug targets are also discussed. Eventual proof of the value of
metabolomics as a drug target discovery strategy will be successful clinical development of
therapeutics hitting these new targets
Metabolites are essential components of every living organism, providing cellular energy as
well as structural and signalling functions. For example, metabolites encompass biochemical
classes such as nucleotides, amino acids, carbohydrates and lipids, which are the building
blocks of DNA/RNA, proteins, glycogen and cellular membranes, respectively. Metabolites
are both end products of metabolism (eg, enzymatic activities) and derive from the
environment (eg, gut microbiota, diet, medications). Changes in the levels or composition of
metabolites determine the phenotype of a biological system, which is associated with a
specific physiological and pathological state.
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METABOLOMICS IN BIOINFORMATICS-
Metabolomics has become an important tool in bioinformatics due to its ability to generate
large amounts of complex data that can be analyzed using computational methods. The
integration of metabolomics with bioinformatics can provide a comprehensive understanding
of metabolic pathways, regulatory networks, and the interactions between metabolites, genes,
and proteins.
Here are some of the uses of metabolomics in bioinformatics:
1. Data processing and analysis: Metabolomics generates large amounts of data that
require sophisticated computational tools to process and analyze. Bioinformatics tools
such as statistical analysis, machine learning algorithms, and data visualization
software can be used to analyze and interpret metabolomics data.
2. Metabolic pathway analysis: Metabolomics can be used to identify metabolic
pathways and to study the interactions between metabolites and enzymes.
Bioinformatics tools such as pathway analysis software can be used to integrate
metabolomics data with genomic and proteomic data to provide a comprehensive
understanding of metabolic pathways and networks.
3. Biomarker identification: Metabolomics can be used to identify biomarkers for
disease diagnosis and monitoring. Bioinformatics tools such as data mining
algorithms and network analysis can be used to identify metabolites that are
associated with a particular disease or condition.
4. Systems biology: Metabolomics can be integrated with other omics data such as
genomics, transcriptomics, and proteomics to provide a systems-level understanding
of biological processes. Bioinformatics tools such as network analysis and pathway
mapping can be used to integrate and analyze multiple omics data sets.
Overall, the integration of metabolomics with bioinformatics can provide a powerful
approach for understanding complex biological systems and for developing new insights into
the underlying mechanisms of diseases and other biological processes.
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METABOLOMICS IN TARGET IDENTIFICATION-
Target identification is the process of identifying the molecular targets of a drug or disease. It
is an important step in drug discovery and development, as it allows researchers to
understand the underlying mechanisms of a disease and to identify potential drug targets.
Bioinformatics plays a critical role in target identification by integrating data from multiple
sources and using computational tools to analyze and interpret the data.
Here are some of the ways bioinformatics can be used in target identification:
1. Genomics: Bioinformatics tools can be used to analyze genomic data to identify
potential drug targets. This can involve identifying genes that are overexpressed in
disease states or that are involved in specific metabolic pathways.
2. Proteomics: Proteomics can be used to identify proteins that are involved in disease
pathways or that interact with potential drug molecules. Bioinformatics tools such as
protein-protein interaction databases and network analysis software can be used to
identify potential drug targets.
3. Metabolomics: Metabolomics can be used to identify changes in metabolite levels that
are associated with a particular disease or drug treatment. Bioinformatics tools such as
pathway analysis software can be used to identify the metabolic pathways that are
affected by a drug or disease, and to identify potential drug targets within those
pathways.
4. Data integration: Bioinformatics tools can be used to integrate data from multiple
sources, including genomics, proteomics, and metabolomics, to provide a
comprehensive understanding of disease pathways and potential drug targets. This can
involve the use of network analysis, pathway mapping, and other computational tools
to identify key nodes within complex biological networks.
Overall, bioinformatics plays a critical role in target identification by integrating data from
multiple sources and using computational tools to analyze and interpret the data. This
approach can lead to the identification of new drug targets and the development of more
effective therapies for a wide range of diseases.
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Steps involved in using metabolomics in target identification:
1. Sample collection and preparation: Metabolomics analysis typically involves the
collection and preparation of biological samples such as blood, urine, or tissues. The
samples are typically processed using techniques such as liquid chromatography and
mass spectrometry to detect and quantify metabolites.
2. Metabolite identification: The metabolites detected in the samples are typically
identified using bioinformatics tools such as mass spectrometry databases and
metabolic pathway databases. This can involve comparing the mass spectrometry data
to reference metabolite databases or using computational tools to identify the
metabolic pathways that are affected by a particular disease or drug treatment.
3. Metabolic pathway analysis: Once the metabolites have been identified, bioinformatics
tools can be used to analyze the metabolic pathways that are affected by a particular
disease or drug treatment. This can involve using pathway analysis software to identify
key nodes within the metabolic network and to predict the effects of perturbations to
the network.
4. Target identification: The metabolic pathways that are affected by a particular disease
or drug treatment can provide clues to potential drug targets. For example, if a
particular enzyme is identified as a key node within a metabolic pathway, it may be a
potential drug target. Bioinformatics tools can be used to identify potential drug targets
within the metabolic network and to predict the effects of targeting those targets.
Metabolomics can be used in target identification by identifying metabolites that are
associated with a particular disease state or drug treatment.
Overall, metabolomics can be a powerful tool in target identification by providing a
comprehensive understanding of the metabolic pathways that are affected by a particular
disease or drug treatment. This can lead to the identification of new drug targets and the
development of more effective therapies for a wide range of diseases.
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BIOBLIOGRAPHY
1. Wishart, D. S. (2008). Metabolomics: applications to food science and nutrition
research. Trends in food science & technology, 19(9), 482-493.
2. Johnson, C. H., Ivanisevic, J., & Siuzdak, G. (2016). Metabolomics: beyond
biomarkers and towards mechanisms. Nature reviews Molecular cell biology, 17(7),
451-459.
3. Wang, Y., Ji, P., Liu, J., & Sun, W. (2017). Metabolomics in the identification of
metabolic pathways and targets in cancer. Frontiers in oncology, 7, 149.
4. Xiao, J. F., Zhou, B., Ressom, H. W. (2012). Metabolite identification and
quantitation in LC-MS/MS-based metabolomics. TrAC Trends in Analytical
Chemistry, 32, 1-14.
5. Yang, K., & Han, X. (2016). Lipidomics: techniques, applications, and outcomes
related to biomedical sciences. Trends in biochemical sciences, 41(11), 954-969.
6. Zhang, X., Zhao, Y., & Xia, J. (2016). Metabolomics in the frontier of personalized
medicine. Biomedical reports, 4(1), 5-7.
7. Yin, P., & Xu, G. (2014). Current state-of-the-art of nontargeted metabolomics based
on liquid chromatography-mass spectrometry with special emphasis in clinical
applications. Journal of chromatography A, 1374, 1-13.
8. Sreekumar, A., Poisson, L. M., Rajendiran, T. M., Khan, A. P., Cao, Q., Yu, J., ... &
Chinnaiyan, A. M. (2009). Metabolomic profiles delineate potential role for sarcosine
in prostate cancer progression. Nature, 457(7231), 910-914.
9. Li, S., Park, Y., Duraisingham, S., Strobel, F. H., Khan, N., Soltow, Q. A., ... & Jones,
D. P. (2013). Predicting network activity from high throughput metabolomics. PLoS
computational biology, 9(7), e1003123.
10. Kind, T., & Fiehn, O. (2010). Advances in structure elucidation of small molecules
using mass spectrometry. Bioanalytical reviews, 2(1-4), 23-60.