3. Metabolites are the intermediates
and products of metabolism.
The measurement and
interpretation of the endogenous
metabolite profile from a
biological sample (typically urine,
serum, or biological tissue extract)
have provided many opportunities
to investigate drug and metabolite
that are present in body.
4. DMPK studies is most
important part during
process of drug
discovery,
in-vitro ADMET
screens, thereby
accelerating the drug
discovery process.
DRUG DISCOVERY
13. LC-MS/MS
Mass spectrometry plays a key
role in drug metabolite
identification,
It has improved accuracy and
stability, along with new data
processing techniques, has
improved the quality and
productivity of metabolite
identification processes.
LC-MS/MS
14.
15. •Incubation of test drug sample with hepatocytes/HLM will lead to
formation of Phase 1 (Oxidation / Reduction / hydrolysis) and
Phase 2 conjugated metabolites.
•Which will be identified by validated LC-MS/MS method.
•Metabolism (percentage of test compound metabolized after a
certain time) and qualitative determination of metabolite pattern
(minor, intermediate, and major abundance of each metabolite)
EXPECTED OUTCOMES