ZnO NPs synthesized by chemical wet method and green method

1. Role of synthesized
Zinc Oxide nanoparticles on the germination
and growth characteristics of
Mungbean seeds (Vigna radiata)
Presented by: Camellia Roy
3rd year B.Sc Biotechnology (H)
Gurunanak Institute of Pharmaceutical
Science & Technology

2. Overview
 Nanotechnology
 Synthesis of Zinc oxide : i) Chemical wet method
ii) Green synthesis method
The beneficial effect of Zinc oxide nanoparticles
prepared for the treatment of mungbean (Vigna
radiata) seeds.
 Results and Discussions
Conclusion
References

3. One nanometer is a billionth of a meter, or 10-9
of a
meter.
Examples:
Figure 1: Illustrative examples of nanotechnology
Meet Nano!

4. Physicist Richard Feynman, the father of
nanotechnology “There’s Plenty of Room at the
Bottom ”
Professor Norio Taniguchi coined the term
nanotechnology.

5. Synthesis of Zinc oxide nanoparticles
 Chemical wet method
ZnCl2 + 2NaOH Zn (OH)2 + 2NaCl
Zn (OH)2 ZnO + H2O
Figure 2: Nano Zinc oxide particles synthesized by
chemical wet method

6. Continue.
 Green synthesis method
BA
C
D
Figure 3: Synthesis of ZnO Nanoparticles using leaf
extract of Camellia sinensis (green tea)

7. The beneficial effect of Zinc oxide
nanoparticles prepared for the treatment of
mungbean (Vigna radiata) seeds
BA C
Figure 4: A) Mungbean seeds (B) Seedling exposure
(C) Mungbean sprouts

8. Results and discussions
Figure 5: A) Different concentrations of ZnO NPs
on Mungbean seed germination (B) Effect of ZnO
NPs on seed germination
A B

9. Biomass
weight
(gm)
Shoot
Length
(cm)
Root
Length
(cm)
Shoot
Weight
(gm)
Root
Weight
(gm)
Control 0.425 20.4 7.4 0.291 0.020
50 mg 0.536 22.2 8.9 0.322 0.022.8
100 mg 0.532 23.5 9 0.332 0.023
150 mg 0.526 20.4 9.4 0.318 0.020.2
Continue..
Figure 6: Mungbean seed germination and
measurement of growth parameters within 20 days

10. Conclusion
Environment eco-friendly synthesized zinc oxide nanoparticles effect
on the mung bean seed germination.
It penetrates easily inside the plant cell wall and causes effects on
biomass and growth parameters of mung bean plant.
Deposition of small size nanoparticles inside the cell wall and vacuoles
causes disturbance in metabolic activity of plant.
Mungbean (Vigna radiata) are economically important crop plant,
nanoparticles can easily find their way in human body through food
chain.
More investigations are needed to determine the positive and
negative impact of nanoparticles on crop plant and its consequences in
other living organisms.

11. References
A Khorsand Zak, R Razali, WH Abd Majid, Majid Darroudi, (2011).
Synthesis and Characterization of a Narrow size Distribution of Zinc
Oxide Nanoparticles, International Journal of Nano medicine, Vol, 6, pp.
1399–1403.
F.Hong, L. Wang, X. Meng, Z. wei, G. (2005) Zhao, Effect of Nano-
TiO2 on photochemical reaction of chloroplasts of spinch, Biological
Trace Elements Resaerch, vol.8
Gianquinto G, Azmi AR, Tola LD, (2000). Interaction effects of
phosphorus and zinc on photosynthesis, growth and yield of dwarf bean
grown in two environments. Plant Soil, vol.28.
K. Suresh babu and V. Narayanan, (2013). Hydrothermal Synthesis of
Hydrated Zinc Oxide Nanoparticles and its Characterization Chem Sci
Trans., Vol, 1 pp. S33-S36

12. .
Navarro, E., Baun, A., Behra, R., Hartmma N.B., Filser,
(2008) Environmental behavior and eco-toxicity of
engineered nanoparticles to algae, plants and fungi.
Ecotoxicology, vol. 17.
Liu XM, Zhang FD, Zhang SQ, (2010). Effects of nano-
ferric oxide on the growth and nutrients absorption of
peanut. Plant Nutr Fert Sci, vol. 11.
S.R. Senthilkumar, T. Sivakumar, (2014).Green tea(Camellia
sinensis) mediated synthesis of Zinc oxide (ZnO)
nanoparticles and studies on their antimicrobial activities, Vol
6,Issue 6,ISSN-0975-1419
 K. Prasad1, Anal K. Jha.(2009), ZnO
Nanoparticles,Synthesis and Adsorption Study. Natural
Science,Vol, 1,pp. 129-135

13. THANK YOU