Semi polar solvents & their properties used in thin layer chromatography(chloroform)

1. Pharmacognosy & Phytochemistry- II(Practical)
Submitted by –
Mr. Suyash Jain
Department Of Pharmaceutical Sciences
Dr. Harisingh Gour Vishwavidyalaya, Sagar (M.P.)
Chloroform
( A Central University )
Semi-Polar solvents & their properties used in Thin layer Chromatography

2. Various Semi Polar Solvents used in Thin Layer
Chromatogrephy
1.Acetone
2.Ethyl Acetate
3.Dichloromethane
4.Diethyl Ether
5.Chloroform
6.T-Butyl Ether

3. 1. IUPAC NAME:- Trichloromethane
2. Molecular Weight:- 119.37
3. Boiling point:- 61.2 °C
4. Melting point:- 63.5 °C
5. Density:- 1.49 g/cm³
6. Appearance:- Colorless liquid
7. Odor:-
8. Molecular shape:- Tetrahedral
9. Dipole moment:- 1.15 D
10. Solubility:- Insoluble in water
11. Polarity Index:- 4.1
Misleadingly-pleasant
ethereal odor, leading
to olfactory fatigue

4. Prepare TLC Solvent Mixture with
Chloroform
65:25:4 (v/v/v)
chloroform : methanol: water
65:25:4 (v/v/v)
chloroform : methanol: ammonium
hydroxide