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DEFINITION
•Puncture of the subarachnoid space in the
lumbar region of the spinal cord to withdraw
cerebrospinal fluid or inject drugs.
PURPOSES
• collect a sample of cerebrospinal fluid
• measure the pressure of fluid in the spinal canal
• inject drugs
• To detect
• bacterial, fungal and viral infections, including meningitis,
encephalitis and syphilis
• bleeding around the brain (subarachnoid hemorrhage)
• cancers involving the brain and spinal cord
• inflammatory conditions of the nervous system, including
Guillain-Barre syndrome and multiple sclerosis.
INDICATIONS
• Diagnosis of diseases with altered CSF composition
• Intrathecal administration of pharmaceuticals
• CSF pressure measurement
NEEDLE TYPES
NEEDLE FOR LP
GAUGE (mm) COLOR CODE
16G (1.6 mm) White
18G (1.2 mm) Pink
19G (1.1 mm) Cream
20G (0.9 mm) Yellow
21G (0.8mm) Deep green
22G (0.7 mm) Black
24G (0.6 mm) Deep blue
25G (0.5 mm) Orange
26G (0.45mm) Brown
27G (0.4mm)
SPINAL NEEDLE SIZES
Parameter Normal value
Total volume 150 ml
CSF pressure 6- 14 mmof HG
Specific gravity 1.006 – 1.008
Glucose 40 – 70 mg/dl
Proteins 20 – 40 mg/dl
Lactate 1 – 2mmol/L
Red blood cells None
White blood cells 0 – 5 cells/ microliter
ARTICLES
• STERILE DRESSING
• STERILE GLOVES
• STERILE DRAPE
• ANTISEPTIC SOLUTION WITH SKIN
SWABS
• LIDOCAINE 1% WITHOUT
EPINEPHRINE
• SYRINGE, 3 ML
• NEEDLES, 20 AND 25 GAUGE
• SPINAL NEEDLES, 20 AND 22 GAUGE
• THREE-WAY STOPCOCK
• MANOMETER
• FOUR PLASTIC TEST TUBES,
COMPLICATIONS
• Post–spinal puncture headache
Headache is the most common
complication of lumbar puncture,
observed in 20-70% of patients. It
usually begins 24-48 hours after the
procedure and is more common in
young adults.
2) BLOODY TAP
3) DRY TAP
4)INFECTION
•Cellulitis, skin abscesses, epidural
abscesses, spinal abscesses, and discitis
can result from a contaminated spinal
needle
5)HEMORRHAGE
DYSESTHESIA
IRRITATION OF NERVES OR NERVE
ROOTS BY THE SPINAL NEEDLE CAN
CAUSE DIFFERENT LOWER-EXTREMITY
DYSESTHESIAS.
6) POST–DURAL PUNCTURE CEREBRAL
HERNIATION
• withdrawal of fluid when initial pressures are abnormal
could result in spinal cord compression or cerebral
herniation
Lumbar puncture

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Lumbar puncture

  • 1.
  • 2. DEFINITION •Puncture of the subarachnoid space in the lumbar region of the spinal cord to withdraw cerebrospinal fluid or inject drugs.
  • 3.
  • 4. PURPOSES • collect a sample of cerebrospinal fluid • measure the pressure of fluid in the spinal canal • inject drugs • To detect • bacterial, fungal and viral infections, including meningitis, encephalitis and syphilis • bleeding around the brain (subarachnoid hemorrhage) • cancers involving the brain and spinal cord • inflammatory conditions of the nervous system, including Guillain-Barre syndrome and multiple sclerosis.
  • 5.
  • 6.
  • 7.
  • 8. INDICATIONS • Diagnosis of diseases with altered CSF composition • Intrathecal administration of pharmaceuticals • CSF pressure measurement
  • 11.
  • 12. GAUGE (mm) COLOR CODE 16G (1.6 mm) White 18G (1.2 mm) Pink 19G (1.1 mm) Cream 20G (0.9 mm) Yellow 21G (0.8mm) Deep green 22G (0.7 mm) Black 24G (0.6 mm) Deep blue 25G (0.5 mm) Orange 26G (0.45mm) Brown 27G (0.4mm) SPINAL NEEDLE SIZES
  • 13. Parameter Normal value Total volume 150 ml CSF pressure 6- 14 mmof HG Specific gravity 1.006 – 1.008 Glucose 40 – 70 mg/dl Proteins 20 – 40 mg/dl Lactate 1 – 2mmol/L Red blood cells None White blood cells 0 – 5 cells/ microliter
  • 14. ARTICLES • STERILE DRESSING • STERILE GLOVES • STERILE DRAPE • ANTISEPTIC SOLUTION WITH SKIN SWABS • LIDOCAINE 1% WITHOUT EPINEPHRINE • SYRINGE, 3 ML • NEEDLES, 20 AND 25 GAUGE • SPINAL NEEDLES, 20 AND 22 GAUGE • THREE-WAY STOPCOCK • MANOMETER • FOUR PLASTIC TEST TUBES,
  • 15. COMPLICATIONS • Post–spinal puncture headache Headache is the most common complication of lumbar puncture, observed in 20-70% of patients. It usually begins 24-48 hours after the procedure and is more common in young adults.
  • 18. 4)INFECTION •Cellulitis, skin abscesses, epidural abscesses, spinal abscesses, and discitis can result from a contaminated spinal needle
  • 20. DYSESTHESIA IRRITATION OF NERVES OR NERVE ROOTS BY THE SPINAL NEEDLE CAN CAUSE DIFFERENT LOWER-EXTREMITY DYSESTHESIAS.
  • 21. 6) POST–DURAL PUNCTURE CEREBRAL HERNIATION • withdrawal of fluid when initial pressures are abnormal could result in spinal cord compression or cerebral herniation