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-BY KHAN RAHEELA
MSC – 1
ANALYTICAL
CHEMISTRY
CONTENT :
PRINCIPLE OF AAS .
HOW IT WORKS .
INSTRUMENTATION .
APPLICATIONS .
INTRODUCTION :
Atomic absorption spectroscopy is the most common
technique used for detecting metal & metalloid in
samples .
It is very reliable & simple to use.
 It can analyze over 62 elements .
It is also helpful to find concentrations of metal in
sample .
PRINCIPLE :
Atomic absorption spectrometry (AAS) is a technique
in which free gaseous atoms absorb electromagnetic
radiation at a specific wavelength to produce a
measurable signal.
The absorption signal is proportional to the
concentration of those free absorbing atoms in the
optical path. Therefore, for AAS measurements the
analyte must be first converted into gaseous atoms,
usually by application of heat to a cell called atomizer.
The type of atomizer defines the two main AAS-based
analytical techniques: flame AAS (FAAS) that
provides analytical signals in a continuous fashion
and electrothermal AAS (ETAAS) delivering analytical
signals in a discontinuous mode.
In both cases, liquid (or dissolved) samples
are easily introduced into the analyzer, as
an aerosol in the case of FAAS or as well-
defined low microliter volumes in ETAAS.
Furthermore, the coupling of hydride
generation and cold vapour methods allow
the introduction of analytes in the
atomizer as a gas phase.
INSTRUMENTATION :
HOLLOW CATHODE LAMP .
NEBULIZER .
ATOMIZER .
MONOCHROMATOR .
DETECTOR .
REFERENCES :
 https://www.slideshare.net/sharmasuriti/atomic-
absorption-spectroscopy-15185397?from_m_app=android
 https://www.google.com/search?q=photomultiplier+tub
e&rlz=1C1CHBD_enIN739IN751&source
 https://www.sciencedirect.com/topics/materials-
science/atomic-absorption-spectrometry
THANK YOU

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Atomic absorption spectroscopy

  • 1. -BY KHAN RAHEELA MSC – 1 ANALYTICAL CHEMISTRY
  • 2. CONTENT : PRINCIPLE OF AAS . HOW IT WORKS . INSTRUMENTATION . APPLICATIONS .
  • 3.
  • 4. INTRODUCTION : Atomic absorption spectroscopy is the most common technique used for detecting metal & metalloid in samples . It is very reliable & simple to use.  It can analyze over 62 elements . It is also helpful to find concentrations of metal in sample .
  • 5.
  • 6. PRINCIPLE : Atomic absorption spectrometry (AAS) is a technique in which free gaseous atoms absorb electromagnetic radiation at a specific wavelength to produce a measurable signal. The absorption signal is proportional to the concentration of those free absorbing atoms in the optical path. Therefore, for AAS measurements the analyte must be first converted into gaseous atoms, usually by application of heat to a cell called atomizer. The type of atomizer defines the two main AAS-based analytical techniques: flame AAS (FAAS) that provides analytical signals in a continuous fashion and electrothermal AAS (ETAAS) delivering analytical signals in a discontinuous mode.
  • 7. In both cases, liquid (or dissolved) samples are easily introduced into the analyzer, as an aerosol in the case of FAAS or as well- defined low microliter volumes in ETAAS. Furthermore, the coupling of hydride generation and cold vapour methods allow the introduction of analytes in the atomizer as a gas phase.
  • 8. INSTRUMENTATION : HOLLOW CATHODE LAMP . NEBULIZER . ATOMIZER . MONOCHROMATOR . DETECTOR .
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18.
  • 19.
  • 20.
  • 21.
  • 22.
  • 23.
  • 24. REFERENCES :  https://www.slideshare.net/sharmasuriti/atomic- absorption-spectroscopy-15185397?from_m_app=android  https://www.google.com/search?q=photomultiplier+tub e&rlz=1C1CHBD_enIN739IN751&source  https://www.sciencedirect.com/topics/materials- science/atomic-absorption-spectrometry