A simple total ash determination provides very little information about the exact mineral profile of the feed/food. Analytical techniques involving spectroscopy are generally used to obtain the macro and micro mineral contents except the phosphorus. Atomic absorption spectroscopy is a very common technique for detecting metals and metalloids. It is very reliable and simple to use. It can analyze over 62 elements. It also measures the concentration of metals in the sample. First AAS was built by CSIRO scientist Alan Walsh in 1954 The technique makes use of the atomic absorption spectrum of a sample in order to assess the concentration of specific analyte within it. It requires standards with known analyte content to establish the relation between the measured absorbance and the analyte concentration and relies therefore on the Beer-Lambert law. For instrumentation, flame, non-flame, and graphite furnace are available in atomic absorption instruments. Any AAS instrumentation has the following types of components:- Atomization Hollow cathode lamp Monochromator Detector Recorder For instrumentation, flame, non-flame, and graphite furnace are available in atomic absorption instruments. Any AAS instrumentation has the following types of components:- Atomization Hollow cathode lamp Monochromator Detector Recorder For instrumentation, flame, non-flame, and graphite furnace are available in atomic absorption instruments. Any AAS instrumentation has the following types of components:- Atomization Hollow cathode lamp Monochromator Detector Recorder For instrumentation, flame, non-flame, and graphite furnace are available in atomic absorption instruments. Any AAS instrumentation has the following types of components:- Atomization Hollow cathode lamp Monochromator Detector Recorder For instrumentation, flame, non-flame, and graphite furnace are available in atomic absorption instruments. Any AAS instrumentation has the following types of components:- Atomization Hollow cathode lamp Monochromator Detector Recorder For instrumentation, flame, non-flame, and graphite furnace are available in atomic absorption instruments. Any AAS instrumentation has the following types of components:- Atomization Hollow cathode lamp Monochromator Detector Recorder For instrumentation, flame, non-flame, and graphite furnace are available in atomic absorption instruments. Any AAS instrumentation has the following types of components:- Atomization Hollow cathode lamp Monochromator Detector Recorder For instrumentation, flame, non-flame, and graphite furnace are available in atomic absorption instruments. Any AAS instrumentation has the following types of components:- Atomization Hollow cathode lamp Monochromator Detector Recorder VFor instrumentation, flame, non-flame, and graphite furnace are available in atomic absorption instruments. Any AAS instrumentation has the following types of components:- Atomization Hollow cathode lamp Monochromato

1. Atomic Absorption
Spectroscopy
ANN-608
Research Techniques in Animal Nutrition
Dr. Rahul dangi
RAHUL DANGI

2. Introduction
A simple total ash determination
provides very little information
about the exact mineral profile of
the feed/food. Analytical technique
involving spectroscopy are
generally used to obtain the macro
and micro mineral contents except
the phosphorus.
RAHUL DANGI

3. Atomic absorption spectroscopy is a
very common technique for
detecting metals and metalloids.
It is very reliable and simple to
use.
It can analyze over 62 elements.
It also measures the concentration
of metals in the sample.
First AAS was built by CSIRO
scientist Alan Walsh in 1954
RAHUL DANGI

4. Principle
The technique makes use of the
atomic absorption spectrum of a
sample in order to assess the
concentration of specific analyte
within it. It requires standards
with known analyte content to
establish the relation between the
measured absorbance and the
analyte concentration and relies
therefore on the Beer-Lambert law.
RAHUL DANGI

5. The techniques uses basically the
principle that free atoms(gas) generated
in an atomizer can absorb radiation at
specific frequency.
AAS quantifies the absorption of ground
state atoms in the gaseous state.
The atoms absorb UV or visible light
and make transitions to higher
electronic energy levels. The analyte
concentration is determined from the
amount of absorption.
RAHUL DANGI

6. Concentration measurement are
usually determined from a working
curve after calibrating the
instrument with standards of
known concentration.
Atomic absorption is a very
common technique for detecting
metals and metalloids in
environmental samples.
RAHUL DANGI

7. RAHUL DANGI

8. Instrumentation
For instrumentation, flame, non-
flame, and graphite furnace are
available in atomic absorption
instruments.
Any AAS instrumentation has the
following types of components:-
Atomization
Hollow cathode lamp
Monochromator
Detector
Recorder
RAHUL DANGI

9. Atomization
It can be carried out either by a
flame or furnace. Heat energy is
utilized in atomics absorption
spectroscopy to convert metallic
elements to atomic dissociated
vapour. The temperature should be
controlled very carefully for the
conversion of atomic vapour. At too
high temp. atoms can be ionized.
RAHUL DANGI

10. Hollowcathode
lamp
We need a continuous source of
radiation in the AAS instruments.
The extreme narrowness of the
absorption line in the sources
causes problems. We used a hollow
cathode glow discharge lamp to
give sharp emission lines for a
specific elements in AAS
instrumentation.
RAHUL DANGI

11. Monochromato
r
 It is an optical device that transmits a
narrow band of wavelengths of light
or other radiation from a wider range
of wavelengths. The atoms in the AAS
instrument accept the energy of
excitation and emit radiation. A
desired band of lines can be isolated
with a monochromator by passing a
narrow band. The spectra through a
monochromator can be shown by a
curve.
RAHUL DANGI

12. Detector
A detector can convert light coming
from a monochromator to a
simplified electrical signal.
Generally, we used a
photomultiplier tube as a detector
in the AAS instrument. A detector
can be tuned to respond by a
specific wavelength or frequency.
RAHUL DANGI

13. Recorder
The recorder can receive electrical
signals from the detector to convert
them into a readable response. In
atomic absorption spectroscopy
instrumentation, today we used a
computer system with suitable
software for recording signals
coming from the detector.
RAHUL DANGI

14. Applications
 Determination of even small amounts
of metals (lead, mercury, calcium,
magnesium etc.) as follows:-
 Environmental studies: drinking
water, ocean water, soil.
 Food industry.
 Pharmaceutical industry.
 Today, the AAS technique is the most
powerful tool in analytical chemistry,
forensic science.
RAHUL DANGI

15.  The most important advantage is the
speed of analysis. It can analyze various
samples within a day.
 Secondly, it is possible to determine all
elements at trace concentration.
 Thirdly, it is not always essential to
separate the element before analysis
because AAS can be used to determine
one element in presence of another.
 The AAS principle or instrumentation
can be used to analyze 67 metals and
several non metals such as phosphorus &
Boron.
RAHUL DANGI

16. Thank you
RAHUL DANGI