Goat anti Human IgM antibody recognizes the heavy chain of human IgM. This antibody has been cross absorbed against human IgA and IgG. Goat anti Human IgM antibody might cross react with IgM from other species.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. For general protocol recommendations, please visit the antibody section.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Secondary antibodies are commonly used to detect and visualize the presence of a primary antibody in applications like western blotting or immunofluorescent histology. In these applications the secondary antibody is labeled with a reporter molecule, which may be an enzyme like HRP or a fluorophore such as FITC. Multiple secondary antibodies can bind to a single primary antibody increasing the sensitivity and amplifying the signal.
Further signal amplification can be achieved by using an unlabeled secondary and a labeled tertiary antibody if required.
Read our secondary antibodies optimization guide for essential information on experimental design.
2. Antibody capture:
Antibody capture:Unlabeled secondary antibodies can be used to capture antibodies of interest from biological solutions by quantitative ELISA. For example, an unlabeled secondary mouse anti-human IgG may be used as the capture antibody to bind human IgG from a patient serum sample. This is then detected with a labeled secondary mouse anti-human IgG which binds to the captured IgG.
The use of a calibration curve of known standards allows this signal to be quantified.
3. Detection and quantification of recombinant proteins:
Secondary antibodies can be used to detect and quantify recombinant proteins that have been engineered to contain antibody domains, for example for ease of expression, detection, stability or increased in vivo half-life.
Read more about how secondary antibodies specifically targeted to the CH2 and CH3 domains of immunoglobulins enable the study of Fc fragments in the development of new therapeutic antibody fragments.
3. Analyte Information
IgM
Immunoglobulin M (IgM) is a basic antibody produced
by B cells. IgM is the first antibody to emerge in
response to initial exposure to an antigen. IgM
antibodies are found in the blood and lymph fluid and
are the third most widespread serum Ig.
Immunoglobulin M (IgM), being the 3rd most
widespread serum Ig and exists in two forms- mostly as
a pentamer (970kDa) but also as a hexamer. The
pentameric IgM has 10 binding sites since each
monomer has two antigen binding sites. Due to
distance constraints in the hexameric complex, the J
chain is found in pentameric IgM but not in the
hexameric form. IgM antibodies, which appear early in
the course of an infection, typically reappear to a
smaller extent after additional exposure. IgM, as
opposed to IgG antibodies, do not pass across the
human placenta. These properties of IgM make it
suitable for the diagnosis of infectious diseases.
4. Anti-Human IgM monoclonal antibodies was generated against normal Human
IgM protein and purified from ascites/Culture Supernatant.
Dengue IgM
Positive
5. Test Specification
Appearance Clear colourless liquid
Specificity Specific for the Normal Human IgM
Molecular Weight Observed single band @ ~150kDA under non reducing condition &
two bands of heavy & light chain @ ~50kDA & ~25kDA respectively
under reducing condition.
Purity ≥ 95% by SDS PAGE
Protein Concentration 3-10mg/ml
6. Additional Information
Clone Clone-01/02
Isotype IgG1
Format Liquid
Source Ascites or Culture Supernatant
Immunogen Normal Human IgG
Purification Protein A chromatography
Solvents Compatible with any physiological buffer or those used in
immunodiagnostics methods such as Rapid Diagnostics test, ELISA Dot
blots or western blots
Buffer 0.01M PBS, pH 7.30
Preservative 0.02% Sodium Azide
Cross Reactivity We are not aware of cross reactivity with ant naturally occurring
proteins.
Titer Optimal Coating concentration for ELISA is 250-500ng/well
Each laboratory should determine an optimum working titer for use in its
application.
7. Recommendation Suitable for use in ELISA or Lateral Flow applications. Each laboratory
should determine an optimum working titer for use in its particular
application. Other applications have not been tested but use in such
assays should not necessarily be excluded. Recommended antibody pair
for sandwich immunoassay
Storage Condition Store at 4°C for short term and long-term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Precautions Centrifuge before opening to ensure complete recovery of vial contents.
This product contains sodium azide, which has been classified as Xn
(Harmful), in European Directive 67/548/EEC in the concentration range of
0.1 - 1.0 %. When disposing of this reagent through lead or copper
plumbing, flush with copious volumes of water to prevent azide build-up in
drains.
This product is intended for research and manufacturing uses only. It is not
a diagnostic device
Notes Small volumes of anti-Human IgM antibody vial(s) may occasionally
become entrapped in the seal of the product vial during shipment and
storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to
dislodge any liquid in the container`s cap.
