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Akinrotimi and Aniekwe: Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capiscum annuum L.) in
Abakaliki South-Eastern Agro-Ecological Zone of Nigeria
© 2018, AEXTJ. All Rights Reserved 106
Available Online at www.aextj.com
Agricultural Extension Journal 2018; 2(2):106-110
RESEARCH ARTICLE
Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capsicum
annuum L.) in Abakaliki Southeastern Agro-Ecological Zone of Nigeria
C. A. Akinrotimi1
, N. L. Aniekwe2
1
Department of Agronomy, College of Crop and Soil Science, Michael Okpara University of Agriculture,
Umudike, Abia State, Nigeria, 2
Department of Crop Production and Landscape Management, Faculty of
Agriculture and Natural Resources Management, Ebonyi State University, Abakaliki, Ebonyi State, Nigeria
Received: 25-03-2018; Revised: 01-05-2018; Accepted: 25-05-2018
ABSTRACT
A field experiment was conducted at the research farm of the Department of Crop Production and
Landscape Mangement of the Faculty ofAgriculture, Ebonyi State University,Abakiliki, in Southeasthern
Agro-ecological zone of Nigeria for a period of 8 weeks to study the effect of transplanting age on
growth and yield of green pepper, laid out in a randomized complete block design in four replicates.
The agronomical characteristics that were measured were as follows: Plant height, number of leaves per
plants, days to 50% flowering, number of fruit per plot, and weight of fruit per plot (kg/plot). The result
of the field trial showed that transplanting age had a significant effect only on plant height at 8 weeks
of age of transplanting. The other vegetative parameters such as number of branches, number of leaves,
and 50% flowering and field component such as number and weight of fruits were not affected by the
transplanting age. Therefore, the best age to transplant green pepper is from 1 to 7 weeks, for optimum
performance.
Key words: Crop production, green pepper, Nigeria, transplanting age
INTRODUCTION
Green pepper (Capsicum annuum L.) belongs to
the family Solanaceae which also includes tomato,
tobacco, eggplant, and Irish potato. It is one of
the most varied and widely food condiments in
the world.[1]
Green pepper is originated from
Mexico and Central America regions. Christopher
Columbus encountered pepper in 1493, and because
of its pungent smell, he thought it was related to
black pepper, Piper nigrum, which is actually a
different genus. Nevertheless, the name stuck and
the crop was introduced to Europe, and was later
spread toAsia andAfrica. Many of the early uses of
green pepper centered on medicinal purposes, and
the crop has been credited with a number of useful
cures and treatments, some of which are valid and
some of which are probably more of folklores.[1]
Address for correspondence:
C. A. Akinrotimi,
E-mail: adaakinrotimi@gmail.com
The green pepper is an erect plant with branching
stems which may attain heights of 50–80 cm.
Green peppers are considered a self-pollinating
crop although some outcrossing will occur.
Although it is grown as an annual crop due to its
sensitivity to cold, green pepper is an herbaceous
perennial crop that will survive and yield for
several years in tropical climates. When grown in
deep homogenous soils, it develops a root system
that may extend to a depth of 40 and 70 cm.
Virtually, every country produces green pepper
and its production has increased in recent years
worldwide.[2]
That could be at least in part because
of the high nutritional value of this species. One
green pepper, according to Kelly and Boyhan,[1]
can produce up to 8% of the recommended daily
allowance of Vitamin A, 180% of Vitamin C, 2%
of calcium, and 2% of iron; in addition, pepper
contains significant amount of A and B Vitamins.
In Nigeria, green pepper is one of the vegetable
crops that are grown on large scales in the
northern part of Nigeria. The production of this
crop in Eastern Nigeria is largely in the hands of
ISSN 2521 – 0408
Akinrotimi and Aniekwe: Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capiscum annuum L.) in
Abakaliki South-Eastern Agro-Ecological Zone of Nigeria
AEXTJ/Apr-Jun-2018/Vol 2/Issue 2 107
smallholder farmers who may not be well informed
on some well cultural practices that can promote
production of this crop, such as the appropriate age
of transplanting the seedlings, the right quantity of
fertilizer to apply, and technologies for combating
soil acidity and other fertility problems. Ogbodo
reported that these have resulted in low yield and
exorbitant prices per unit weight of the fruit in the
area.[3]
Transplanting or indirect seedling is the process
of growing seedlings in a greenhouse or other
controlled environment before placing plants
outdoors. The plants are grown in a protected
environment until they are of the right size or
the right weather is optimal and they grow to
maturity.[4]
Conversely, Vantenem and Verlindens
(2003) reported that transplants are not always a
favored method of establishing crops in a field.
This depends on the type of vegetable that is being
planted, the kind of environment the transplants
will be grown and the economic returns that
may be expected from the exercise. The effect of
transplant age on yield is an issue often broached
by growers of agronomic and horticultural crops
in an effort to maximize production potential.
Despite general interest in this area, the literature
is surprisingly sparse.[5]
Agronomic interest in transplant age is most
prominent in rice (Oryza sativa L.), a bare-root
transplanted crop of major economic importance.
Transplantagestudieshavealsobeenconductedon
tobacco Nicotiana tabacum L., cotton Gossypium
hirsutum L., rape Brassica napus L., and forest
species.[6-11]
The largest volume of literature
on transplant age is on vegetables.[12]
Vavrina
suggested ages for field planting for numerous
vegetable transplants.[5]
These recommendations
are generally based on years of horticultural
observations and research. However, the scientific
investigationof vegetabletransplantage is far from
complete. The objectives of this study, therefore,
are to evaluate the effect of various transplanting
ages on the growth and yield of green pepper and
to determine the best transplanting age for this
crop.
MATERIALS AND METHODS
The field experiment was conducted at the research
farm of the Department of Crop Production
and Landscape Management of the Faculty of
Agriculture and Natural Resources management,
EbonyiStateUniversity,Abalikiliki,intheSoutheast
Agro-ecological zone of Nigeria for a period of
18 weeks. The experimental field is located on
the globe at latitude 60
19’407’’N, longitude 80
7’
832”E of the equator, and at an altitude of about
447 m above sea level. The annual rainfall is about
2060 mm spread between April and October with a
slight break in August (August break).
Experimental design and field design
The experiment was laid out in a Randomized
Complete Block Design (RCBD). The treatment
comprised of 7 transplanting ages (1–7 weeks after
germination). The experimental field measured
18 m × 12 m width, which gave a total land area
of 216 m2
. The experimental field was divided into
four equal blocks that were separated by 1 m alley.
Each block consisted of seven plots giving a total
of 28 test plots, each measuring 2 m × 2 m with
0.5 between adjacent plots.
Nursery soil preparation and seedling
establishment
Topsoil samples were collected from the
experimental field of the Faculty of Agriculture
and Natural Resources Management and mixed
with river sand and composted poultry manure
at a ratio of 3:2:1. The mixture was sterilized
using wood fire. Seedboxes were used to raise the
pepper seedling. The nursery was set up on May
17, 2015. The seeds were lightly sprinkled in rows
and lightly covered. Watering of the seed beds and
seedlings were twice daily (morning and evening).
This continued until the end of transplanting
period.
Land preparation and transplanting
The experimental site of land area = 18 m × 12 m
(216 m2
) was manually cleared and raised field
beds of 2 m × 2 m (plot area = 2 m × 2 m 4 m2
).
Space between each block = (1 m2
) and spacing
between plot = (0.5 m) were made on May 14,
2015, for the first transplant. Subsequent beds were
made a day before planting. The seedling were
transplanted into the field beds at 7 transplanting
ages of 1–7 weeks after germination respectively.
The plant spacing of 60 cm × 50 cm was used.
This gave a total of 12 stands per plot.
Akinrotimi and Aniekwe: Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capiscum annuum L.) in
Abakaliki South-Eastern Agro-Ecological Zone of Nigeria
AEXTJ/Apr-Jun-2018/Vol 2/Issue 2 108
Fertilizer application, mulching, weed control,
and harvesting
Basal application of NPK (15:15:15) fertilizer
was applied at 2 weeks after each of the seven
consecutive transplant ages. Rice husk was used as
mulching materials which was spread on each bed
before transplanting. Weeds were controlled using
hoesasoftenasnecessarytokeeptheplotsfreefrom
weeds. Harvesting of the pepper fruits commenced
from the 11th
 week after the first transplant age and
12 weeks after sowing in the nursery, respectively.
This was done manually by hand picking.
Measurement of vegetative and yield components
The plant height of four middle-tagged plants per
plot was measured. The primary (main) stem was
measured from the base of the plant to the apex
2 weeks before the first harvests for the various
transplanting ages using meter rule. The number of
branches produced per four-tagged plants per plot
was counted 2 weeks before the first harvesting of
thefruitsandaveragenumberrecorded.Thenumber
of leaves produced per four middle-tagged plants
per plot was counted before the first harvesting of
the fruits and average number recorded. Days to
50% flowering were recorded at the time when half
of the plant in each plot flowered.
The number of fruits harvested per three middle-
taggedplantsperplotwascountedateachharvestfor
3 times. These were added up and average number
recorded. Fruits from the four middle tagged plants
per plot were weighed at each harvest for 3 times,
using weighing balance. These were added up and
the average fruit weight per plot computed.
Statistical analysis
Statistical analysis of the data collected was done
following the procedure for a RCBD for a two-
way analysis of variance as outlined by Steel and
Torrie.[13]
Separation of treatment means for the
significantdifferencewasdonebytheuseofFisher’s
least significant difference as described by Obi.[14]
RESULTS
Effects of transplanting age on plant height,
number of branches, and leaves
The result as presented in Table 1 showed that
transplanting age had a significant difference
(P  0.05) at 8 weeks after planting. The tallest
plants were produced when the transplants were
1 week in the nursery which was statistically
similar to that of transplanting ages of 2 and 5
weeks. No significant difference existed in all
other weeks after planting. As the number of
weeks after transplanting (WAT) increased from
2 to 8 weeks, the plant height generally increased.
The result in Table 2 showed that transplanting
age had no significant effects on the number of
branches produced at 2, 4, 6, and 8 WAT. However,
the number of branches produced increased from
2 to 8 weeks. The result showed that transplanting
age had no significant effect on the number of
leaves produced at 2, 4, 6, and 8 WAT [Table 3].
However, the number of leaves produced increases
as the week increased from 2 to 8 weeks.
Effect of transplanting age on 50% flowering,
average number of fruits, and average weight
of fruits (kg/plots)
The results in Table 4 indicated that the number of
days required for the 50% of the plant to flower,
was not affected by the transplanting age of the
plant. Furthermore, average number of fruits
and weight of the fruits were not affected by
transplanting age.
DISCUSSION
The result of this field trial showed that
transplanting age had significant effects only on
the plant height produced at 8 WAT [Table 1]. The
vegetative parameters such as number of branches
[Table 2], number of leaves [Table 3], and yield
components such as 50% flowering [Table 4]
number and weight of fruits [Table 4] were not
affected by the transplanting age. This result was
in agreement with the findings of McCraw and
Greig[15]
using 8- and 11-week-old transplants of
fourcultivarsinagreenpeppertransplantagestudy
in Kansas. Pooling the data from the four cultivars,
they found no differences due to transplant age in
early yield (number and weight) in the 1st
 year, but
a greater number of heavier fruits with 8-week-
old transplants the following year. Vavrina and
Armbrester conducted a 1-year trial in Florida
with a transplant of age of 4, 6, and 11 weeks.[16]
They found no effect of transplant age on yield
(number and weight) in three of four harvests, but
a significant yield increases at fourth harvest with
Akinrotimi and Aniekwe: Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capiscum annuum L.) in
Abakaliki South-Eastern Agro-Ecological Zone of Nigeria
AEXTJ/Apr-Jun-2018/Vol 2/Issue 2 109
4-week-old transplants. The yield effect here was
due to a greater number of fruits rather than due
to greater individual fruit weight. McCraw and
Greig[15]
observed a similar finding with 11-week-
old transplants. Three of the studies cited here
imply that pepper transplant of 8–11 weeks may
have a yield advantage for early, size, and number
of fruits[15-17]
and were under quite different
environments, which makes comparisons among
the studies difficult. Perhaps, a standardization of
the number of harvests for early and total yield
is necessary to critically determine the impact of
transplant age on green pepper production.
The result of this experiment was studied under
seven different transplanting ages such as 1–7 weeks
which was carried out at research farm of the
Department of Crop Production and Landscape
Management, Ebonyi State University, Abakiliki.
In general, rice husk was used as crop production
measures to mulch the bed so as to ensure that
total moisture is maintained to enable the plant to
survive and to as to avoid drying off of the leaves.
This was found to be highly effective in moisture
conservation. Transplanting green pepper from 1 to
3 weeks was suitable transplanting time, because
during this stage, the plant can easily withstand
shock due to transplanting, without shedding its leaf.
Green pepper transplanting at these ages thrives
faster more than the ones transplanted from 4 to
7 weeks. Green pepper transplanted from 4 to
7 weeks can hardly withstand shock, and if care
is not taken, all the leaves will completely drop
off, especially the ones at 6 and 7 weeks. At this
age, the plant may have already started flowering
and when transplanted to the field will shed its
leaves and its flowers may abort. Hereby making
it impossible for the green pepper plant to fruit
well and at times, it will be stunted. In this work,
there is no great difference between green pepper
transplanting ages except on plant height produced
at 8 WAT. The other vegetative parameters were
not affected by transplanting age.
Table 1: Effect of transplanting age on plant height (cm)
at 2, 4, 6, and 8 WAT
Transplanting
age (weeks)
Weeks after planting
Week 2 Week 4 Week 6 Week 8
1 8.39 14.00 18.96 21.72
2 8.73 11.29 17.21 23.66
3 8.17 9.83 14.33 21.27
4 8.01 10.84 13.50 20.79
5 7.72 11.21 15.97 23.29
6 7.63 7.62 14.88 20.26
7 7.74 8.12 13.38 20.74
Mean 8.06 10.42 15.46 22.10
F‑LSD (0.05) ns ns ns 2.36
Key: NS: Not significant. F‑LSD: Fisher’s least significant difference,
WAT: Weeks after transplanting
Table 2: Effect of transplanting age on number of
branches at 2, 4, 6, and 8 WAT
Transplanting
age (weeks)
Weeks after planting
Week 2 Week 4 Week 6 Week 8
1 2.17 2.76 4.00 4.25
2 2.18 2.42 3.57 5.25
3 2.33 2.57 3.00 4.25
4 2.08 2.33 4.00 4.00
5 1.92 2.74 3.50 4.25
6 2.25 2.43 3.82 3.57
7 2.08 2.42 3.57 4.00
Mean 2.23 2.44 3.64 4.22
F‑LSD (0.05) ns ns ns ns
Key: NS: Not significant. F‑LSD: Fisher’s least significant difference,
WAT: Weeks after transplanting
Table 3: Effect of transplanting on number of branches at
2, 4, 6, and 8 WAT
Transplanting
age (weeks)
Weeks after planting
Week 2 Week 4 Week 6 Week 8
1 8.17 12.17 23.91 34.25
2 6.83 12.50 22.92 33.33
3 8.08 12.00 24.08 34.00
4 8.50 12.09 23.50 31.50
5 6.92 11.00 22.00 33.42
6 7.25 10.25 22.25 34.42
7 7.75 11.00 24.11 34.88
Mean 7.64 11.57 23.25 33.89
F‑LSD (0.05) ns ns ns ns
Key: NS: Not significant. F‑LSD: Fisher’s least significant difference,
WAT: Weeks after transplanting
Table 4: Effects of transplanting age days to 50%
flowering, average number, and weight of fruits/plot
Transplanting
age (weeks)
Days
to 50%
flowering
Average
number of
fruits/plot
Average
weight of
fruits (kg)/plot
1 75.8 7.25 0.450
2 74.2 6.75 0.500
3 78.2 6.50 0.525
4 71.8 7.00 0.560
5 81.5 6.25 0.685
6 82.8 6.50 0.475
7 74.8 6.00 0.475
Mean 77.00 6.61 0.531
F‑LSD (0.05) ns ns ns
Key: NS: Not significant. F‑LSD: Fisher’s least significant difference
Akinrotimi and Aniekwe: Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capiscum annuum L.) in
Abakaliki South-Eastern Agro-Ecological Zone of Nigeria
AEXTJ/Apr-Jun-2018/Vol 2/Issue 2 110
CONCLUSIONS AND
RECOMMENDATIONS
Since transplanting age had significant effect
on plant weight only, I therefore recommend
that farmers should use Green pepper transplant
of 1–7 weeks old for planting on their farms to
ensure adequate yield so as to enhance improved
production because transplanting of green pepper
from the 1–7 weeks from the nursery farm to its
permanent place on the field will make the green
pepper to withstand shock and also there will be
no leaves, thereby increasing its productivity.
REFERENCES
1.	 Kelly WT, Boyhan G. Pepper History, Scope, Climate
and Taxonomy. In: Commercial Pepper Production
Handbook. University of Georgia, Athens, USA; 2009.
2.	 Ekwu LG, Utobo EB. Response of pepper (Capiscaum
annum L.) to phosphorus fertilizer application and
transplanting age. J Appl Sci 2008;11:7814-26.
3.	 Ogbodo EN. Changes in the properties of an Acid Soil
Amended with Burnt Rice Husk and the Effect on the
Growth and Yield of Pepper atAbakiliki South Easthern
Nigeria. Am Eur J Sustain Agric 2009;3:579-86.
4.	 Chiesa A, Yapoudjian A, Clantiffe DJ. Influence
of planting age on tomato species. Horticultural
2004;256:331-50.
5.	 Vavrina CS. An introduction to the Production of
containerized vegetable transplant. Univ Fla Coop Ext
Serv Bull 1998;302:1-32.
6.	 Abou-Zeid HM, Abd-ElAal HA, Darwish AA, EI-
Shazly WM. Transplanting techniques and seedlings
age influence on agronomic performance of Giza 75
cotton cultivar r. Ann J Agric Sci 1995;40:609-19.
7.	 Chaney WR, Byrnes WR. Effects of seedling age and
taproot length on performance of oark. Appl Forestry
Res 1993;4:175-8.
8.	 Greenfield PL, Paterson BJ. Production of uniform
tobacco seedlings in trays. V. Influence of cell size
and seedling age on uniformity of plants in the field in
comparison with seedbed transplants. Appl Plant Sci
1994;8:6-9.
9.	 Gupta TR. Effects of transplanting on gobhi sarson
Brassica napus. Oleifera varnaptus. Indian J Argron
1994;39:146-7.
10.	 Sheriff MN, Selim MM, Kamel AS. Studies on some
factors affecting seed and fibre properties of transplanted
cotton. Ann J Agric Sci (Egyptian) 1995;33:647-57.
11.	 Tancogne J. Possibility of prolonging the storage period
on tobacco plants produced in miniblocks. Ann Du
Tobac (French) 1991;23:89-93.
12.	 Lorenze OA, Maynard DN. Knotts Handbook for
Vegetable Growers. 3rd
 ed. New York: Wiley; 1988.
13.	 Steel GD, Torrie JH. Principles and Procedures of
Statistics: A Biometrical Approach. 2nd
 ed. New York:
McGraw-Hill Book Company Inc.; 1980. p. 633.
14.	 Obi IU. Statistical Methods of Detecting Differences
Between Treatment Means and Research Methodology
Issues in the Laboratory and Field Experiments. 2nd
 ed.
Enugu: SNAAP Press Nigeria Limited; 2002. p. 97.
15.	 McCraw BD, Greig JK. Effect of transplant age and
pruning procedure on yield and fruit-set of bell pepper.
Hortic Sci 2006;21:430-1.
16.	 Vavrina CS, Armbrester K. Effects of transplant age and
cell size on pepper production. SWFREC Res Rpt IMM
2004;12:91-8.
17.	 Weston LA. Effect of flat cell size, transplant age
and production site on growth and yield of pepper
transplants. Hortic Sci 2008;22:709-11.

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Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capsicum annuum L.) in Abakaliki Southeastern Agro-Ecological Zone of Nigeria

  • 1. Akinrotimi and Aniekwe: Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capiscum annuum L.) in Abakaliki South-Eastern Agro-Ecological Zone of Nigeria © 2018, AEXTJ. All Rights Reserved 106 Available Online at www.aextj.com Agricultural Extension Journal 2018; 2(2):106-110 RESEARCH ARTICLE Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capsicum annuum L.) in Abakaliki Southeastern Agro-Ecological Zone of Nigeria C. A. Akinrotimi1 , N. L. Aniekwe2 1 Department of Agronomy, College of Crop and Soil Science, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria, 2 Department of Crop Production and Landscape Management, Faculty of Agriculture and Natural Resources Management, Ebonyi State University, Abakaliki, Ebonyi State, Nigeria Received: 25-03-2018; Revised: 01-05-2018; Accepted: 25-05-2018 ABSTRACT A field experiment was conducted at the research farm of the Department of Crop Production and Landscape Mangement of the Faculty ofAgriculture, Ebonyi State University,Abakiliki, in Southeasthern Agro-ecological zone of Nigeria for a period of 8 weeks to study the effect of transplanting age on growth and yield of green pepper, laid out in a randomized complete block design in four replicates. The agronomical characteristics that were measured were as follows: Plant height, number of leaves per plants, days to 50% flowering, number of fruit per plot, and weight of fruit per plot (kg/plot). The result of the field trial showed that transplanting age had a significant effect only on plant height at 8 weeks of age of transplanting. The other vegetative parameters such as number of branches, number of leaves, and 50% flowering and field component such as number and weight of fruits were not affected by the transplanting age. Therefore, the best age to transplant green pepper is from 1 to 7 weeks, for optimum performance. Key words: Crop production, green pepper, Nigeria, transplanting age INTRODUCTION Green pepper (Capsicum annuum L.) belongs to the family Solanaceae which also includes tomato, tobacco, eggplant, and Irish potato. It is one of the most varied and widely food condiments in the world.[1] Green pepper is originated from Mexico and Central America regions. Christopher Columbus encountered pepper in 1493, and because of its pungent smell, he thought it was related to black pepper, Piper nigrum, which is actually a different genus. Nevertheless, the name stuck and the crop was introduced to Europe, and was later spread toAsia andAfrica. Many of the early uses of green pepper centered on medicinal purposes, and the crop has been credited with a number of useful cures and treatments, some of which are valid and some of which are probably more of folklores.[1] Address for correspondence: C. A. Akinrotimi, E-mail: adaakinrotimi@gmail.com The green pepper is an erect plant with branching stems which may attain heights of 50–80 cm. Green peppers are considered a self-pollinating crop although some outcrossing will occur. Although it is grown as an annual crop due to its sensitivity to cold, green pepper is an herbaceous perennial crop that will survive and yield for several years in tropical climates. When grown in deep homogenous soils, it develops a root system that may extend to a depth of 40 and 70 cm. Virtually, every country produces green pepper and its production has increased in recent years worldwide.[2] That could be at least in part because of the high nutritional value of this species. One green pepper, according to Kelly and Boyhan,[1] can produce up to 8% of the recommended daily allowance of Vitamin A, 180% of Vitamin C, 2% of calcium, and 2% of iron; in addition, pepper contains significant amount of A and B Vitamins. In Nigeria, green pepper is one of the vegetable crops that are grown on large scales in the northern part of Nigeria. The production of this crop in Eastern Nigeria is largely in the hands of ISSN 2521 – 0408
  • 2. Akinrotimi and Aniekwe: Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capiscum annuum L.) in Abakaliki South-Eastern Agro-Ecological Zone of Nigeria AEXTJ/Apr-Jun-2018/Vol 2/Issue 2 107 smallholder farmers who may not be well informed on some well cultural practices that can promote production of this crop, such as the appropriate age of transplanting the seedlings, the right quantity of fertilizer to apply, and technologies for combating soil acidity and other fertility problems. Ogbodo reported that these have resulted in low yield and exorbitant prices per unit weight of the fruit in the area.[3] Transplanting or indirect seedling is the process of growing seedlings in a greenhouse or other controlled environment before placing plants outdoors. The plants are grown in a protected environment until they are of the right size or the right weather is optimal and they grow to maturity.[4] Conversely, Vantenem and Verlindens (2003) reported that transplants are not always a favored method of establishing crops in a field. This depends on the type of vegetable that is being planted, the kind of environment the transplants will be grown and the economic returns that may be expected from the exercise. The effect of transplant age on yield is an issue often broached by growers of agronomic and horticultural crops in an effort to maximize production potential. Despite general interest in this area, the literature is surprisingly sparse.[5] Agronomic interest in transplant age is most prominent in rice (Oryza sativa L.), a bare-root transplanted crop of major economic importance. Transplantagestudieshavealsobeenconductedon tobacco Nicotiana tabacum L., cotton Gossypium hirsutum L., rape Brassica napus L., and forest species.[6-11] The largest volume of literature on transplant age is on vegetables.[12] Vavrina suggested ages for field planting for numerous vegetable transplants.[5] These recommendations are generally based on years of horticultural observations and research. However, the scientific investigationof vegetabletransplantage is far from complete. The objectives of this study, therefore, are to evaluate the effect of various transplanting ages on the growth and yield of green pepper and to determine the best transplanting age for this crop. MATERIALS AND METHODS The field experiment was conducted at the research farm of the Department of Crop Production and Landscape Management of the Faculty of Agriculture and Natural Resources management, EbonyiStateUniversity,Abalikiliki,intheSoutheast Agro-ecological zone of Nigeria for a period of 18 weeks. The experimental field is located on the globe at latitude 60 19’407’’N, longitude 80 7’ 832”E of the equator, and at an altitude of about 447 m above sea level. The annual rainfall is about 2060 mm spread between April and October with a slight break in August (August break). Experimental design and field design The experiment was laid out in a Randomized Complete Block Design (RCBD). The treatment comprised of 7 transplanting ages (1–7 weeks after germination). The experimental field measured 18 m × 12 m width, which gave a total land area of 216 m2 . The experimental field was divided into four equal blocks that were separated by 1 m alley. Each block consisted of seven plots giving a total of 28 test plots, each measuring 2 m × 2 m with 0.5 between adjacent plots. Nursery soil preparation and seedling establishment Topsoil samples were collected from the experimental field of the Faculty of Agriculture and Natural Resources Management and mixed with river sand and composted poultry manure at a ratio of 3:2:1. The mixture was sterilized using wood fire. Seedboxes were used to raise the pepper seedling. The nursery was set up on May 17, 2015. The seeds were lightly sprinkled in rows and lightly covered. Watering of the seed beds and seedlings were twice daily (morning and evening). This continued until the end of transplanting period. Land preparation and transplanting The experimental site of land area = 18 m × 12 m (216 m2 ) was manually cleared and raised field beds of 2 m × 2 m (plot area = 2 m × 2 m 4 m2 ). Space between each block = (1 m2 ) and spacing between plot = (0.5 m) were made on May 14, 2015, for the first transplant. Subsequent beds were made a day before planting. The seedling were transplanted into the field beds at 7 transplanting ages of 1–7 weeks after germination respectively. The plant spacing of 60 cm × 50 cm was used. This gave a total of 12 stands per plot.
  • 3. Akinrotimi and Aniekwe: Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capiscum annuum L.) in Abakaliki South-Eastern Agro-Ecological Zone of Nigeria AEXTJ/Apr-Jun-2018/Vol 2/Issue 2 108 Fertilizer application, mulching, weed control, and harvesting Basal application of NPK (15:15:15) fertilizer was applied at 2 weeks after each of the seven consecutive transplant ages. Rice husk was used as mulching materials which was spread on each bed before transplanting. Weeds were controlled using hoesasoftenasnecessarytokeeptheplotsfreefrom weeds. Harvesting of the pepper fruits commenced from the 11th  week after the first transplant age and 12 weeks after sowing in the nursery, respectively. This was done manually by hand picking. Measurement of vegetative and yield components The plant height of four middle-tagged plants per plot was measured. The primary (main) stem was measured from the base of the plant to the apex 2 weeks before the first harvests for the various transplanting ages using meter rule. The number of branches produced per four-tagged plants per plot was counted 2 weeks before the first harvesting of thefruitsandaveragenumberrecorded.Thenumber of leaves produced per four middle-tagged plants per plot was counted before the first harvesting of the fruits and average number recorded. Days to 50% flowering were recorded at the time when half of the plant in each plot flowered. The number of fruits harvested per three middle- taggedplantsperplotwascountedateachharvestfor 3 times. These were added up and average number recorded. Fruits from the four middle tagged plants per plot were weighed at each harvest for 3 times, using weighing balance. These were added up and the average fruit weight per plot computed. Statistical analysis Statistical analysis of the data collected was done following the procedure for a RCBD for a two- way analysis of variance as outlined by Steel and Torrie.[13] Separation of treatment means for the significantdifferencewasdonebytheuseofFisher’s least significant difference as described by Obi.[14] RESULTS Effects of transplanting age on plant height, number of branches, and leaves The result as presented in Table 1 showed that transplanting age had a significant difference (P 0.05) at 8 weeks after planting. The tallest plants were produced when the transplants were 1 week in the nursery which was statistically similar to that of transplanting ages of 2 and 5 weeks. No significant difference existed in all other weeks after planting. As the number of weeks after transplanting (WAT) increased from 2 to 8 weeks, the plant height generally increased. The result in Table 2 showed that transplanting age had no significant effects on the number of branches produced at 2, 4, 6, and 8 WAT. However, the number of branches produced increased from 2 to 8 weeks. The result showed that transplanting age had no significant effect on the number of leaves produced at 2, 4, 6, and 8 WAT [Table 3]. However, the number of leaves produced increases as the week increased from 2 to 8 weeks. Effect of transplanting age on 50% flowering, average number of fruits, and average weight of fruits (kg/plots) The results in Table 4 indicated that the number of days required for the 50% of the plant to flower, was not affected by the transplanting age of the plant. Furthermore, average number of fruits and weight of the fruits were not affected by transplanting age. DISCUSSION The result of this field trial showed that transplanting age had significant effects only on the plant height produced at 8 WAT [Table 1]. The vegetative parameters such as number of branches [Table 2], number of leaves [Table 3], and yield components such as 50% flowering [Table 4] number and weight of fruits [Table 4] were not affected by the transplanting age. This result was in agreement with the findings of McCraw and Greig[15] using 8- and 11-week-old transplants of fourcultivarsinagreenpeppertransplantagestudy in Kansas. Pooling the data from the four cultivars, they found no differences due to transplant age in early yield (number and weight) in the 1st  year, but a greater number of heavier fruits with 8-week- old transplants the following year. Vavrina and Armbrester conducted a 1-year trial in Florida with a transplant of age of 4, 6, and 11 weeks.[16] They found no effect of transplant age on yield (number and weight) in three of four harvests, but a significant yield increases at fourth harvest with
  • 4. Akinrotimi and Aniekwe: Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capiscum annuum L.) in Abakaliki South-Eastern Agro-Ecological Zone of Nigeria AEXTJ/Apr-Jun-2018/Vol 2/Issue 2 109 4-week-old transplants. The yield effect here was due to a greater number of fruits rather than due to greater individual fruit weight. McCraw and Greig[15] observed a similar finding with 11-week- old transplants. Three of the studies cited here imply that pepper transplant of 8–11 weeks may have a yield advantage for early, size, and number of fruits[15-17] and were under quite different environments, which makes comparisons among the studies difficult. Perhaps, a standardization of the number of harvests for early and total yield is necessary to critically determine the impact of transplant age on green pepper production. The result of this experiment was studied under seven different transplanting ages such as 1–7 weeks which was carried out at research farm of the Department of Crop Production and Landscape Management, Ebonyi State University, Abakiliki. In general, rice husk was used as crop production measures to mulch the bed so as to ensure that total moisture is maintained to enable the plant to survive and to as to avoid drying off of the leaves. This was found to be highly effective in moisture conservation. Transplanting green pepper from 1 to 3 weeks was suitable transplanting time, because during this stage, the plant can easily withstand shock due to transplanting, without shedding its leaf. Green pepper transplanting at these ages thrives faster more than the ones transplanted from 4 to 7 weeks. Green pepper transplanted from 4 to 7 weeks can hardly withstand shock, and if care is not taken, all the leaves will completely drop off, especially the ones at 6 and 7 weeks. At this age, the plant may have already started flowering and when transplanted to the field will shed its leaves and its flowers may abort. Hereby making it impossible for the green pepper plant to fruit well and at times, it will be stunted. In this work, there is no great difference between green pepper transplanting ages except on plant height produced at 8 WAT. The other vegetative parameters were not affected by transplanting age. Table 1: Effect of transplanting age on plant height (cm) at 2, 4, 6, and 8 WAT Transplanting age (weeks) Weeks after planting Week 2 Week 4 Week 6 Week 8 1 8.39 14.00 18.96 21.72 2 8.73 11.29 17.21 23.66 3 8.17 9.83 14.33 21.27 4 8.01 10.84 13.50 20.79 5 7.72 11.21 15.97 23.29 6 7.63 7.62 14.88 20.26 7 7.74 8.12 13.38 20.74 Mean 8.06 10.42 15.46 22.10 F‑LSD (0.05) ns ns ns 2.36 Key: NS: Not significant. F‑LSD: Fisher’s least significant difference, WAT: Weeks after transplanting Table 2: Effect of transplanting age on number of branches at 2, 4, 6, and 8 WAT Transplanting age (weeks) Weeks after planting Week 2 Week 4 Week 6 Week 8 1 2.17 2.76 4.00 4.25 2 2.18 2.42 3.57 5.25 3 2.33 2.57 3.00 4.25 4 2.08 2.33 4.00 4.00 5 1.92 2.74 3.50 4.25 6 2.25 2.43 3.82 3.57 7 2.08 2.42 3.57 4.00 Mean 2.23 2.44 3.64 4.22 F‑LSD (0.05) ns ns ns ns Key: NS: Not significant. F‑LSD: Fisher’s least significant difference, WAT: Weeks after transplanting Table 3: Effect of transplanting on number of branches at 2, 4, 6, and 8 WAT Transplanting age (weeks) Weeks after planting Week 2 Week 4 Week 6 Week 8 1 8.17 12.17 23.91 34.25 2 6.83 12.50 22.92 33.33 3 8.08 12.00 24.08 34.00 4 8.50 12.09 23.50 31.50 5 6.92 11.00 22.00 33.42 6 7.25 10.25 22.25 34.42 7 7.75 11.00 24.11 34.88 Mean 7.64 11.57 23.25 33.89 F‑LSD (0.05) ns ns ns ns Key: NS: Not significant. F‑LSD: Fisher’s least significant difference, WAT: Weeks after transplanting Table 4: Effects of transplanting age days to 50% flowering, average number, and weight of fruits/plot Transplanting age (weeks) Days to 50% flowering Average number of fruits/plot Average weight of fruits (kg)/plot 1 75.8 7.25 0.450 2 74.2 6.75 0.500 3 78.2 6.50 0.525 4 71.8 7.00 0.560 5 81.5 6.25 0.685 6 82.8 6.50 0.475 7 74.8 6.00 0.475 Mean 77.00 6.61 0.531 F‑LSD (0.05) ns ns ns Key: NS: Not significant. F‑LSD: Fisher’s least significant difference
  • 5. Akinrotimi and Aniekwe: Effect of Transplanting Age on the Growth and Yield of Green Pepper (Capiscum annuum L.) in Abakaliki South-Eastern Agro-Ecological Zone of Nigeria AEXTJ/Apr-Jun-2018/Vol 2/Issue 2 110 CONCLUSIONS AND RECOMMENDATIONS Since transplanting age had significant effect on plant weight only, I therefore recommend that farmers should use Green pepper transplant of 1–7 weeks old for planting on their farms to ensure adequate yield so as to enhance improved production because transplanting of green pepper from the 1–7 weeks from the nursery farm to its permanent place on the field will make the green pepper to withstand shock and also there will be no leaves, thereby increasing its productivity. REFERENCES 1. Kelly WT, Boyhan G. Pepper History, Scope, Climate and Taxonomy. In: Commercial Pepper Production Handbook. University of Georgia, Athens, USA; 2009. 2. Ekwu LG, Utobo EB. Response of pepper (Capiscaum annum L.) to phosphorus fertilizer application and transplanting age. J Appl Sci 2008;11:7814-26. 3. Ogbodo EN. Changes in the properties of an Acid Soil Amended with Burnt Rice Husk and the Effect on the Growth and Yield of Pepper atAbakiliki South Easthern Nigeria. Am Eur J Sustain Agric 2009;3:579-86. 4. Chiesa A, Yapoudjian A, Clantiffe DJ. Influence of planting age on tomato species. Horticultural 2004;256:331-50. 5. Vavrina CS. An introduction to the Production of containerized vegetable transplant. Univ Fla Coop Ext Serv Bull 1998;302:1-32. 6. Abou-Zeid HM, Abd-ElAal HA, Darwish AA, EI- Shazly WM. Transplanting techniques and seedlings age influence on agronomic performance of Giza 75 cotton cultivar r. Ann J Agric Sci 1995;40:609-19. 7. Chaney WR, Byrnes WR. Effects of seedling age and taproot length on performance of oark. Appl Forestry Res 1993;4:175-8. 8. Greenfield PL, Paterson BJ. Production of uniform tobacco seedlings in trays. V. Influence of cell size and seedling age on uniformity of plants in the field in comparison with seedbed transplants. Appl Plant Sci 1994;8:6-9. 9. Gupta TR. Effects of transplanting on gobhi sarson Brassica napus. Oleifera varnaptus. Indian J Argron 1994;39:146-7. 10. Sheriff MN, Selim MM, Kamel AS. Studies on some factors affecting seed and fibre properties of transplanted cotton. Ann J Agric Sci (Egyptian) 1995;33:647-57. 11. Tancogne J. Possibility of prolonging the storage period on tobacco plants produced in miniblocks. Ann Du Tobac (French) 1991;23:89-93. 12. Lorenze OA, Maynard DN. Knotts Handbook for Vegetable Growers. 3rd  ed. New York: Wiley; 1988. 13. Steel GD, Torrie JH. Principles and Procedures of Statistics: A Biometrical Approach. 2nd  ed. New York: McGraw-Hill Book Company Inc.; 1980. p. 633. 14. Obi IU. Statistical Methods of Detecting Differences Between Treatment Means and Research Methodology Issues in the Laboratory and Field Experiments. 2nd  ed. Enugu: SNAAP Press Nigeria Limited; 2002. p. 97. 15. McCraw BD, Greig JK. Effect of transplant age and pruning procedure on yield and fruit-set of bell pepper. Hortic Sci 2006;21:430-1. 16. Vavrina CS, Armbrester K. Effects of transplant age and cell size on pepper production. SWFREC Res Rpt IMM 2004;12:91-8. 17. Weston LA. Effect of flat cell size, transplant age and production site on growth and yield of pepper transplants. Hortic Sci 2008;22:709-11.