collection of urine specimens

1. Collection and transport of urine
specimen
Arman ali
M.Sc final year

2. INTRODUCTION
 Collection Techniques :- Specimens should be collected during the acute
(early) phase of an illness (or within 2 to 3 days for viral infections) and
before antimicrobials, antifungals, or antiviral medications are
administered.
 Patient information to match & lavel properly :-
1. Name
2. Identification number
3. Collection Information
4. Date and time
5. Total volume
6. Collection duration (i.e., 24-hour)

3. Information For The Nursing Staff And
Clinicians Should Include The Following
 Safety considerations
 Selection of the appropriate anatomical site and specimen
 Collection instructions, including the type of swab or transport
medium
 Transportation instructions, including time and temperature
constraints
 Labeling instructions, including patient demographic information
(minimum of two patient identifiers)
 Special instructions, such as patient preparation Sterile versus
nonsterile- collection devices

4. URINE SPECIMEN
 Clean- voided midstream (CVS)
 Container :-
Sterile, screwcap container ; Containers that include a variety of chemical
urinalysis preservatives may also be used.
 Patient Preparation :-
 Females: clean area with soap and water, then rinse with water; hold
labia apart and begin voiding in commode; after several mL have
passed, collect midstream.
 Males: clean glans with soap and water, then rinse with water;
retract foreskin; begin voiding in commode; after several mL have
passed, collect midstream.

5. From Straight catheter:
 container
Sterile, screw-cap container or urine transport tube with boric acid
preservative
 Patient Preparation
In catheterized patients , urine should be collected from the catheter tube
(after disinfecting ) Insert catheter into bladder; allow first 15 mL to
pass; then collect remain.
 Special Instructions – urine not collect from uro bag.
 Transportation to Laboratory
Unpreserved ≤1/2 h/RT- Preserved ≤ 24 h/RT ; Storage Before Processing
24 h/4°C

7. From Suprapubic aspiratation:
 Suprapubic aspiration (SPA) is a sterile procedure that allows for the sampling of
uncontaminated urine in patients.
 It is considered the gold standard for collecting urine for urinalysis in children
 it is recommended for patients in coma or infants.
 container - Sterile, screwcap container or anaerobic transporter
 Patient Preparation -Disinfect skin.
 Special Instructions
Needle aspiration above the symphysis pubis through the abdominal wall into the full
bladder.
 Storage-Immediately/RT

8. PROCEDURE
• Insert the needle (attached to the
syringe) perpendicular to the skin at
1-2 cm superior to the pubic
symphysis (the suprapubic crease
level) in the midline:
• Aspirate gently after the needle
pierces the skin.
• Aspirate as you insert, as well as
when you withdraw the needle.

9. Thank you