Materi Kuliah Pengelolaan Tanah Fakultas Pertanian UGM

1. INOVASI TEKNOLOGI DI FAKULTAS PERTANIAN UGM
DALAM MENDUKUNG PROGRAM UPSUS PAJALE
TIM UPSUS PAJALE
FAKULTAS PERTANIAN, UNIVERSITAS GADJAH MADA
2015
jwidada@ugm.ac.id

2. KESEJAHTERAAN PETANI
SWASEMBADA KEBERLANJUTAN
EKOLOGI
PRODUKSI
KEDELAI

3. Ecologi yang Berkelanjutan dalam Produksi
PANGAN

4. Komposisi Mikrobiom pada Rizosfer akan Menentukan
Fenotipik Tanaman dan Proses-Proses dalam Tanah
Plant Physiol. Vol. 166, 2014

5. Kehidupan mikrobiom pada Padi
The plant habitat for microbial life
!Rhizosphere
!Endosphere
! Phyllosphere
The plant habitat for microbial life
!Rhizosphere
!Endosphere
! Phyllosphere

6. Key functional potential of
(uncultured) rice endophytes
! Colonization (Flagella, pili, outer membrane
systems, cellulases, pectinases, xylanases,
secretion systems…)
! Life inside the plant (uptake of aa, sugars,
aromatic degradation, CH4 oxidation, ability to
cope with oxidative stress, N cycling (N-fixation,
nitrification and denitrification), PHA synthesis/
degradation, quorum sensing…)
! Plant nutrition (N-fixation, siderophores)
! Improvement of plant stress tolerance
(ACC deaminase, ability to cope with oxidative
stress, detoxification)
! Plant growth promotion (ACC deaminase,
siderophores, IAA, antibiotics, N fixation…)
Sessitsch et al, 2012. Mol.Plant-Microbe Interact.
Key functional potential of
(uncultured) rice endophytes
! Colonization (Flagella, pili, outer membrane
systems, cellulases, pectinases, xylanases,
secretion systems…)
! Life inside the plant (uptake of aa, sugars,
aromatic degradation, CH4 oxidation, ability to
cope with oxidative stress, N cycling (N-fixation,
nitrification and denitrification), PHA synthesis/
degradation, quorum sensing…)
! Plant nutrition (N-fixation, siderophores)
! Improvement of plant stress tolerance
(ACC deaminase, ability to cope with oxidative
stress, detoxification)
! Plant growth promotion (ACC deaminase,
siderophores, IAA, antibiotics, N fixation…)
Sessitsch et al, 2012. Mol.Plant-Microbe Interact.
Peran Kunci Endofitik Padi

7. Pengayaan Bakteri Endofitik Padi
12 B. Lugtenberg
Isolation root tip
After 3 Cycles
Strains acting through CNN
Inoculate seed or seedlings
Growth in quartz
sand/nutrient solution
Vortex and dilute on KB plate
Judge colony diversity
Growth o/n
Collect all colonies
Suspension of rhizosphere
bacteria
Fig. 3.2 Enrichment of bacteria which compete efficiently for nutrients and niches. Starting from

8. Identifikasi Endofitik Padi
G
8
Burkholderia group

9. Kemampuan Endofitik Padi dalam Pelarutan
Fosfat dan Pemacuan Pertumbuhan Tanaman
C ONT +PSB
s s
C ONT +PSB
Rock Phosphate Super Phosphate
Cont.: Uninoculated
+PSB: inoculated with PSB
Burkholderia sp. G8

10. Efek Endofitik Padi pada Ketahanan Padi
Terhadap Virus Kerdil

11. Pendampingan Kelompok Tani
(Pembuatan BACILLUS PLUS Secara Mandiri)

12. Penyiapan BACILLUS PLUS
di Kelompok Tani

13. Bacillus PlusKontrol
Inokulasi BACILLUS PLUS
pada Pembibitan Padi

14. Pembibitan Padi Sehat dalam Kelambu Secara Basah
di Lahan dan Secara Kering dalam Rak

15. Penggunaan BACILLUS PLUS pada
Pembibitan Padi di Lahan Petani

16. Efek Aplikasi BACILLUS PLUS
pada Pertanaman Padi di Moyudan Sleman

17. Efek Aplikasi BACILLUS PLUS
pada Pertanaman Padi di Imogiri Bantul

18. Denfarm Di Sragen
(Aplikasi BACILLUS PLUS)

19. Efek Aplikasi BACILLUS PLUS
pada Padi di Moyudan Sleman
K B

20. Kombinasi BACILLUS PLUS dan Teknologi
Ramah Lingkungan

21. Pengembangan Teknologi Rhizobium
untuk Kedelai (LEGIN+)
+

22. Schematic view of the mycorrhizosphere concept in contrast to the rhizosphere concept:
Features of conventionally managed agricultural soils versus sustainably managed
agricultural soils are indicated with emphasis on mycorrhizosphere components and
possible effects on them.
Jonas F. Johansson et al. FEMS Microbiol Ecol 2004;48:1-
13
© 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights
reserved.

23. Pengembangan Teknologi Rhizobium
untuk Kedelai (LEGIN+)

24. •Glomus manihotis strain M1
M0: without mycorrhizal fungi; M1: inoculated with mycorrhozal fungi
SOYBEANCORN
Strain M1
Pengembangan Teknologi Mikoriza untuk
Kedelai & Jagung

26. Uji Potensi Teknologi Mikoriza

27. Respon 18 kultivar
kedeali terhadap
inokulasi mikoriza

28. Perlakuan
benih dg
Mikoriza
Kontrol
Pengaruh Mikoriza terhadap Pertumbuhan Kedelai

29. Pengaruh Mikoriza terhadap Hasil Panen
Kedelai
Perlakuan
benih dg
Mikoriza
Kontrol

30. Sistem Tumpang Sari di Pacitan JATIM

31. Pengujian Teknologi Mikoriza
di Lahan Bernaungan

32. Pendampingan Petani di Lahan Marginal
(Teknologi Mikoriza)

33. Pendapingan di Nglipar Gn. Kidul

34. Uji Teknologi Mikoriza & Legin
di Nglipar Gn. Kidul
Varietas ARGOSEWU ( Kethek
Putih) Umur Panen 70 hari

35. Pendampingan Petani di Pundong
Bantul

36. Aplikasi Mikoriza di Playen Gn Kidul

37. Pendampingan Petani di Playen Gn. Kidul

38. Persentase Kemelimpahan Mikoriza Arbuskular pada
Berbagai Kultivar Jagung
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
Unyil Super Unyil Kuning Bima Srikandi Lamuru Bisma
PersentaseKelimpahan
(%)
Kultivar Jagung Lokal
Glomus sp.
unidentified
Scutellospora sp.
Acaulospora sp.
Gigaspora sp.

39. Aplikasi Mikoriza pada Jagung di Blora

40. -Zn-P -Cu
Aplikasi Mikoriza pada Jagung akan
Meningkatkan Hasil Panen karena Tanaman
kecukupan P, Zn dan Cu

41. Terima Kasih

43. Implementation of plant-growth promoting traits
in PGPR functional groups
Vacheron et al., 2013

44. Induce systemic resistance by PGPR