Morchella, the true morels, belonging to Helvellaceae family of class Ascomycetes, are amongst the most highly prized fungi in the world. Their artificial production is still a challenge, even though patents for their cultivation do exist. The tissue of Morchella sp. was transferred aseptically to Potato Dextrose Agar medium (peeled, sliced and boiled potato, 200 g; dextrose, 20 g; agar, 20 g L-1) to grow hyphae. The mycelium showed fastest growth as compared to other edible mushrooms. It covered entire area of Petri plate (90 mm) within 4-5 days with the growth rate of 18-22.5mm/day. A unique growth pattern i.e. vertically oriented mycelia were observed. Brown coloured pigmentation in the culture was also observed during the study. The basal media for spawn (wheat grains; Glucose, 1%, CaCO3, 2%; CaSO4, 1.5% and MgSO4, 1%) was aseptically inoculated with the mushroom culture. The spawn substrate was colonized by mushroom mycelium in 7-8 days. The sclerotia were formed in unused (old) spawn. Wheat straw was used as a substrate for mushroom cultivation. It was supplemented with wheat bran, 20%, Glucose (1%) and MgSO4 (1%). It showed prolific growth when it was seeded by mushroom spawn using jar method. Once substrate was fully covered with mushroom mycelium, casing was applied. Sclerotia were successfully obtained after 14 days of incubation in our experiment both in the substrate and casing soil but failed to give rise to fruiting primordial. Further research is going on to domesticate this species in this part of country.

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3. 60
SCLEROTIA PRODUCTION: A WAY AHEAD TO MORCHELLA CULTIVATION
Siddhant 1*, P O UKAOGO2, Ruchira SINGH3 and RS KANAUJIA 4
1*
Durgesh Nandini Degree College, Former Assistant Professor, Department of Botany,
Ayodhya, India
2
Assistant Professor, Abia State University, Analytical/Environmental Unit, Department of
Pure and Industrial Chemistry, Nigeria
3
Independent Researcher, Ayodhya, India
4
Former Associate Professor, K.S.S. P.G. College, Department of Botany, Ayodhya, India
Abstract
Morchella, the true morels, belonging to Helvellaceae family of class Ascomycetes, are
amongst the most highly prized fungi in the world. Their artificial production is still a challenge,
even though patents for their cultivation do exist. The tissue of Morchella sp. was transferred
aseptically to Potato Dextrose Agar medium (peeled, sliced and boiled potato, 200 g; dextrose,
20 g; agar, 20 g L-1) to grow hyphae. The mycelium showed fastest growth as compared to
other edible mushrooms. It covered entire area of Petri plate (90 mm) within 4-5 days with the
growth rate of 18-22.5mm/day. A unique growth pattern i.e. vertically oriented mycelia were
observed. Brown coloured pigmentation in the culture was also observed during the study. The
basal media for spawn (wheat grains; Glucose, 1%, CaCO3, 2%; CaSO4, 1.5% and MgSO4, 1%)
was aseptically inoculated with the mushroom culture. The spawn substrate was colonized by
mushroom mycelium in 7-8 days. The sclerotia were formed in unused (old) spawn. Wheat
straw was used as a substrate for mushroom cultivation. It was supplemented with wheat bran,
20%, Glucose (1%) and MgSO4 (1%). It showed prolific growth when it was seeded by
mushroom spawn using jar method. Once substrate was fully covered with mushroom
mycelium, casing was applied. Sclerotia were successfully obtained after 14 days of incubation
in our experiment both in the substrate and casing soil but failed to give rise to fruiting
primordial. Further research is going on to domesticate this species in this part of country.
Keywords: Ascomycetes, Jar method, Morchella, Sclerotium.