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VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145
ARPN Journal of Agricultural and Biological Science
©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved.
www.arpnjournals.com
WATER REQUIREMENT, DEFICIT IRRIGATION AND CROP
COEFFICIENT OF HOT PEPPER (Capsicum frutescens)
USING IRRIGATION INTERVAL OF FOUR (4) DAYS
J. D. Owusu-Sekyere, P. Asante and P. Osei-Bonsu
E-Mail: jaydosus@yahoo.com
ABSTRACT
This study was conducted to determine the seasonal water requirement of pepper, the crop coefficient under full
water requirement as well as the effect of deficit irrigation on pepper growth and development under a rain shelter. The
treatments imposed were T1, application of 100% crop water requirement, T2, was 80%, T3 60% and T4 40 % of crop
water requirement. It was determined that pepper requires about 587.48mm of water over the growth season. The crop
coefficient under full water supply was found to be: 0.47, 0.86, 1.42 and 0.91 for initial, development, mid-season and the
late season stages, respectively. The study also revealed that reduction in 20% water need of hot pepper has no significant
effect on growth, development and fruiting of the crop.
Keywords: pepper, water requirement, deficit irrigation, growth, crop coefficient.
INTRODUCTION
Deficit irrigation is a strategy that allows a crop
to sustain some degree of water deficit in order to reduce
costs and potentially increase income. It can lead to
increase net income where water costs are high or where
water supplies are limited (Kirda et al., 2002).
Kang et al. (2001) conducted a hot pepper study
applying water through alternate drip irrigation on partial
roots (ADIP), fixed drip irrigation on partial roots (FDIP),
and drip irrigation on whole roots (EDIP), and concluded
that ADIP maintained high yield, with as much as 40%
reduction in irrigation compared to EDIP and FDIP;
moreover, the highest water-use efficiently occurred with
ADIP. Costa and Gianquinto (2002) reported that
continuous water stress significantly reduced total fresh
weight of fruit and that the highest marketable yield was
obtained with irrigation of 120% ET (Evapotranspiration),
lowest at 40% ET and marketable yield was the same at
60%, 80% and 100% ET. Jamez et al. (2000) revealed that
a water deficit during the period of flowering and fruit
development reduced final fruit production.
Pepper is commonly grown in drought-prone
areas of Ghana where climatic conditions are hot and
water so scarce. In these circumstances, deficit irrigation
will probably contribute to the economical use of water
sources if significant reduction does not occur in crop
yields. Many field studies of DI have been conducted.
Such field studies are needed for economic analysis of DI
and to increase net income and water-use efficiency
(English et al., 1990).
Pepper (Capsicum and Capsicum frutescens) is
thought to originate from tropical America. Most of the
peppers grown belong to Capsicum frutescens. Present
world production is about 19 million tons fresh fruit from
1.5 million ha (FAOSTAT, 2001).
One of the major setback in vegetable production
in Ghana, that is, pepper and leafy vegetables, is the
inability of farmers to determine the correct amount of
water required by the crop and adoption to the necessary
irrigation practices during the growing season so as to
maximize profit. This usually results in water stress which
directly affects crop growth and yield. Deficit irrigation is
a strategy that allows a crop to sustain some degree of
water deficit in order to reduce costs and potentially
increase income. It can lead to increase net income where
water supplies are limited (English and Raja, 1996). The
unavailability of rainfall to compensate for
evapotranspiration losses by a crop necessitates the
application of artificial irrigation if better yield is expected
by the farmer. It is in the light of this that the study was
conducted to delve into the determination of the water
requirement for pepper plant and this will help reduced the
problems encountered by commercial pepper farmers who
face much challenges in the determination and application
of correct amount of water require by the pepper plant.
MATERIALS AND METHOD
Study area
The study area was the School of Agriculture
Teaching and Research Farm at the University of Cape
Coast. Cape Coast Vegetation cover is made up of shrub.
The soil type as classified by Asamoah (1973) is a sandy
clayey loam of the Benya series, which is a member of the
Edina Benya-Udu association. The study area experiences
two rainy seasons namely the major season which starts
from May and end in July and a minor season that starts
around September and ends around mid November to give
to the dry Harmattan season that runs through the end of
March in the subsequent year. Mean annual temperature
range for the day is 300
C-340
C and that of the night is
220
C-240
C with relative humidity between 75%-79%.
Experimental design and field layout
The Randomized Block Design was used, with
four treatments (T1-T4) and three replications, (R1-R3).
Forty-eight (48) poly-bags were filled with sandy loam
soil from the experimental site weighed on a scale to a
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VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145
ARPN Journal of Agricultural and Biological Science
©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved.
www.arpnjournals.com
weight of six (6) kilograms each after which they were
placed under rain shelter where the research took place
and replicated (100%-T1, 80%-T2, 60%-T3, 40%-T4). Six
(6) boxes each measuring 1.0m x 0.95m giving a total area
of 5.7m2
divided into three (3) replications with each
containing twelve (12) nursery bags were used.
Planting
Seeds of the Legon 18 variety of hot pepper were
nursed on 18th
September, 2009 and transplanted on 8th
October, 2009. A week before transplanting; water supply
at the nursery was reduced in order to harden the seedlings
to reduce transplanting shock. Before transplanting, both
nursery and all forty-eight filled poly-bags were watered
to enhance easy uprooting of seedlings and prevent root
damage of the seedlings and also help quicken the
recovery rate after transplanting. The transplants were
subjected to equal amount of water application for seven
(7) days to ensure uniform recovery of all transplants.
Growth stages
Four growth stages were considered. They were
the initial stage, developmental stage, mid-season stage,
and late season stage. The initial stage excluding seedlings
at the nursery lasted for 15 days (October 15 - October 30,
2009). The developmental growth stage lasted for 30 days
(October 30, 2009 - November 29, 2009). The mid-season
growth stage (flowering and fruiting) stage lasted for 50
days (November 29, 2009 - January19, 2010) and the late
season stage lasted for 26 days (January19, 2010 -
February 14, 2010). This stage was later characterized by
senescence and drying of leaves after the harvesting was
over.
Irrigation regime
A four-day interval irrigation regime was adopted
and the amount of water to be applied each four-day
interval was derived from the computed loss in weight of
each set up over the four days. The equivalent in volume
basis was found and applied to the plants as the various
treatments demanded. Irrigation days amounted to 60.5
days out of the 129days of the growing period.
Determination of crop coefficient (Kc) and water
requirement (ETc) and reference evapotranspiration
(ETo)
The crop water requirement (ETc) is defined as
the depth (or amount) of water needed to meet the water
loss through evapotranspiration. This was calculated for
the initial, mid-season and the late season growth stages
for the various treatments.
ETc = ETo x Kc ........................ (1)
Kc = ETc/ Eto ........................... (2)
ETo = Epan x Kpan ................... (3)
ETc (4days) = Loss in weight of poly bags
ETc for a growth stage = 4 days ETc x Growth period.
Where
ETc - Crop evapotranspiration or Crop water requirement
(mm/day)
Kc - Crop factor
ETo - Reference crop evapotranspiration (mm/day)
K pan - Pan Coefficient (0.80)
Soil analysis
Soil samples were taken from nursery bags and
were thoroughly mixed together. The samples were
divided into four and two opposite quadrants were taken
out. This was repeated and each time, another opposite
quadrants was taken off until a substantial amount was
obtained. The sample was then dried for four days after
which it was grounded and then analyzed for the amount
of nitrogen, phosphorous and potassium as well as
moisture content and bulk density. This was done for the
three growth stages considered.
Other data collected
a. Plant height: This was measured using a rule; three
plants were selected from each treatment replication.
b. Leaf area: Five leaves from different parts of the
plants were selected on each replication. The longest
part along the petiole line of the leaf and the widest
breath across the leaf were noted and measured as the
length and breath of the leaf by using a 30 cm metre
rule. The product was multiplied by a factor of 0.75 to
get the leaf area.
c. Mean number of fruits per treatment: The number
of fruits per treatment was determined by counting the
number of harvested fruits. Mean fruit size: Mean fruit
size per plant was determined by using a veneer
calliper to transversely measure the breath.
d. Mean fruit weight: The number of fruits produced by
each of the selected plants under each treatment was
weighed by the use of an electronic analytical balance.
Reference crop evapotranspiration rate and rainfall
reading: Evaporation rate and amount of rainfall
readings were obtained from the US Class A
evaporation pan and a rain gauge respectively situated
at the farm where the experiment was conducted. To
obtain the reference crop evaporation. 0.8 was chosen
as the pan factor because the experimental location
had a moderate wind speed of 2-3 ms-1
and high
humidity of 75-79%.
Statistical analysis
Data collected were subjected to analysis of
variance and the means were separated by Duncan’s
Multiple Range Test at a probability level of 0.05.
RESULTS AND DISCUSSIONS
According to Iwena (2002), hot pepper requires
1000 to 1500mm of water during the growing season.
According to FAO (1999) however, when the crop is
73
VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145
ARPN Journal of Agricultural and Biological Science
©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved.
www.arpnjournals.com
grown extensively under rain fed conditions, high yields
are obtained with rainfall of 600mm to 900mm, well
distributed over the growing season. Huguez and Philippe
(1998) also indicate that the total water requirements are
750mm to 900mm and up to 1250mm for long growing
periods and several pickings. Agodzo et al. (2003) indicate
that the crop water requirements range between 300mm -
700mm depending on the climatic condition and the
season of the crop and the location. Grimes and Williams
(1990) also asserted that water requirement for hot pepper
per growing season ranges between 400 mm and 500 mm
depending on the season of planting and the climatic
conditions prevailing in the area.
The results obtained from this study shows that
when the crop is given its full water requirement, 587.48
mm of water is required, but a Figure of 439.79mm is
required when deficit irrigation of up to 40% of the crop
water requirement is applied. Comparing these values to
those obtained by other experimenters, it can be concluded
that water requirement for pepper in the Cape Coast area
compares well with results obtained Agodzo et al. (2003).
This Figure is however lower than those obtained with the
other researchers.
In terms of the crop coefficient, Freeira and
Goncalves (2005) obtained 0.3, 1.22 and 0.65. According
to FAO (1999) Kc is 0.4 following transplanting, 1.1
during full cover and 0.9 at time of harvest. The crop
coefficient (Kc) is affected by a number of factors, which
include: the type of crop, stage of growth of the crop and
the cropping pattern (Allen and Smith, 1998). Doorenbos
and Pruitt (2000) indicated that plant height and total
growing season influence crop coefficient values. The
higher the plant height and the longer the growing season
the higher the crop coefficient values and vice versa.
In this study, Kc was 0.47, 0.86, 1.42 and 0.91 for
initial, development, mid-season and the late season
stages, respectively as indicated in Table-1. These values
compare quite well with those obtained by FAO (1999)
where the water requirement was 600 mm for the growing
period of 120 days.
Table-1. Growth period, ETo, Kc and Etc for all the growth stages.
Growth
stage
Period
(days)
ETc
(100%)
ETc
(80%)
ETc
(60%)
ETc
(40%)
ETo
Kc
(100%)
Kc
(80%)
Kc
(60%)
Kc
(40%)
Initial 17 32.95 28.74 26.64 25.24 70.10 0.47 0.41 0.38 0.36
Dev. 32 115.84 84.86 105.07 96.98 134.70 0.86 0.63 0.78 0.72
Mid. 56 343.78 295.36 249.36 237.26 242.10 1.42 1.22 1.03 0.98
Late 24 94.91 87.61 84.84 80.31 104.30 0.91 0.84 0.81 0.77
Sum 587.48 496.57 465.91 439.79
NPK levels
Soil NPK levels for the initial, mid-stage as well
as the last stage are shown in Figures 1, 2 and 3. The
uptake of nutrients such as nitrogen, phosphorus and
potassium by plants are influenced by the amount of water
available in the soil. Broeshart et al. (1965) found that
flooding in a rice farm increased the uptake of potassium.
Adequate amount of water in the soil tend to enhance
aeration and this according to Cline and Erickson (1956),
would improve potassium and nitrogen uptake. Shapiro et
al. (1956) indicated that translocation of phosphorus
increases when there is improvement in aeration. The
results obtained from the study indicates that T4 utilised
the most N, where as T2 and T1 the most P. With regards
to K, utilisation was greatest under T4.
Figure-1. levels of Nitrogen in the soil for the entire growth period.
74
VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145
ARPN Journal of Agricultural and Biological Science
©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved.
www.arpnjournals.com
Figure-2. levels of phosphorus in the soil for the entire growth period.
Figure-3. levels of potassium in the soil for the entire growth period.
Plant height and leaf area
At the end of the growth period, mean plant
height was in the order T2>T1>T3>T4 and mean plant
leaf area in the order T1>T2>T4>T3 (Table-2).
Interestingly however, in both cases the results were not
significantly different at the 5% probability level in spite
of the large differences in amount of water applied.
Table-2. Mean plant height (MPH) and leaf area (LA) for the treatments at various stages of plant growth.
Treatments Initial stage
MPH LA
(cm) (cm2
)
Developmental
MPH LA
(cm) (cm2
)
Mid-season
MPH LA
(cm) (cm2
)
Late season
MPH LA
(cm) (cm2
)
T1 7.85a 5.84a 21.56a 7.10a 31.96a 9.95a 33.73a 10.23a
T2 8.13a 6.46a 22.80a 7.12a 32.26a 9.61a 33.50a 10.11a
T3 7.04a 7.84a 20.76a 8.01a 31.66a 9.65a 32.62a 10.08a
T4 6.98a 6.93a 19.36a 7.01a 30.93a 9.04a 31.63a 9.56a
Probability = 0.05 Probability = 0.05 Probability = 0.05 Probability = 0.05
Pepper leaves photosynthesize more efficiently
when water is abundant, resulting in a higher percentage
of large, heavy, marketable fruits (Alvino et al., 1994).
Under water stress, the products of photosynthesis are
fewer; fruit growth and development are inhibited, and
yield is decreased (Bray, 1997). Chlorophyll destruction is
quickened by moisture stress (Alberte et al., 1997).
More severe and prolonged water stress may
result in poor flower-cluster development and reduced
pistil and pollen viability and subsequent fruit set (Falcetti
et al., 1995). Following fruit set, severe water stress may
75
VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145
ARPN Journal of Agricultural and Biological Science
©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved.
www.arpnjournals.com
cause flower abortion and cluster abscission, possibly
associated with hormone changes (During, 1986).
Uncorrected water stress during this stage of development
may result in reduced canopy development and,
consequently, insufficient leaf area to adequately support
fruit development and maturation. Interestingly however,
there were no significant differences in leaf area in spite of
the water stress imposed.
Yield components
The relationships between crop yield and water
use are complicated. Yield may depend on the timing of
water application or on the amount applied. Information
on optimal scheduling of limited amounts of water to
maximize yields of high quality crops are essential if
irrigation water is to be used most efficiently (Anaç et al.,
1997). Timing, duration and the degree of water stress all
affect crop yield.
Mean number of fruits
It can be seen from Table-3 that T1 produced the
highest mean number of fruits (16.67) followed by T2
13.91 fruits, then T3, 9.52 fruits, and lastly T4 with 4.61
fruits. There was no significant difference between T1 and
T2, but T1 differed significantly from T3 and T4. T2 was
however not significantly different from T3.
According to Fisher et al. (1985) in an
experiment conducted, highest yields were obtained from
highest regulated irrigation regime and lowest yield was
obtained from the lowest irrigation water applied.
Reducing irrigation water application by 40% resulted in
30% decrease in marketable fruit yield. As long as soil
moisture is maintained throughout the growing season the
roots will be able to maintain an adequate flow of water to
the leaves to maintain growth. At the mid-season and the
late season stages, T1 utilized available nitrogen
effectively (Figure-1) which may have influenced the
highest number of fruits formed since nitrogen is a
component of amino acids and proteins and so forms
essential part of protoplasm, enzymes which are stored
food for fruit development.
Factors that could be responsible for the low fruit
numbers include blossom drop, a situation whereby all
cells and tissues at the distal and blossoms end of the
plants stems fail to receive enough moisture to maintain
their body, grow and develop, and so leads to cell
breakdown, flower abortion and its subsequent drop
(Berrie et al., 1990). These were observed on some
treatment levels at varying degrees, and were highest
especially under T4 four where the drought stress coupled
with the higher night temperatures favoured flower failure
and this could be responsible for lowest number of fruits
obtained.
The results of the study are in agreement with the
results of the study by Pill and Lambeth (1980) who
observed a reduction in the fruit number with decreasing
soil water, explaining that lower soil moisture could result
in pollen and stigma dehydration as well as unnecessary
elongation of the flower’s style which could result in up to
50% reduction in fruit setting and final fruit yield.
Table-3. Mean values of yield components for the treatments.
Treatment
Mean number of
fruits
Mean fruit size
(mm)
Mean fruit
weight (kg)
Mean yield per
total area (tons/ha)
T1 16.67a 17.8a 0.145a 1.45a
T2 13.91ab 12.3ab 0.128a 1.28a
T3 9.52b 6.8bc 0.080bc 0.80 bc
T4 4.61c 3.5c 0.039c 0.39c
Prob.= 0.05 Prob.= 0.05 Prob.=0.05 Prob.=0.05
Treatment means followed by the same letter are not significantly different at 5% probability level.
Fruit weight
T1 produced the heaviest fruits weighing 0.145
Kg. This was followed by T2 weighing 0.128kg while T3
recorded a mean weight of 0.080kg and the lowest mean
fruit weight was recorded by T4 which was 0.039kg. T1
was not significantly different from T2 but was
significantly different form T3 and T4. However, T3 and
T4 were not significantly different from each other.
Plants can make virtually everything they need
from water and air with a few nutrients that the roots
absorb from the soil. The plant uses sunlight to split water
into hydrogen and oxygen. It discards the oxygen as a
waste product. The plant uses the hydrogen to make sugar
from carbon dioxide in the air. Plants use oxygen in the air
to burn sugar and make energy to live. The sole purpose of
the leaves is to harvest light and make sugar (Longstroth,
1996) When the rate of photosynthesis is reduced as a
result of reduced amount of water, the sensitive
phytochrome pigments (chlorophyll pigmentation) that
intercepts light for the process is affected then plants
subjected to drought stress should be expected to have
small and light fruits weights (Pill and Lambeth, 1980).
Mean fruit size
T1 produced the highest fruits size 17.8mm. This
was followed by T2 (12.3mm) while T3 recorded a mean
fruit size of 6.8mm and the lowest mean fruit size was
recorded by T4 which was 3.5mm. The results also
indicated that no significant difference exist in the mean
fruit sizes of T1and T2 but was significantly different from
76
VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145
ARPN Journal of Agricultural and Biological Science
©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved.
www.arpnjournals.com
that of T3 and T4. Pill and Lambeth (1980) investigated
the effect of water on plants and concluded that water
stress is capable of restricting plants to achieve their full
genetic potential. As noted by Longstroth (1996) the early
period of fruit growth is very important in determining
final fruit size. For about a month after bloom the fruit
grows by cell division. Later, the fruit grows by cell
enlargement. So, two factors influence fruit size, cell
number and cell size. Bigger fruits have more cells, so the
final fruit size is determined in the month after bloom.
Lack of water reduces the growth of new shoots and
leaves. This means that there is less sugar to be used for
fruit growth hence smaller sizes. Their work support the
findings made in this study. Plants that were given full
water application yielded the largest fruits while the
opposite was true for treatment four which received the
least amount of water applied.
CONCLUSIONS
The water requirement and crop coefficient of hot
pepper were determined for the various growth stages
using an irrigation interval of four days. At 100% water
application (full irrigation), crop coefficient for hot pepper
was determined to be 0.47, 0.86, 1.42 and 0.91 for initial,
development, mid-season and the late season stages
respectively and the total amount of water applied for the
129 days was 587.48mm. It is also important to note that
reducing water application by 20% has no significant
reduction on the yield of hot pepper but above this has
adverse effect on the plant and yield as indicated by
treatment four recording the lowest yield of 0.39. As a
result, 20% reduction in water application could be
recommended for deficit irrigation in hot pepper
production.
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Jabs 0910 213

  • 1. VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145 ARPN Journal of Agricultural and Biological Science ©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved. www.arpnjournals.com WATER REQUIREMENT, DEFICIT IRRIGATION AND CROP COEFFICIENT OF HOT PEPPER (Capsicum frutescens) USING IRRIGATION INTERVAL OF FOUR (4) DAYS J. D. Owusu-Sekyere, P. Asante and P. Osei-Bonsu E-Mail: jaydosus@yahoo.com ABSTRACT This study was conducted to determine the seasonal water requirement of pepper, the crop coefficient under full water requirement as well as the effect of deficit irrigation on pepper growth and development under a rain shelter. The treatments imposed were T1, application of 100% crop water requirement, T2, was 80%, T3 60% and T4 40 % of crop water requirement. It was determined that pepper requires about 587.48mm of water over the growth season. The crop coefficient under full water supply was found to be: 0.47, 0.86, 1.42 and 0.91 for initial, development, mid-season and the late season stages, respectively. The study also revealed that reduction in 20% water need of hot pepper has no significant effect on growth, development and fruiting of the crop. Keywords: pepper, water requirement, deficit irrigation, growth, crop coefficient. INTRODUCTION Deficit irrigation is a strategy that allows a crop to sustain some degree of water deficit in order to reduce costs and potentially increase income. It can lead to increase net income where water costs are high or where water supplies are limited (Kirda et al., 2002). Kang et al. (2001) conducted a hot pepper study applying water through alternate drip irrigation on partial roots (ADIP), fixed drip irrigation on partial roots (FDIP), and drip irrigation on whole roots (EDIP), and concluded that ADIP maintained high yield, with as much as 40% reduction in irrigation compared to EDIP and FDIP; moreover, the highest water-use efficiently occurred with ADIP. Costa and Gianquinto (2002) reported that continuous water stress significantly reduced total fresh weight of fruit and that the highest marketable yield was obtained with irrigation of 120% ET (Evapotranspiration), lowest at 40% ET and marketable yield was the same at 60%, 80% and 100% ET. Jamez et al. (2000) revealed that a water deficit during the period of flowering and fruit development reduced final fruit production. Pepper is commonly grown in drought-prone areas of Ghana where climatic conditions are hot and water so scarce. In these circumstances, deficit irrigation will probably contribute to the economical use of water sources if significant reduction does not occur in crop yields. Many field studies of DI have been conducted. Such field studies are needed for economic analysis of DI and to increase net income and water-use efficiency (English et al., 1990). Pepper (Capsicum and Capsicum frutescens) is thought to originate from tropical America. Most of the peppers grown belong to Capsicum frutescens. Present world production is about 19 million tons fresh fruit from 1.5 million ha (FAOSTAT, 2001). One of the major setback in vegetable production in Ghana, that is, pepper and leafy vegetables, is the inability of farmers to determine the correct amount of water required by the crop and adoption to the necessary irrigation practices during the growing season so as to maximize profit. This usually results in water stress which directly affects crop growth and yield. Deficit irrigation is a strategy that allows a crop to sustain some degree of water deficit in order to reduce costs and potentially increase income. It can lead to increase net income where water supplies are limited (English and Raja, 1996). The unavailability of rainfall to compensate for evapotranspiration losses by a crop necessitates the application of artificial irrigation if better yield is expected by the farmer. It is in the light of this that the study was conducted to delve into the determination of the water requirement for pepper plant and this will help reduced the problems encountered by commercial pepper farmers who face much challenges in the determination and application of correct amount of water require by the pepper plant. MATERIALS AND METHOD Study area The study area was the School of Agriculture Teaching and Research Farm at the University of Cape Coast. Cape Coast Vegetation cover is made up of shrub. The soil type as classified by Asamoah (1973) is a sandy clayey loam of the Benya series, which is a member of the Edina Benya-Udu association. The study area experiences two rainy seasons namely the major season which starts from May and end in July and a minor season that starts around September and ends around mid November to give to the dry Harmattan season that runs through the end of March in the subsequent year. Mean annual temperature range for the day is 300 C-340 C and that of the night is 220 C-240 C with relative humidity between 75%-79%. Experimental design and field layout The Randomized Block Design was used, with four treatments (T1-T4) and three replications, (R1-R3). Forty-eight (48) poly-bags were filled with sandy loam soil from the experimental site weighed on a scale to a 72
  • 2. VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145 ARPN Journal of Agricultural and Biological Science ©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved. www.arpnjournals.com weight of six (6) kilograms each after which they were placed under rain shelter where the research took place and replicated (100%-T1, 80%-T2, 60%-T3, 40%-T4). Six (6) boxes each measuring 1.0m x 0.95m giving a total area of 5.7m2 divided into three (3) replications with each containing twelve (12) nursery bags were used. Planting Seeds of the Legon 18 variety of hot pepper were nursed on 18th September, 2009 and transplanted on 8th October, 2009. A week before transplanting; water supply at the nursery was reduced in order to harden the seedlings to reduce transplanting shock. Before transplanting, both nursery and all forty-eight filled poly-bags were watered to enhance easy uprooting of seedlings and prevent root damage of the seedlings and also help quicken the recovery rate after transplanting. The transplants were subjected to equal amount of water application for seven (7) days to ensure uniform recovery of all transplants. Growth stages Four growth stages were considered. They were the initial stage, developmental stage, mid-season stage, and late season stage. The initial stage excluding seedlings at the nursery lasted for 15 days (October 15 - October 30, 2009). The developmental growth stage lasted for 30 days (October 30, 2009 - November 29, 2009). The mid-season growth stage (flowering and fruiting) stage lasted for 50 days (November 29, 2009 - January19, 2010) and the late season stage lasted for 26 days (January19, 2010 - February 14, 2010). This stage was later characterized by senescence and drying of leaves after the harvesting was over. Irrigation regime A four-day interval irrigation regime was adopted and the amount of water to be applied each four-day interval was derived from the computed loss in weight of each set up over the four days. The equivalent in volume basis was found and applied to the plants as the various treatments demanded. Irrigation days amounted to 60.5 days out of the 129days of the growing period. Determination of crop coefficient (Kc) and water requirement (ETc) and reference evapotranspiration (ETo) The crop water requirement (ETc) is defined as the depth (or amount) of water needed to meet the water loss through evapotranspiration. This was calculated for the initial, mid-season and the late season growth stages for the various treatments. ETc = ETo x Kc ........................ (1) Kc = ETc/ Eto ........................... (2) ETo = Epan x Kpan ................... (3) ETc (4days) = Loss in weight of poly bags ETc for a growth stage = 4 days ETc x Growth period. Where ETc - Crop evapotranspiration or Crop water requirement (mm/day) Kc - Crop factor ETo - Reference crop evapotranspiration (mm/day) K pan - Pan Coefficient (0.80) Soil analysis Soil samples were taken from nursery bags and were thoroughly mixed together. The samples were divided into four and two opposite quadrants were taken out. This was repeated and each time, another opposite quadrants was taken off until a substantial amount was obtained. The sample was then dried for four days after which it was grounded and then analyzed for the amount of nitrogen, phosphorous and potassium as well as moisture content and bulk density. This was done for the three growth stages considered. Other data collected a. Plant height: This was measured using a rule; three plants were selected from each treatment replication. b. Leaf area: Five leaves from different parts of the plants were selected on each replication. The longest part along the petiole line of the leaf and the widest breath across the leaf were noted and measured as the length and breath of the leaf by using a 30 cm metre rule. The product was multiplied by a factor of 0.75 to get the leaf area. c. Mean number of fruits per treatment: The number of fruits per treatment was determined by counting the number of harvested fruits. Mean fruit size: Mean fruit size per plant was determined by using a veneer calliper to transversely measure the breath. d. Mean fruit weight: The number of fruits produced by each of the selected plants under each treatment was weighed by the use of an electronic analytical balance. Reference crop evapotranspiration rate and rainfall reading: Evaporation rate and amount of rainfall readings were obtained from the US Class A evaporation pan and a rain gauge respectively situated at the farm where the experiment was conducted. To obtain the reference crop evaporation. 0.8 was chosen as the pan factor because the experimental location had a moderate wind speed of 2-3 ms-1 and high humidity of 75-79%. Statistical analysis Data collected were subjected to analysis of variance and the means were separated by Duncan’s Multiple Range Test at a probability level of 0.05. RESULTS AND DISCUSSIONS According to Iwena (2002), hot pepper requires 1000 to 1500mm of water during the growing season. According to FAO (1999) however, when the crop is 73
  • 3. VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145 ARPN Journal of Agricultural and Biological Science ©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved. www.arpnjournals.com grown extensively under rain fed conditions, high yields are obtained with rainfall of 600mm to 900mm, well distributed over the growing season. Huguez and Philippe (1998) also indicate that the total water requirements are 750mm to 900mm and up to 1250mm for long growing periods and several pickings. Agodzo et al. (2003) indicate that the crop water requirements range between 300mm - 700mm depending on the climatic condition and the season of the crop and the location. Grimes and Williams (1990) also asserted that water requirement for hot pepper per growing season ranges between 400 mm and 500 mm depending on the season of planting and the climatic conditions prevailing in the area. The results obtained from this study shows that when the crop is given its full water requirement, 587.48 mm of water is required, but a Figure of 439.79mm is required when deficit irrigation of up to 40% of the crop water requirement is applied. Comparing these values to those obtained by other experimenters, it can be concluded that water requirement for pepper in the Cape Coast area compares well with results obtained Agodzo et al. (2003). This Figure is however lower than those obtained with the other researchers. In terms of the crop coefficient, Freeira and Goncalves (2005) obtained 0.3, 1.22 and 0.65. According to FAO (1999) Kc is 0.4 following transplanting, 1.1 during full cover and 0.9 at time of harvest. The crop coefficient (Kc) is affected by a number of factors, which include: the type of crop, stage of growth of the crop and the cropping pattern (Allen and Smith, 1998). Doorenbos and Pruitt (2000) indicated that plant height and total growing season influence crop coefficient values. The higher the plant height and the longer the growing season the higher the crop coefficient values and vice versa. In this study, Kc was 0.47, 0.86, 1.42 and 0.91 for initial, development, mid-season and the late season stages, respectively as indicated in Table-1. These values compare quite well with those obtained by FAO (1999) where the water requirement was 600 mm for the growing period of 120 days. Table-1. Growth period, ETo, Kc and Etc for all the growth stages. Growth stage Period (days) ETc (100%) ETc (80%) ETc (60%) ETc (40%) ETo Kc (100%) Kc (80%) Kc (60%) Kc (40%) Initial 17 32.95 28.74 26.64 25.24 70.10 0.47 0.41 0.38 0.36 Dev. 32 115.84 84.86 105.07 96.98 134.70 0.86 0.63 0.78 0.72 Mid. 56 343.78 295.36 249.36 237.26 242.10 1.42 1.22 1.03 0.98 Late 24 94.91 87.61 84.84 80.31 104.30 0.91 0.84 0.81 0.77 Sum 587.48 496.57 465.91 439.79 NPK levels Soil NPK levels for the initial, mid-stage as well as the last stage are shown in Figures 1, 2 and 3. The uptake of nutrients such as nitrogen, phosphorus and potassium by plants are influenced by the amount of water available in the soil. Broeshart et al. (1965) found that flooding in a rice farm increased the uptake of potassium. Adequate amount of water in the soil tend to enhance aeration and this according to Cline and Erickson (1956), would improve potassium and nitrogen uptake. Shapiro et al. (1956) indicated that translocation of phosphorus increases when there is improvement in aeration. The results obtained from the study indicates that T4 utilised the most N, where as T2 and T1 the most P. With regards to K, utilisation was greatest under T4. Figure-1. levels of Nitrogen in the soil for the entire growth period. 74
  • 4. VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145 ARPN Journal of Agricultural and Biological Science ©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved. www.arpnjournals.com Figure-2. levels of phosphorus in the soil for the entire growth period. Figure-3. levels of potassium in the soil for the entire growth period. Plant height and leaf area At the end of the growth period, mean plant height was in the order T2>T1>T3>T4 and mean plant leaf area in the order T1>T2>T4>T3 (Table-2). Interestingly however, in both cases the results were not significantly different at the 5% probability level in spite of the large differences in amount of water applied. Table-2. Mean plant height (MPH) and leaf area (LA) for the treatments at various stages of plant growth. Treatments Initial stage MPH LA (cm) (cm2 ) Developmental MPH LA (cm) (cm2 ) Mid-season MPH LA (cm) (cm2 ) Late season MPH LA (cm) (cm2 ) T1 7.85a 5.84a 21.56a 7.10a 31.96a 9.95a 33.73a 10.23a T2 8.13a 6.46a 22.80a 7.12a 32.26a 9.61a 33.50a 10.11a T3 7.04a 7.84a 20.76a 8.01a 31.66a 9.65a 32.62a 10.08a T4 6.98a 6.93a 19.36a 7.01a 30.93a 9.04a 31.63a 9.56a Probability = 0.05 Probability = 0.05 Probability = 0.05 Probability = 0.05 Pepper leaves photosynthesize more efficiently when water is abundant, resulting in a higher percentage of large, heavy, marketable fruits (Alvino et al., 1994). Under water stress, the products of photosynthesis are fewer; fruit growth and development are inhibited, and yield is decreased (Bray, 1997). Chlorophyll destruction is quickened by moisture stress (Alberte et al., 1997). More severe and prolonged water stress may result in poor flower-cluster development and reduced pistil and pollen viability and subsequent fruit set (Falcetti et al., 1995). Following fruit set, severe water stress may 75
  • 5. VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145 ARPN Journal of Agricultural and Biological Science ©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved. www.arpnjournals.com cause flower abortion and cluster abscission, possibly associated with hormone changes (During, 1986). Uncorrected water stress during this stage of development may result in reduced canopy development and, consequently, insufficient leaf area to adequately support fruit development and maturation. Interestingly however, there were no significant differences in leaf area in spite of the water stress imposed. Yield components The relationships between crop yield and water use are complicated. Yield may depend on the timing of water application or on the amount applied. Information on optimal scheduling of limited amounts of water to maximize yields of high quality crops are essential if irrigation water is to be used most efficiently (Anaç et al., 1997). Timing, duration and the degree of water stress all affect crop yield. Mean number of fruits It can be seen from Table-3 that T1 produced the highest mean number of fruits (16.67) followed by T2 13.91 fruits, then T3, 9.52 fruits, and lastly T4 with 4.61 fruits. There was no significant difference between T1 and T2, but T1 differed significantly from T3 and T4. T2 was however not significantly different from T3. According to Fisher et al. (1985) in an experiment conducted, highest yields were obtained from highest regulated irrigation regime and lowest yield was obtained from the lowest irrigation water applied. Reducing irrigation water application by 40% resulted in 30% decrease in marketable fruit yield. As long as soil moisture is maintained throughout the growing season the roots will be able to maintain an adequate flow of water to the leaves to maintain growth. At the mid-season and the late season stages, T1 utilized available nitrogen effectively (Figure-1) which may have influenced the highest number of fruits formed since nitrogen is a component of amino acids and proteins and so forms essential part of protoplasm, enzymes which are stored food for fruit development. Factors that could be responsible for the low fruit numbers include blossom drop, a situation whereby all cells and tissues at the distal and blossoms end of the plants stems fail to receive enough moisture to maintain their body, grow and develop, and so leads to cell breakdown, flower abortion and its subsequent drop (Berrie et al., 1990). These were observed on some treatment levels at varying degrees, and were highest especially under T4 four where the drought stress coupled with the higher night temperatures favoured flower failure and this could be responsible for lowest number of fruits obtained. The results of the study are in agreement with the results of the study by Pill and Lambeth (1980) who observed a reduction in the fruit number with decreasing soil water, explaining that lower soil moisture could result in pollen and stigma dehydration as well as unnecessary elongation of the flower’s style which could result in up to 50% reduction in fruit setting and final fruit yield. Table-3. Mean values of yield components for the treatments. Treatment Mean number of fruits Mean fruit size (mm) Mean fruit weight (kg) Mean yield per total area (tons/ha) T1 16.67a 17.8a 0.145a 1.45a T2 13.91ab 12.3ab 0.128a 1.28a T3 9.52b 6.8bc 0.080bc 0.80 bc T4 4.61c 3.5c 0.039c 0.39c Prob.= 0.05 Prob.= 0.05 Prob.=0.05 Prob.=0.05 Treatment means followed by the same letter are not significantly different at 5% probability level. Fruit weight T1 produced the heaviest fruits weighing 0.145 Kg. This was followed by T2 weighing 0.128kg while T3 recorded a mean weight of 0.080kg and the lowest mean fruit weight was recorded by T4 which was 0.039kg. T1 was not significantly different from T2 but was significantly different form T3 and T4. However, T3 and T4 were not significantly different from each other. Plants can make virtually everything they need from water and air with a few nutrients that the roots absorb from the soil. The plant uses sunlight to split water into hydrogen and oxygen. It discards the oxygen as a waste product. The plant uses the hydrogen to make sugar from carbon dioxide in the air. Plants use oxygen in the air to burn sugar and make energy to live. The sole purpose of the leaves is to harvest light and make sugar (Longstroth, 1996) When the rate of photosynthesis is reduced as a result of reduced amount of water, the sensitive phytochrome pigments (chlorophyll pigmentation) that intercepts light for the process is affected then plants subjected to drought stress should be expected to have small and light fruits weights (Pill and Lambeth, 1980). Mean fruit size T1 produced the highest fruits size 17.8mm. This was followed by T2 (12.3mm) while T3 recorded a mean fruit size of 6.8mm and the lowest mean fruit size was recorded by T4 which was 3.5mm. The results also indicated that no significant difference exist in the mean fruit sizes of T1and T2 but was significantly different from 76
  • 6. VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145 ARPN Journal of Agricultural and Biological Science ©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved. www.arpnjournals.com that of T3 and T4. Pill and Lambeth (1980) investigated the effect of water on plants and concluded that water stress is capable of restricting plants to achieve their full genetic potential. As noted by Longstroth (1996) the early period of fruit growth is very important in determining final fruit size. For about a month after bloom the fruit grows by cell division. Later, the fruit grows by cell enlargement. So, two factors influence fruit size, cell number and cell size. Bigger fruits have more cells, so the final fruit size is determined in the month after bloom. Lack of water reduces the growth of new shoots and leaves. This means that there is less sugar to be used for fruit growth hence smaller sizes. Their work support the findings made in this study. Plants that were given full water application yielded the largest fruits while the opposite was true for treatment four which received the least amount of water applied. CONCLUSIONS The water requirement and crop coefficient of hot pepper were determined for the various growth stages using an irrigation interval of four days. At 100% water application (full irrigation), crop coefficient for hot pepper was determined to be 0.47, 0.86, 1.42 and 0.91 for initial, development, mid-season and the late season stages respectively and the total amount of water applied for the 129 days was 587.48mm. It is also important to note that reducing water application by 20% has no significant reduction on the yield of hot pepper but above this has adverse effect on the plant and yield as indicated by treatment four recording the lowest yield of 0.39. As a result, 20% reduction in water application could be recommended for deficit irrigation in hot pepper production. REFERENCES Agodzo SK, Huibers FP, Chenini F, van Lier JB, Duran A. 2003. Use of wastewater in irrigated agriculture. Country studies from Bolivia, Ghana and Tunisia. Vol. 2: Ghana. Wageningen: WUR: p. 36. Alberte R. S., Thornber J. P. and Fiscus E.C. 1997. Water stress, effects on the content and organization of chlorophyll in the mesophyll and bundle sheath chloroplast. Plant Physiology. 59: 351. Allen R, Pereira L, Raes D, Smith M. 1998. Crop evapotranspiration: guidelines for computing crop water requirements. FAO Irrigation and Drainage Paper No. 56, FAO, Rome, Italy. Alvino A., Centritto M. and Lerenzi F. 1994. Photosynthesis response of sunlight and shade pepper leaves at different positions in the canopy under two water regimes. 21: 377-391. Anaç M.S., Ali Ul M., Tuzal I.H., Anac D., Okur B. and Hakerlerler H. 1999. Optimum irrigation schedules for cotton under deficit irrigation conditions. In: C. Kirda, P. Moutonnet, C. Hera, D.R Nielsen, (Eds.). Crop Yield Response to Deficit Irrigation. Dordrecht, the Netherlands, Kluwer Academic Publishers. pp. 196-212. Asamoah G.K. 1973. Soils of the proposed farm site of the University of Cape Coast. Soil Research Institute (C.S.I.R) Technical Report No. 88. AVRDC. 2006. Report. Asian Vegetable Research and Development Centre, Shanhua, Tainan, Taiwan, ROC. pp. 298-301. Berrie G. K., Berie A. and Eze J. M. 1990. Tropical plant science. 2nd Ed. Longman group, UK. Bray E.A. 1997. Plant responses to water deficit. Trends Plant. Sci. 2: 48-54. Broeshart H., Haunold E. and Fried M. 1965. The effect of water conditions and oxidation status of rice soils on the availability of soil and fertilizer phosphate. Plant and Soil. 23(3): 305-313. Cline R. A. and Erickson A. E. 1959. The effect of oxygen diffusion rate and applied fertilizer on the growth, yield and chemical composition of peas. Soil Sci. Soc. Amer. Proc. 23: 331-335. Della Costa, L. and Gianquinto G. 2002. Water Stress and Water Table Depth Influence Yield, Water Use Efficiency, and Nitrogen Recovery in Bell Pepper. Doorenbos J. and Pruitt W.O. 1979. The mechanism of regulation of ‘Bartlett’ pear fruit and vegetative growth by irrigation withholding and regulated deficit irrigation. Journal of American Society of Horticultural Science. 111: 904. During H. 1986. ABA and water stress in grapevines. Acta Horticulture. 179: 413. English M. 1990. Deficit irrigation. I. Analytical framework. Journal of Irrigation and Drainage Engineering. 116: 399-412. Falcetti M., Stringari G., Bogoni M. and Scienza A. 1995. Relationships among pedo-climatic conditions, plant available water and nutritional status of grapevines. Acta Horticulture. 383: 289-297. FAO. 2003. Agriculture, food and water, Rome, Italy. FAOSTAT. 2001. Hot pepper production in sub-Saharan Africa. A Report. Journal of Crop Science. Food and Agriculture Organization. p. 23. FAOSTAT. 2001. FAO, Rome, Italy. 77
  • 7. VOL. 5, NO. 5, SEPTEMBER 2010 ISSN 1990-6145 ARPN Journal of Agricultural and Biological Science ©2006-2010 Asian Research Publishing Network (ARPN). All rights reserved. www.arpnjournals.com Fereres E., F. Orgaz and F.J. Villalobos. 1993. Water Use Efficiency in Sustainable Agricultural Systems. International Crop Science. (1993): 83-89. Fisher K.H., Cline R.A. and Bradt O.A. 1985. The effects of trickle irrigation and training systems on the performance of concord grapes. Drip/Trickle Irrigation in Action. 1: 220-230. Grimes D.W. and L.E. Williams. 1990. Irrigation effects on plant. Water relations and Seedless grapevines. Crop Sci. 30: 255-260. Huguez D. and Philippe De Leenor. 1998. Ways of Water; Run-off, Irrigation and Drainage. 4th Ed. Imperimie Guyot Braine-le-Chateau, Belgium. Iwena O.A. 2002. Essential Agricultural Science for Senior Secondary Schools. Tonad Publishers Ltd., Ikeja. pp. 55-63. Jaimez R.E., Vielma O., Rada F. and Garcia-Nunez.C. 2000. Effects of Water Deficit on the Dynamics of Flowering and Fruit Production in Capsicum. Chinese Jacque in a tropical semi-arid region of Venezuela. J. Agronomy Crop Science. 185: 113-119. Kang S., Zhang L., Hu X., Li Z. and Jerie P. 2001. An improved Water Use Efficiency for Hot Pepper grown under controlled alternate drip irrigation on partial roots. Scientia Horticulture. 89: 257-267. Kirda C., Moutonnet P., Hera C. and Nielsen D.R. 2002. Crop yield response to deficit irrigation. Dordrecht, The Netherlands, Kluwer Academic Publishers. Levetin E. and Mcmahon K. 1999. Plant and Society. 2nd Ed. Mc GrawHill Boston WCB. pp. 279-281. Longstroth H. 1996. Effect of soil moisture and nitrogen on plant growth, mineral composition and productivity of bell pepper (Capsicum frutescens). American Journal of Agronomy and Horticulture. 21: 13-17. Pill W.G. and Lambeth V.N. 1980. Effects of soil water regime and yield, water relations and elemental composition of tomato. J. Am. Soc. Hort. Sci. 105: 730- 734. Shapiro R. E., Taylor G. S. and Volk G. W. 1956. Soil oxygen contents and ion uptake by corn. Soil Sci. Soc. Amer. Proc. 20: 193-197. 78