Prosedur Pembuatan Reagent Untuk Pewarnaan

1. Prosedur Pembuatan
Reagent Untuk Pewarnaan .
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2. Daftar Isi
Acid Fast Stain / Pewarnaan Bakteri Tahan Asam.
Capsule Stain / Pewarnaan Kapsul.
Fungal Stain / Pewarnaan Jamur.
Gram Stain / Pewarnaan Gram.

3. Daftar Isi
Negative Stain / Pewarnaan Negatif.
Spore Stain / Pewarnaan Spora.

4. Acid-fast Stain

5. Solution A
 Basic Fuchsin (90% dye content) = 0,3 g
 Ethyl alcohol (95%) = 10,0 ml
Solution B
 Phenol = 5,0 g
 Distilled water = 95,0 ml
Note : Mix solutions A and B. Add 2 drops of trition X
per 100 ml of stain for use in heatless method

6. 

Ethyl alcohol (95%) = 97,0 ml
Hydrochloric acid = 3,0 ml

7. 

Methylene Blue = 0,3 g
Didtilled water = 100,0 ml

8. Capsule Stain

9. 

Crystal Violet (85% dye Content) = 1,0 g
Distilles Water = 100,0 ml

10. 

Copper Sulfate (CuSO₄ . 5H₂O) = 20,0 g
Distilled water = 80,0 ml

11. Fungal Stains

12. Lactic acid = 20,0 ml
 Phenol = 20,0 g
 Glycerol = 40,0 ml
 Distilled water = 20,0 ml
 Aniline Blue = 0,05 g
Note : Heat Gently in hot water (double boiler) to
dissolve; then add aniline blue dye


13. 

Gram`s iodine (as in Gram`s stain) = 10,0 ml
Distilled water = 30,0 ml

14. Gram Stain

15. Solution A
 Crystal Violet (90% dye content) = 2,0 g
 Ethyl alcohol (95%) = 20,0 ml
Solution B
 Ammonium oxalate = 0,8 g
 Destilled water = 80,0 ml
Note : mix solutions Aand B

16. 


Iodine = 1,0 g
Potassium iodide = 2,0 g
Distilled water = 300,0 ml

17. 

Ethyl Alcohol (100%) = 95,0 ml
Distilled water = 5,0 ml

18. 


Safranin O = 0,25 ml
Ethyl alcohol (95%) = 10,0 ml
Distilled water = 100,0 ml

19. Negative Stain

20. Nigrosin, water-soluble = 10,0 g
 Disrilled water = 100,0 ml
Note : Immerse in boiling water bath for 30 minutes.
 Formalin = 0,5 ml
Note : Filter twice through double filter paper


21. Spore Stain

22. 

Malacite green = 5,0 g
Distilled water = 100,0 ml

23. 


Safranin O = 0,25 ml
Ethyl alcohol (95%) = 10,0 ml
Distilled water = 100,0 ml

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