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DNA, RNA, and Protein Synthesis http://faculty.uca.edu/~johnc/mbi1440.htm http://www.wappingersschools.org/RCK/staff/teacherhp/johnson/visualvocab/mRNA.gif
Biochemical reactions occurring in all cells are catalyzed by enzymes. ,[object Object]
Enzymes are catalyst that lower activation energy. ,[object Object],[object Object],[object Object],[object Object]
Enzymes increase the rate of the reaction at the same temperature
Enzymes are proteins. ,[object Object],[object Object],[object Object],[object Object]
Proteins – a string of amino acids SERINE – one of 20 amino acids
Proteins – a string of amino acids ,[object Object],[object Object]
Structure of DNA Hydrogen bonds Sugar-phosphate backbone Key Adenine (A) Thymine (T) Cytosine (C) Guanine (G) Section 12-1 DNA – base pairs carry the genetic code for the sequence of the amino acids Nucleotide
Nucleotides in the DNA strand carry the code for proteins ,[object Object]
  Nitrogen bases =“Steps of ladder” Purines (2 rings) Pyrimidines (1 ring) A G C T Phosphate   group Deoxyribose sugar © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved
  Nitrogen bases =“Steps of ladder” A G C T Phosphate   group Deoxyribose sugar © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved ADENINE GUANINE CYTOSINE THYMINE
CHARGAFF’S RULES ,[object Object],Image from:  http://evolution.berkeley.edu/evosite/evo101/images/dna_bases.gif _________  _________ At time no one knew why… now we know its because Adenine always bonds across with____________ Guanine always bonds across with ____________ THYMINE CYTOSINE
RNA and PROTEIN SYNTHESIS © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved DNA is safely stored in the nucleus.  mRNA (messenger RNA) brings a copy of the code out to the cytoplasm.
Transcription -   Making a copy of the DNA code  RNA DNA RNA polymerase Adenine (DNA and RNA) Cystosine (DNA and RNA) Guanine(DNA and RNA) Thymine (DNA only) Uracil (RNA only) Enzyme called RNA polymerase separates strands of DNA,  then uses one strand as a template to assemble an mRNA copy. mRNA will carry the “script” out of the nucleus to the cytoplasm
RNA- the Other Nucleic Acid ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],http://images2.clinicaltools.com/images/gene/dna_versus_rna_reversed.jpg
MASTER PLAN  DNA stays safe in nucleus   TRANSCRIPTION (DNA ->   RNA) & PROCESSING (remove interons) takes place in  nucleus TRANSLATION (RNA->   proteins) takes place on ribosomes  in  cytoplasm © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved “ Blueprints” of master plan  are carried to building site http://www.home-improvement-resource.com/images/architect.jpg
Translation -   Translating the base pair codes into an amino acid sequence mRNA  – the copy of the code sequence of base pairs tRNA  – carries the amino acid to the ribosome as coded for in the mRNA – codons code for specific amino acids.
[object Object],[object Object],[object Object],[object Object],[object Object],Images modified from © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved ANTICODON CODON EACH tRNA  carries only one kind of _____________ amino acid
HOW CAN JUST 4 BASES GIVE DIRECTIONS TO MAKE 20 AMINO ACIDS? ,[object Object],[object Object],[object Object],CODON Codons represent different amino acids Serine - Histidine - Glycine
  The m-RNA Code 64 possible codons Some amino acids have more than one codon. START= _______ 3 codons for _____ AUG STOP
 
Translation -   Translating the base pair codes into an amino acid sequence
Translation (continued)
Summary of protein synthesis DNA strand in nucleus RNA message copied from DNA  Occurs in the nucleus Occurs in the cytoplasm at the ribosome mRNA moves out to cytoplasm
A graphic to show you translation  to sequence the protein ribosome codon  carries a specific  amino acid depending upon  its code
 

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Protein synthesis

  • 1. DNA, RNA, and Protein Synthesis http://faculty.uca.edu/~johnc/mbi1440.htm http://www.wappingersschools.org/RCK/staff/teacherhp/johnson/visualvocab/mRNA.gif
  • 2.
  • 3.
  • 4. Enzymes increase the rate of the reaction at the same temperature
  • 5.
  • 6. Proteins – a string of amino acids SERINE – one of 20 amino acids
  • 7.
  • 8. Structure of DNA Hydrogen bonds Sugar-phosphate backbone Key Adenine (A) Thymine (T) Cytosine (C) Guanine (G) Section 12-1 DNA – base pairs carry the genetic code for the sequence of the amino acids Nucleotide
  • 9.
  • 10.   Nitrogen bases =“Steps of ladder” Purines (2 rings) Pyrimidines (1 ring) A G C T Phosphate group Deoxyribose sugar © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved
  • 11.   Nitrogen bases =“Steps of ladder” A G C T Phosphate group Deoxyribose sugar © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved ADENINE GUANINE CYTOSINE THYMINE
  • 12.
  • 13. RNA and PROTEIN SYNTHESIS © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved DNA is safely stored in the nucleus. mRNA (messenger RNA) brings a copy of the code out to the cytoplasm.
  • 14. Transcription - Making a copy of the DNA code RNA DNA RNA polymerase Adenine (DNA and RNA) Cystosine (DNA and RNA) Guanine(DNA and RNA) Thymine (DNA only) Uracil (RNA only) Enzyme called RNA polymerase separates strands of DNA, then uses one strand as a template to assemble an mRNA copy. mRNA will carry the “script” out of the nucleus to the cytoplasm
  • 15.
  • 16. MASTER PLAN DNA stays safe in nucleus TRANSCRIPTION (DNA -> RNA) & PROCESSING (remove interons) takes place in nucleus TRANSLATION (RNA-> proteins) takes place on ribosomes in cytoplasm © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved “ Blueprints” of master plan are carried to building site http://www.home-improvement-resource.com/images/architect.jpg
  • 17. Translation - Translating the base pair codes into an amino acid sequence mRNA – the copy of the code sequence of base pairs tRNA – carries the amino acid to the ribosome as coded for in the mRNA – codons code for specific amino acids.
  • 18.
  • 19.
  • 20.   The m-RNA Code 64 possible codons Some amino acids have more than one codon. START= _______ 3 codons for _____ AUG STOP
  • 21.  
  • 22. Translation - Translating the base pair codes into an amino acid sequence
  • 24. Summary of protein synthesis DNA strand in nucleus RNA message copied from DNA Occurs in the nucleus Occurs in the cytoplasm at the ribosome mRNA moves out to cytoplasm
  • 25. A graphic to show you translation to sequence the protein ribosome codon carries a specific amino acid depending upon its code
  • 26.