Types of Bioreactor

1. DIFFERENT TYPES OF BIOREACTER
Amjad Khan Afridi

2. Amjad Khan Afridi
DIFFERENT TYPES OF BIOREACTER

3. Mixing the media & others contents
Separation of Media and Microbes
Maintain Temperature
Remove gas and bubbles
Sparger

5. Stirred Tank Bioreactor
 In this bioreactor air is dispersed
by mechanical agitation
(impellers).
 Temperature, pH, amount of
dissolved oxygen and nutrient
concentration can be controlled
better with this reactor as
compared to other reactors.
 Baffle and rotating stirrer is
attached either on the top or at
the bottom.
 Impeller speed is reduced to 50-
150 rpm

6. 1. An agitation system
2. An oxygen delivery system
3. A foam control system
4. A temperature control system
5. A pH control system
6. Sampling ports
7. A cleaning and sterilization system
Basic Features

9. Air lift Bioreactor
• Compressed air is used for aeration
and mixing of the contents.
• This is one of the most suitable
bioreactor types for cultivation of
plant cells on a large scale.
• Properly mixing of Oxygen and
nutrients inside the vacume.
• In ALRs, the culture medium is kept
mixed and gassed by sparging air at
the bottom of the bioreactor equipped
with either an inner draught tube or
an external loop.
• This generates a vertically circulating
flow that improves oxygen transfer,
and levels shear forces.

12. Air lift Bioreactor
Merits:
 It provides reasonable mixing and oxygen transfer at
low shear.
 As it has no moving parts, it has less
contamination problem.
 Operating cost is lower than stirred tank reactor.
Demerit:
 Develop dead zones, due to insufficient mixing at high
cell density

13. Bubble Column Bioreactor
 This is one of the simplest bioreactor.
 It consists of a cylindrical vessel aerated at
the bottom.
 Used for production of metabolites from yeasts
 Also used for wastewater treatment.
 The air is pumped through a plate
located at the base of the bioreactor
 The plates has many openings through
which air or gas is passed in the form of
bubbles into the liquid medium contained
in the bioreactor
 Uniform maxing of the contents
 Lack of impellers

15. Advantages
 Have high volumetric (Volume) productivity and excellent
heat management.
 Better utilization of the plate area and flow distribution.
 Self regulating.
Disadvantages
 Less efficient than other bioreactors
 Does not have draft tube
 Higher catalyst consumption than the fixed bed
 Higher installation cost and difficult to design

16. Fluidized Bed Bioreactors
 Fluidized bed bioreactor is
comparable to bubble column
bioreactor except the top position
is expanded to reduce the
velocity of the fluid.
 The design of the fluidized bioreactors
is such that the solids are retained in
the reactor while the liquid flows out.
 These bioreactors are suitable for use
to carry out reactions involving fluid
suspended biocatalysts such as
immobilized enzymes, immobilized
cells, and microbial flocs.

18. Advantages:
1.Uniform particle mixing.
2.Uniform temperature
gradient.
3.Ability to operate reactor in
continuous state.
Dis-advantages:
1.Increased reactor vessel size.
2.Pumping requirement.
3. Pressure drop.

20. 16th June, 2021