organogenesis and it's importance

1. Topic: Organogenesis & it’s
Importance

2. Organogenesis
• Definition
“The ability of non-meristematic plant tissues to
form various organs de-novo is termed as
organogenesis.”
OR
“From cell of tissue culture various organs such
as roots, stem, leaves or flower may be initiated.
This is called organogenesis.”
• The formation of adventitious organs.
• The production of shoot, root and leaves.

3. Organs are formed in two stages:
Dedifferentiation
Redifferentiation

5. Organogenesis via Shoot Organ
Culture

6. Organogenesis via Root Organ Culture

7. Types of Organogenesis
Following types are there:
Caulogenesis-Adventitious
shoot bud initiation takes
place.
Rhizogenesis-Adventitious
root formation takes place.
Organoids
Meristemoids
Cytodifferentiation

9. Protocols for organogenesis in
tobacco callus

10. Role of PGR’s in Organogenesis
 Primary Morphogens (Agents forming
structure).
 When cells treated with these chemicals
they develop changes.
 Adopt specific developmental pathway.
 PO4 and tyrosine induce bud formation.
 Polyamine, Zeatin induce shoot bud
initiation.

11. Hormones in Organogenesis
Phytohormones
• Influence In-vitro organogenesis process.
• Skoog and Miller in 1957 said that:
“Relation concentration ratio of auxin and gibberlin
decide specific organ formation.”
• Help to regenerate dicot plants.
• High 2,4 D concentration in monocot cause
changes in development of cultured tissue.
• ABA & GA’s has role also.
• Ethylene retard organ initiation during early
stages of culture but in later help shoot initiation.

13. Factor affecting the organogenesis
• Size of ex-plant
• Source of ex-plant
• Age of ex-plant
• Seasonal variation
• Oxygen (O2 )
gradient
• Quality &
Intensity of light
• Temperature
• Culture medium
• pH of the medium
• Age of culture

14. Importance of organogenesis

15. Thank you