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AIM-STUDY OF COMPOUND MICROSCOPE
OBJECTIVE:-
TO USE A COMPOUND MICROSCOPE TO EXAMINE
VARIOUS HUMAN TISSUES AND CELLS TO GAIN A
BETTER UNDERSTANDING OF THEIR STRUCTURE.
MATERIALS:-
1.COMPOUND MICROSCOPE
2.PREPARED SLIDES OF HUMAN TISSUES (E.G.,
EPITHELIAL 3.TISSUE, CONNECTIVE TISSUE,
MUSCLE TISSUE, NERVOUS TISSUE)
4.LENS CLEANING PAPER OR SOLUTION
5.LAB NOTEBOOK
6.MICROSCOPE SLIDES AND COVERSLIPS
7.MICROSCOPE SLIDE PREPARATION KIT (IF
NEEDED)
• PROCEDURE:-
1.INTRODUCTION TO THE MICROSCOPE:-
1.BEGIN BY INTRODUCING THE VARIOUS PARTS OF THE
COMPOUND MICROSCOPE TO THE STUDENTS. DISCUSS
THE FOLLOWING COMPONENTS:
1.EYEPIECE (OCULAR LENS)
2.OBJECTIVE LENSES (TYPICALLY 4X, 10X, 40X, AND
SOMETIMES 100X)
3.COARSE AND FINE FOCUS KNOBS
4.STAGE
5.MECHANICAL STAGE CONTROLS (IF AVAILABLE)
6.DIAPHRAGM
7.CONDENSER
2.MICROSCOPE SETUP:-
• PLACE THE MICROSCOPE ON A CLEAN, STABLE SURFACE.
• ENSURE THAT THE POWER CORD OF THE MICROSCOPE'S
LIGHT SOURCE IS PLUGGED IN AND TURNED ON.
• ADJUST THE DIAPHRAGM TO CONTROL THE AMOUNT OF
LIGHT PASSING THROUGH THE STAGE.
3.PREPARING MICROSCOPE SLIDES:-
• IF NOT PROVIDED, PREPARE MICROSCOPE SLIDES WITH
SPECIMENS. THIS MAY INVOLVE:
• OBTAINING SMALL SAMPLES OF VARIOUS MATERIALS
(E.G., PLANT CELLS, ONION SKIN, A SMALL INSECT
WING).
• MOUNTING THE SPECIMENS ON SLIDES WITH
4.MICROSCOPE USAGE:-
• PLACE ONE OF THE PREPARED SLIDES ON THE STAGE OF
THE MICROSCOPE.
• SECURE THE SLIDE WITH THE MECHANICAL STAGE
CONTROLS, IF AVAILABLE.
• START WITH THE LOWEST MAGNIFICATION OBJECTIVE LENS
(TYPICALLY 4X) IN PLACE.
• LOOK THROUGH THE EYEPIECE AND USE THE COARSE
FOCUS KNOB TO BRING THE SPECIMEN INTO ROUGH
FOCUS.
• THEN, USE THE FINE FOCUS KNOB TO BRING IT INTO CLEAR
FOCUS.
5.OBSERVATION AND EXPERIMENT:-
• EXAMINE THE PREPARED SLIDES, FOCUSING ON DIFFERENT
PARTS OF THE SPECIMENS.
• RECORD YOUR OBSERVATIONS IN YOUR LAB NOTEBOOK OR
WORKSHEET. INCLUDE DETAILS ABOUT THE STRUCTURES YOU
OBSERVE.
• IF THERE ARE DIFFERENT SPECIMENS ON SLIDES, COMPARE
AND CONTRAST THEM UNDER THE MICROSCOPE.
6.DISCUSSION AND ANALYSIS:-
• DISCUSS WHAT YOU OBSERVED IN TERMS OF MAGNIFICATION
AND CLARITY.
• CONSIDER HOW VARIATIONS IN MAGNIFICATION IMPACT YOUR
ABILITY TO OBSERVE DIFFERENT STRUCTURES.
• RELATE THE STRUCTURES YOU OBSERVED TO THEIR
7.CLEANING AND SHUTDOWN:-
• AFTER USE, CAREFULLY REMOVE THE SLIDE AND
COVERSLIP.
• TURN OFF THE LIGHT SOURCE AND UNPLUG OR
SWITCH OFF THE MICROSCOPE.
• USE LENS CLEANING PAPER OR SOLUTION TO
CLEAN THE LENSES IF NECESSARY.
• STORE THE MICROSCOPE IN ITS DESIGNATED
PLACE.
SAFETY CONSIDERATIONS:-
• HANDLE THE MICROSCOPE AND GLASS SLIDES WITH CARE
TO PREVENT BREAKAGE.
• DO NOT TOUCH THE LENSES WITH YOUR FINGERS.
• FOLLOW LABORATORY SAFETY GUIDELINES AND WEAR
APPROPRIATE PROTECTIVE GEAR IF REQUIRED.
• THIS EXPERIMENT IS DESIGNED TO PROVIDE HANDS-ON
EXPERIENCE WITH A COMPOUND MICROSCOPE AND HELP
STUDENTS UNDERSTAND ITS COMPONENTS AND HOW TO
USE IT EFFECTIVELY FOR OBSERVING MICROSCOPIC
SPECIMENS.

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Study of compound microscope(BP107P).pptx

  • 1. AIM-STUDY OF COMPOUND MICROSCOPE OBJECTIVE:- TO USE A COMPOUND MICROSCOPE TO EXAMINE VARIOUS HUMAN TISSUES AND CELLS TO GAIN A BETTER UNDERSTANDING OF THEIR STRUCTURE. MATERIALS:- 1.COMPOUND MICROSCOPE 2.PREPARED SLIDES OF HUMAN TISSUES (E.G., EPITHELIAL 3.TISSUE, CONNECTIVE TISSUE, MUSCLE TISSUE, NERVOUS TISSUE) 4.LENS CLEANING PAPER OR SOLUTION 5.LAB NOTEBOOK 6.MICROSCOPE SLIDES AND COVERSLIPS 7.MICROSCOPE SLIDE PREPARATION KIT (IF NEEDED)
  • 2. • PROCEDURE:- 1.INTRODUCTION TO THE MICROSCOPE:- 1.BEGIN BY INTRODUCING THE VARIOUS PARTS OF THE COMPOUND MICROSCOPE TO THE STUDENTS. DISCUSS THE FOLLOWING COMPONENTS: 1.EYEPIECE (OCULAR LENS) 2.OBJECTIVE LENSES (TYPICALLY 4X, 10X, 40X, AND SOMETIMES 100X) 3.COARSE AND FINE FOCUS KNOBS 4.STAGE 5.MECHANICAL STAGE CONTROLS (IF AVAILABLE) 6.DIAPHRAGM 7.CONDENSER
  • 3. 2.MICROSCOPE SETUP:- • PLACE THE MICROSCOPE ON A CLEAN, STABLE SURFACE. • ENSURE THAT THE POWER CORD OF THE MICROSCOPE'S LIGHT SOURCE IS PLUGGED IN AND TURNED ON. • ADJUST THE DIAPHRAGM TO CONTROL THE AMOUNT OF LIGHT PASSING THROUGH THE STAGE. 3.PREPARING MICROSCOPE SLIDES:- • IF NOT PROVIDED, PREPARE MICROSCOPE SLIDES WITH SPECIMENS. THIS MAY INVOLVE: • OBTAINING SMALL SAMPLES OF VARIOUS MATERIALS (E.G., PLANT CELLS, ONION SKIN, A SMALL INSECT WING). • MOUNTING THE SPECIMENS ON SLIDES WITH
  • 4. 4.MICROSCOPE USAGE:- • PLACE ONE OF THE PREPARED SLIDES ON THE STAGE OF THE MICROSCOPE. • SECURE THE SLIDE WITH THE MECHANICAL STAGE CONTROLS, IF AVAILABLE. • START WITH THE LOWEST MAGNIFICATION OBJECTIVE LENS (TYPICALLY 4X) IN PLACE. • LOOK THROUGH THE EYEPIECE AND USE THE COARSE FOCUS KNOB TO BRING THE SPECIMEN INTO ROUGH FOCUS. • THEN, USE THE FINE FOCUS KNOB TO BRING IT INTO CLEAR FOCUS.
  • 5. 5.OBSERVATION AND EXPERIMENT:- • EXAMINE THE PREPARED SLIDES, FOCUSING ON DIFFERENT PARTS OF THE SPECIMENS. • RECORD YOUR OBSERVATIONS IN YOUR LAB NOTEBOOK OR WORKSHEET. INCLUDE DETAILS ABOUT THE STRUCTURES YOU OBSERVE. • IF THERE ARE DIFFERENT SPECIMENS ON SLIDES, COMPARE AND CONTRAST THEM UNDER THE MICROSCOPE. 6.DISCUSSION AND ANALYSIS:- • DISCUSS WHAT YOU OBSERVED IN TERMS OF MAGNIFICATION AND CLARITY. • CONSIDER HOW VARIATIONS IN MAGNIFICATION IMPACT YOUR ABILITY TO OBSERVE DIFFERENT STRUCTURES. • RELATE THE STRUCTURES YOU OBSERVED TO THEIR
  • 6. 7.CLEANING AND SHUTDOWN:- • AFTER USE, CAREFULLY REMOVE THE SLIDE AND COVERSLIP. • TURN OFF THE LIGHT SOURCE AND UNPLUG OR SWITCH OFF THE MICROSCOPE. • USE LENS CLEANING PAPER OR SOLUTION TO CLEAN THE LENSES IF NECESSARY. • STORE THE MICROSCOPE IN ITS DESIGNATED PLACE.
  • 7. SAFETY CONSIDERATIONS:- • HANDLE THE MICROSCOPE AND GLASS SLIDES WITH CARE TO PREVENT BREAKAGE. • DO NOT TOUCH THE LENSES WITH YOUR FINGERS. • FOLLOW LABORATORY SAFETY GUIDELINES AND WEAR APPROPRIATE PROTECTIVE GEAR IF REQUIRED. • THIS EXPERIMENT IS DESIGNED TO PROVIDE HANDS-ON EXPERIENCE WITH A COMPOUND MICROSCOPE AND HELP STUDENTS UNDERSTAND ITS COMPONENTS AND HOW TO USE IT EFFECTIVELY FOR OBSERVING MICROSCOPIC SPECIMENS.