Study of compound microscope(BP107P).pptx
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The study of a compound microscope involves understanding its components, how it works, and how to use it effectively for observing microscopic specimens. Here is a detailed description of the key aspects of a compound microscope.
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Microscopy for Microbiology – Use and FunctionHands-On L.docx
Microscopy for
Microbiology – Use and
Function
Hands-On Labs, Inc.
Version 42-0249-00-02
Review the safety materials and wear goggles when
working with chemicals. Read the entire exercise
before you begin. Take time to organize the materials
you will need and set aside a safe work space in
which to complete the exercise.
Experiment Summary:
You will identify the components of an optical microscope.
You will describe field of view and depth of field. You
will view a series of preserved specimens including
protozoans, fungi, and bacteria. You will prepare wet
mounts of cheek cells and dental tartar.
EXPERIMENT
© Hands-On Labs, Inc. www.HOLscience.com 1
Learning Objectives
Upon completion of this laboratory, you will be able to:
● Outline the components of an optical microscope.
● Describe field of view and depth of field.
● Calculate the total magnification and field of view for the lenses of an optical microscope.
● Examine prepared slides under scanning, low, high, and oil immersion lenses.
● Prepare wet mounts of cheek cells and dental plaque and examine them under different
lenses.
Time Allocation: 3.5 hours
www.HOLscience.com 2 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
www.HOLscience.com 3 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
Materials
Student Supplied Materials
Quantity Item Description
1 Camera, digital or smartphone
1 Distilled water
1 Roll of paper towels
HOL Supplied Materials
Quantity Item Description
2 Blank slides
1 Pair of gloves
1 Lens paper (50 sheets)
1 Long thin stem pipet
1 Metric ruler
1 Safety goggles
1 Slide Box:
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1 – Slide – Bacteria bacillus form
1 – Slide – Bacteria coccus form
1 – Slide – Bacteria spirillum
1 – Slide – Letter e focusing slide
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1 – Slide – Yeast, whole mount
1 Slide cover glass cube
1 Sterile swabs, 2 per pack
1 Student microscope with 100x oil immersion lens*
1 - Immersion Oil
*Microscopes and oil immersion lens are purchased separately from the LabPaq kit.
Note: To fully and accurately complete all lab exercises, you will need access to:
1. A computer to upload digital camera images.
2. Basic photo editing software, such as Microsoft Word® or PowerPoint®, to add labels, leader
lines, or text to digital photos.
3. Subject-specific textbook or appropriate reference resources from lecture content or other
suggested resources.
Note: The packaging and/or materials in this LabPaq kit may differ slightly from that which is listed
above. For an exact listing of materials, refer to the Contents List included in your LabPaq kit.
www.HOLscience.com 4 ©Hands-On Labs, Inc.
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Types of Microscopes with their applications - Microbiologynote.com
https://microbiologynote.com/types-of-microscopes-with-their-applications/
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This document provides an overview of different microscopy techniques including phase contrast microscopy, dark field microscopy, interference microscopy, and polarized microscopy. It discusses the principle, components, working, uses, advantages, and limitations of each technique. Phase contrast microscopy produces high-contrast images of transparent samples by translating refractive index variations into changes in image amplitude. Dark field microscopy uses a condenser to create a hollow cone of light, allowing objects to appear bright against a dark background. Interference microscopy generates interfering beams to produce contrast based on refractive index differences. Polarized microscopy uses polarized light to evaluate anisotropic samples and identify structures like fibers and crystals.
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The document discusses microscopes and microscopy. It defines a microscope as an optical instrument used to view very small objects magnified hundreds of times. The key parts of a microscope are described including the eyepiece, objective lenses, stage, and illuminator. The history of microscope development from the 16th century to modern electron and scanning probe microscopes is summarized. Common uses of microscopy include tissue analysis, forensic investigation, ecosystem studies, and protein analysis. Advanced microscopy techniques now allow non-invasive in vivo imaging.
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This document provides an overview of the laboratory manual for a Plant Structure and Development course. It outlines the 12 labs that will take place over the semester, including topics like plant cells, meristems, vascular tissues, and secondary growth. It also provides instructions for using a microscope to examine plant tissues and create anatomical drawings of structures like stem, root, and leaf cross-sections. The goal is for students to learn to identify and differentiate plant cell and tissue types. Making detailed observational drawings is emphasized as a way to develop important skills in visual communication and problem solving.
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The document discusses the history and components of the microscope. It describes how Antony van Leeuwenhoek is credited with developing the first microscope in the 16th century and discovering bacteria and cells. The main types of microscopes are described as optical, electron, and scanning probe microscopes. Optical microscopes are further divided into simple and compound microscopes. Compound microscopes use two lenses, the objective and eyepiece lenses, to magnify specimens, while simple microscopes use only one lens. The parts of a compound microscope and how it works to produce a magnified image are also outlined.
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This document describes the parts and proper use of a compound microscope. It begins by listing the learning objectives of being able to describe the microscope, identify its parts, and explain the functions. It then explains that the optical system contains three sets of lenses: the condenser which illuminates the slide, various objective lenses which enlarge the image, and the eyepiece which provides further magnification. Other parts like the arm, base, stage, and nosepiece are also outlined. Guidelines for safe handling and focusing the microscope are provided.
Microscopy for Microbiology – Use and FunctionHands-On L.docx
Microscopy for
Microbiology – Use and
Function
Hands-On Labs, Inc.
Version 42-0249-00-02
Review the safety materials and wear goggles when
working with chemicals. Read the entire exercise
before you begin. Take time to organize the materials
you will need and set aside a safe work space in
which to complete the exercise.
Experiment Summary:
You will identify the components of an optical microscope.
You will describe field of view and depth of field. You
will view a series of preserved specimens including
protozoans, fungi, and bacteria. You will prepare wet
mounts of cheek cells and dental tartar.
EXPERIMENT
© Hands-On Labs, Inc. www.HOLscience.com 1
Learning Objectives
Upon completion of this laboratory, you will be able to:
● Outline the components of an optical microscope.
● Describe field of view and depth of field.
● Calculate the total magnification and field of view for the lenses of an optical microscope.
● Examine prepared slides under scanning, low, high, and oil immersion lenses.
● Prepare wet mounts of cheek cells and dental plaque and examine them under different
lenses.
Time Allocation: 3.5 hours
www.HOLscience.com 2 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
www.HOLscience.com 3 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
Materials
Student Supplied Materials
Quantity Item Description
1 Camera, digital or smartphone
1 Distilled water
1 Roll of paper towels
HOL Supplied Materials
Quantity Item Description
2 Blank slides
1 Pair of gloves
1 Lens paper (50 sheets)
1 Long thin stem pipet
1 Metric ruler
1 Safety goggles
1 Slide Box:
1 – Slide – Amoeba, whole mount
1 – Slide – Bacteria bacillus form
1 – Slide – Bacteria coccus form
1 – Slide – Bacteria spirillum
1 – Slide – Letter e focusing slide
1 – Slide – Penicillium with Conidia
1 – Slide – Yeast, whole mount
1 Slide cover glass cube
1 Sterile swabs, 2 per pack
1 Student microscope with 100x oil immersion lens*
1 - Immersion Oil
*Microscopes and oil immersion lens are purchased separately from the LabPaq kit.
Note: To fully and accurately complete all lab exercises, you will need access to:
1. A computer to upload digital camera images.
2. Basic photo editing software, such as Microsoft Word® or PowerPoint®, to add labels, leader
lines, or text to digital photos.
3. Subject-specific textbook or appropriate reference resources from lecture content or other
suggested resources.
Note: The packaging and/or materials in this LabPaq kit may differ slightly from that which is listed
above. For an exact listing of materials, refer to the Contents List included in your LabPaq kit.
www.HOLscience.com 4 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
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Optical Microscopes
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Microscopy and Identification of Mircobes
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B sc biotech i bpi unit 4 microscopy
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Microbiology lab 2
The document provides an overview of microscopy, including:
1) Definitions of microscopy and related terms like magnification and resolution.
2) A brief history highlighting early pioneers like van Leeuwenhoek who made important early discoveries.
3) Descriptions of common microscope types like compound, phase contrast, and electron microscopes.
4) Explanations of key microscope components like the objective lens, eyepiece, stage, and illumination methods.
Microbiology lab 2
This document provides an overview of microscopy, including:
1. It defines microscopy as using an instrument called a microscope to view objects too small to see with the naked eye.
2. It describes some key parts and types of microscopes like compound, phase contrast, dark ground, and electron microscopes.
3. It explains concepts like magnification, resolution, and aberration that are important for microscopy.
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- 1. AIM-STUDY OF COMPOUND MICROSCOPE OBJECTIVE:- TO USE A COMPOUND MICROSCOPE TO EXAMINE VARIOUS HUMAN TISSUES AND CELLS TO GAIN A BETTER UNDERSTANDING OF THEIR STRUCTURE. MATERIALS:- 1.COMPOUND MICROSCOPE 2.PREPARED SLIDES OF HUMAN TISSUES (E.G., EPITHELIAL 3.TISSUE, CONNECTIVE TISSUE, MUSCLE TISSUE, NERVOUS TISSUE) 4.LENS CLEANING PAPER OR SOLUTION 5.LAB NOTEBOOK 6.MICROSCOPE SLIDES AND COVERSLIPS 7.MICROSCOPE SLIDE PREPARATION KIT (IF NEEDED)
- 2. • PROCEDURE:- 1.INTRODUCTION TO THE MICROSCOPE:- 1.BEGIN BY INTRODUCING THE VARIOUS PARTS OF THE COMPOUND MICROSCOPE TO THE STUDENTS. DISCUSS THE FOLLOWING COMPONENTS: 1.EYEPIECE (OCULAR LENS) 2.OBJECTIVE LENSES (TYPICALLY 4X, 10X, 40X, AND SOMETIMES 100X) 3.COARSE AND FINE FOCUS KNOBS 4.STAGE 5.MECHANICAL STAGE CONTROLS (IF AVAILABLE) 6.DIAPHRAGM 7.CONDENSER
- 3. 2.MICROSCOPE SETUP:- • PLACE THE MICROSCOPE ON A CLEAN, STABLE SURFACE. • ENSURE THAT THE POWER CORD OF THE MICROSCOPE'S LIGHT SOURCE IS PLUGGED IN AND TURNED ON. • ADJUST THE DIAPHRAGM TO CONTROL THE AMOUNT OF LIGHT PASSING THROUGH THE STAGE. 3.PREPARING MICROSCOPE SLIDES:- • IF NOT PROVIDED, PREPARE MICROSCOPE SLIDES WITH SPECIMENS. THIS MAY INVOLVE: • OBTAINING SMALL SAMPLES OF VARIOUS MATERIALS (E.G., PLANT CELLS, ONION SKIN, A SMALL INSECT WING). • MOUNTING THE SPECIMENS ON SLIDES WITH
- 4. 4.MICROSCOPE USAGE:- • PLACE ONE OF THE PREPARED SLIDES ON THE STAGE OF THE MICROSCOPE. • SECURE THE SLIDE WITH THE MECHANICAL STAGE CONTROLS, IF AVAILABLE. • START WITH THE LOWEST MAGNIFICATION OBJECTIVE LENS (TYPICALLY 4X) IN PLACE. • LOOK THROUGH THE EYEPIECE AND USE THE COARSE FOCUS KNOB TO BRING THE SPECIMEN INTO ROUGH FOCUS. • THEN, USE THE FINE FOCUS KNOB TO BRING IT INTO CLEAR FOCUS.
- 5. 5.OBSERVATION AND EXPERIMENT:- • EXAMINE THE PREPARED SLIDES, FOCUSING ON DIFFERENT PARTS OF THE SPECIMENS. • RECORD YOUR OBSERVATIONS IN YOUR LAB NOTEBOOK OR WORKSHEET. INCLUDE DETAILS ABOUT THE STRUCTURES YOU OBSERVE. • IF THERE ARE DIFFERENT SPECIMENS ON SLIDES, COMPARE AND CONTRAST THEM UNDER THE MICROSCOPE. 6.DISCUSSION AND ANALYSIS:- • DISCUSS WHAT YOU OBSERVED IN TERMS OF MAGNIFICATION AND CLARITY. • CONSIDER HOW VARIATIONS IN MAGNIFICATION IMPACT YOUR ABILITY TO OBSERVE DIFFERENT STRUCTURES. • RELATE THE STRUCTURES YOU OBSERVED TO THEIR
- 6. 7.CLEANING AND SHUTDOWN:- • AFTER USE, CAREFULLY REMOVE THE SLIDE AND COVERSLIP. • TURN OFF THE LIGHT SOURCE AND UNPLUG OR SWITCH OFF THE MICROSCOPE. • USE LENS CLEANING PAPER OR SOLUTION TO CLEAN THE LENSES IF NECESSARY. • STORE THE MICROSCOPE IN ITS DESIGNATED PLACE.
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