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YBT201
RECOMBINANT DNA
TECHNOLOGY
TOPIC : DAVIS PLAQUE
METHOD
Present by,
C . Aarthi
123011356003
Msc 1 st year
Biotechnology
Submitted to,
Ms P.Mala
Assistant Professor
Department of Biotechnology
PMIST
Content
• Definition
• Principle
• Step
• Diagram
• Plaque assay
• MCQ Questions
• MCQ Answer
• Reference
Definition
• A plaque assay, sometimes referred as
viral or bacteriophage plaque assay,
embodies a method used to calculate the
number of viruses, essentially
bacteriophage present in a sample.
Principle
• The principle of plaque assay lies in its
ability to quantify viruses. In this
technique, viruses are mixed with host
cells and plated. Each virus infects a cell
and multiplies, killing the cell and
spreading to adjacent cells, resulting in a
clear zone or 'plaque' of cell death.
Steps
• Preparation of the bacterial culture.
• Preparation of the serial dilutions 10 fold
dilution in preparation of phage
suspension.
• Mixing the virus with the bacterial culture.
• Incubation 37 C, 20 min.
• Counting the plaques to calculate viral
concentration.
Plaque assay
• Plaque assay are the standard method
that have long been used to determine
the virus titer.
• It determines the number of plaque
forming units ( PFU) in a sample.
• Typically 10 fold serial dilution of the virus
stock are inoculated into each plate.
• The virus infected cells will lyse and
spread the infection to adjacent cells.
• As the infection-to-lysis cycle is repeated,
the infected cell area will form a plaque
which can be seen visually.
• The pfu/mL result represents the number
of infectious particles in the sample,
assuming that each plaque is caused by a
single infectious virus particle.
MCQ Question
1. Is plaque a virus or bacteria ?
A) Fungi B) Bacteria C ) Virus
2.What is the principle of plaque?
A) ability to quantify bacteria B) ability to
quatify virus C) ability to quantify fungi
3.How do you calculate plaque count? A )
CFU B) PFU C) FFU
4.Plaques that you observed on the agar plates
used for bacteriophage assay are produced by
A ) Series of bacterial lysis and infection by
bacteriophage B) Viral infection of bacteria C)
Lysogenic phase of viral life cycle.
5.How do you identify the present of lamda
phage through plaque assay technique?
A) Formation of cloudy B) Clear plaque C) air
freely
MCQ Answer
1. Virus
2. Ability to quantify viruses
3. PFU per ml
4. Series of bacterial lysis and infection by
bacteriophage.
5. Formation of cloudy
Reference
•
https://bio.libretexts.org/Courses/North_Caro
lina_State_University/MB352_General_Micro
biology_Laboratory_2021_(Lee)/06%3A_Micr
obial_Physiology/6.06%3A_Lab_Procedures-
_Plaque_Assay
•
https://www.biologyonline.com/dictionary/pl
aque-assay
• https://www.studysmarter.co.uk/explanations
/biology/biology-experiments/plaque-
assay/#:~:text=The%20principle%20of%20pla
que%20assay,'plaque'%20of%20cell%20death.
• https://www.sciencedirect.com/topics/medici
ne-and-dentistry/plaque-assay.
Thank you

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