Customer Review For HPT Monoclonal Antibody (STJ10181)

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The document summarizes a customer review of an HPT monoclonal antibody from St. John's Laboratory. The customer used the antibody at a 1:1000 dilution in a Western blot assay with a 1 hour incubation time and found that it gave conclusive results. St. John's Laboratory invites customers to test their products risk-free by applying for free trial samples online and then submitting a review for 30% off their next order.

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HOW DID OUR
ANTIBODY PERFORM?
CUSTOMER REVIEW: HPT Monoclonal Antibody (STJ10181)

WHAT WAS THE PROTOCOL?
Primary antibody: Product Catalog No: STJ10181.
HPT monoclonal antibody
Provider: St John's Laboratory
Dilution ratio: 1:1000
Method for validation: Western blot
Incubation time: 1 hour

WHAT DID THE CUSTOMER THINK?
Testing results were provided by a researcher at IPB
Protein Recognition and Degradation. They stated that
“This product did give conclusive results.”

“WE INVITE YOU TO TEST OUR PRODUCTS”
Our aim is to not only connect our customers
with the best product, but to also give them
the opportunity to test the product’s
performance risk free.

Contact Us: info@stjohnslabs.com
1. Choose 5 Trial Size Samples from our free sample list
2. Complete the free sample application: http://www.stjohnslabs.com/free-sample
3. Receive your antibody
4. Submit your review
5. Receive your 30% off your next order
Get Your Free Samples in 5 Easy Steps

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Heat shock proteins, as a class, are among the most highly expressed cellular proteins across all species. As their name implies, heat shock proteins protect cells when stressed by elevated temperatures. They account for 1–2% of total protein in unstressed cells. However, when cells are heated, the fraction of heat shock proteins increases to 4–6% of cellular proteins. Hsp90 (heat shock protein 90) is a chaperone protein that assists other proteins to fold properly, stabilizes proteins against heat stress, and aids in protein degradation. It is one of the most common of the heat-related proteins. The "90" comes from the fact that it weighs roughly 90 kiloDaltons. A 90 kDa protein is considered fairly large for a non-fibrous protein. To purchase this antibody use the following link: http://www.stjohnslabs.com/hsp90a-antibody-p-92714?filter_name=HSP90A

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Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. To purchase this antibody, use the following link: http://www.stjohnslabs.com/actin-b-antibody?filter_name=STJ91464

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Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. To purchase this antibody, use the following link: http://www.stjohnslabs.com/ephb4-antibody-p-65270?filter_name=STJ23550

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Immunohistochemistry Antibody Validation Report for Anti-LC3A Antibody (STJ97...

St John's Laboratory Ltd

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Anti-CHOP-http://www.stjohnslabs.com/chop-antibody-2 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-CHOP Antibody (STJ97...

St John's Laboratory Ltd

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. Anti-phospho-MLKL (S358)-http://www.stjohnslabs.com/phospho-mlkl-s358-antibody-1 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-phospho-MLKL (S358) ...

St John's Laboratory Ltd

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. Anti-ERK1-http://www.stjohnslabs.com/erk1-antibody-3 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-ERK1 Antibody (STJ97...

St John's Laboratory Ltd

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Anti-PDGFRα-http://www.stjohnslabs.com/pdgfra-antibody-2 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-PDGFRα Antibody (STJ...

St John's Laboratory Ltd

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. / Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-. / Specifically inhibited by the cowpox virus Crma protein. Anti-Caspase-1-http://www.stjohnslabs.com/caspase-1-antibody-1 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Caspase-1 Antibody (...

St John's Laboratory Ltd

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu2+-mediated low-density lipoprotein oxidation. Anti-Amyloid-β-http://www.stjohnslabs.com/amyloid-v-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Amyloid-β Antibody (...

St John's Laboratory Ltd

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release . Anti-Bcl-2-http://www.stjohnslabs.com/bcl-2-antibody-1 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Bcl-2 Antibody (STJ9...

St John's Laboratory Ltd

Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA) is a protein that in humans is encoded by the ACTA2 gene located on 10q22-q24. Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Alpha-smooth muscle actin (α-SMA) is commonly used as a marker of myofibroblast formation. Anti-α-SMA -http://www.stjohnslabs.com/a-sma-antibody-1 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-α-SMA Antibody (STJ9...

St John's Laboratory Ltd

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