Which of the following is NOT true about MALDI-TOF MS? Sample spotted on sample plate with matrix. It can sequence genomic DNA. Small ions travel faster than largeones. Computer compares spectrum to database. It measures the masses of various components using mass spectrophotometer. Which technique(s) is/are used to help identify and classify bacteria? All of the choices are correct. Biochemical tests Nucleic acid analysis Culture characteristics Microscopic examination Current classification is three-domain system based on nucleotide sequences in DNA tRNA rRNA mRNA Cytoplasmic membrane lipids of domain is composed of hydrocarbons (not fatty acids). Bacteria Eukarya Fungus Archaea What is the biochemical test for this positive reaction? The medium becomes acidic, causing apH indicator to change color. An inverted tube traps any gas that is made. Catalase Sugar fermentation Oxidase Urease What is the biochemical test for this positive reaction? The medium becomes acidic, causing apH indicator to change color. An inverted tube traps any gas that is made. Catalase Sugar fermentation Oxidase Urease Serological testing uses to detect specific molecules. antibodies microorganisms blood chemical tests A selective growth medium accentuates differences between the growing bacteria. is cultured anaerobically. allows all bacteria to grow. allows no bacteria to grow. allows only certain bacteria to grow. A group of strains that have a characteristic serological type is called a chemivar morphovars serovar biovar Which of the following is NOT true about RFLPs? It cuts DNA samples with same restriction enzyme. Different RFLPs indicate different strains. Same species have the same RFLP pattern. restriction fragment length polymorphisms Media that allow the growth of multiple types of microbes but are designed to distinguish characteristics among members of the culture are called media. differential media selective media liquid media complex media What is the biochemical test for this positive reaction? The medium becomes alkaline, causing a pH indicator to change color. Urease Oxidase Catalase Sugar fermentation Identify any of the following as a microscopic morphology-based assay. MALDI-TOF MS Colony morphology Gram staining Antibodies A group of strains that have a characteristic biochemical pattern is called a chemivar biovar serovar morphovars Strains of a given species sometimes differ in susceptibility to various types of bacteriophages. Biochemical typing Molecular typing Serological typing Phage typing.