Expand the boundaries of reversed-phase LC and traditional GC separations with UltraPerformance Convergence Chromatography and provide the ultimate solution for routine analysis while streamlining laboratory workflow. www.waters.com/upc2
MALDI-TOF mass spectrometry is a technique used to analyze proteins. It works by ionizing protein samples using a laser and then measuring the time it takes for the ions to travel through a flight tube, which allows calculating the mass-to-charge ratio. The sample is mixed with an absorbing matrix and dried on a target plate before being ionized by a laser pulse. Ions are accelerated through a flight tube and reach a detector, with lighter ions traveling faster and reaching it first. The time of flight is converted to a mass spectrum, allowing identification of proteins in the sample. MALDI-TOF provides sensitive, high-throughput protein analysis and is widely used in fields like proteomics, microbiology,
GLP (Good Laboratory Practice) is a quality system concerned with the organization and conditions under which non-clinical health and environmental safety studies are conducted. It was instituted in the US after fraudulent data was submitted by toxicology labs to the FDA. The OECD then established principles of GLP to harmonize standards internationally. The key principles cover organization and personnel, quality assurance, facilities, equipment, test systems, study conduct, reporting and record keeping. Compliance ensures the validity and integrity of study data submitted to regulatory authorities like the FDA. India has a National GLP Compliance Monitoring Authority that inspects and certifies laboratories according to OECD GLP principles.
Detection of environmental pollutant using biosensors zen mergalKari Ann Bitgue
This document discusses biosensors, including their definition, parts, types, history and applications. It provides details on the following:
1. Biosensors can detect physiological or biochemical changes and are composed of a biological detector, transducer and processing unit.
2. Current applications of biosensors include environmental monitoring of pollutants in soil, water and air, as well as uses in biomedical diagnosis and food analysis.
3. Future trends include improvements in biosensor materials, as well as increased use of DNA and nucleic acid biosensors, though regulatory guidelines will be needed to ensure safe use and disposal of biosensors.
This document discusses viral nanoparticles and their applications. It begins with an introduction to viruses and their structure. Viruses can be engineered as nanomachines through genetic engineering, bioconjugation, biomineralization, and encapsulation. Viral nanoparticles have applications in targeted drug delivery, vaccines, imaging, and plant disease management. Challenges include issues with purity, scaling up production, and structural complexity. Overall, viral nanoparticles show promise as biological nanocarriers for applications in biomedicine and agriculture due to their ability to be chemically and genetically modified to carry drugs, toxins, and targeting sequences.
This document describes procedures for isolating, quantifying, and identifying bacteriophage from samples. It discusses the eclipse and latent periods of phage growth and viral yield. Methods are provided for amplifying phages from a sample by allowing infection of a host bacteria, then isolating and counting phages using plaque assays to determine plaque forming units per ml. Plaque assays involve serial dilutions of phages, mixing with host bacteria, and observing zones of cell lysis to identify and isolate distinct phages.
Santosh K seeks a position that allows him to utilize his knowledge and skills in microbiology. He has over 8 years of experience as a microbiologist working for several pharmaceutical companies. He holds an M.Sc. in Microbiology and has experience with quality control and assurance tasks like environmental monitoring, sterility testing, validation protocols, and document review. Santosh K is proficient in laboratory equipment and software and has undergone multiple regulatory audits.
This document provides an overview of biosensors. It defines a biosensor and discusses its key elements, including the biological recognition component, transducer, and electronic system. The document outlines the history of biosensors, from early work immobilizing enzymes in the 1910s-1920s to the development of the first glucose biosensor by Clark in 1962. It also describes various types of biosensors like calorimetric, piezoelectric, electrochemical, and optical, as well as DNA-based biosensors. Applications of biosensors discussed include food analysis, medical diagnostics, environmental monitoring, and more.
MALDI-TOF mass spectrometry is a technique used to analyze proteins. It works by ionizing protein samples using a laser and then measuring the time it takes for the ions to travel through a flight tube, which allows calculating the mass-to-charge ratio. The sample is mixed with an absorbing matrix and dried on a target plate before being ionized by a laser pulse. Ions are accelerated through a flight tube and reach a detector, with lighter ions traveling faster and reaching it first. The time of flight is converted to a mass spectrum, allowing identification of proteins in the sample. MALDI-TOF provides sensitive, high-throughput protein analysis and is widely used in fields like proteomics, microbiology,
GLP (Good Laboratory Practice) is a quality system concerned with the organization and conditions under which non-clinical health and environmental safety studies are conducted. It was instituted in the US after fraudulent data was submitted by toxicology labs to the FDA. The OECD then established principles of GLP to harmonize standards internationally. The key principles cover organization and personnel, quality assurance, facilities, equipment, test systems, study conduct, reporting and record keeping. Compliance ensures the validity and integrity of study data submitted to regulatory authorities like the FDA. India has a National GLP Compliance Monitoring Authority that inspects and certifies laboratories according to OECD GLP principles.
Detection of environmental pollutant using biosensors zen mergalKari Ann Bitgue
This document discusses biosensors, including their definition, parts, types, history and applications. It provides details on the following:
1. Biosensors can detect physiological or biochemical changes and are composed of a biological detector, transducer and processing unit.
2. Current applications of biosensors include environmental monitoring of pollutants in soil, water and air, as well as uses in biomedical diagnosis and food analysis.
3. Future trends include improvements in biosensor materials, as well as increased use of DNA and nucleic acid biosensors, though regulatory guidelines will be needed to ensure safe use and disposal of biosensors.
This document discusses viral nanoparticles and their applications. It begins with an introduction to viruses and their structure. Viruses can be engineered as nanomachines through genetic engineering, bioconjugation, biomineralization, and encapsulation. Viral nanoparticles have applications in targeted drug delivery, vaccines, imaging, and plant disease management. Challenges include issues with purity, scaling up production, and structural complexity. Overall, viral nanoparticles show promise as biological nanocarriers for applications in biomedicine and agriculture due to their ability to be chemically and genetically modified to carry drugs, toxins, and targeting sequences.
This document describes procedures for isolating, quantifying, and identifying bacteriophage from samples. It discusses the eclipse and latent periods of phage growth and viral yield. Methods are provided for amplifying phages from a sample by allowing infection of a host bacteria, then isolating and counting phages using plaque assays to determine plaque forming units per ml. Plaque assays involve serial dilutions of phages, mixing with host bacteria, and observing zones of cell lysis to identify and isolate distinct phages.
Santosh K seeks a position that allows him to utilize his knowledge and skills in microbiology. He has over 8 years of experience as a microbiologist working for several pharmaceutical companies. He holds an M.Sc. in Microbiology and has experience with quality control and assurance tasks like environmental monitoring, sterility testing, validation protocols, and document review. Santosh K is proficient in laboratory equipment and software and has undergone multiple regulatory audits.
This document provides an overview of biosensors. It defines a biosensor and discusses its key elements, including the biological recognition component, transducer, and electronic system. The document outlines the history of biosensors, from early work immobilizing enzymes in the 1910s-1920s to the development of the first glucose biosensor by Clark in 1962. It also describes various types of biosensors like calorimetric, piezoelectric, electrochemical, and optical, as well as DNA-based biosensors. Applications of biosensors discussed include food analysis, medical diagnostics, environmental monitoring, and more.
This document provides an outline and overview of class V viruses based on the Baltimore classification system. It begins with an introduction to virus classification and taxonomy, then describes key features of class V viruses which have negative-sense single-stranded RNA genomes. Examples discussed include rhabdoviruses like rabies virus and orthomyxoviruses like influenza virus. The replication cycles, genome structures, and pathogenic mechanisms of rabies virus and influenza virus are summarized in more detail.
This document discusses virus taxonomy and classification. It provides:
1. An overview of virus classification systems, which are primarily based on phenotypic characteristics like morphology, nucleic acid type, host, and disease symptoms.
2. A history of virus naming conventions from early systems based on host names to current standardized systems like ICTV and Baltimore classifications.
3. Details on the International Committee on Taxonomy of Viruses (ICTV) which develops agreed-upon virus taxonomy, names, and classifications communicated internationally.
The revolution of nanotechnology in molecular biology gives an opportunity to detect and manipulate atoms and molecules at the molecular and cellular level.
Bioremediation uses microorganisms or plants to remove pollutants from the environment. There are two main types - in situ treats pollutants on site, while ex situ removes pollutants to off-site facilities. Examples of in situ techniques include bioventing, biosparging, and in situ biodegradation which supply oxygen and nutrients to stimulate bacteria. Ex situ methods include slurry and aqueous reactors which process contaminated materials in a contained system. Bioremediation can degrade pollutants like copper but has limitations such as environmental constraints and long treatment time.
This document provides an overview of fermentation and bioprocess technology. It begins with definitions of fermentation and discusses the basic requirements for microbial growth. It then covers topics like batch, fed-batch, and continuous fermentation processes. Different types of fermenters and components like spargers and impellers are described. The document discusses strain selection and improvement methods. It also provides examples of industrial fermentation processes like ethanol production and antibiotic production. Finally, it gives an overview of downstream processing techniques used to purify products from fermentation broth, such as centrifugation, filtration, and extraction.
The document discusses biological oxygen demand (BOD) and chemical oxygen demand (COD) which are measurements of water quality. BOD refers to the amount of dissolved oxygen needed by microorganisms to break down organic matter in water over a set period of time. Higher BOD levels mean less dissolved oxygen is available to aquatic life. BOD is impacted by temperature, sewage, nutrients, turbidity, and natural processes. COD measures the total amount of oxygen required to oxidize all organic compounds in water, and COD values are always greater than BOD. The document provides details on measuring and calculating BOD and COD levels.
This document discusses Good Laboratory Practices (GLP) regulations and microbiology laboratory practices. It provides background on how GLP regulations were developed in response to malpractice issues and aim to ensure proper management and organization of studies. The key points of GLP include resources, characterization of test items, study plans and procedures, documentation of results, and quality assurance. The document also outlines biosafety levels and practices for handling different types of microorganisms, as well as guidelines for media preparation, culture maintenance, laboratory equipment use, and safety.
This document provides metadata and contents for the book "Marine Microbiology: Ecology and Applications" by C.B. Munn. It includes information about the author, publisher, publication date and language. The contents cover topics in 10 chapters including microbes in the marine environment, methods in marine microbiology, structure and physiology of marine prokaryotes, marine bacteria and archaea, marine eukaryotic microbes, marine viruses, the role of microbes in ocean processes, and symbiotic associations.
MALDI-TOF is a soft ionization technique used in mass spectrometry to analyze biomolecules like proteins, peptides, and sugars. It works by embedding analyte molecules in a crystalline matrix and using a laser to ionize the mixture, after which the ions are accelerated into a flight tube based on their mass-to-charge ratio. The time it takes ions to reach a detector is used to determine the mass of each analyte. Key advantages are its ability to analyze intact biomolecules, broad mass range, simple operation, and high sensitivity and resolution.
The ISO 17025 standard: principles and management requirements
Workshop on laboratory basics and fundamentals of ISO Quality Management Standards
March 21-22, 2018, Kyiv, Ukraine
Biosensors show the potential to complement laboratory-based analytical methods for
environmental applications. Although biosensors for potential environmental-monitoring
applications have been reported for a wide range of environmental pollutants, from a regulatory
perspective the decision to develop a biosensor method for an environmental application should
consider several interrelated issues. These issues are discussed in terms of the needs, policies,
and mechanisms associated with the identification and selection of appropriate monitoring
methods.
Molecular techniques are major tools for the analysis of microorganisms.
Molecular methods varies with respect to discriminatory power, reproducibility, ease of use, and ease of interpretation.
This ppt gives a brief review of Giant Viruses and their role in medicine?!
Mamavirus is a large and complex virus in the Group I family Mimiviridae. The virus is exceptionally large, and larger than many bacteria.
This document discusses microbial pathogens and indicators in water. It describes common waterborne pathogens like Salmonella, E. coli, Vibrio cholerae, and parasites Cryptosporidium and Giardia lamblia. Indicator bacteria like coliforms and E. coli are used to indicate fecal contamination in drinking water. Total coliforms are ubiquitous and can proliferate in distribution systems, while fecal coliforms and E. coli are more specific indicators of recent fecal contamination but are less resistant to disinfection. The document also discusses microbial indicators for recreational waters.
Biosorption uses inactive microbial biomass to bind and concentrate heavy metals from aqueous solutions, even very dilute ones. It is a promising alternative to traditional chemical precipitation for treating industrial effluents due to its low cost and high metal binding capacity. Biosorption is a metabolically passive process where heavy metals bind to functional groups on the cell surface through mechanisms like ion exchange, complexation, and chelation. Algae, fungi, bacteria, and plants have all been studied for their ability to biosorb and bioremediate heavy metals through various metabolic and non-metabolic pathways.
The document discusses various applications of nanotechnology in microbiology. It begins by defining nanotechnology as the manipulation of matter at the nanoscale of 1 to 100 nm. Some key applications discussed include using quantum dots for pathogen detection through fluorescence, using gold and silver nanoparticles in assays like sol particle immunoassays, and using magnetic nanoparticles in detection methods like magnetic relaxation switches that can detect as few as 5 viral particles. The document also discusses nanoparticle-based methods that enable faster, more sensitive detection of pathogens without sample preparation.
Biosensors integrate a biological component with a transducer to produce an electronic signal proportional to the concentration of an analyte. They have various applications including detecting glucose levels, pregnancy, infectious diseases, and hereditary conditions. Common components are a biological recognition element, transducer to convert biological response into a quantifiable signal, and detector. Examples of sensing techniques include fluorescence, SPR, impedance spectroscopy, and electrochemical methods like amperometric, potentiometric, and conductimetric. Advantages are specificity, small sample size, rapid results, and portability.
Micro chapter 31 biofilms - architects of diseaseDonna Kim
Biofilms are communities of organisms attached to a solid surface that can consist of multiple species living and interacting together. They evolve over time and are important as they represent cooperative multi-organism populations. Biofilms exist in two main types - sessile biofilms that are permanently anchored to a surface, and planktonic biofilms that are free-floating and can move to new habitats. Examples of biofilms include those found in water pipes, airplane ventilation systems, wine casks, and the lungs of cystic fibrosis patients.
There are different strategies bacterial cells use to survive. Differentiation can be occasionally one of them. Although differentiation can occur in the bacterial life cycle, it is a strategy to adapt themselves to harsh environments.
Using Fusion QbD as an Analytical Quality by Design Software for Method Devel...Waters Corporation
This presentation describes the benefits of a hardware and software platform that dramatically advances LC and LC-MS method development by applying Analytical Quality by Design (AQbD) approaches in a 100% regulatory compliance supported framework. This AQbD aligned platform includes Waters Empower™ Chromatography Data System Software with enhanced Fusion QbD® Software, the Waters® ACQUITY UPLC H-Class PLUS, a PDA detector, and QDa Mass Detector. New software capabilities that optimize and simplify the use of mass detection in the AQbD method development workflow have been added.
Visit methods.waters.com for more information
Webinar - Pharmacopeial Modernization: How Will Your Chromatography Workflow ...Waters Corporation
In this webinar, Dr. Leonel Santos and Dr. Horacio Pappa from the United States Pharmacopeia (USP) will provide an overview of its pharmacopeial harmonization and modernization efforts. The pair will also review changes described in the pending USP General Chapter <621> on liquid chromatography (LC), which will provide increased flexibility for gradient methods.
Amanda Dlugasch, from Waters Corporation, will follow with an illuminating case study, which leverages USP <621> allowable adjustments to illustrate the benefits of modernizing methods, including migrating HPLC methods to UHPLC or UPLC, without the need to revalidate.
Topics covered in this webinar will include:
- Pharmacopeial monograph modernization prioritization scheme
- Review of USP General Chapter <621> current allowable adjustments to validated chromatographic methods and forthcoming updates
- Case study on the migration of isocratic and gradient pharmacopeial methods to modern chromatography column technology, highlighting improved method performance and throughput
Replay the webinar, hosted by SelectScience:
https://www.selectscience.net/webinars/pharmacopeial-modernization-how-will-your-chromatography-workflow-benefit/?webinarID=1228
This document provides an outline and overview of class V viruses based on the Baltimore classification system. It begins with an introduction to virus classification and taxonomy, then describes key features of class V viruses which have negative-sense single-stranded RNA genomes. Examples discussed include rhabdoviruses like rabies virus and orthomyxoviruses like influenza virus. The replication cycles, genome structures, and pathogenic mechanisms of rabies virus and influenza virus are summarized in more detail.
This document discusses virus taxonomy and classification. It provides:
1. An overview of virus classification systems, which are primarily based on phenotypic characteristics like morphology, nucleic acid type, host, and disease symptoms.
2. A history of virus naming conventions from early systems based on host names to current standardized systems like ICTV and Baltimore classifications.
3. Details on the International Committee on Taxonomy of Viruses (ICTV) which develops agreed-upon virus taxonomy, names, and classifications communicated internationally.
The revolution of nanotechnology in molecular biology gives an opportunity to detect and manipulate atoms and molecules at the molecular and cellular level.
Bioremediation uses microorganisms or plants to remove pollutants from the environment. There are two main types - in situ treats pollutants on site, while ex situ removes pollutants to off-site facilities. Examples of in situ techniques include bioventing, biosparging, and in situ biodegradation which supply oxygen and nutrients to stimulate bacteria. Ex situ methods include slurry and aqueous reactors which process contaminated materials in a contained system. Bioremediation can degrade pollutants like copper but has limitations such as environmental constraints and long treatment time.
This document provides an overview of fermentation and bioprocess technology. It begins with definitions of fermentation and discusses the basic requirements for microbial growth. It then covers topics like batch, fed-batch, and continuous fermentation processes. Different types of fermenters and components like spargers and impellers are described. The document discusses strain selection and improvement methods. It also provides examples of industrial fermentation processes like ethanol production and antibiotic production. Finally, it gives an overview of downstream processing techniques used to purify products from fermentation broth, such as centrifugation, filtration, and extraction.
The document discusses biological oxygen demand (BOD) and chemical oxygen demand (COD) which are measurements of water quality. BOD refers to the amount of dissolved oxygen needed by microorganisms to break down organic matter in water over a set period of time. Higher BOD levels mean less dissolved oxygen is available to aquatic life. BOD is impacted by temperature, sewage, nutrients, turbidity, and natural processes. COD measures the total amount of oxygen required to oxidize all organic compounds in water, and COD values are always greater than BOD. The document provides details on measuring and calculating BOD and COD levels.
This document discusses Good Laboratory Practices (GLP) regulations and microbiology laboratory practices. It provides background on how GLP regulations were developed in response to malpractice issues and aim to ensure proper management and organization of studies. The key points of GLP include resources, characterization of test items, study plans and procedures, documentation of results, and quality assurance. The document also outlines biosafety levels and practices for handling different types of microorganisms, as well as guidelines for media preparation, culture maintenance, laboratory equipment use, and safety.
This document provides metadata and contents for the book "Marine Microbiology: Ecology and Applications" by C.B. Munn. It includes information about the author, publisher, publication date and language. The contents cover topics in 10 chapters including microbes in the marine environment, methods in marine microbiology, structure and physiology of marine prokaryotes, marine bacteria and archaea, marine eukaryotic microbes, marine viruses, the role of microbes in ocean processes, and symbiotic associations.
MALDI-TOF is a soft ionization technique used in mass spectrometry to analyze biomolecules like proteins, peptides, and sugars. It works by embedding analyte molecules in a crystalline matrix and using a laser to ionize the mixture, after which the ions are accelerated into a flight tube based on their mass-to-charge ratio. The time it takes ions to reach a detector is used to determine the mass of each analyte. Key advantages are its ability to analyze intact biomolecules, broad mass range, simple operation, and high sensitivity and resolution.
The ISO 17025 standard: principles and management requirements
Workshop on laboratory basics and fundamentals of ISO Quality Management Standards
March 21-22, 2018, Kyiv, Ukraine
Biosensors show the potential to complement laboratory-based analytical methods for
environmental applications. Although biosensors for potential environmental-monitoring
applications have been reported for a wide range of environmental pollutants, from a regulatory
perspective the decision to develop a biosensor method for an environmental application should
consider several interrelated issues. These issues are discussed in terms of the needs, policies,
and mechanisms associated with the identification and selection of appropriate monitoring
methods.
Molecular techniques are major tools for the analysis of microorganisms.
Molecular methods varies with respect to discriminatory power, reproducibility, ease of use, and ease of interpretation.
This ppt gives a brief review of Giant Viruses and their role in medicine?!
Mamavirus is a large and complex virus in the Group I family Mimiviridae. The virus is exceptionally large, and larger than many bacteria.
This document discusses microbial pathogens and indicators in water. It describes common waterborne pathogens like Salmonella, E. coli, Vibrio cholerae, and parasites Cryptosporidium and Giardia lamblia. Indicator bacteria like coliforms and E. coli are used to indicate fecal contamination in drinking water. Total coliforms are ubiquitous and can proliferate in distribution systems, while fecal coliforms and E. coli are more specific indicators of recent fecal contamination but are less resistant to disinfection. The document also discusses microbial indicators for recreational waters.
Biosorption uses inactive microbial biomass to bind and concentrate heavy metals from aqueous solutions, even very dilute ones. It is a promising alternative to traditional chemical precipitation for treating industrial effluents due to its low cost and high metal binding capacity. Biosorption is a metabolically passive process where heavy metals bind to functional groups on the cell surface through mechanisms like ion exchange, complexation, and chelation. Algae, fungi, bacteria, and plants have all been studied for their ability to biosorb and bioremediate heavy metals through various metabolic and non-metabolic pathways.
The document discusses various applications of nanotechnology in microbiology. It begins by defining nanotechnology as the manipulation of matter at the nanoscale of 1 to 100 nm. Some key applications discussed include using quantum dots for pathogen detection through fluorescence, using gold and silver nanoparticles in assays like sol particle immunoassays, and using magnetic nanoparticles in detection methods like magnetic relaxation switches that can detect as few as 5 viral particles. The document also discusses nanoparticle-based methods that enable faster, more sensitive detection of pathogens without sample preparation.
Biosensors integrate a biological component with a transducer to produce an electronic signal proportional to the concentration of an analyte. They have various applications including detecting glucose levels, pregnancy, infectious diseases, and hereditary conditions. Common components are a biological recognition element, transducer to convert biological response into a quantifiable signal, and detector. Examples of sensing techniques include fluorescence, SPR, impedance spectroscopy, and electrochemical methods like amperometric, potentiometric, and conductimetric. Advantages are specificity, small sample size, rapid results, and portability.
Micro chapter 31 biofilms - architects of diseaseDonna Kim
Biofilms are communities of organisms attached to a solid surface that can consist of multiple species living and interacting together. They evolve over time and are important as they represent cooperative multi-organism populations. Biofilms exist in two main types - sessile biofilms that are permanently anchored to a surface, and planktonic biofilms that are free-floating and can move to new habitats. Examples of biofilms include those found in water pipes, airplane ventilation systems, wine casks, and the lungs of cystic fibrosis patients.
There are different strategies bacterial cells use to survive. Differentiation can be occasionally one of them. Although differentiation can occur in the bacterial life cycle, it is a strategy to adapt themselves to harsh environments.
Using Fusion QbD as an Analytical Quality by Design Software for Method Devel...Waters Corporation
This presentation describes the benefits of a hardware and software platform that dramatically advances LC and LC-MS method development by applying Analytical Quality by Design (AQbD) approaches in a 100% regulatory compliance supported framework. This AQbD aligned platform includes Waters Empower™ Chromatography Data System Software with enhanced Fusion QbD® Software, the Waters® ACQUITY UPLC H-Class PLUS, a PDA detector, and QDa Mass Detector. New software capabilities that optimize and simplify the use of mass detection in the AQbD method development workflow have been added.
Visit methods.waters.com for more information
Webinar - Pharmacopeial Modernization: How Will Your Chromatography Workflow ...Waters Corporation
In this webinar, Dr. Leonel Santos and Dr. Horacio Pappa from the United States Pharmacopeia (USP) will provide an overview of its pharmacopeial harmonization and modernization efforts. The pair will also review changes described in the pending USP General Chapter <621> on liquid chromatography (LC), which will provide increased flexibility for gradient methods.
Amanda Dlugasch, from Waters Corporation, will follow with an illuminating case study, which leverages USP <621> allowable adjustments to illustrate the benefits of modernizing methods, including migrating HPLC methods to UHPLC or UPLC, without the need to revalidate.
Topics covered in this webinar will include:
- Pharmacopeial monograph modernization prioritization scheme
- Review of USP General Chapter <621> current allowable adjustments to validated chromatographic methods and forthcoming updates
- Case study on the migration of isocratic and gradient pharmacopeial methods to modern chromatography column technology, highlighting improved method performance and throughput
Replay the webinar, hosted by SelectScience:
https://www.selectscience.net/webinars/pharmacopeial-modernization-how-will-your-chromatography-workflow-benefit/?webinarID=1228
Empower 3 Chromatography Data Software (CDS) helps your entire laboratory operate better with advanced data acquisition, management, processing, and reporting that grows to meet your laboratory’s changing needs — easily scalable from a single workstation to an enterprise-wide network. In an Empower environment records are traceable so you always have full control of your data.
Waters provides compliance-ready Informatics solutions (such as Empower, UNIFI, and NuGenesis LMS) to meet the technical requirements of regulations in your industry. Since technical controls alone are not always sufficient enough to meet all regulations, Waters' Professional Services organization offers services tailored to your company's needs and can assist in meeting regulatory requirements completely.
Empower 3 Chromatography Data Software (CDS) makes it easier than ever to keep up with growing laboratory demands. When deployed as an Enterprise, it delivers value and provides significant benefits that extend beyond the workstation to your laboratory and throughout your organization. Maximize laboratory productivity with enhanced communication and system availability with Empower Enterprise.
Waters has always been committed to innovation and being at the forefront of chromatography technology, to help scientists meet the ever changing needs and challenges that they face in the lab. For example, see our constant progress in detectors.
Visualize and analyze your complex LC-MS data to support your omics research by quantifying your analytes. Find differences between samples rapidly, objectively, and reliably using multivariate statistics.
The structural elucidation of unknown compounds found in packaging is a complex and time-consuming process. Waters UNIFI Scientific Information System provides a simple workflow including scientific library creation, multivariate statistical analysis, elucidation, and reporting.
This business case examines the benefits of deploying SFC Technology at Dart NeuroScience LLC. The benefits include solvent use and waste by more than a third, a tenfold reduction in evaporation time, and sample processing time was cut by a third compared to high performance liquid chromatography (HPLC).
Large Molecule Analysis by LC-MS - Survey Infographic Waters Corporation
Sponsored by Waters, the Bioanalysis Zone Spotlight has produced an in-depth infographic on large molecule analysis by LC-MS.
This infographic displays the results of a community survey, where contributors were asked about their large molecule assays and their opinions on key issues.
Designed for the most demanding quantitative UPLC-MS/MS applications. The ultimate in tandem quadrupole performance allows you to achieve unrivalled sensitivity and robustness.
Sponsored by Waters, Bioanalysis Zone explored the analysis of antibody drug conjugates (ADCs), the unique bioanalytical challenges they pose, and how those challenges are being addressed.
The need for efficient, predictable, and connected QC lab operations has never been more important. With a trusted partner that understands the challenges and requirements, those goals can be readily achieved.
Oasis PRiME HLB infographic: How to select the best SPE method for food matricesWaters Corporation
This infographic describes how choose the most appropriate sample extraction technique for the analysis of food matrices, comparing Oasis PRiME HLB with other SPE approaches.
The document provides information on Waters CORTECS UPLC and HPLC columns including ordering details and specifications. It lists the available chemistries (C18, C18+, etc.), dimensions (2.1mm x 50mm, etc.), and part numbers. The document explains that CORTECS columns are available with 1.6um and 2.7um particles to enable methods transfer between UPLC, UHPLC, and HPLC systems for improved speed, resolution, and solvent usage. A total of 25 column configurations are available across the different chemistries and dimensions.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptxMAGOTI ERNEST
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...Sérgio Sacani
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
and the revised UWWTD (Urban Waste Water Treatment Directive)”
The binding of cosmological structures by massless topological defectsSérgio Sacani
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Immersive Learning That Works: Research Grounding and Paths ForwardLeonel Morgado
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...Travis Hills MN
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.