Using Fusion QbD as an Analytical Quality by Design Software for Method Devel...Waters Corporation
This presentation describes the benefits of a hardware and software platform that dramatically advances LC and LC-MS method development by applying Analytical Quality by Design (AQbD) approaches in a 100% regulatory compliance supported framework. This AQbD aligned platform includes Waters Empower™ Chromatography Data System Software with enhanced Fusion QbD® Software, the Waters® ACQUITY UPLC H-Class PLUS, a PDA detector, and QDa Mass Detector. New software capabilities that optimize and simplify the use of mass detection in the AQbD method development workflow have been added.
Visit methods.waters.com for more information
Webinar - Pharmacopeial Modernization: How Will Your Chromatography Workflow ...Waters Corporation
In this webinar, Dr. Leonel Santos and Dr. Horacio Pappa from the United States Pharmacopeia (USP) will provide an overview of its pharmacopeial harmonization and modernization efforts. The pair will also review changes described in the pending USP General Chapter <621> on liquid chromatography (LC), which will provide increased flexibility for gradient methods.
Amanda Dlugasch, from Waters Corporation, will follow with an illuminating case study, which leverages USP <621> allowable adjustments to illustrate the benefits of modernizing methods, including migrating HPLC methods to UHPLC or UPLC, without the need to revalidate.
Topics covered in this webinar will include:
- Pharmacopeial monograph modernization prioritization scheme
- Review of USP General Chapter <621> current allowable adjustments to validated chromatographic methods and forthcoming updates
- Case study on the migration of isocratic and gradient pharmacopeial methods to modern chromatography column technology, highlighting improved method performance and throughput
Replay the webinar, hosted by SelectScience:
https://www.selectscience.net/webinars/pharmacopeial-modernization-how-will-your-chromatography-workflow-benefit/?webinarID=1228
Empower 3 Chromatography Data Software (CDS) helps your entire laboratory operate better with advanced data acquisition, management, processing, and reporting that grows to meet your laboratory’s changing needs — easily scalable from a single workstation to an enterprise-wide network. In an Empower environment records are traceable so you always have full control of your data.
Waters provides compliance-ready Informatics solutions (such as Empower, UNIFI, and NuGenesis LMS) to meet the technical requirements of regulations in your industry. Since technical controls alone are not always sufficient enough to meet all regulations, Waters' Professional Services organization offers services tailored to your company's needs and can assist in meeting regulatory requirements completely.
Empower 3 Chromatography Data Software (CDS) makes it easier than ever to keep up with growing laboratory demands. When deployed as an Enterprise, it delivers value and provides significant benefits that extend beyond the workstation to your laboratory and throughout your organization. Maximize laboratory productivity with enhanced communication and system availability with Empower Enterprise.
Waters has always been committed to innovation and being at the forefront of chromatography technology, to help scientists meet the ever changing needs and challenges that they face in the lab. For example, see our constant progress in detectors.
Visualize and analyze your complex LC-MS data to support your omics research by quantifying your analytes. Find differences between samples rapidly, objectively, and reliably using multivariate statistics.
The structural elucidation of unknown compounds found in packaging is a complex and time-consuming process. Waters UNIFI Scientific Information System provides a simple workflow including scientific library creation, multivariate statistical analysis, elucidation, and reporting.
Using Fusion QbD as an Analytical Quality by Design Software for Method Devel...Waters Corporation
This presentation describes the benefits of a hardware and software platform that dramatically advances LC and LC-MS method development by applying Analytical Quality by Design (AQbD) approaches in a 100% regulatory compliance supported framework. This AQbD aligned platform includes Waters Empower™ Chromatography Data System Software with enhanced Fusion QbD® Software, the Waters® ACQUITY UPLC H-Class PLUS, a PDA detector, and QDa Mass Detector. New software capabilities that optimize and simplify the use of mass detection in the AQbD method development workflow have been added.
Visit methods.waters.com for more information
Webinar - Pharmacopeial Modernization: How Will Your Chromatography Workflow ...Waters Corporation
In this webinar, Dr. Leonel Santos and Dr. Horacio Pappa from the United States Pharmacopeia (USP) will provide an overview of its pharmacopeial harmonization and modernization efforts. The pair will also review changes described in the pending USP General Chapter <621> on liquid chromatography (LC), which will provide increased flexibility for gradient methods.
Amanda Dlugasch, from Waters Corporation, will follow with an illuminating case study, which leverages USP <621> allowable adjustments to illustrate the benefits of modernizing methods, including migrating HPLC methods to UHPLC or UPLC, without the need to revalidate.
Topics covered in this webinar will include:
- Pharmacopeial monograph modernization prioritization scheme
- Review of USP General Chapter <621> current allowable adjustments to validated chromatographic methods and forthcoming updates
- Case study on the migration of isocratic and gradient pharmacopeial methods to modern chromatography column technology, highlighting improved method performance and throughput
Replay the webinar, hosted by SelectScience:
https://www.selectscience.net/webinars/pharmacopeial-modernization-how-will-your-chromatography-workflow-benefit/?webinarID=1228
Empower 3 Chromatography Data Software (CDS) helps your entire laboratory operate better with advanced data acquisition, management, processing, and reporting that grows to meet your laboratory’s changing needs — easily scalable from a single workstation to an enterprise-wide network. In an Empower environment records are traceable so you always have full control of your data.
Waters provides compliance-ready Informatics solutions (such as Empower, UNIFI, and NuGenesis LMS) to meet the technical requirements of regulations in your industry. Since technical controls alone are not always sufficient enough to meet all regulations, Waters' Professional Services organization offers services tailored to your company's needs and can assist in meeting regulatory requirements completely.
Empower 3 Chromatography Data Software (CDS) makes it easier than ever to keep up with growing laboratory demands. When deployed as an Enterprise, it delivers value and provides significant benefits that extend beyond the workstation to your laboratory and throughout your organization. Maximize laboratory productivity with enhanced communication and system availability with Empower Enterprise.
Waters has always been committed to innovation and being at the forefront of chromatography technology, to help scientists meet the ever changing needs and challenges that they face in the lab. For example, see our constant progress in detectors.
Visualize and analyze your complex LC-MS data to support your omics research by quantifying your analytes. Find differences between samples rapidly, objectively, and reliably using multivariate statistics.
The structural elucidation of unknown compounds found in packaging is a complex and time-consuming process. Waters UNIFI Scientific Information System provides a simple workflow including scientific library creation, multivariate statistical analysis, elucidation, and reporting.
This business case examines the benefits of deploying SFC Technology at Dart NeuroScience LLC. The benefits include solvent use and waste by more than a third, a tenfold reduction in evaporation time, and sample processing time was cut by a third compared to high performance liquid chromatography (HPLC).
Large Molecule Analysis by LC-MS - Survey Infographic Waters Corporation
Sponsored by Waters, the Bioanalysis Zone Spotlight has produced an in-depth infographic on large molecule analysis by LC-MS.
This infographic displays the results of a community survey, where contributors were asked about their large molecule assays and their opinions on key issues.
Designed for the most demanding quantitative UPLC-MS/MS applications. The ultimate in tandem quadrupole performance allows you to achieve unrivalled sensitivity and robustness.
Sponsored by Waters, Bioanalysis Zone explored the analysis of antibody drug conjugates (ADCs), the unique bioanalytical challenges they pose, and how those challenges are being addressed.
The need for efficient, predictable, and connected QC lab operations has never been more important. With a trusted partner that understands the challenges and requirements, those goals can be readily achieved.
Oasis PRiME HLB infographic: How to select the best SPE method for food matricesWaters Corporation
This infographic describes how choose the most appropriate sample extraction technique for the analysis of food matrices, comparing Oasis PRiME HLB with other SPE approaches.
Simplifying the Screening Workflow for Forensic Toxicology InfographicWaters Corporation
With the increased use of novel psychoactive substances, the identification of compounds in a forensic toxicology investigation can be a complex and time-consuming process. Waters® UNIFI® Scientific Information System (UNIFI) provides simple workflows for targeted screening and unknown compound structural elucidation that will enable you to keep up with an ever changing illicit drug scene.
Empower® Cloud combines the world’s market leading chromatography data software (CDS), Empower, with Amazon Web Services (AWS), the world’s most secure cloud services platform, offering compute power, database storage, content delivery, and other functionality to help businesses scale and grow. Empower Cloud provides global companies the speed and agility their business requires, allowing them to focus on science and not the infrastructure.
Since Its introduction in 2004, each addition to the ACQUITY Family has continuously pushed forward to enable you to find aster, simpler, and better ways to support the work that you do.
Biopharmaceutical Attribute Monitoring with the Waters ACQUITY QDa Mass DetectorWaters Corporation
Bringing greater sensitivity, selectivity, and productivity to routine analysis of biotherapeutics, whether you're in characterization or in downstream production of biologics.
Waters: Reviewing Audit Trail Information in Empower Chromatography Data Soft...Waters Corporation
This presentation provides an overview of how to review audit trail information within Waters Empower Chromatography Data Software. (From Inform 2016, our annual software users meeting)
Learn about Waters technologies for analyzing oligonucleotides with LC-MS. We offer solutions for both oligo characterization and QC monitoring. Learn more: http://www.waters.com/oligos
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
This business case examines the benefits of deploying SFC Technology at Dart NeuroScience LLC. The benefits include solvent use and waste by more than a third, a tenfold reduction in evaporation time, and sample processing time was cut by a third compared to high performance liquid chromatography (HPLC).
Large Molecule Analysis by LC-MS - Survey Infographic Waters Corporation
Sponsored by Waters, the Bioanalysis Zone Spotlight has produced an in-depth infographic on large molecule analysis by LC-MS.
This infographic displays the results of a community survey, where contributors were asked about their large molecule assays and their opinions on key issues.
Designed for the most demanding quantitative UPLC-MS/MS applications. The ultimate in tandem quadrupole performance allows you to achieve unrivalled sensitivity and robustness.
Sponsored by Waters, Bioanalysis Zone explored the analysis of antibody drug conjugates (ADCs), the unique bioanalytical challenges they pose, and how those challenges are being addressed.
The need for efficient, predictable, and connected QC lab operations has never been more important. With a trusted partner that understands the challenges and requirements, those goals can be readily achieved.
Oasis PRiME HLB infographic: How to select the best SPE method for food matricesWaters Corporation
This infographic describes how choose the most appropriate sample extraction technique for the analysis of food matrices, comparing Oasis PRiME HLB with other SPE approaches.
Simplifying the Screening Workflow for Forensic Toxicology InfographicWaters Corporation
With the increased use of novel psychoactive substances, the identification of compounds in a forensic toxicology investigation can be a complex and time-consuming process. Waters® UNIFI® Scientific Information System (UNIFI) provides simple workflows for targeted screening and unknown compound structural elucidation that will enable you to keep up with an ever changing illicit drug scene.
Empower® Cloud combines the world’s market leading chromatography data software (CDS), Empower, with Amazon Web Services (AWS), the world’s most secure cloud services platform, offering compute power, database storage, content delivery, and other functionality to help businesses scale and grow. Empower Cloud provides global companies the speed and agility their business requires, allowing them to focus on science and not the infrastructure.
Since Its introduction in 2004, each addition to the ACQUITY Family has continuously pushed forward to enable you to find aster, simpler, and better ways to support the work that you do.
Biopharmaceutical Attribute Monitoring with the Waters ACQUITY QDa Mass DetectorWaters Corporation
Bringing greater sensitivity, selectivity, and productivity to routine analysis of biotherapeutics, whether you're in characterization or in downstream production of biologics.
Waters: Reviewing Audit Trail Information in Empower Chromatography Data Soft...Waters Corporation
This presentation provides an overview of how to review audit trail information within Waters Empower Chromatography Data Software. (From Inform 2016, our annual software users meeting)
Learn about Waters technologies for analyzing oligonucleotides with LC-MS. We offer solutions for both oligo characterization and QC monitoring. Learn more: http://www.waters.com/oligos
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
