The need for efficient, predictable, and connected QC lab operations has never been more important. With a trusted partner that understands the challenges and requirements, those goals can be readily achieved.
Using Fusion QbD as an Analytical Quality by Design Software for Method Devel...Waters Corporation
This presentation describes the benefits of a hardware and software platform that dramatically advances LC and LC-MS method development by applying Analytical Quality by Design (AQbD) approaches in a 100% regulatory compliance supported framework. This AQbD aligned platform includes Waters Empower™ Chromatography Data System Software with enhanced Fusion QbD® Software, the Waters® ACQUITY UPLC H-Class PLUS, a PDA detector, and QDa Mass Detector. New software capabilities that optimize and simplify the use of mass detection in the AQbD method development workflow have been added.
Visit methods.waters.com for more information
Webinar - Pharmacopeial Modernization: How Will Your Chromatography Workflow ...Waters Corporation
In this webinar, Dr. Leonel Santos and Dr. Horacio Pappa from the United States Pharmacopeia (USP) will provide an overview of its pharmacopeial harmonization and modernization efforts. The pair will also review changes described in the pending USP General Chapter <621> on liquid chromatography (LC), which will provide increased flexibility for gradient methods.
Amanda Dlugasch, from Waters Corporation, will follow with an illuminating case study, which leverages USP <621> allowable adjustments to illustrate the benefits of modernizing methods, including migrating HPLC methods to UHPLC or UPLC, without the need to revalidate.
Topics covered in this webinar will include:
- Pharmacopeial monograph modernization prioritization scheme
- Review of USP General Chapter <621> current allowable adjustments to validated chromatographic methods and forthcoming updates
- Case study on the migration of isocratic and gradient pharmacopeial methods to modern chromatography column technology, highlighting improved method performance and throughput
Replay the webinar, hosted by SelectScience:
https://www.selectscience.net/webinars/pharmacopeial-modernization-how-will-your-chromatography-workflow-benefit/?webinarID=1228
Empower 3 Chromatography Data Software (CDS) helps your entire laboratory operate better with advanced data acquisition, management, processing, and reporting that grows to meet your laboratory’s changing needs — easily scalable from a single workstation to an enterprise-wide network. In an Empower environment records are traceable so you always have full control of your data.
Waters provides compliance-ready Informatics solutions (such as Empower, UNIFI, and NuGenesis LMS) to meet the technical requirements of regulations in your industry. Since technical controls alone are not always sufficient enough to meet all regulations, Waters' Professional Services organization offers services tailored to your company's needs and can assist in meeting regulatory requirements completely.
Empower 3 Chromatography Data Software (CDS) makes it easier than ever to keep up with growing laboratory demands. When deployed as an Enterprise, it delivers value and provides significant benefits that extend beyond the workstation to your laboratory and throughout your organization. Maximize laboratory productivity with enhanced communication and system availability with Empower Enterprise.
Waters has always been committed to innovation and being at the forefront of chromatography technology, to help scientists meet the ever changing needs and challenges that they face in the lab. For example, see our constant progress in detectors.
Visualize and analyze your complex LC-MS data to support your omics research by quantifying your analytes. Find differences between samples rapidly, objectively, and reliably using multivariate statistics.
The structural elucidation of unknown compounds found in packaging is a complex and time-consuming process. Waters UNIFI Scientific Information System provides a simple workflow including scientific library creation, multivariate statistical analysis, elucidation, and reporting.
Using Fusion QbD as an Analytical Quality by Design Software for Method Devel...Waters Corporation
This presentation describes the benefits of a hardware and software platform that dramatically advances LC and LC-MS method development by applying Analytical Quality by Design (AQbD) approaches in a 100% regulatory compliance supported framework. This AQbD aligned platform includes Waters Empower™ Chromatography Data System Software with enhanced Fusion QbD® Software, the Waters® ACQUITY UPLC H-Class PLUS, a PDA detector, and QDa Mass Detector. New software capabilities that optimize and simplify the use of mass detection in the AQbD method development workflow have been added.
Visit methods.waters.com for more information
Webinar - Pharmacopeial Modernization: How Will Your Chromatography Workflow ...Waters Corporation
In this webinar, Dr. Leonel Santos and Dr. Horacio Pappa from the United States Pharmacopeia (USP) will provide an overview of its pharmacopeial harmonization and modernization efforts. The pair will also review changes described in the pending USP General Chapter <621> on liquid chromatography (LC), which will provide increased flexibility for gradient methods.
Amanda Dlugasch, from Waters Corporation, will follow with an illuminating case study, which leverages USP <621> allowable adjustments to illustrate the benefits of modernizing methods, including migrating HPLC methods to UHPLC or UPLC, without the need to revalidate.
Topics covered in this webinar will include:
- Pharmacopeial monograph modernization prioritization scheme
- Review of USP General Chapter <621> current allowable adjustments to validated chromatographic methods and forthcoming updates
- Case study on the migration of isocratic and gradient pharmacopeial methods to modern chromatography column technology, highlighting improved method performance and throughput
Replay the webinar, hosted by SelectScience:
https://www.selectscience.net/webinars/pharmacopeial-modernization-how-will-your-chromatography-workflow-benefit/?webinarID=1228
Empower 3 Chromatography Data Software (CDS) helps your entire laboratory operate better with advanced data acquisition, management, processing, and reporting that grows to meet your laboratory’s changing needs — easily scalable from a single workstation to an enterprise-wide network. In an Empower environment records are traceable so you always have full control of your data.
Waters provides compliance-ready Informatics solutions (such as Empower, UNIFI, and NuGenesis LMS) to meet the technical requirements of regulations in your industry. Since technical controls alone are not always sufficient enough to meet all regulations, Waters' Professional Services organization offers services tailored to your company's needs and can assist in meeting regulatory requirements completely.
Empower 3 Chromatography Data Software (CDS) makes it easier than ever to keep up with growing laboratory demands. When deployed as an Enterprise, it delivers value and provides significant benefits that extend beyond the workstation to your laboratory and throughout your organization. Maximize laboratory productivity with enhanced communication and system availability with Empower Enterprise.
Waters has always been committed to innovation and being at the forefront of chromatography technology, to help scientists meet the ever changing needs and challenges that they face in the lab. For example, see our constant progress in detectors.
Visualize and analyze your complex LC-MS data to support your omics research by quantifying your analytes. Find differences between samples rapidly, objectively, and reliably using multivariate statistics.
The structural elucidation of unknown compounds found in packaging is a complex and time-consuming process. Waters UNIFI Scientific Information System provides a simple workflow including scientific library creation, multivariate statistical analysis, elucidation, and reporting.
This business case examines the benefits of deploying SFC Technology at Dart NeuroScience LLC. The benefits include solvent use and waste by more than a third, a tenfold reduction in evaporation time, and sample processing time was cut by a third compared to high performance liquid chromatography (HPLC).
Large Molecule Analysis by LC-MS - Survey Infographic Waters Corporation
Sponsored by Waters, the Bioanalysis Zone Spotlight has produced an in-depth infographic on large molecule analysis by LC-MS.
This infographic displays the results of a community survey, where contributors were asked about their large molecule assays and their opinions on key issues.
Designed for the most demanding quantitative UPLC-MS/MS applications. The ultimate in tandem quadrupole performance allows you to achieve unrivalled sensitivity and robustness.
Sponsored by Waters, Bioanalysis Zone explored the analysis of antibody drug conjugates (ADCs), the unique bioanalytical challenges they pose, and how those challenges are being addressed.
Oasis PRiME HLB infographic: How to select the best SPE method for food matricesWaters Corporation
This infographic describes how choose the most appropriate sample extraction technique for the analysis of food matrices, comparing Oasis PRiME HLB with other SPE approaches.
Simplifying the Screening Workflow for Forensic Toxicology InfographicWaters Corporation
With the increased use of novel psychoactive substances, the identification of compounds in a forensic toxicology investigation can be a complex and time-consuming process. Waters® UNIFI® Scientific Information System (UNIFI) provides simple workflows for targeted screening and unknown compound structural elucidation that will enable you to keep up with an ever changing illicit drug scene.
Empower® Cloud combines the world’s market leading chromatography data software (CDS), Empower, with Amazon Web Services (AWS), the world’s most secure cloud services platform, offering compute power, database storage, content delivery, and other functionality to help businesses scale and grow. Empower Cloud provides global companies the speed and agility their business requires, allowing them to focus on science and not the infrastructure.
Since Its introduction in 2004, each addition to the ACQUITY Family has continuously pushed forward to enable you to find aster, simpler, and better ways to support the work that you do.
Biopharmaceutical Attribute Monitoring with the Waters ACQUITY QDa Mass DetectorWaters Corporation
Bringing greater sensitivity, selectivity, and productivity to routine analysis of biotherapeutics, whether you're in characterization or in downstream production of biologics.
Waters: Reviewing Audit Trail Information in Empower Chromatography Data Soft...Waters Corporation
This presentation provides an overview of how to review audit trail information within Waters Empower Chromatography Data Software. (From Inform 2016, our annual software users meeting)
Learn about Waters technologies for analyzing oligonucleotides with LC-MS. We offer solutions for both oligo characterization and QC monitoring. Learn more: http://www.waters.com/oligos
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
This business case examines the benefits of deploying SFC Technology at Dart NeuroScience LLC. The benefits include solvent use and waste by more than a third, a tenfold reduction in evaporation time, and sample processing time was cut by a third compared to high performance liquid chromatography (HPLC).
Large Molecule Analysis by LC-MS - Survey Infographic Waters Corporation
Sponsored by Waters, the Bioanalysis Zone Spotlight has produced an in-depth infographic on large molecule analysis by LC-MS.
This infographic displays the results of a community survey, where contributors were asked about their large molecule assays and their opinions on key issues.
Designed for the most demanding quantitative UPLC-MS/MS applications. The ultimate in tandem quadrupole performance allows you to achieve unrivalled sensitivity and robustness.
Sponsored by Waters, Bioanalysis Zone explored the analysis of antibody drug conjugates (ADCs), the unique bioanalytical challenges they pose, and how those challenges are being addressed.
Oasis PRiME HLB infographic: How to select the best SPE method for food matricesWaters Corporation
This infographic describes how choose the most appropriate sample extraction technique for the analysis of food matrices, comparing Oasis PRiME HLB with other SPE approaches.
Simplifying the Screening Workflow for Forensic Toxicology InfographicWaters Corporation
With the increased use of novel psychoactive substances, the identification of compounds in a forensic toxicology investigation can be a complex and time-consuming process. Waters® UNIFI® Scientific Information System (UNIFI) provides simple workflows for targeted screening and unknown compound structural elucidation that will enable you to keep up with an ever changing illicit drug scene.
Empower® Cloud combines the world’s market leading chromatography data software (CDS), Empower, with Amazon Web Services (AWS), the world’s most secure cloud services platform, offering compute power, database storage, content delivery, and other functionality to help businesses scale and grow. Empower Cloud provides global companies the speed and agility their business requires, allowing them to focus on science and not the infrastructure.
Since Its introduction in 2004, each addition to the ACQUITY Family has continuously pushed forward to enable you to find aster, simpler, and better ways to support the work that you do.
Biopharmaceutical Attribute Monitoring with the Waters ACQUITY QDa Mass DetectorWaters Corporation
Bringing greater sensitivity, selectivity, and productivity to routine analysis of biotherapeutics, whether you're in characterization or in downstream production of biologics.
Waters: Reviewing Audit Trail Information in Empower Chromatography Data Soft...Waters Corporation
This presentation provides an overview of how to review audit trail information within Waters Empower Chromatography Data Software. (From Inform 2016, our annual software users meeting)
Learn about Waters technologies for analyzing oligonucleotides with LC-MS. We offer solutions for both oligo characterization and QC monitoring. Learn more: http://www.waters.com/oligos
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.