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Use of western blot technique to identify inmunogenic proteins of borrelia burgdorferi for developing Lyme diseases vaccine
1. BY: CAROLINA LEMOS HERNANDEZ
3ER SEMESTRE
UNIVERSIDAD PONTIFICIA BOLIVARIANA
2. BORRELIA BURGDORFERI
Is a specie of bacterium of the
spirochaetes class and the genus
called borrelia.
Is the responsible agent of Lyme
disease
3. Lyme disease vaccine
VLA15 is a multivalent protein
subunit vaccine that targets the
outer surface protein A (OspA) of
Borrelia.
Design to protect north americans
and Europeans of LD
4. "This study was designed to determine common
reactive antigens involved in Borrelia burgdorferi
(Bb) infection that are recognized in
mammalian sera that may be useful for vaccine
development."
5. Colorimetric technique used to quantify proteins.
It is based on the variation of absorbance produced
by the color change of the Coomassie Brilliant Blue
G-250
It is used to detect a specific protein in a tissue or a
blood sample.
Is based on take a piece of the sample and put on a
special membrane, then, probe an antibody to detect
that specific protein, so in that way we can detect how
big is it and how much protein is on the sample
• H T T P S : / / E S . W I K I P E D I A . O R G / W I K I / M É T O D O _ D E _ B R A D F O R D .
• H T T P S : / / W W W . G E N O M E . G O V / G E N E T I C S - G L O S S A R Y / W E S T E R N - B L O T
6. This test help to determine specific antibodies
related to a specific infection.
the background is to use antibodies linked to
an enzime to detect and measure in a solute
A term use to called the samples where was
take the study.
At this case, blood samples.
•
10. Techniques of inmunereactivity are helpful for determine
pharmacological treatment for some infections that
could be very dangerous for the body
In medicine, molecular biology has de responsibility to
help for diseases prevention, testing so many times the
same molecule to find the variability that it has en in
human body.