Enzyme Linked Immunoabsorbent Assay

1. MOLECULAR BIOLOGY
TECHNIQUES
(ELISA)
BY
DIANA SOFÍA SERNA CORREDOR
THIRD SEMESTER
MEDICINE
UPB
2016

4. INTRODUCTION
ELISA is a trustworthy mehtod for
antigen or antibody presence
evaluation.
ELISA Constitutes an evidence for
immunological interactions

5. SEROLOGICAL EVALUATION OF
IMMUNITY TO THE VARICELLA
ZOSTER VIRUS BASED ON A
NOVEL COMPETITIVE
February 6, 2016.

6. VZV express 9 proteins
and many of these are
immunogenic.
VZV( Varicella Zoster Virus)

7. Investigation made
possible the identification
of specific mAb for each
virus protein.
*

8. 1. Detection mode: Addition of antibodies to ELISA plates to
capture recombinant virus protein
2. Use of labelled neutralized mAbs to block serum antibodies
3. Analysis. Blocking rate indicates neutralizing antibody on serum

9. I think it is a great begininig because the
results of these techniques will allow the
development of strategies that improve
treatment of viral infections, immunization
ways and identification of most expressed
virus proteins and which antibodies are
responsible to protect us against patogens.
Thanks to the studies realized was
established a new alternative
method that open the door for new
investigations about the
interactions between monoclonal
antibodies and virus proteins
STUDENT´S
OBSERVATION

10. IMMUNODIAGNOSIS OF FASCIOLA
GIGANTICA INFECTION USING
MONOCLONAL ANTIBODY-BASED
SANDWICH ELISA AND
IMMUNOCHROMATOGRAPHIC ASSAY
FOR DETECTION OF CIRCULATING
CATHEPSIN L1 PROTEASE
Faculty of science, Mahidol University
January 1, 2016.

11. Parasitological diagnosis of fasciolosis is
possesses low sensitivity.
Detection of circulating parasite antigens
is thought to be a better alternative for
diagnosis of fasciolosis.

12. WHY WAS USED ELISA
METHOD?
ESPECIFITY: 100%
SENSIBILITY: 95%
ACCURACY:98.6%

13. The study was realized in
cattle and mice infected with
fasciola gigantica serum.
MoAb 4E3 and biotinylated
rabbit anti-recombinant CatL1
antibody were used due to
their high specificities.

14. I think that is interesting that
despite of the immense quantity of
efficient methods for diagnosis and
treatment of different kind of
diseases, scientists are constantly
searching to improve it. This
investigation have allowed an
efficacy increment for diagnosis of
an infection caused by a parasite,
constituting the first step to begin
its correct and fast treatment.

15. MEDICAL
UTILITY

16. Main purpose of both investigations was
to validate an accurate method to
establish the immunity state of an
individual.

17. The Implementation
of ELISA method
allows the
identification of
presence of antigens
in patients and the
establishment of how
is functioning their
immune system in
response of thereof.

18. I think this method can be used for
epidemiological investigations and
the results of these studies at the
same time will contribute to the
development of alternatives for
primary prevention such as better
immunization programs.

19. BIBLIOGRAPHY
 Jian Liu & Xiangzhong Ye. (2016). Serological Evaluation of Immunity to the Varicella-
Zoster Virus Based on a Novel Competitive Enzyme-Linked Immunosorbent Assay.
Febreo 28. 2016, de scientific reports Sitio web:
http://www.nature.com/articles/srep20577#abstract
 Anuracpreeda P, Chawengkirttikul R, Sobhon P. (2016). MEDLINE® Immunodiagnosis of
Fasciola gigantica Infection Using Monoclonal Antibody-Based Sandwich ELISA and
Immunochromatographic Assay for Detection of Circulating Cathepsin L1 Protease..
March 1, de Medline Sitio web:
https://www-clinicalkey-es.consultaremota.upb.edu.co/#!/content/medline/2-s2.0-2673140
 MARTINEZ SÁNCHEZ, Lina María. Biología molecular. 8va ed. Medellín: UPB. Fac. de
Medicina, 2015. 200,201 p.

20. Thanks.