Water is a valuable natural resource, being considered a major source for the maintenance of social and political stability. In this paper we discuss how water influences on contemporary international relations between States, taking as an example the La Plata River basin (LPRB) countries. Located in South America and covering parts of five countries (Argentina, Bolivia, Brazil, Paraguay and Uruguay), the LPRB has an area of more than 3 million km², essential not just for economics means, but for the social and political development of the region. This transboundary basin is considered to be one of the most important water reservoirs in the world and has been the scene of conflicts since the beginning of the colonization period, influencing even in the present international relations. The objective of this paper is, firstly, to present how water issues interact with international relations, especially due to cooperation and conflict processes. Second, to present the LPRB and discuss the states international relations based on their legislations, transboundary issues and cooperation processes. This work will contribute to water management, especially regarding transboundary water, showing that water is a powerful force and play a major role in the international relations, being able to affect countries and societies.