This document discusses vitamin B1 (thiamine). It provides information on the structure of thiamine, dietary sources, roles in the body including as a coenzyme in important metabolic reactions, deficiency manifestations such as beriberi, and absorption/transport of thiamine in the body. The key points are that thiamine is a water-soluble vitamin required for important metabolic processes, it acts as a coenzyme (thiamine pyrophosphate) in the oxidative decarboxylation of pyruvate and alpha-ketoglutarate, and deficiency can cause diseases like beriberi if intake is inadequate.
Thiamine (vitamin B1) and biochemical aspects of beriberirohini sane
A comprehensive presentation on Thiamine and biochemical aspects of Beriberi for MBBS, BDS, B Pham and Biotechnology students to facilitate easy leaning.
Chemistry of vitamin B1, Biochemical functions of vitamin B1, Recommended dietary Allowance of Thiamine, Dietary sources of Thiamine ,Deficiency symptoms of Thiamine
This document discusses vitamin B1 (thiamine) for dogs. It provides information on the structure, functions, absorption, transport, excretion and deficiency of thiamine. Thiamine acts as a coenzyme to support carbohydrate metabolism and energy production. It is water soluble, unstable to heat and light, and not stored in the body. Sources of thiamine for dogs include spinach, eggs, fish and liver. A thiamine deficiency can cause neurological and muscular issues in dogs.
Thiamin (vitamin B1) is essential for carbohydrate metabolism and neural function. Severe deficiency causes beriberi while milder deficiency causes non-specific symptoms like malaise. Thiamin deficiency has been common in people relying mainly on polished rice. The removal of rice bran during polishing removes thiamin. The richest sources of thiamin are brewer's yeast, pork liver, and whole grains. However, thiamin is unstable when heated or exposed to oxidizing and alkaline conditions.
This document discusses vitamin B1 (thiamine). It provides information on the structure of thiamine, dietary sources, roles in the body including as a coenzyme in important metabolic reactions, deficiency manifestations such as beriberi, and absorption/transport of thiamine in the body. The key points are that thiamine is a water-soluble vitamin required for important metabolic processes, it acts as a coenzyme (thiamine pyrophosphate) in the oxidative decarboxylation of pyruvate and alpha-ketoglutarate, and deficiency can cause diseases like beriberi if intake is inadequate.
Thiamine (vitamin B1) and biochemical aspects of beriberirohini sane
A comprehensive presentation on Thiamine and biochemical aspects of Beriberi for MBBS, BDS, B Pham and Biotechnology students to facilitate easy leaning.
Chemistry of vitamin B1, Biochemical functions of vitamin B1, Recommended dietary Allowance of Thiamine, Dietary sources of Thiamine ,Deficiency symptoms of Thiamine
This document discusses vitamin B1 (thiamine) for dogs. It provides information on the structure, functions, absorption, transport, excretion and deficiency of thiamine. Thiamine acts as a coenzyme to support carbohydrate metabolism and energy production. It is water soluble, unstable to heat and light, and not stored in the body. Sources of thiamine for dogs include spinach, eggs, fish and liver. A thiamine deficiency can cause neurological and muscular issues in dogs.
Thiamin (vitamin B1) is essential for carbohydrate metabolism and neural function. Severe deficiency causes beriberi while milder deficiency causes non-specific symptoms like malaise. Thiamin deficiency has been common in people relying mainly on polished rice. The removal of rice bran during polishing removes thiamin. The richest sources of thiamin are brewer's yeast, pork liver, and whole grains. However, thiamin is unstable when heated or exposed to oxidizing and alkaline conditions.
LIBYAN INTERNATIONAL
MEDICAL UNIVERSITY
students : Mohammed Elgheriyani , Mohammed Moaiter
Mohamme Amsadef , Mohammed Alsanousi ,
Ali Hassen
1- Structure & forms
Vitamin B1
2- Biochemical role & functions
of Vitamin B1
3- Causes of Vitamin B1 deficiency
4- Clinical Manifestation of vitamin B1 deficiency
This document discusses vitamin B1 (thiamine). It notes that thiamine is a water-soluble vitamin that acts as a coenzyme involved in carbohydrate metabolism. The key points are:
- Thiamine's coenzyme, thiamine pyrophosphate, facilitates the conversion of pyruvate to acetyl-CoA and links glycolysis to the TCA cycle.
- Deficiency can cause beriberi disease, characterized by neuropathy, edema, and heart failure.
- Good dietary sources include cereals, legumes, nuts and meat. Cooking can reduce thiamine levels in foods.
Thiamin, also known as vitamin B1, is a water-soluble vitamin that is involved in many chemical reactions in the body. Common food sources of thiamin include whole grains, meat, fish, and fortified breads and cereals. Thiamin deficiency can cause a disease called beriberi and results in weakness and impairment of nervous, muscle, gastrointestinal and cardiovascular systems. While thiamin deficiency is now rare in developed countries, those at highest risk include individuals who abuse alcohol, have certain gastrointestinal diseases, or eating disorders.
This document discusses the water soluble vitamin thiamine (vitamin B1). It is involved in carbohydrate, fat, and amino acid metabolism. Sources include cereals, liver, meat and eggs. Requirements increase during times of stress. A deficiency can cause beriberi with cardiovascular and neurological symptoms. Treatment involves supplemental thiamine.
This document discusses several B vitamins and vitamin A, including their sources, roles, and effects of deficiency and excess. Vitamin B1 deficiency can cause beriberi or Wernicke-Korsakoff syndrome. Vitamin B6 deficiency results in convulsions and hyperirritability. Vitamin B12 deficiency leads to subacute combined degeneration of the spinal cord. Vitamin A deficiency causes blindness, while excess causes tissue damage and central nervous system toxicity.
This document summarizes information about vitamin B1 (thiamine) and its active coenzyme form, thiamine pyrophosphate (TPP). It discusses the history of thiamine discovery and its chemical structure. Thiamine is converted to TPP in the liver and intestinal mucosa by the enzyme thiamine pyrophosphokinase using ATP. TPP acts as a coenzyme, transferring aldehyde groups in metabolic reactions. Sources of thiamine are mentioned and a deficiency can cause beriberi. Functions include roles in growth, nervous system maintenance, and metabolism.
Absorption, transport and metabolism of thiaminDomina Petric
Thiamin is absorbed in the small intestine through both active transport and passive diffusion. It is carried in the blood bound to proteins and taken up by tissues, where it is phosphorylated. The majority of thiamin in tissues is in phosphorylated form. Thiamin is metabolized through phosphorylation and dephosphorylation. Excess thiamin is rapidly excreted in the urine, with the half-life being 10-20 days. Deficiency can deplete tissue stores within a couple weeks.
Healing takes energy. Energy requires thiamine. Modern foods, lifestyles, medications and chemical exposures threaten thiamine status in a large percentage of the population. Modern thiamine deficiency does not look like classic thiamine deficiency, and thus, goes largely unrecognized by the medical community. Modern thiamine deficiency does not originate from starvation, but rather, from a state of being well fed and sometimes over-fed. As a result, many of the symptoms are incongruent with current textbook definitions. This presentation discusses the chemistry and symptomology of modern thiamine deficiency.
This document summarizes key aspects of several water-soluble vitamins, including their functions, absorption, clinical deficiencies, food sources, and uses for supplementation. It discusses the vitamins thiamine, riboflavin, niacin, pyridoxine, folic acid, and vitamin C, describing how each acts as a coenzyme in important metabolic processes and what health issues can result from deficiencies. Food sources rich in each vitamin are also outlined, as well as therapeutic uses for supplementation.
Thiamine pyrophosphate is the biologically active form of the vitamin, formed by the transfer of a pyrophosphate group (PPi) from adenosine triphosphate (ATP) to thiamine
ATP → AMP + PPi
TPP serves as a coenzyme (a non-protein compound that is necessary for the functioning of the enzyme)
In the formation or degradation of α-ketols by transketolase (A)
In the oxidative decarboxylation of α-keto acids (B)
ATP
α-ketoglutarate Dehydrogenase
Pyruvate Dehydrogenase
This document discusses four disorders of amino acid metabolism:
1. Tyrosinemia is caused by a defect in the breakdown of tyrosine, leading to its buildup. There are three types caused by different enzyme deficiencies. Symptoms vary but can include liver and kidney failure.
2. Maple syrup urine disease results from a defect in breaking down branched chain amino acids. Affected individuals cannot metabolize certain amino acids, causing neurologic issues and a maple syrup smelling urine.
3. Homocystinuria is caused by the inability to metabolize the amino acid homocysteine. It can cause skeletal abnormalities and intellectual disabilities if left untreated.
4. Albinism is caused by a lack
Thiamine is also called as vitamin B1, Aneurine (it can relieve neuritis), or antiberberi
factor.
• In 1900, Christian Eijkman produced beriberi in chicken by feeding polished rice (Nobel
Prize, 1929).
• Adolf Windaus (Nobel Prize, 1928) elucidated the structure of the vitamin.
Structure of Thiamine:
Biosynthesis:
Deficiency Manifestations of Thiamine
Thiamine, also known as thiamin or vitamin B₁, is a vitamin found in food and manufactured as a dietary supplement and medication. Food sources of thiamine include whole grains, legumes, and some meats and fish
This document defines vitamins and classifies them as either water soluble or fat soluble. It then discusses the vitamin thiamine (vitamin B1) in detail. Thiamine acts as a coenzyme in carbohydrate metabolism and energy production. Deficiency results in beriberi disease. The document also covers the structure, functions, deficiency and food sources of riboflavin (vitamin B2).
The presentation has been uploaded by the student of ESIC Medical College, Gulbarga. This PPT is related to Vitamin B1 (Thiamine). It is focusing on the biochemical point of view. It gives detailed and understandable knowledge about Vitamin B1.
This document summarizes vitamin B1 (thiamine). It discusses the chemistry of thiamine and its coenzyme thiamine pyrophosphate, which is important for carbohydrate metabolism. A deficiency in thiamine results in beriberi disease, which has different forms including dry beriberi affecting the nervous system and wet beriberi affecting the cardiovascular system. The document outlines recommended daily intakes of thiamine, dietary sources, deficiency symptoms, and conditions caused by thiamine deficiency like Wernicke-Korsakoff syndrome in alcoholics. Thiamine deficiency can also occur due to the presence of thiaminase in some foods or pyrithiamine in plant fer
Thiamine (vitamin B1) is a water-soluble vitamin that acts as a cofactor for several enzyme complexes involved in carbohydrate metabolism. It contains pyrimidine and thiazole rings joined by a methylene bridge. Thiamine is converted to its active coenzyme form, thiamine pyrophosphate (TPP), which participates in reactions like glycolysis and the citric acid cycle. Deficiency can result in beriberi disease, characterized by neuropathy or heart failure depending on whether the dry or wet form occurs. Diagnosis involves measuring transketolase levels in red blood cells.
Thiamine, also known as vitamin B1, is an essential nutrient that plays a key role in carbohydrate metabolism. It consists of a pyrimidine ring connected to a thiazole ring. Thiamine acts as a cofactor for several enzymes involved in the breakdown of carbohydrates and the citric acid cycle. Deficiency of thiamine can cause diseases like beriberi, which presents as either "wet" beriberi with edema or "dry" beriberi with neurological symptoms. Treatment involves high doses of thiamine supplementation.
Thiamine (vitamin B1) is a water-soluble vitamin that acts as a coenzyme in carbohydrate metabolism. It contains pyrimidine and thiazole rings connected by a methylene bridge. Thiamine is converted to its active coenzyme form, thiamine pyrophosphate (TPP), which is involved in several metabolic reactions like pyruvate dehydrogenase complex and transketolase. Deficiency of thiamine causes beriberi disease characterized by peripheral neuropathy or heart failure.
PPT on Direct Seeded Rice presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
LIBYAN INTERNATIONAL
MEDICAL UNIVERSITY
students : Mohammed Elgheriyani , Mohammed Moaiter
Mohamme Amsadef , Mohammed Alsanousi ,
Ali Hassen
1- Structure & forms
Vitamin B1
2- Biochemical role & functions
of Vitamin B1
3- Causes of Vitamin B1 deficiency
4- Clinical Manifestation of vitamin B1 deficiency
This document discusses vitamin B1 (thiamine). It notes that thiamine is a water-soluble vitamin that acts as a coenzyme involved in carbohydrate metabolism. The key points are:
- Thiamine's coenzyme, thiamine pyrophosphate, facilitates the conversion of pyruvate to acetyl-CoA and links glycolysis to the TCA cycle.
- Deficiency can cause beriberi disease, characterized by neuropathy, edema, and heart failure.
- Good dietary sources include cereals, legumes, nuts and meat. Cooking can reduce thiamine levels in foods.
Thiamin, also known as vitamin B1, is a water-soluble vitamin that is involved in many chemical reactions in the body. Common food sources of thiamin include whole grains, meat, fish, and fortified breads and cereals. Thiamin deficiency can cause a disease called beriberi and results in weakness and impairment of nervous, muscle, gastrointestinal and cardiovascular systems. While thiamin deficiency is now rare in developed countries, those at highest risk include individuals who abuse alcohol, have certain gastrointestinal diseases, or eating disorders.
This document discusses the water soluble vitamin thiamine (vitamin B1). It is involved in carbohydrate, fat, and amino acid metabolism. Sources include cereals, liver, meat and eggs. Requirements increase during times of stress. A deficiency can cause beriberi with cardiovascular and neurological symptoms. Treatment involves supplemental thiamine.
This document discusses several B vitamins and vitamin A, including their sources, roles, and effects of deficiency and excess. Vitamin B1 deficiency can cause beriberi or Wernicke-Korsakoff syndrome. Vitamin B6 deficiency results in convulsions and hyperirritability. Vitamin B12 deficiency leads to subacute combined degeneration of the spinal cord. Vitamin A deficiency causes blindness, while excess causes tissue damage and central nervous system toxicity.
This document summarizes information about vitamin B1 (thiamine) and its active coenzyme form, thiamine pyrophosphate (TPP). It discusses the history of thiamine discovery and its chemical structure. Thiamine is converted to TPP in the liver and intestinal mucosa by the enzyme thiamine pyrophosphokinase using ATP. TPP acts as a coenzyme, transferring aldehyde groups in metabolic reactions. Sources of thiamine are mentioned and a deficiency can cause beriberi. Functions include roles in growth, nervous system maintenance, and metabolism.
Absorption, transport and metabolism of thiaminDomina Petric
Thiamin is absorbed in the small intestine through both active transport and passive diffusion. It is carried in the blood bound to proteins and taken up by tissues, where it is phosphorylated. The majority of thiamin in tissues is in phosphorylated form. Thiamin is metabolized through phosphorylation and dephosphorylation. Excess thiamin is rapidly excreted in the urine, with the half-life being 10-20 days. Deficiency can deplete tissue stores within a couple weeks.
Healing takes energy. Energy requires thiamine. Modern foods, lifestyles, medications and chemical exposures threaten thiamine status in a large percentage of the population. Modern thiamine deficiency does not look like classic thiamine deficiency, and thus, goes largely unrecognized by the medical community. Modern thiamine deficiency does not originate from starvation, but rather, from a state of being well fed and sometimes over-fed. As a result, many of the symptoms are incongruent with current textbook definitions. This presentation discusses the chemistry and symptomology of modern thiamine deficiency.
This document summarizes key aspects of several water-soluble vitamins, including their functions, absorption, clinical deficiencies, food sources, and uses for supplementation. It discusses the vitamins thiamine, riboflavin, niacin, pyridoxine, folic acid, and vitamin C, describing how each acts as a coenzyme in important metabolic processes and what health issues can result from deficiencies. Food sources rich in each vitamin are also outlined, as well as therapeutic uses for supplementation.
Thiamine pyrophosphate is the biologically active form of the vitamin, formed by the transfer of a pyrophosphate group (PPi) from adenosine triphosphate (ATP) to thiamine
ATP → AMP + PPi
TPP serves as a coenzyme (a non-protein compound that is necessary for the functioning of the enzyme)
In the formation or degradation of α-ketols by transketolase (A)
In the oxidative decarboxylation of α-keto acids (B)
ATP
α-ketoglutarate Dehydrogenase
Pyruvate Dehydrogenase
This document discusses four disorders of amino acid metabolism:
1. Tyrosinemia is caused by a defect in the breakdown of tyrosine, leading to its buildup. There are three types caused by different enzyme deficiencies. Symptoms vary but can include liver and kidney failure.
2. Maple syrup urine disease results from a defect in breaking down branched chain amino acids. Affected individuals cannot metabolize certain amino acids, causing neurologic issues and a maple syrup smelling urine.
3. Homocystinuria is caused by the inability to metabolize the amino acid homocysteine. It can cause skeletal abnormalities and intellectual disabilities if left untreated.
4. Albinism is caused by a lack
Thiamine is also called as vitamin B1, Aneurine (it can relieve neuritis), or antiberberi
factor.
• In 1900, Christian Eijkman produced beriberi in chicken by feeding polished rice (Nobel
Prize, 1929).
• Adolf Windaus (Nobel Prize, 1928) elucidated the structure of the vitamin.
Structure of Thiamine:
Biosynthesis:
Deficiency Manifestations of Thiamine
Thiamine, also known as thiamin or vitamin B₁, is a vitamin found in food and manufactured as a dietary supplement and medication. Food sources of thiamine include whole grains, legumes, and some meats and fish
This document defines vitamins and classifies them as either water soluble or fat soluble. It then discusses the vitamin thiamine (vitamin B1) in detail. Thiamine acts as a coenzyme in carbohydrate metabolism and energy production. Deficiency results in beriberi disease. The document also covers the structure, functions, deficiency and food sources of riboflavin (vitamin B2).
The presentation has been uploaded by the student of ESIC Medical College, Gulbarga. This PPT is related to Vitamin B1 (Thiamine). It is focusing on the biochemical point of view. It gives detailed and understandable knowledge about Vitamin B1.
This document summarizes vitamin B1 (thiamine). It discusses the chemistry of thiamine and its coenzyme thiamine pyrophosphate, which is important for carbohydrate metabolism. A deficiency in thiamine results in beriberi disease, which has different forms including dry beriberi affecting the nervous system and wet beriberi affecting the cardiovascular system. The document outlines recommended daily intakes of thiamine, dietary sources, deficiency symptoms, and conditions caused by thiamine deficiency like Wernicke-Korsakoff syndrome in alcoholics. Thiamine deficiency can also occur due to the presence of thiaminase in some foods or pyrithiamine in plant fer
Thiamine (vitamin B1) is a water-soluble vitamin that acts as a cofactor for several enzyme complexes involved in carbohydrate metabolism. It contains pyrimidine and thiazole rings joined by a methylene bridge. Thiamine is converted to its active coenzyme form, thiamine pyrophosphate (TPP), which participates in reactions like glycolysis and the citric acid cycle. Deficiency can result in beriberi disease, characterized by neuropathy or heart failure depending on whether the dry or wet form occurs. Diagnosis involves measuring transketolase levels in red blood cells.
Thiamine, also known as vitamin B1, is an essential nutrient that plays a key role in carbohydrate metabolism. It consists of a pyrimidine ring connected to a thiazole ring. Thiamine acts as a cofactor for several enzymes involved in the breakdown of carbohydrates and the citric acid cycle. Deficiency of thiamine can cause diseases like beriberi, which presents as either "wet" beriberi with edema or "dry" beriberi with neurological symptoms. Treatment involves high doses of thiamine supplementation.
Thiamine (vitamin B1) is a water-soluble vitamin that acts as a coenzyme in carbohydrate metabolism. It contains pyrimidine and thiazole rings connected by a methylene bridge. Thiamine is converted to its active coenzyme form, thiamine pyrophosphate (TPP), which is involved in several metabolic reactions like pyruvate dehydrogenase complex and transketolase. Deficiency of thiamine causes beriberi disease characterized by peripheral neuropathy or heart failure.
PPT on Direct Seeded Rice presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skills
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
The cost of acquiring information by natural selectionCarl Bergstrom
This is a short talk that I gave at the Banff International Research Station workshop on Modeling and Theory in Population Biology. The idea is to try to understand how the burden of natural selection relates to the amount of information that selection puts into the genome.
It's based on the first part of this research paper:
The cost of information acquisition by natural selection
Ryan Seamus McGee, Olivia Kosterlitz, Artem Kaznatcheev, Benjamin Kerr, Carl T. Bergstrom
bioRxiv 2022.07.02.498577; doi: https://doi.org/10.1101/2022.07.02.498577
The debris of the ‘last major merger’ is dynamically youngSérgio Sacani
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...Leonel Morgado
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
3. How does vitamins function
Vitamins form part of coenzymes that enables
enzymes to release energy from
carbohydrates, proteins and fats.
4. What is thiamine(B1)
Thiamin is a vitamin, an essential micronutrient. It
is a water-soluble vitamin, which means it
dissolves in water and is carried to different parts
of the body but is not stored in the body.
Thiamine is on the World Health Organization's
List of Essential Medicines.
Within the body, the best-characterized form
is thiamine pyrophosphate(TPP), also called
thiamine diphosphate, a coenzyme in
the catabolism of sugars and amino acids
5. Thiamine
Thiamine was first synthesized in 1926 as a coenzyme,
which was very helpful in researching its properties in
relation to beriberi, a vitamin b1 deficiency.
as the first B vitamin discovered, so was termed vitamin
B1.
Compared to other micronutrients, body stores of
thiamine are low. In addition, thiamine's half-life is only
10 to 14 days. Therefore, a continuous source of
dietary thiamine is necessary to prevent deficiency.
6. Metabolism
It is an essential part of energy metabolism. This means that
thiamine helps convert carbohydrates into energy. Eating
carbohydrates increases the need for this vitamin, as your body
can only store about 30mg at a time due to the vitamins short
half-life.
Thiamine metabolism begins in the extracellular space, being
transported by a thiamine transporter into the cell.
Once in the intracellular space, thiamine is converted into
thiamine pyrophosphate through the enzyme thiamin
pyrophosphate kinase. Thiamine pyrophosphate is then
converted into thiamine triphosphate, again using the enzyme
thiamin pyrophosphate kinase .
In the jejunum, thiamine is converted to thiamine
pyrophosphate(TPP), which is the main coenzyme form of
thiamine.
7. Metabolism
Thiamine and TPP play important roles within
mitochondria in the decarboxylation of α-keto
acids to coenzyme A (CoA) moieties, an important
process in ATP synthesis. In short conversion of
pyruvate to acetyl coA.
9. hj
The uptake of thiamine is enhanced
by thiamine deficiency, but can be reduced by
the presence of diabetes mellitus.
Additionally, thiamine absorption is inhibited
by thiamine analogs (such as metronidazole),
ethanol, and diuretics (such as amiloride).
Thiamine is mainly excreted in urine.
10. Functions
Thiamin is important for the
growth, development, and
function of the cells in the body.
TPP is a coenzyme in reactions
involved in myelin formation.
Muscles heavily depends on
thiamin.
Thiamine may also have an
effect on nerve conduction and
transmission.
Thiamine is involved in a wide
variety of the intricate
biochemical pathways
necessary for proper tissue and
organ function
11. Functions
Thiamine plays a pivotal role in the pentose
phosphate pathway, which is not only an
alternate glucose metabolism pathway, but
also a major route for the synthesis of
several neurotransmitters, nuclic acids, lipids,
amino acids, steroids, and glutathione.
12. Dietary sources
It is found in food and
commercially synthesized to
be a dietary
supplement or medication.
Food sources of thiamine
include whole
grains, legumes, and some
meats and fish. Grain
processing removes much of
the thiamine content, so in
many
countries cereals and flours a
re enriched with thiamine.
13. Deficiency
People who are malnourished, who eat polished, refined cereals
and those who derive most of their energy from empty k calorie
items, like alcohol, risk thiamine deficiency.
Prolonged thiamine deficiency can result in the disease beriberi.
Symptoms of beriberi includes damage to nervous system, the
heart and the other muscles.
It also can cause paralysis as it participates in nerve processes.
In alcoholics the most serious deficiency causes wernicke-
korsakoff. Symptoms include disorientation, loss of short term
memory, jerky eye movement, and staggering gait.
Thiamine deficiency can lead to autonomic nervous system
dysfunction, abnormal vagal tone, and lower acetylcholine
synthesis.
14.
15. References
Lehninger, A.L. Lehninger principles of biochemistry (4th ed.) (2005).
New York: W.H Freeman.
Manzetti S, Zhang J, van der Spoel D: Thiamin function, metabolism,
uptake, and transport. Biochemistry. 2014 Feb 11;53(5):821-35. doi:
10.1021/bi401618y. Epub 2014 Jan 31.
Pubmed: 24460461
Nosaka K, Onozuka M, Kakazu N, Hibi S, Nishimura H, Nishino H, Abe T:
Isolation and characterization of a human thiamine pyrophosphokinase
cDNA. Biochim Biophys Acta. 2001 Jan 26;1517(2):293-7. doi:
10.1016/s0167-4781(00)00247-5.
Pubmed: 11342111
Zhao R, Gao F, Goldman ID: Molecular cloning of human thiamin
pyrophosphokinase. Biochim Biophys Acta. 2001 Jan 26;1517(2):320-2.
doi: 10.1016/s0167-4781(00)00264-5.
Pubmed: 11342117
Editor's Notes
GABA is a neurotransmitter and acts as a chemical messanger
Beri means weakness
Berberi is of two types wet
(edema) and dry(muscle wasting and no edema)