This document discusses biofertilizers, which are beneficial microorganisms that can be used to fertilize soils and promote plant growth. It describes different types of nitrogen-fixing and phosphate-solubilizing biofertilizers including bacteria like Rhizobium, Azotobacter, and Pseudomonas. Fungi like VAM are also discussed as important biofertilizers. The document provides examples of various biofertilizers used in tea cultivation and provides guidance on effective dosage and application methods to maximize the benefits of biofertilizers.
Isolation of phosphate solubilizing bacteria (PSB) from soil Likhith KLIKHITHK1
A number of bacterial species provide beneficial effects to a plant and these are mostly present in rhizosphere and hence called rhizobacteria. This group of bacteria has been termed plant growth promoting rhizobacteria. Phosphorus is an essential element for plant development and growth making up about 0.2 % of plant dry weight. Plants acquire P from soil solution as phosphate anions. However, phosphate anions are extremely reactive and may be immobilized through precipitation with cations such as Ca 2+ , Mg 2+ , Fe 3+ and Al 3+. In these forms, P is highly insoluble and unavailable to plants. Different bacterial species has ability to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, di calcium phosphate, hydroxyapatite, and rock phosphate to soluble form, Hence theses bacteria's are referred to as phosphate solubilizing bacteria.
Penicillin is one of the most commonly used antibiotics globally, as it has a wide range of clinical indications. Penicillin is effective against many different types of infections involving gram-positive cocci, gram-positive rods (e.g., Listeria), most anaerobes, and gram-negative cocci (e.g., Neisseria). Importantly, certain bacterial species have obtained penicillin resistance, including enterococci. Enterococci infections now receive treatment with a combination of penicillin and streptomycin or gentamicin. Certain gram-negative rods are also resistant to penicillin due to penicillin’s poor ability to penetrate the porin channel. However, later generations of broad-spectrum penicillins are effective against gram-negative rods. Second-generation penicillins (ampicillin and amoxicillin) can also penetrate the porin channel, making these drugs effective against Proteus mirabilis, Shigella, H. influenzae, Salmonella, and E. coli. Third-generation penicillins such as carbenicillin and ticarcillin are also able to penetrate gram-negative bacterial porin channels. Fourth-generation penicillins such as piperacillin are effective against the same bacterial strains as third-generation penicillins as well as Klebsiella, enterococci, Pseudomonas aeruginosa, and Bacteroides fragilis.
ABSTRACT- The isolation of phosphate solubilizing bacterial strains exhibiting high ability to solubilize soil
phosphorus is a matter of great interest with high applicability. The use of phosphate solubilizing bacteria as inoculants
simultaneously increases phosphate uptake by the plant and increase crop yield. Strains from the genera Pseudomonas,
Bacillus and Rhizobium species are among the most powerful phosphate solubilizers. In this present study different
cultivated soil samples were investigated for the isolation of phosphate solubilizing bacteria by Pikovskayas agar media.
We were found 2 bacterial strains SS1 and SS2 as a phosphate solubilizing bacteria. Both two bacteria are characterized
by morphological and biochemical tests. The strain SS2 was confirmed as a Bacillus megaterium. Then Bacillus
megaterium is used for the production of longer sustainable phosphate solbulizing biofertilizer. After an interval of 180
days it has 5x1013 CFU count.
Key-words- Phosphate solubilization; Soil bacteria; Plant-growth-promoting bacteria; Rhizobacteria; Phosphates;
Biofertilizer
Production and Application of Phosphate Solubilizing Bacteria as Biofertilize...Agriculture Journal IJOEAR
Abstract— Soil microorganism plays an important role in regulating the levels of carbon, nitrogen, phosphorus and sulphur at the rhizosphere. Solubilization of macronutrient is an important aspect in plant growth and development research. Phosphorus is one of the vital nutrient required for optimum growth of plant. Phosphate Solubilizing Bacteria (PSB) plays an important role in increasing the phosphate uptake by the plants. Pikovskaya medium containing TCP or tricalcium phosphate helps in isolation of PSB from soil sample. Isolated strains were tested and screened by Halo zone formation and pH test. The selected strains showing marked decrease in pH and clear zone formation was selected for field trial. The objective of this study was to ascertain that PSBs isolated from the soil sample can be used as biofertilizer. Application of PSBs with a carrier mixed with maize seeds in the field of study and compared against a control field and against commercially available fertilizer showed that PSBs can improve the quality of soil and in turn improved the growth and development of the plants.
Isolation of phosphate solubilizing bacteria (PSB) from soil Likhith KLIKHITHK1
A number of bacterial species provide beneficial effects to a plant and these are mostly present in rhizosphere and hence called rhizobacteria. This group of bacteria has been termed plant growth promoting rhizobacteria. Phosphorus is an essential element for plant development and growth making up about 0.2 % of plant dry weight. Plants acquire P from soil solution as phosphate anions. However, phosphate anions are extremely reactive and may be immobilized through precipitation with cations such as Ca 2+ , Mg 2+ , Fe 3+ and Al 3+. In these forms, P is highly insoluble and unavailable to plants. Different bacterial species has ability to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, di calcium phosphate, hydroxyapatite, and rock phosphate to soluble form, Hence theses bacteria's are referred to as phosphate solubilizing bacteria.
Penicillin is one of the most commonly used antibiotics globally, as it has a wide range of clinical indications. Penicillin is effective against many different types of infections involving gram-positive cocci, gram-positive rods (e.g., Listeria), most anaerobes, and gram-negative cocci (e.g., Neisseria). Importantly, certain bacterial species have obtained penicillin resistance, including enterococci. Enterococci infections now receive treatment with a combination of penicillin and streptomycin or gentamicin. Certain gram-negative rods are also resistant to penicillin due to penicillin’s poor ability to penetrate the porin channel. However, later generations of broad-spectrum penicillins are effective against gram-negative rods. Second-generation penicillins (ampicillin and amoxicillin) can also penetrate the porin channel, making these drugs effective against Proteus mirabilis, Shigella, H. influenzae, Salmonella, and E. coli. Third-generation penicillins such as carbenicillin and ticarcillin are also able to penetrate gram-negative bacterial porin channels. Fourth-generation penicillins such as piperacillin are effective against the same bacterial strains as third-generation penicillins as well as Klebsiella, enterococci, Pseudomonas aeruginosa, and Bacteroides fragilis.
ABSTRACT- The isolation of phosphate solubilizing bacterial strains exhibiting high ability to solubilize soil
phosphorus is a matter of great interest with high applicability. The use of phosphate solubilizing bacteria as inoculants
simultaneously increases phosphate uptake by the plant and increase crop yield. Strains from the genera Pseudomonas,
Bacillus and Rhizobium species are among the most powerful phosphate solubilizers. In this present study different
cultivated soil samples were investigated for the isolation of phosphate solubilizing bacteria by Pikovskayas agar media.
We were found 2 bacterial strains SS1 and SS2 as a phosphate solubilizing bacteria. Both two bacteria are characterized
by morphological and biochemical tests. The strain SS2 was confirmed as a Bacillus megaterium. Then Bacillus
megaterium is used for the production of longer sustainable phosphate solbulizing biofertilizer. After an interval of 180
days it has 5x1013 CFU count.
Key-words- Phosphate solubilization; Soil bacteria; Plant-growth-promoting bacteria; Rhizobacteria; Phosphates;
Biofertilizer
Production and Application of Phosphate Solubilizing Bacteria as Biofertilize...Agriculture Journal IJOEAR
Abstract— Soil microorganism plays an important role in regulating the levels of carbon, nitrogen, phosphorus and sulphur at the rhizosphere. Solubilization of macronutrient is an important aspect in plant growth and development research. Phosphorus is one of the vital nutrient required for optimum growth of plant. Phosphate Solubilizing Bacteria (PSB) plays an important role in increasing the phosphate uptake by the plants. Pikovskaya medium containing TCP or tricalcium phosphate helps in isolation of PSB from soil sample. Isolated strains were tested and screened by Halo zone formation and pH test. The selected strains showing marked decrease in pH and clear zone formation was selected for field trial. The objective of this study was to ascertain that PSBs isolated from the soil sample can be used as biofertilizer. Application of PSBs with a carrier mixed with maize seeds in the field of study and compared against a control field and against commercially available fertilizer showed that PSBs can improve the quality of soil and in turn improved the growth and development of the plants.
In aquaculture, environmental impacts such as deteriorated water quality and poor pond bottoms are becoming challenging and omnipresent problems. This article highlights measures which can be taken to improve the quality of water and soil in aquaculture ponds, and therefore the immediate environment of fish and shrimp. Better rearing conditions will improve the overall performance of your fish and shrimp.
Use of biofertilizers is one of the important components of integrated nutrient management, as they are cost effective and renewable source of plant nutrients to supplement the chemical fertilizers for sustainable agriculture This ppt is very essential & useful for vegetable crop production, because present time the farmers was used fertilizers is more compared to the recommended dose of fertilizer. so i can suggested the farmers use of bio fertilizer because they have farmers ecofriendly.
The most commonly found biofertilizers in the market today are the potassium mobilizing biofertilizer, zinc solubilizing biofertilizer, Azotobacter, and many more.
Biopesticide refers introduction of any living organism such as microorganism including bacteria , fungi , nematodes viruses, protozoa and parasitoids and predators that controls pests by biological non-toxic means e.g. Trichoderma sp., Bacillus thuringiensis, Beauveria etc.
Economic importance of bacteria or application of bacteriaAbhay jha
Economic importance of bacteria their uses and application in everyday lif. Harms by bacteria different disease and profit of bacteria to be available in our environment and fora and funa. Different types of disease which are caused by bacteria to animals,humans and plants.
Roles in agriculture , Decay and Decomposition,
Role in medicine ,
Role in biofuels.
Biotechnology
Genetic engineering
Biofertilizers have enough strength to improve the productivity of crop plants as well as soil health. In this slide various biofertilizers, their role, use, application methods are briefly described.
CHEMICALS AND DRUGS USED IN SHRIMP AQUACULTUREICAR-CIFE
In aquaculture, as in all food production sectors, one of the external inputs required for successful fish production is chemical.
Chemicals are indeed an essential ingredient to successful aquaculture, which has been used in various forms for centuries (Subasinghe et al., 1996).
There is no doubt that some products are very useful, however, may not perform as claimed and farmers must evaluate the cost and benefits of treatment before investing in any products.
Information on how the product works should be studied. Several products have been sold without any explanation of their action.
Other alternatives could be used as bioremediation and use of probiotics, immunostimulants, vaccination, and alternative therapies.
Biofertilizers is one such component of organic farming that keep the soil environment rich in all kinds of micro- and macro-nutrients via nitrogen fixation, phosphate and potassium solubilisation or mineralization, release of plant growth regulating substances, production of antibiotics and biodegradation of organic matter in the soil. When biofertilizers are applied as seed or soil inoculants, they multiply and participate in nutrient cycling and benefit crop productivity. In general, 60% to 90% of the total applied fertilizer is lost and the remaining 10% to 40% is taken up by plants. Biofertilizers improve soil fertility by fixing the atmospheric nitrogen and solubilising insoluble phosphates and produce plant growth-promoting substances in the soil.
In aquaculture, environmental impacts such as deteriorated water quality and poor pond bottoms are becoming challenging and omnipresent problems. This article highlights measures which can be taken to improve the quality of water and soil in aquaculture ponds, and therefore the immediate environment of fish and shrimp. Better rearing conditions will improve the overall performance of your fish and shrimp.
Use of biofertilizers is one of the important components of integrated nutrient management, as they are cost effective and renewable source of plant nutrients to supplement the chemical fertilizers for sustainable agriculture This ppt is very essential & useful for vegetable crop production, because present time the farmers was used fertilizers is more compared to the recommended dose of fertilizer. so i can suggested the farmers use of bio fertilizer because they have farmers ecofriendly.
The most commonly found biofertilizers in the market today are the potassium mobilizing biofertilizer, zinc solubilizing biofertilizer, Azotobacter, and many more.
Biopesticide refers introduction of any living organism such as microorganism including bacteria , fungi , nematodes viruses, protozoa and parasitoids and predators that controls pests by biological non-toxic means e.g. Trichoderma sp., Bacillus thuringiensis, Beauveria etc.
Economic importance of bacteria or application of bacteriaAbhay jha
Economic importance of bacteria their uses and application in everyday lif. Harms by bacteria different disease and profit of bacteria to be available in our environment and fora and funa. Different types of disease which are caused by bacteria to animals,humans and plants.
Roles in agriculture , Decay and Decomposition,
Role in medicine ,
Role in biofuels.
Biotechnology
Genetic engineering
Biofertilizers have enough strength to improve the productivity of crop plants as well as soil health. In this slide various biofertilizers, their role, use, application methods are briefly described.
CHEMICALS AND DRUGS USED IN SHRIMP AQUACULTUREICAR-CIFE
In aquaculture, as in all food production sectors, one of the external inputs required for successful fish production is chemical.
Chemicals are indeed an essential ingredient to successful aquaculture, which has been used in various forms for centuries (Subasinghe et al., 1996).
There is no doubt that some products are very useful, however, may not perform as claimed and farmers must evaluate the cost and benefits of treatment before investing in any products.
Information on how the product works should be studied. Several products have been sold without any explanation of their action.
Other alternatives could be used as bioremediation and use of probiotics, immunostimulants, vaccination, and alternative therapies.
Biofertilizers is one such component of organic farming that keep the soil environment rich in all kinds of micro- and macro-nutrients via nitrogen fixation, phosphate and potassium solubilisation or mineralization, release of plant growth regulating substances, production of antibiotics and biodegradation of organic matter in the soil. When biofertilizers are applied as seed or soil inoculants, they multiply and participate in nutrient cycling and benefit crop productivity. In general, 60% to 90% of the total applied fertilizer is lost and the remaining 10% to 40% is taken up by plants. Biofertilizers improve soil fertility by fixing the atmospheric nitrogen and solubilising insoluble phosphates and produce plant growth-promoting substances in the soil.
This presentation will cover mainly Bio-Fertilizers, This presentation is given by Miss Khunsha Fatima, Bio-Fertilizers, thier classification and importance discussed in detail.
Biofertilizers are living microbes that enhance plant nutrition by either by mobilizing or increasing nutrient availability in soils. Various microbial taxa including beneficial bacteria and fungi are currently used as biofertilizers, as they successfully colonize the rhizosphere, rhizoplane or root interior.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
This slide is special for master students (MIBS & MIFB) in UUM. Also useful for readers who are interested in the topic of contemporary Islamic banking.
Executive Directors Chat Leveraging AI for Diversity, Equity, and InclusionTechSoup
Let’s explore the intersection of technology and equity in the final session of our DEI series. Discover how AI tools, like ChatGPT, can be used to support and enhance your nonprofit's DEI initiatives. Participants will gain insights into practical AI applications and get tips for leveraging technology to advance their DEI goals.
A review of the growth of the Israel Genealogy Research Association Database Collection for the last 12 months. Our collection is now passed the 3 million mark and still growing. See which archives have contributed the most. See the different types of records we have, and which years have had records added. You can also see what we have for the future.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
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Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
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Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
2. ⮚ Biofertilizer technology is not a new concept.
⮚Beneficial microorganisms like bacteria, fungi and actinomycetes
considered as bio fertilizers.
⮚Environment ecofriendly non-bulky, low cost renewable source of
plant nutrients.
⮚Plays an important role in improving nutrient supplies and their
crop availability.
⮚To use live formulations of such beneficial microorganisms which
on application to seed, root or soil mobilize the availability of
nutrients by their biological activity.
INTRODUCTION
3. Bio inoculants
⮚ They are freely available in topsoil and usually associate with
plants having symbiotic relationship.
⮚ These organisms receive nutrients from the plant tissue and in
turn supply the required nitrogen and phosphorus to plants.
⮚ Bio-inoculants like Azotobacter, Azospirillum, PSB and VAM
could regarded as broad spectrum biofertilizer.
6. NITROGEN FIXING BIOFERTILIZER
(BACTERIA)
i) eg: Acetobacter
• Nitrogen fixation is a process
by which nitrogen in the
atmosphere is converted into
ammonium or related nitrogenous
compounds
• Acetobacter is a free living
diazotropic bacteria found in
neutral to alkaline soils in aquatic
environment.
•Metabolic nitrogen fixation by
conversion to ammonium
7. NITROGEN FIXING BIOFERTILIZER (BACTERIA)
ii) eg:
Rhizobium(legume)
I. Rhizobium is a soil
bacteria present in the roots
nodules of leguminous
plants.
II. leads to the formation of
lumps or nodules where the
nitrogen fixation takes place.
III. They convert nitrogen gas in
the atmosphere into
ammonium form a usable by
the plants.
8. NITROGEN FIXING BIOFERTILIZER (BACTERIA)
iii) eg:
Azospirillum
i. Gram-positive bacterium
ii. Non-fermentative
iii. This bacteria can promote plant
growth.
iv. Nitrogen-fixing bacterium when
applied to the soil undergoes
multiplication in billions and fixes
atmospheric nitrogen in the soil.
9. BLUE GREEN ALGAE
⮚ Another group free living nitrogen
fixer called Cyanobacteria.
⮚ Commonly called as Blue green Algae .
⮚ More than 100 species of blue green
algae fix nitrogen.
⮚ Nitrogen fixation takes place in
specialised cells called heterocysts.
⮚ BGA can seen mostly in paddy fields.
Orysa sativa
11. PHOSPHATE SOLUBILISING BACTERIA
i. Major element required for plant
growth
ii. Rock phosphate one of the basic
raw material
iii. Monocalcium phosphate
converted into dicalcium
phosphate which is slowly
available to plants.
iv. Bacillus and Pseudomonas ,
Aspergillus and Penicillium make
insoluble phosphorus to the
plants.
Pseudomonas
putida
PSB
12. MYCORRHIZAE (FUNGI)
• Mycorrhizae is a mustalistic
association between fungal mycelia
and plant root .
•Play a key role in nutrient cycling in
ecosystem.
•It is a potential biofertilizer mobilizes
P, Fe, Zn and B other trace elements.
•Improvement in rooting and nutrient
uptake.
13. VESICULAR ARBUSCULAR MYCORRHIZAE
(VAM)
⮚ It is an endotrophic (live inside) mycorrhizae
formed by aseptated phycomycetous fungi
⮚ Vam help in nutrient transfer mainly of
phosphorus , zinc and sulphur.
⮚ They also mobilize different nutrients like Cu,
K, Al, Mn, Fe and Mg from the soil to the
roots.
⮚ They posses vesicules (sac like structure) for
storage of nutrients and arbuscular for
funneling them into the root system.
⮚ Plays a great role in inducing plant growth.
VAM FUNGI
14. PLANT GROWTH PROMOTING RHIZOBACTERIA(PGPR)
i. root colonizing rhizosphere bacteria.
ii. Rhizosphere - high microbial
activity.
iii. enhances plant growth and yield
iv. PGPR as biofertilizers are well
recognized as efficient soil microbes
for sustainable agriculture
Rhizobacteria
15. ⮚ Renewable source of nutrients
⮚ Sustain soil health
⮚ Supplement chemical fertilizers.
⮚ Replace 25-30% chemical fertilizers
⮚ Increase the grain yields by 10-40%.
⮚ Decompose plant residues, and stabilize C:N ratio of soil
⮚ Improve texture, structure and water holding capacity of soil
⮚ No adverse effect on plant growth and soil fertility.
⮚ Stimulates plant growth by secreting growth hormones.
⮚ Solubilize and mobilize nutrients
⮚ Eco-friendly, non-pollutants and cost effective method
ADVANTAGES OF BIOFERTILIZER
16. Name of the organism
NCBI Accession
Number
Role
Azospirillum brasilense (AB) &
A. lipoferum (CT8)
- Nitrogen fixers
Pseudomonas putida (PB) &
P. fluorescens (TRB)
KM527837
Phosphate solubilizing
bacteria (PSB)
Pseudomonas putida (KSB1) &
Burkholderia cepacia (KSB2)
-
Potassium solubilizing
bacteria (KSB)
Trichoderma atroviride (TA) - Biocontrol agent
Arbuscular mycorrhizae (AMF) - Phosphate mobilize
Bacillus amyloliquefaciens (WPI04) KM853034
Biodegradation
bacteria/Biopesticides
Bacillus licheniformis (AWRH40-B) KM527838
Bacillus thuringiensis (BT) KP004438
Pseudomonas putida -
Bacillus sp. (UP9Y1) HF934967
Potassium solubilizing
bacteria (KSB3)
VAM fungi
Mineral solubilizer
/Phosphate mobilize
LIST OF BIOFERTILIZERS IN TEA
Commercial bioformulation: Biodyne N and Biodyne P
20. DOSAGE AND METHOD OF
BIOFERTILIZER APPLICATION
⮚ Biofertilizers @ 25 kg/ha each of Azospirillum and Phospobacteria
formulations
⮚ It is advisable to apply the formulation after mixing it with well decomposed
farmyard manure, compost or forest soil. It is desirable to apply the mixture
by placement, near the root zone
⮚ Two split application, one premonsoon and another post monsoon, is more
efficient than one time application. While applying, the soil should not be
too wet or too dry
⮚ April/May and September/October are ideal months for the application of
biofertilizers
21. HOW TO USE BIOFERTILIZERS
⮚ The soil should be moist
⮚ Apply exactly half the amount of recommended fertilizer to the area
biofertilizers is going to be used.
⮚ Apply biofertilizers within 7-10 days of chemical fertilizer application.
⮚ For best results apply Biofertilizers soon after application of chemical
fertilizer.
22. IMPORTANT
⮚ Store in a dry cool place. Keep away from direct sunlight
⮚ Refrain from applying any soil applied weedicides one week before
and after application of biofertilizers.
⮚ Soil testing for the enumeration of introduced organisms is essential
to make sure of their survival in tea soil as per microbial specification