Abstract
We present an indoor forensic case that occurred in spring 2013 in Cosenza (southern Italy). The entomological evidence collected at the scene consisted of Calliphoridae (Calliphora vicina, Lucilia sericata), Sarcophagidae (Sarcophaga crassipalpis), Fanniidae (Fannia scalaris) and Muscidae (Hydrotaea ignava). The minimum Post Mortem Interval (mPMI) was calculated by relating the entomological evidence to data available for Diptera species in the area and to our knowledge of the development of flies used as forensic indicators in Calabria. We report S. crassipalpis as a corpse invader for the first time in Italy.
Keywords: Forensic case; Flies; S. crassipalpis; mPMI; Southern Italy
Molecular Study of Cutaneous Leishmaniasis Human Reservoirs and Infections in...CrimsonpublishersCJMI
Molecular Study of Cutaneous Leishmaniasis Human Reservoirs and Infections in Bastak by Houshang Jamali in Cohesive Journal of Microbiology & Infectious Disease
Forensic Entomology is that special associated distinctive discipline of Forensic Science that deals with the assorted aspects of an insect’s or a maggot’s life cycle so as to assess the time since death.
Molecular Study of Cutaneous Leishmaniasis Human Reservoirs and Infections in...CrimsonpublishersCJMI
Molecular Study of Cutaneous Leishmaniasis Human Reservoirs and Infections in Bastak by Houshang Jamali in Cohesive Journal of Microbiology & Infectious Disease
Forensic Entomology is that special associated distinctive discipline of Forensic Science that deals with the assorted aspects of an insect’s or a maggot’s life cycle so as to assess the time since death.
Succession of Arthropods on White Rat Carcasses in Ile Ife, Southwestern Nigeriaijtsrd
The forensic information provided by decomposition of small carcasses often goes unnoticed, even in advanced economies, due to frequent neglect. This paper reports the succession pattern of arthropod species that associated with carcasses of white rat, Rattus norvegicus Berkenhout Rodentia Muridae , in Ile Ife, southwestern Nigeria. Four bushy sites were chosen for the study and nine rat carcasses were placed at each site once a season for two seasons. The carcasses were monitored daily until the process of decay was over. The visiting and colonizing invertebrates were collected daily and identified. Immatures were also collected and reared in the laboratory till adult emergence for easy identification. The carcasses went through five stages of decay and the arthropods arrived in the order Diptera early fresh stage , Hymenoptera late fresh stage , Coleoptera and Dermaptera active decay stage , and Araneae and Oribatida advanced decay stage . Dipteran flies were the first arthropods to interact with the remains but ants were the only arthropods that associated with all the five stages of decay. A total of 9828 arthropods 4415 adults and 5413 immatures belonging to six orders in two classes of the phylum were collected in the study. The proportion of faunal abundance was Diptera 75.10 , Hymenoptera 22.90 , Coleoptera 1.80 , Dermaptera 0.10 , Oribatida 0.08 and Araneae 0.02 . Rate of decay was faster and faunal population was higher on carcasses during the dry season compared to the wet. Faunal population was also higher on carcasses placed in close proximity to the Zoological garden. The implications of these results on accuracy of estimated postmortem interval PMI and applicability in law were discussed. Aminat Adeola Adesina | Olalekan Joseph Soyelu "Succession of Arthropods on White Rat Carcasses in Ile-Ife, Southwestern Nigeria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-6 , October 2020, URL: https://www.ijtsrd.com/papers/ijtsrd35737.pdf Paper Url: https://www.ijtsrd.com/biological-science/zoology/35737/succession-of-arthropods-on-white-rat-carcasses-in-ileife-southwestern-nigeria/aminat-adeola-adesina
palynotaxa and parasitic loads of nigerian currency potential sources of mic...INFOGAIN PUBLICATION
Currency notes are handled by a large number of people under a variety of personal and environmental conditions. A total of ninety six samples of one hundred naira denomination of Nigerian notes were procured from seven Local Government Areas (LGA) of Ebonyi State, Nigeria. The aim of the study was to determine the palynotaxa and parasitic load prevalent on currency notes. The leachates of currency notes were obtained and subjected to acetolysis and examined microscopically. Twenty six fungal spores type were recorded and were highly dominated by spores of Libertelli spp., Botrytis spp. and Spadicoides spp. Pollen achieved 54 % of the total bio-particles, whereas fungal spores and parasitic worms achieved 35.2 % and 10.60 %, respectively. The presence and relative abundance of these palynotaxa and parasites in currency notes affirms their propensity to spread vectors of diseases.
'INCIDENCE OF HYDATIDOSIS IN SLAUGHTERED CATTLE, BUFFALOES AND GOATS’, 'MOLEC...Hrishikesh Khakse
1) Ingole R. S., Khakse H. D., et.al. ‘INCIDENCE OF HYDATIDOSIS IN SLAUGHTERED CATTLE, BUFFALOES AND GOATS’ Published in Abstract Compendium of 31st Annual Conference of Indian Association of Veterinary Pathologist, National Symposium on “Impact of Climate Change on Pathology of Disease of Animals, Poultry and Fish” 13th to 15th Nov. 2014, organized by Anand Agriculture University, Anand, Sr. No. 41, Page 86. 2) Ingole R. S., Khakse H. D., et.al. ‘MOLECULAR CHARACTERIZATION OF ECHINOCOCCUS GRANULOSUS IN ANIMALS BY SEQUENCING OF COX-1 GENE’ Published in Abstract Compendium of 31st Annual Conference of Indian Association of Veterinary Pathologist, National Symposium on “Impact of Climate Change on Pathology of Disease of Animals, Poultry and Fish” 13th to 15th Nov. 2014, organized by Anand Agriculture University, Anand, Sr. No. 28, Page 86. 3) Ingole R. S., Khakse H. D., et.al. ‘PREVALENCE OF ECHINOCOCCUS GRANULOSUS IN DOGS BY POLYMERASE CHAIN REACTION’ Published in Abstract Compendium of 31st Annual Conference of Indian Association of Veterinary Pathologist, National Symposium on “Impact of Climate Change on Pathology of Disease of Animals, Poultry and Fish” 13th to 15th Nov. 2014, organized by Anand Agriculture University, Anand, Sr. No. 29, Page 86.
— Herpesviruses that infect fishes belong to the Herpesvirales order and Alloherpesvirus family. In these species, the different types of herpesvirus can cause tumors, adenocarcinoma and skin lesions. This study aims detect to presence of herpesvirus in fishes from commercial, recreation or experimental creations of the States of São Paulo and Minas Gerais, Brazil. Organ fragments and lesions of 53 fish species coming of mortality cases were forwarded at Biological Institute for examination by transmission electron microscopy by research of etiological agent. By transmission electron microscopy through negative staining technique, were observed herpes virus-like particles in 46 fishes and through embedding resin technique, in ultrathin sections were visualized herpes virus immature particles, measuring 90-110nm in diameter, located in the nuclei and complete particles measuring 160nm. In the histopathology technique, lesions associated with the virus as corpuscles inclusion, papillomas, and dermal lesions and in the gills were observed in 27 fishes. The evaluated techniques of TEM and the histopathology were effective for the rapid detection of herpesvirus in the examined samples.
"Cell", article entitled ""Heroes of CRISPR"Lynsey Wiggins
New gene-editing technology discovered recently that could change the whole world. Amazing implications! Researchers are fighting over patent rights and in the mean time tons of money is being made.
This master's seminar presentation speaks about the role of bacteriophage in the management of different plant diseases.
It deals with the history and discovery of bacteriophages up to current research studies and usage.
Abrachium, a new genus in the Clathraceae, and Itajahya reassessedRhudson Cruz
Molecular and morphological analyses have elucidated phylogenetic relationships of two remarkable species in the Phallales: Aseroe floriformis and Phallus roseus. Genes from ATPase subunit 6 (atp6), the nuclear large subunit ribossomal DNA (nuc-LSU), and the second largest RNA polymerase II subunit (RPB2) underwent Bayesian and parsimony molecular analyses. Molecular datasets combined with morphological characters, support a new genus (Abrachium for Aseroe floriformis), reassessment Itajahya, and emendation of Clathraceae.
Forensic Entomology is the study of insects found at the crime scene.
For complete explanation of videos, click on the link- https://youtu.be/RXs9BKFkUXk.
Annex Publishers, as an Open Access publication model allows the dissemination of research articles to the worldwide community. We offer you the advantage of interaction with the most effective minds from the scientific community. All articles printed under open access will be accessed by anyone.
www.annexpublishers.com
Abstract
Here in a systematic, accurate and reliable method, Head-Space Gas Chromatography-Mass Spectrometry (HS-GC/MS) was developed to determine blood carboxyhemoglobin (COHb%), in order to investigate deaths related to CO exposure especially involving blood and hepatic tissues. Using a column packed with molecular sieve, COHb levels were quantified down to 0.2% in small blood samples quickly and showed good reproducibility with RSD of the COHb < 1%. COHb% in hepatic samples stored at different temperatures (-20 °C for 1- 2 years, 0 °C, and 18 °C for two months) can be determined even when the samples are decomposed. The 3-min procedure requires only 0.25 mL of blood sample or 1.0 g of hepatic tissue each time. The technique has a clear advantage over other methods such as UV spectrophotometry.
Keywords: Carbon monoxide poisoning; COHb; Decomposed hepatic tissues; Headspace gas chromatography/mass spectrometry (HS-GC /MS)
Succession of Arthropods on White Rat Carcasses in Ile Ife, Southwestern Nigeriaijtsrd
The forensic information provided by decomposition of small carcasses often goes unnoticed, even in advanced economies, due to frequent neglect. This paper reports the succession pattern of arthropod species that associated with carcasses of white rat, Rattus norvegicus Berkenhout Rodentia Muridae , in Ile Ife, southwestern Nigeria. Four bushy sites were chosen for the study and nine rat carcasses were placed at each site once a season for two seasons. The carcasses were monitored daily until the process of decay was over. The visiting and colonizing invertebrates were collected daily and identified. Immatures were also collected and reared in the laboratory till adult emergence for easy identification. The carcasses went through five stages of decay and the arthropods arrived in the order Diptera early fresh stage , Hymenoptera late fresh stage , Coleoptera and Dermaptera active decay stage , and Araneae and Oribatida advanced decay stage . Dipteran flies were the first arthropods to interact with the remains but ants were the only arthropods that associated with all the five stages of decay. A total of 9828 arthropods 4415 adults and 5413 immatures belonging to six orders in two classes of the phylum were collected in the study. The proportion of faunal abundance was Diptera 75.10 , Hymenoptera 22.90 , Coleoptera 1.80 , Dermaptera 0.10 , Oribatida 0.08 and Araneae 0.02 . Rate of decay was faster and faunal population was higher on carcasses during the dry season compared to the wet. Faunal population was also higher on carcasses placed in close proximity to the Zoological garden. The implications of these results on accuracy of estimated postmortem interval PMI and applicability in law were discussed. Aminat Adeola Adesina | Olalekan Joseph Soyelu "Succession of Arthropods on White Rat Carcasses in Ile-Ife, Southwestern Nigeria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-6 , October 2020, URL: https://www.ijtsrd.com/papers/ijtsrd35737.pdf Paper Url: https://www.ijtsrd.com/biological-science/zoology/35737/succession-of-arthropods-on-white-rat-carcasses-in-ileife-southwestern-nigeria/aminat-adeola-adesina
palynotaxa and parasitic loads of nigerian currency potential sources of mic...INFOGAIN PUBLICATION
Currency notes are handled by a large number of people under a variety of personal and environmental conditions. A total of ninety six samples of one hundred naira denomination of Nigerian notes were procured from seven Local Government Areas (LGA) of Ebonyi State, Nigeria. The aim of the study was to determine the palynotaxa and parasitic load prevalent on currency notes. The leachates of currency notes were obtained and subjected to acetolysis and examined microscopically. Twenty six fungal spores type were recorded and were highly dominated by spores of Libertelli spp., Botrytis spp. and Spadicoides spp. Pollen achieved 54 % of the total bio-particles, whereas fungal spores and parasitic worms achieved 35.2 % and 10.60 %, respectively. The presence and relative abundance of these palynotaxa and parasites in currency notes affirms their propensity to spread vectors of diseases.
'INCIDENCE OF HYDATIDOSIS IN SLAUGHTERED CATTLE, BUFFALOES AND GOATS’, 'MOLEC...Hrishikesh Khakse
1) Ingole R. S., Khakse H. D., et.al. ‘INCIDENCE OF HYDATIDOSIS IN SLAUGHTERED CATTLE, BUFFALOES AND GOATS’ Published in Abstract Compendium of 31st Annual Conference of Indian Association of Veterinary Pathologist, National Symposium on “Impact of Climate Change on Pathology of Disease of Animals, Poultry and Fish” 13th to 15th Nov. 2014, organized by Anand Agriculture University, Anand, Sr. No. 41, Page 86. 2) Ingole R. S., Khakse H. D., et.al. ‘MOLECULAR CHARACTERIZATION OF ECHINOCOCCUS GRANULOSUS IN ANIMALS BY SEQUENCING OF COX-1 GENE’ Published in Abstract Compendium of 31st Annual Conference of Indian Association of Veterinary Pathologist, National Symposium on “Impact of Climate Change on Pathology of Disease of Animals, Poultry and Fish” 13th to 15th Nov. 2014, organized by Anand Agriculture University, Anand, Sr. No. 28, Page 86. 3) Ingole R. S., Khakse H. D., et.al. ‘PREVALENCE OF ECHINOCOCCUS GRANULOSUS IN DOGS BY POLYMERASE CHAIN REACTION’ Published in Abstract Compendium of 31st Annual Conference of Indian Association of Veterinary Pathologist, National Symposium on “Impact of Climate Change on Pathology of Disease of Animals, Poultry and Fish” 13th to 15th Nov. 2014, organized by Anand Agriculture University, Anand, Sr. No. 29, Page 86.
— Herpesviruses that infect fishes belong to the Herpesvirales order and Alloherpesvirus family. In these species, the different types of herpesvirus can cause tumors, adenocarcinoma and skin lesions. This study aims detect to presence of herpesvirus in fishes from commercial, recreation or experimental creations of the States of São Paulo and Minas Gerais, Brazil. Organ fragments and lesions of 53 fish species coming of mortality cases were forwarded at Biological Institute for examination by transmission electron microscopy by research of etiological agent. By transmission electron microscopy through negative staining technique, were observed herpes virus-like particles in 46 fishes and through embedding resin technique, in ultrathin sections were visualized herpes virus immature particles, measuring 90-110nm in diameter, located in the nuclei and complete particles measuring 160nm. In the histopathology technique, lesions associated with the virus as corpuscles inclusion, papillomas, and dermal lesions and in the gills were observed in 27 fishes. The evaluated techniques of TEM and the histopathology were effective for the rapid detection of herpesvirus in the examined samples.
"Cell", article entitled ""Heroes of CRISPR"Lynsey Wiggins
New gene-editing technology discovered recently that could change the whole world. Amazing implications! Researchers are fighting over patent rights and in the mean time tons of money is being made.
This master's seminar presentation speaks about the role of bacteriophage in the management of different plant diseases.
It deals with the history and discovery of bacteriophages up to current research studies and usage.
Abrachium, a new genus in the Clathraceae, and Itajahya reassessedRhudson Cruz
Molecular and morphological analyses have elucidated phylogenetic relationships of two remarkable species in the Phallales: Aseroe floriformis and Phallus roseus. Genes from ATPase subunit 6 (atp6), the nuclear large subunit ribossomal DNA (nuc-LSU), and the second largest RNA polymerase II subunit (RPB2) underwent Bayesian and parsimony molecular analyses. Molecular datasets combined with morphological characters, support a new genus (Abrachium for Aseroe floriformis), reassessment Itajahya, and emendation of Clathraceae.
Forensic Entomology is the study of insects found at the crime scene.
For complete explanation of videos, click on the link- https://youtu.be/RXs9BKFkUXk.
Annex Publishers, as an Open Access publication model allows the dissemination of research articles to the worldwide community. We offer you the advantage of interaction with the most effective minds from the scientific community. All articles printed under open access will be accessed by anyone.
www.annexpublishers.com
Abstract
Here in a systematic, accurate and reliable method, Head-Space Gas Chromatography-Mass Spectrometry (HS-GC/MS) was developed to determine blood carboxyhemoglobin (COHb%), in order to investigate deaths related to CO exposure especially involving blood and hepatic tissues. Using a column packed with molecular sieve, COHb levels were quantified down to 0.2% in small blood samples quickly and showed good reproducibility with RSD of the COHb < 1%. COHb% in hepatic samples stored at different temperatures (-20 °C for 1- 2 years, 0 °C, and 18 °C for two months) can be determined even when the samples are decomposed. The 3-min procedure requires only 0.25 mL of blood sample or 1.0 g of hepatic tissue each time. The technique has a clear advantage over other methods such as UV spectrophotometry.
Keywords: Carbon monoxide poisoning; COHb; Decomposed hepatic tissues; Headspace gas chromatography/mass spectrometry (HS-GC /MS)
Abstract
Rocky Mountain spotted fever, Rickettsia Rickettsii (RMSF) is a reoccurring disease in Arizona and Sonora and a public health problem due to the high risk medical complications it provokes. In the region it is transmitted by the bite of the Rhipicephalus sanguineus tick, found in dogs. This tick transmits Rickettsia rickettsii bacteria. Following an incubation period of 3-14 days, it causes acute, non-specific effects early on (fever, headache and rash) that -if not diagnosed and treated in time can be serious or cause death. Death is primarily associated with two factors: (a) delay in diagnosis, and (b) doxycycline, a highly effective antibiotic treatment which is inexpensive and simple to administer is delayed. If doxycycline is not provided before the 5th day after the symptoms begin, the patient can worsen and present with dark purple spots on the body, mostly hands and soles, wrists and ankles as well as have heart, hepatitis, renal, central nervous symptoms and other multiple organ complications. RMSF should be considered a medical priority and a public health problem at the regional level with a network of underlying factors. To prevent and control RMSF in Arizona and Sonora, public health interventions will need to address medical challenges associated with a number of social, political, and environmental factors.
Abstract
A person's stature is an identifying characteristic that is often used in forensic investigation. Footprint is the one of the valuable physical evidence encountered in the crime scenes and examination of barefoot impressions is important especially in developing countries like India, Malaysia where majority of the rural population like to walk barefooted because of socio-economic and climatic reasons. Foot impressions are still found at crime scenes, since offenders often tend to remove their footwear either to avoid noise or to gain better grip in climbing walls, etc., while entering or exiting. Most of the foot/foot print studies were conducted on mixed population. The researchers have cautioned that the people from different races and regions of a country bear different morphological features depending upon their geographical distribution and primary racial characteristics and hence a single formula cannot represent all parts of that country or world. The present study aims to estimate stature from bilateral footprints collected from 320 (160 males, 160 females) consenting adult Ibans, an indigenous group residing in Sarawak state, east Malaysia. Informed consent and Human Ethical Approval were obtained. The height of the individuals and footprints were collected following the standard procedure. The data obtained were analyzed with PASW 20 computer software and derived regression formulae to estimate stature from footprint lengths of Iban ethnics. The result of the investigation revealed that all footprint lengths exhibit statistically positive significant correlation with stature (p< 0.001). Correlation coefficient (R) values are found to be higher in the pooled sample (0.861–0.887) when compared with males (0.753–0.789) and females (0.728–0.790). The regression formulae derived for the pooled sample can be used to estimate stature when the sex of the footprint remains unknown, as in real crime scenarios.
Abstract
Age estimation of unknown human cadavers is important in setting a crime investigation, such as homicide, suicide or in mass disaster because it can guide forensic investigators to the correct identity among a large number of possible matches. More and more the courts ask for medico-legal age estimations of unknown human cadavers by forensic dentistry. This research project in establishing the applicability of radiographic and morphological parameter's used in forensic dentistry in a Portuguese adult population. The main goal is to compare the chronological age with the dental age estimation by radiographic and morphological parameters. To achieve this objective, thirty-five single-rooted teeth were analysed. Radiographic analysis was made through the program of digital radiology software Kodak RVG intra-oral 2200 with exposure factors 60-70 KVp and 7mA. The morphological parameter, translucent dentin was assessed in intact and sectioned tooth. The relationship between the coefficients was calculated between the age and the ratios with the level of inclusion, p< 0.05. There was no statistically significant difference between the chronological age and the age estimation by each method. The coefficient of Pearson for the direct measurements of the translucent dentin methodology for sectioned teeth gives the better correlation with the age estimate (r=0.86).
Abstract
A new method for improvement of 1,2-indandione-treated latent fingermarks on thermal paper will be described in this paper. Treatment with conventional techniques like ninhydrin in petroleum ether or DFO solution produces a black color on the thermosensitive side of thermal paper. The new method using 5,6-dimethoxy-1,2-indandione with polyvinyl pyrrolidone (PVP) can reduce this dark staining without removing the thermosensitive layer and parts of the developed fingermarks. An advantage of this method is that the developed fluorescent fingermarks appear in sharp lines and high contrast . The developed fingermarks can be observed when excited in the 535 nm region and using an orange-red goggles. There is no background coloration in this method. In addition, some tests were performed, leading to an optimized working solution, which charges the paper with a minimum of chemicals, is cheap, and enables a large quantity of papers to be treated in a short time and without involving any pre- or post treatment.
An interesting-coexistence-of-a-classical-nodular-and-an-adenoid-ulcerous-bas...Annex Publishers
Abstract
Basal cell carcinoma is the most frequent malignant tumor of the skin, and the most common carcinoma found in some countries. The main clinical subtypes of BCC are nodular, superficial, pigmented and morpheaform. The majority of the lesions appear on the head and neck, with a particular predilection on the upper central part of the face. Here we report a 62 year-old woman with two different morphological types of BCC which developed in the same area. The tumors have developed two months apart on the right paranasal region in close proximity. With the clinical examination and histopathological examination of punch biopsy specimens, the diagnoses of the lesions were confirmed as a nodular and an adenoid BCC. The tumors were totally removed with surgical excision. Neither recurrence nor new lesion was observed on the lesional or near areas in a 24-month follow-up period. To the best of our knowledge the presence of the two different morphological types of BCC in the same anatomical location has not been reported previously.
JOURNAL OF VETERINARY SCIENCE & ANIMAL HUSBANDRYAnnex Publishers
Journal of Veterinary Science and Animal Husbandry (JVSAH) is a scientific journal which has topics associated with fundamental and aspects of veterinary science and animal husbandry. JVSAH has a special feature of publishing special issues in experimental clinical research, veterinary medicine and current research. At Annex Publishers, we tend to publish quality articles and try our best to provide the most effective analysis journal to the scientific community.
Does physical-activity-and-sport-practice-lead-to-a-healthier-lifestyle-and-e...Annex Publishers
The prevalence of childhood obesity has been increasing rapidly and there is general consensus that good nutritional practices and physical activity should be encouraged as early as possible in life. The aim of this study was to describe and to compare the current lifestyle and dietary pattern of normal weight (NW) and overweight + obese (OW+OB) male adolescents who are physically active.
Methods: This observational and retrospective study was based on clinical records analysis of male adolescents aged 11-18 years who had undergone a medical evaluation at a Medical Sport Centre (Pavia, Italy) during 2009, and had filled in a self-administered life style questionnaire.
Results: The results showed that out of 1423 clinical records 23.0% of subjects were OW, 5.4% OB and 71.6% NW. We invited all the overweight and obese subjects to participate in the study, 308 of them (75.8%) agreed. Then we randomly enrolled an equivalent number of NW participants (n=308) in the medical evaluation at the sports center with similar characteristics as for socio-economic status, physical activity and age for a whole sample of 616 subjects. We handled them a validated lifestyle questionnaire. The questionnaire analysis was used to compare OW+OB and NW participants, as far as eating habits, sedentary activities and time spent in sports. All the subjects frequently skipped breakfast, did not consume fruit and vegetables daily and had a high soft drinks intake. Inverse correlations were found between weight and physical activity (p=0.01). Sedentary activities were preferred by about 25% and 66 % of the NW and OW+OB groups respectively. The percentage of smokers was similar within the two groups (14%).
Conclusions: Adolescents eating habits are incorrect, despite BMI and sports practice. Sports practice seems contributing to lower spare time physical inactivity, but does not improve eating habits. Public health interventions should focus on the reinforcement of leisure time physical activity, besides nutrition education and behavioral education programs in order to prevent obesity in the adulthood.
Tame to-torture-psychosexual-correlations-of-sex-violence-and-tortureAnnex Publishers
Abstract
This paper discusses the psychosexual correlations and psychological consequences related to torture and behavioral acts that are fundamentally torturous. The influence of participation by learning and the relationship of acts to thought and thought to acts are further discussed to raise awareness to the effects of torture on the torturer. Theories from multiple disciplines are presented in an integrated context to examine the correlation between fundamental psychological principles associated with acts that are torturous in nature and, therefore, innately evil.
Keywords: Aggression; Crime; Criminology; Behavioral acts; Human behavior; Psychological effects; Psychology; Psychopathology; Psychosexual; Sex; Sex crimes; Sexuality; Torture; Violence
Anti inflammatory-and-anti-arthritic-efficacy-and-safety-of-purified-shilajit...Annex Publishers
Abstract
The objective of this investigation was to evaluate the efficacy and safety of purified Shilajit in moderately arthritic dogs. Ten client-owned dogs in a randomized double-blinded study received either a placebo or Shilajit (500 mg) twice daily for a period of five months. Dogs were evaluated each month for physical condition (body weight, body temperature, heart rate, and respiration rate) and pain associated with arthritis (overall pain, pain from limb manipulation, and pain after physical exertion). Serum samples collected from these dogs were examined each month for biomarkers of liver (bilirubin, ALT, and AST), kidney (BUN and creatinine) heart and muscle (creatine kinase) functions. The findings of this study revealed that dogs receiving Shilajit (Group-II) showed a significant (P< 0.05) reduction in pain from limb manipulation by day 60, and overall pain and pain after physical exertion by day 120. Maximum pain reduction, using all three criteria, was observed on day 150. Pain level remained significantly unchanged in dogs receiving the placebo. Dogs in either group showed no significant change (P>0.05) in physical parameters or serum markers, suggesting that Shilajit was well tolerated by moderately arthritic dogs. It was concluded that Shilajit significantly (P< 0.05) reduced pain in osteoarthritic dogs and markedly improved their daily life without any side effects.
Keywords: Purified Shilajit; Osteoarthritis in canine; Shilajit safety; Anti-arthritic nutraceutical
Abstract
Salmonella is a causative agent for a wide variety of pathological diseases in humans, cattle, poultry and other farm animals and hence Salmonella infections are a major cause of concern to humans, veterinary animals and to food industry. With characterization of over 2500 Salmonella serovars, the pathogen nearly infects all vertebrates but the severity of infection varies from one serovar to another depending upon their host specificity. Some Salmonella serovars are restricted to one or few hosts while others have a broad host spectrum. Thus the understanding of the mechanisms involving host preference by one serovar over another is very important. As our knowledge about host adaptability will then be instrumental in designing better vaccines. Furthermore, methods involving identification of genetic markers for host specificity will prove to be instrumental in determining virulence factors for other pathogenic bacteria that cause systemic infections.
Keywords: Host adaption; Evolution; Salmonella serovar; Horizontal gene transfer
Case report: A 49-year-old previously healthy man was admitted to the ICU after cardiac arrest following a short history with headache, blurred speech and reduced consciousness. After cardiopulmonary resuscitation perfusion rhythm was regained, but the patient didn`t regain consciousness. The arterial blood gas analysis at the ICU revealed a severe metabolic acidosis with pH at 6.86 and lactate levels of 16 mmol/L. The white blood cells count was also markedly increased (312 * 109/L), and blood smear showed immature cells indicating acute leukemia. The severe metabolic acidosis, at first thought to be due to systemic hypoperfusion, did not improve in spite of fluid and vasopressor resuscitation. A CT scan of the head performed the next day, revealed massive cerebellar haemorrhage, edema in both hemispheres and signs of anoxic brain damage. Immunophenotyping of peripheral blood was consistent with Acute Myeloid Leukemia (AML).
Background: Observational studies have shown that low folate status induces depression. Here we studied the effects of folic acid supplementation on depression scores of Japanese women with depression-susceptible gene polymorphisms: methylenetetrahydrofolate reductase (MTHFR), serotonin transporter (5-HTT), and dopamine D4 receptor (DRD4).
Design: This was a 16-wk randomized, double-blind, crossover study of a group of Japanese women. Forty-six women (mean age 20 years; range: 18-26) were included in the study. First it was determined if the subjects had one of the following depression-susceptible gene polymorphisms: MTHFR, 5-HTT, or DRD4. The subjects then consumed bread with or without folic acid (200 μg/d) during two 4-wk diet periods separated by an 8-wk washout period. Serum folate and serum total homocysteine (tHcy) concentrations were measured before and after each intervention. Depressive symptoms using the Center for Epidemiologic Studies Depression Scale (CES-D) were determined.
Results:In the folic acid period, serum folate concentration increased from 14.2 nmol/L (median, 25th-75th percentiles: 10.6-17.5) to 23.1 nmol/L (16.4-27.8 p < 0.001); tHcy was reduced from 6.6 µmol/L (5.6-7.5) to 5.7 µmol/L (4.8-6.6, p < 0.001) and CES-D score decreased from 10.0 (3.0-13.0) to 5.0 (2.0-10.0, p < 0.001). In the control period, these changes were either insignificant or reversed. CES-D score significantly decreased by supplementing with folic acid in subjects with depression-susceptible genotypes common among Japanese, namely homozygotes of the "S" allele of 5-HTT and the "4" allele of DRD4.
Abstract
Programming scripts were written for the statistical analysis of genetic data from simple cases and complex cases of undetermined paternity. The methods presented here involve algorithms constructed with R, an open-source and increasingly popular programming language used for calculations and statistics; these methods also involve conditional probability analysis, Bayes’ Theorem, and pedigree analysis. Previous computer programs for assessing probable paternity in complex cases of undetermined paternity have been written; however, only minimal or generalized formulas are described in the papers presenting these programs. Therefore, these previously published programs are difficult to understand for most forensic researchers. Here, we present the details of the calculations used to evaluate probabilities of paternity and the details of the R scripts used execute these calculations. These scripts were constructed not only for standard trio case where DNA typing of the mother, child, and the alleged father are available, but also for more complex cases where DNA typing of the alleged father is absent. In these more complex cases, the putative genotype of the alleged father is determined from the genotypes of his parents, his siblings, his wife, children known to be his biological children, or some combination of these people. This report provides concrete and orderly descriptions of the calculations and the R scripts so that each facet of this method is easily understood. Furthermore, access to these scripts will enable individual researchers to develop calculation systems of their own.
Keywords: Forensic mathematics; Paternity testing; R; DNA typing
Journal of Biometrics and Its Applications (JBIA) is peer reviewed open access journal which addresses the fundamental areas in computer science that deal with biological measurements. It covers both the theoretical and practical aspects of human identification and verification. Biometrics based authentication, an integral component of identity science, is now being utilized in several applications playing a central role in personal, national and global security. Biometric refers to the field of development of statistical and mathematical methods applicable to data analysis problems in the biological sciences.
Study on-efficiency-of-protein-extractants-employed-for-human-origin-determin...Annex Publishers
Abstract
Human origin determination is an important aspect of blood grouping analysis in forensic science laboratories. In the present study, protein extractants like gel buffer, ammonia and saline employed for origin determination were evaluated and compared qualitatively and quantitatively for their role in the extraction of proteins from dried blood stained materials of human origin at regular time intervals. Qualitative and quantitative methods employing counter immunoelectrophoresis (CIE) and rocket immunoelectrophoresis (RIE) respectively were used to study the protein extraction efficiency of extractants. Ammonia, compared to gel buffer and saline extracted the proteins effectively. Maximum extraction of proteins was observed in 2-3 hours of sample. CIE demonstrated sharp precipitin bands with all samples of ammonia extractant compared to the samples of counterparts. RIE also revealed greater concentration of proteins in the ammonia extract compared to extracts of gel buffer and saline. These results provide evidence that ammonia serves as a better protein extractant for rapid determination of human blood origin.
Keywords: Forensic science; Forensic serology; Blood origin; Electrophoresis; Protein extractants; Immunoprecipitation
Abstract
Juvenile ossifying fibroma is an uncommon clinical entity, its aggressive local behaviour and high recurrence rate mean that it is important to make an early diagnosis, apply the appropriate treatment and, especially, follow the patient up over the long term. In the current article we report a case of juvenile ossifying fibroma-WHO type in 12yr old patient which was clinical and histopathologically challenging as it was asymptomatic and at an unusual location.
Diversity of Butterflies (Rhopalocera) inBulusukan (San Idelfonso, Bulacan, P...INFOGAIN PUBLICATION
There are 1,615 species and sub species of butterflies in the entire Philippines (C.R Baltazar, 1991), LUBG San Fernando La Union has 104 species recorded they belong to 6 families and 66 genera(Nacua et al2015).In Manila,Nacua (2016) 22 species of butterflies belong to 6 families 17 genera were also recorded. Thisstudyseeks to determine the species composition, conservation status, richness and abundance of butterflies in the town of Bulusukan, a community in San Ildefonso, Bulacan province (Luzon Island, Philippines). The opportunistic transect sampling method was used to collect butterflies. Collection was done at daytime on August 6, 2016 from 8 am up to 5 pm in areas with GPS of 15°04'26.0652"northand 121°02'39.9588"east near the vicinity of Bulusukan Cave.Species richness and butterfly diversity in all areas sampled was calculated. A dendogram showing 75% species abundance was accounted and comes mostly from the secondary dipterocarp forest. Graphium antiphates Cramer, Ypthima semperaand Ptychandra lorquini-lorquini were found to be endemic to Bulusukan. Butterflies were observed to be attracted to shady forest areas consisting of mosses clinging on metamorphic rocks along a river and includes species from the families Nymphalidae and Papilionidae. This study was able to identify 21 butterfly species with 19 genera 2 of them are rare and 2 endemic species of butterflies in Bulusukan. It is recommended to continue further study for wet and dry season.
Occurrence of whirling disease symptoms in cultured common carp in Basrah, IraqDrNajimRKhamees
A total of 128 fish samples including 70 Cyprinus carpio Linnaeus, 1758,10 Leuciscus vorax (Heckel, 1843), 40 Oreochromis aureus (Steindachner, 1864) and eight Planiliza abu (Heckel, 1843) were collected during the period from November 2016 to May 2017. Seven different localities (floating cages, earthen ponds and natural water) in Basrah were investigated. Among some detected fish diseases, whirling
disease symptoms was reported for the first time in Iraq in April 2017, from two C. carpio cultured in earthen fish ponds of Marine Science Centre, University of Basrah.
The infested fish were dwarfed, with abnormal big head and small body. Grossly signs of the diseases represent fins and tail deformities in addition to open ulcers and losing of scales, and necrosis of skin and muscles. Internally, fish suffered of muscles
ecchymosis and vertebral deformities. Histological sections revealed that the spinal cord had healthy structure.
First report of the parasitic infection in two snail species from Burkina Fas...Open Access Research Paper
Trematodiases are important yet neglected tropical diseases, caused by trematode parasites with a multi-host life cycle, which typically involves a snail intermediate host. The many knowledge gaps regarding the trematode life cycles, pathology, and epidemiology complicate effective control. This work was initiated to inventory parasites as part of the “One Health” initiative, where human and animal trematodes are considered equally important, in order to map their distribution, detect high-risk locations and improve disease control. This paper describes the occurrence of parasitic infections in the Ouagadougou reservoirs. These reservoirs are under intensive market gardening. The infra- and component community of digenetic trematodes and other parasites in a freshwater gastropod community were examined over a 5-month period. A total of 1031 Thiaridae snails was collected. Among them, 109 belonging to 2 species were infected by larval trematodes. Seven different types of cercaria were found: xiphidiocercaria, furcocercous, megalurous cercaria, monostome cercaria, Armatae xiphidiocercaria, echinostome cercaria and gymnocephalus cercaria. In addition to trematode infections, nematode and oligochaete (Chaetogaster limnaei limnaei) infections have been reported during this investigation. The association of Chaetogaster limnaei limnaei with the snail intermediate host may be of value as a control measure against economically important parasitic diseases such as fascioliasis and schistosomiasis..
Studies on the Phyllosoma Larva of the Indian Rock Lobster, Panulirus Homarus...ijtsrd
Attempts were made to develop a technique to rear the phyllosoma larvae of Panulirus homarus. The biological characters like fecundity, hatching percentage, larval morphological changes, feed inputs and moulting frequency till the fourth moult were studied. Morphometric and meristic characters of the larvae were also studied till the 42ndday. The larval output was directly proportional to the size of the gravid brood stock. Relationship between the duration of culture X and length of the larvae Y were shown by the relationships Y intercept = 0.5780 ± 0.1074 and X intercept = 0.7283 r2 = 0.8519 . There was significant p 0.0001 positive relationship between total length TL and carapace width CW of phyllosoma larvae. S. Lazarus | J. C. Nisha | R. Thangaraja "Studies on the Phyllosoma Larva of the Indian Rock Lobster, Panulirus Homarus Linnaeus, 1758" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020, URL: https://www.ijtsrd.com/papers/ijtsrd31676.pdf Paper Url :https://www.ijtsrd.com/biological-science/molecular-biology/31676/studies-on-the-phyllosoma-larva-of-the-indian-rock-lobster-panulirus-homarus-linnaeus-1758/s-lazarus
Head Combs for Delousing in Ancient Arican Populations: Scratching for the Evidence
Peines para el Despiojamiento en las Antiguas Poblaciones de Arica: Rascando la Evidencia
Bernardo Arriaza, Vivien G. Standen, Jorg Heukelbach, Vicki Cassman and Felix Olivares
Journal of Nutrition and Health Sciences is an open access journal that publishes peer reviewed research articles and short communications in all aspects of nutrition. This Journal encompasses the full spectrum of nutritional science including nutritional requirements, public health nutrition, epidemiology, dietary surveys, body composition, energetics, appetite, obesity, ageing and metabolic studies.
Journal of Proteomics & Geneomics (JPG) is an instructional journal providing a chance to researchers and scientists to explore the advanced and latest research developments within the field of Proteomics and Geneomics. Journal of Proteomics & Geneomics publishes the best quality scientific articles amalgamating broad vary of fields together with the fields associated with Proteomics & Geneomics.
Journal of Obesity and Overweight (JOO) is a peer reviewed open access journal. It is dedicated to increase knowledge, fostering research, and promoting better treatment for people with obesity. It includes subjects like nutrition medicine, clinical nutrition medicine, genetics and nutrition, biophysics and lipid metabolism, etc. It aims to publish advanced research works related to public health and medical developments.
Journal of Computational Systems Biology (JCSB) is an open access online journal which aims to publish peer reviewed research articles and short communications in all aspects of computational biology and bioinformatics. JCSB comprehend the broad spectrum of computational bioscience including biological databases and bioalgorithms.
Journal of Gynecology Research (JGR) publishes original articles and research studies on, scientific advances, new medical and surgical techniques, obstetric management, and clinical evaluation of drugs and instruments and all aspects of gynecology including gynecological endoscopy, infertility, oncology contraception, urogynecology, fertility, and clinical practice and ultrasonography. It aims to publish the highest quality medical research in women's health, worldwide.
CLINICAL AND EXPERIMENTAL RESEARCH IN CARDIOLOGYAnnex Publishers
Journal of Clinical and Experimental Research in Cardiology (JCERC) is an international open access, scholarly peer-reviewed journal publishing high quality articles in all areas of cardiology related fields, especially current research, new concepts, novel methods, new therapeutic agents, and approaches for early detection and prevention of cardiac disorders and reporting new methods on basic and advanced clinical aspects of cardiology research.
Journal of Genetic Mutations and Disorders (JGMD) is an open access, peer reviewed journal which provides advanced researches including Genetics of Infectious Diseases, Genealogical Tracing, Stem Cell Research, Gene mapping with three-point crosses, Genetic linkage and genetic maps. JGMD publishes original research, review articles in all aspects of genetic mutations and disorders
CLINICAL AND EXPERIMENTAL RESEARCH IN CARDIOLOGYAnnex Publishers
Journal of Clinical and Experimental Research in Cardiology (JCERC) is an international open access, scholarly peer-reviewed journal publishing high quality articles in all areas of cardiology related fields, especially current research, new concepts, novel methods, new therapeutic agents, and approaches for early detection and prevention of cardiac disorders and reporting new methods on basic and advanced clinical aspects of cardiology research.
Journal of Bioequivalence Studies (JBS) is an open access, peer reviewed journal that publishes the most relevant and reliable researches with respect to the subject of Bioequivalence studies which includes pharmacokinetic and pharmcodynamic properties of a drug. JBS publishes original articles, review articles, case reports, short communications, etc.
Journal of Forensic Science & Criminology (JFSC) is an open access, significant and reliable source of contemporary knowledge on advancements in the field of forensic science. JFSC publishes peer reviewed research articles, critical reviews and short communications focused on forensic science and criminology. JFSC encompasses the full spectrum of forensic science including forensic biology, forensic chemistry, cyber forensics and crime scene investigation
Anti arthritic-efficacy-and-safety-of-crominex-3+(trivalent-chromium-phyllant...Annex Publishers
Abstract
The present investigation was undertaken to evaluate the therapeutic efficacy and safety of Crominex® 3+ (a complex of trivalent chromium, Phyllanthus emblica (Amla) extract and purified Shilajit) in moderately arthritic dogs. Eleven client-owned moderately arthritic dogs in a randomized double-blinded study received placebo or Crominex® 3+ twice daily for a period of 150 days. On a monthly basis, each dog was evaluated for arthritis associated pain (overall pain, pain upon limb manipulation and pain after physical exertion) and a full physical exam (body weight, body temperature and heart rate). At the same time intervals, dogs serum samples were examined for biomarkers of kidney (BUN and creatinine), liver (bilirubin, ALT and AST) and heart and skeletal muscle (CK) functions. Findings of this investigation revealed that dogs receiving Crominex® 3+ (1000 μg chromium, 15 mg Amla extract and 15 mg purified Shilajit per day in two divided doses) exhibited a significant (P< 0.05) reduction in arthritic pain noted as early as after 90 days with a maximum reduction after 150 days of treatment. Pain level remained the same or slightly increased in the dogs receiving placebo. No significant change occurred in physical parameters or serum biomarkers in dogs on placebo or Crominex® 3+, which suggested that Crominex® 3+ was well tolerated by arthritic dogs. In conclusion, Crominex® 3+ significantly (P< 0.05) ameliorated arthritic pain and improved quality of life without causing any untoward effects in moderately arthritic dogs.
Abstract
The objectives of this retrospective study were 1. To determine the effect of three surgical techniques (right flank omentopexy, right flank omentoabomasopexy, and left flank abomasopexy), and 2. To determine the effect of concurrent disease on return to normal milk production. Return to normal milk production occurred in 86.3% of cows diagnosed with LDA. Results suggested that cattle diagnosed with LDA corrected via right flank omentopexy or left flank abomasopexy were significantly more likely to return to normal milk production as compared to those corrected via right flank omentoabomasopexy (p< 0.02). No significant difference in return to normal milk production was noted between surgical techniques for correction of RDA (p=1.000) and right abomasal volvulus (p=0.596). Concurrent disease diagnoses did not affect return to milk production. Reported complications were infrequent (n=11).
List of abbreviations: LDA- Left displaced abomasum; RDA- Right displaced abomasum; RAV- Right abomasal volvulus; RAOV- Right abomasal-omasal volvulus; RFO- Right flank omentopexy; RFOA- Right flank omentoabomasopexy; LFA- Left flank abomasopexy; DA – Displaced Abomasum
Abstract
Three surgical case reports are presented to demonstrate the clinical efficacy of using an improved aqueous solution of chlorine dioxide complex (160 ppm) as a topical antiseptic in the post operative management of serious wounds in dogs. In vitro studies are included to demonstrate the antiseptic properties of this new chlorine dioxide complex.
Keywords: Chlorine dioxide; Antiseptic; Antimicrobial; Wound management
Abbreviations: ClO2-Chlorine dioxide; Cl2-Chlorine; PPM-Parts Per Million; SPP-Species; TEM-Transmission Electron Micrograph
The updated-international-veterinary-anatomical-and-embryological-nomenclaturesAnnex Publishers
The international nomenclature of the anatomical, histological and embryological terms is known as Nomina Anatomica Veterinaria (N.A.V.), Nomina Histologica Veterinaria (N.H.V.) and Nomina Embryologica Veterinaria (N.E.V.).
This is the tripod of terms for the morphological sciences in our profession, a dictionary of terms used by all specialists in the basic and in the clinical sciences.
The discovery of the presence of stem cells and precursors with high regenerative potential in the mammary gland, hypothetically maintained throughout the course of the productive life of the dairy cow sheds an interesting perspective in the research which is interested to clarify all physiological clues and possible solutions to increase or maintain longer the potential production of dairy cows during life span of lactation. In 2006, Connie Eaves' lab in Vancouver (Stingl et ., 2006) [1]
Abstract
Mitogen-Activated Protein Kinase (MAPK) pathway is a signal transduction pathway that functions in a wide range of physiological and pathophysiological cellular events including cell proliferation, differentiation, apoptosis, migration, inflammation, metabolic disorders and diseases. In skeletal muscle, it plays an essential role in muscle fiber specialization, muscle mass maintenance, damage induced muscle regeneration and muscle diseases. This review provides an overview of MAPK pathway and its pathophysiological role in skeletal muscle diseases with a primary focus on muscular dystrophy and atrophy.
Abstract
An examination experiment has been carried out to investigate whether fingerprint powder and lifting technique can recover and transfer latent fingerprints from human skin surfaces of dead bodies. For recovery Swedish Black powder and for transfer White Fingerprint Gelatine were used.
Donors placed fingerprints on the human skin surfaces. Finger marks were then in all cases recovered with Swedish Black powder. The procedure was repeated after 2, 3, 4 and 5 hours. Treated finger marks were secured and preserved as latent fingerprint evidence by lifting process. We recovered 8% finger marks suitable for further examination of all deposited samples on the human skin surfaces.
The fatal-alcoholic ketoacidosis-as-a-dilemma-in-the-differential-diagnosis-i...Annex Publishers
48-years old man was found dead at home, lying in his bed. There was a bucket with liquid vomited remnants with no addition of fresh or digested blood found near the bed and an empty bottle of alkaline mineral water often used to attenuate the gastric pain. Up to the information from the man's relative, he was a chronic alcohol consumer, being last seen alive the day before in the pub, drinking small amount of beer. His personal anamnesis was negative for the previous injuries or any pathological entity which could have potential fatal outcome. External observation of the body did not uncover any signs of mechanical violence on the body. The only pathological findings at the autopsy were: very mild generalised atherosclerosis without stenosis of blood vessels, configuration of the heart with the weight of 400 g was physiological, with no signs of chronic venostatic changes on the internal organs.
Journal of Forensic Science & Criminology (JFSC) is an open access, significant and reliable source of contemporary knowledge on advancements in the field of forensic science. JFSC publishes peer reviewed research articles, critical reviews and short communications focused on forensic science and criminology. JFSC encompasses the full spectrum of forensic science including forensic biology, forensic chemistry, cyber forensics and crime scene investigation
Abstract
The synthetic cannabinoid, N-(1-adamantyl)-1-pentyl-1H-indazole-3-carboxamide, known also as AKB48 and/or APINACA, has been detected for the first time in herbal incense seized in Italy. Its structural characterization has been performed through gas chromatography-mass spectrometry (GC-MS) and high-resolution nuclear magnetic resonance (NMR) analysis. In order to favor an easier and faster identification of AKB48 in future investigations, NMR assignments in deuterated methanol and chloroform is also reported.
Keywords: NMR; GC-MS; Designer drug; Synthetic cannabinoid; Herbal incense
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
The flesh-fly-sarcophaga-(liopygia)-crassipalpis-macquart-1839-as-an-invader-of-a-corpse-in-calabria-(southern-italy)
1. Annex Publishers | www.annexpublishers.com
Volume 1 | Issue 4Abstract
We present an indoor forensic case that occurred in spring 2013 in Cosenza (southern Italy). The entomological evidence collected at the scene consisted of Calliphoridae (Calliphora vicina, Lucilia sericata), Sarcophagidae (Sarcophaga crassipalpis), Fanniidae (Fannia scalaris) and Muscidae (Hydrotaea ignava). The minimum Post Mortem Interval (mPMI) was calculated by relating the entomological evidence to data available for Diptera species in the area and to our knowledge of the development of flies used as forensic indicators in Calabria. We report S. crassipalpis as a corpse invader for the first time in Italy. Introduction
The Flesh Fly Sarcophaga (Liopygia) crassipalpis Macquart 1839 as an Invader of a Corpse in Calabria (Southern Italy)
Bonacci T*1, Greco S1, Cavalcanti B2, Brandmayr P1 and Vercillo V2
1Department DiBEST, University of Calabria, 87036 – Rende (CS), Italy
2Azienda Sanitaria Provinciale di Cosenza, Sezione di Medicina Legale, 87100 – Cosenza, Italy
*Corresponding author: Bonacci T, Department DiBEST, University of Calabria, 87036 – Rende (CS), Italy, E-mail: teresa.bonacci@unical.it
Citation: Bonacci T, Greco S, Cavalcanti B, Brandmayr P, Vercillo V (2014) The Flesh Fly Sarcophaga (Liopygia) crassipalpis Macquart 1839 as an Invader of a Corpse in Calabria (Southern Italy). J Forensic Sci Criminol 1(4): 404. doi: 10.15744/2348-9804.1.404
Research Article
Open Access
Volume 1 | Issue 4
Journal of Forensic Science & Criminology
Keywords: Forensic case; Flies; S. crassipalpis; mPMI; Southern Italy
The first aim of forensic entomology is to help investigators estimate the time of death. Its application has become widespread in many countries because the entomological evidence on corpses (especially Diptera and Coleoptera) can be used to estimate the minimum Post Mortem Interval (mPMI) at both indoor and outdoor crime scenes. The arthropod community discovered on a corpse or around it is affected by extrinsic and intrinsic factors [1]. The geographical area, the season and the habitat are very important in determining the composition of the faunal succession and the seasonal dynamics of insects [2,3] on exposed corpses. Knowledge of the distribution and ecological preferences of arthropod forensic indicators in a particular area [4-7] is very useful for PMI estimation and crime scene analysis. In particular, synanthropic flies, the most accurate indicators of the time of death, have been recognized on human corpses in many countries [8,9]. Until recent investigations in Calabria, southern Italy [4-7], investigators evaluating the insects collected at crime scenes in that region based their conclusions on data recorded mainly from central Europe. In the present case, five species belonging to the families Calliphoridae (Calliphora vicina Robineau-Desvoidy 1830, Lucilia sericata (Meigen 1826)), Sarcophagidae (Sarcophaga (Liopygia) crassipalpis Macquart 1839), Fanniidae (Fannia scalaris (Fabricius 1794)) and Muscidae (Hydrotaea ignava (Harris 1780)) were collected at the scene. In particular, we discussed the presence of S. crassipalpis: this is the first time the species has been collected on a corpse in Italy. mPMI was estimated on Calliphora vicina samples as discussed in the result section.
On May 24, 2013, the body of a 50-year-old male was found on the floor of a room in Cosenza (southern Italy) (Figure 1A) which did not receive direct sunlight. At the time of discovery, the door was shut but two windows of the apartment were open. The temperature in the apartment when the corpse was discovered was 22.5 °C. The cadaver was found in a supine position dressed in a shirt and trousers. The feet were completely bare. Medical evidence and a toxicological analysis carried out on the cardiac blood confirmed that death was associated with methadone intake. The methadone concentration in the blood was 242 ng/ml. The body was in an advanced state of decay, with heavy maggot infestation on the face (Figure 1B) and on the entire body. Entomological evidence was abundant and present on and around the body. The procedures for collecting and processing the entomological evidence followed normal standards and guidelines [10,11]. At the autopsy on 25 May, the corpse showed general brownish discoloration and autolysis of internal organs, signs of advanced decay. Macroscopic investigations showed superficial skin lesions on the wrists, fluid and dark blood, plurivisceral congestion, hypertrophic chronic cholecystitis with cholesterolosis. Microscopic Case description
Received Date: December 03, 2013 Accepted Date: February 10, 2014 Published Date: February 12, 2014
ISSN: 2348-9804
2. Annex Publishers | www.annexpublishers.com
Journal of Forensic Science & Criminology
2
Volume 1 | Issue 4
investigations of the lungs showed widespread spill, areas with alveolar spaces occupied by eosinophilic edema, red blood cells and polymorph nuclear leucocytes, and areas of emphysema. The heart showed several areas of coagulative necrosis with myofibrillar suffering. The brain showed hydrophic hypoxic degeneration and the liver showed chronic active hepatitis.
Figure 1: A. View of the room where the corpse was discovered
B. Maggots at different life stage inside the natural orifices
Several dead adult specimens of Calliphora vicina Robineau-Desvoidy, 1830, Lucilia sericata (Meigen, 1826), Fannia scalaris (Fabricius 1794) and Hydrotaea ignava (Harris 1780) were found and collected around the corpse and in the bathroom next to the room with the corpse. Many maggots were observed and collected on the corpse (in the clothing, nose, mouth, eyes, ears) and around it. Sixty larvae (45 third instar larvae of C. vicina and 15 second and third instar larvae of L. sericata) were isolated and stored in 80% ethanol, while 30 larvae of C. vicina and 10 larvae of L. sericata were reared in the laboratory to the adult stage. The adults and larvae were identified using identification keys [12-17]. The larvae were killed by immersion in actively boiling water for 60 s and transferred to 80% ethanol for preservation [18]. Both the larvae and the adults were identified as C. vicina and L. sericata. From the sample reared in laboratory are not emerged adults of F. scalaris and H. ignava. In total, 50 third instar larvae of Sarcophaga (Liopygia) crassipalpis Macquart 1839 were collected on the body, although adults of this species were not found at scene. Thirty of these larvae were killed by immersion in actively boiling water for 60 s and transferred to 80% ethanol for preservation [18], while the other 20 larvae were reared in the laboratory to the adult stage at 20 ± 0.5 °C. Both the larvae and adults were identified as S. Results
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crassipalpis (Figure 2) using identification keys [14]. All the examined larvae are deposited in the ‘Tullia Zetto larval collection’ (TZC) while the adults are deposited in the collection of Prof. Pietro Brandmayr (PBC) (both DiBEST Department, University of Calabria, Cosenza, Italy). Digital photographs of cadaver were taken with a Sony Super Steadyshot DSC-H2 digital camera. The larvae and adults of species were photographed with a Zeiss Axioskop equipped with Nomarski optic (Differential Interference Contrast, DIC), using a Nikon Coolpix 4500 four MP digital camera.
Figure 2: Sarcophaga (Liopygia) crassipalpis Macquart 1839.
A. Habitus of male (scale bar: 1 cm).
B. Male genitalia, lateral view (scale bar: 1 mm).
C. Male genitalia, dorsal view (scale bar: 1 mm).
D. Cercus and surstylus in frontal view (scale bar: 1 mm).
E. Cercus and surstylus in lateral view (scale bar: 1 mm).
F. Cephalopharyngeal skeleton of third instar larva (scale bar: 0.5 mm).
G. Posterior spiracles of third instar larva (scale bar: 0.25 mm).
H. Anterior spiracles of third instar larva (scale bar: 0.15 mm).
Soft and clear pupae of Calliphora vicina were collected on the blanket covering the sofa, under the sofa (Figure 1A) and near the body. In this case, C. vicina pupae were used for mPMI estimation. Empty puparia were not found at the scene. In order to verify the differences between the temperature at scene and from weather stations we used an Escort Junior data logger which recorded in the apartment the temperature at 30-min intervals for 5 days after discovery of the body. Temperature data for the 10 days before discovery of the corpse were obtained from the local weather stations of the Arpacal Agency of Calabria situated 800 meters from the scene. To determine the mPMI, we used the temperatures (Min and Max) recorded before discovery of the body. The mean environmental temperature recorded during the 10 days before discovery was 21.5 ± 1.1 °C. Laboratory evaluation aimed to study the growth of C. vicina larvae which fed on meat with added methadone not allow general statements concerning the determination of
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the age of larvae which developed under the influence of methadone and the effective influence of substance on the development time of the blow fly [19]. According to Marchenko [20] and unpublished data from our laboratory, the mean minimal development time from egg to pupal age of C. vicina at 22 °C is 9.6 ± 0.2 days; the average minimal development time from egg to pupal age of C. vicina at 23 °C is 9.1 ± 0.2 days. Based on the time of development (egg-pupal age) determined at 22 °C for this species under laboratory conditions [20], the expected accumulated degree-day (Exp. ADD) was 192. Based on the time of development (egg- pupal age) determined at 23 °C, the expected accumulated degree-day (Exp. ADD) was 191. For C. vicina a lower threshold of 2 °C was applied [20]. This lower threshold is in accordance with our laboratory experimental data (unpublished data) for C. vicina. Therefore, it was estimated that oviposition of C. vicina took place somewhere between 14 and 15 May.
In recent years there has been a progressive increase in mortality rates from acute methadone poisoning. This can be related to the increasing diffusion of recovery programs, particularly those entrusting the methadone administration directly to the user. This practice involves the likelihood of mistakes of self-administration by patients, i.e. too high doses or methadone combined with other central nervous system depressants. In extreme cases, methadone can be used to commit suicide [21]. With regard to necrophagous fauna, many authors have reported that the number of Diptera species colonizing cadavers in domestic environments is lower than outdoors [22]. Our investigation of an indoor scene in the city of Cosenza revealed a massive colonization by flies. In fact, we collected five necrophagous Diptera species from and around the body. In Calabria, Calliphora vicina and Lucilia sericata are the most abundant synanthropic species [7]. C. vicina is active in Calabria in spring, autumn and winter, while L. sericata is active in spring and summer [7]. Hydrotaea ignava is known to be associated with carcasses in spring and summer in other geographical areas [23] and in Cosenza province [5,6]. Fannia scalaris is associated with human corpses and frequently indicates the presence of feces and urine in indoor environments [24]. In Calabria this species was reported from pig carcasses in spring and summer [5]. The presence at the scene of the flesh fly S. crassipalpis (Figure 2) is interesting. This species has been reported to cause myiasis in humans and animals (oral, intestinal, cutaneous and ophthalmic myiasis) [25-28] and has successfully been reared from pork liver [29]. This fly is known to complete larval development in vertebrate and invertebrate carcasses [30-32]. S. crassipalpis has been reared not only from dead vertebrates but also from dead invertebrates, particularly snails [33]. Adults feed on feces, carrion and flowers. This fly is defined as a hemisynanthropic species, present in tropical and subtropical zones of all biogeographical regions [33] and widely distributed in Spain, where it has also been observed associated with corpses [29,35,36]. S. crassipalpis is also abundant in Portugal, with a clear preference for sunny sites [24]. Our finding is the first report of S. crassipalpis associated with a human corpse at an indoor scene in Italy. In Calabria, S. crassipalpis shows synanthropic preferences and can also be associated with corpses located in shady conditions contrary to what has been observed in other geographical areas [24]. Conclusion
The large number of species collected on the body and at the scene, related to the season and the geographical area, underline the importance of evaluating entomological evidence on a regional scale. The massive presence of S. crassipalpis strongly suggests that the species can be considered a forensically relevant fly for indoor scenes in Calabria, like the other species (Calliphora vicina, Lucilia sericata and Chrysomya albiceps) [4-6]. These species can be defined as early colonizers and are of potential use for the estimation of mPMI in southern Italy.
We authors sincerely thank the Comando Provinciale Carabinieri of Cosenza for the support to this study. We thank Dr. Daniel Whitmore (Curator (Diptera) - Department of Life Sciences Natural History Museum, London) for confirming our identification of the specimens of Sarcophaga crassipalpis. Thanks to anonymous reviewers for their critical review of the manuscript. AcknowledgementsReferences
1. Campobasso CP, Di Vella G, Introna F (2001) Factors affecting decomposition and Diptera colonization. Forensic Sci Int 120: 18-27.
2. Schroeder H, Klotzbach H, Püschel K (2003) Insect’s colonization of human corpses in a warm and cold season. Leg Med 5: S372-4.
3. Anderson GS (2009) Factors that influence insect succession on carrion. Forensic entomology: The utility of Arthropods in legal investigations (2nd edn), Byrd JH and Castner JL, CRC Press, Florida, United States.
4. Bonacci T, Vercillo V, Brandmayr P, Fonti A, Tersaruolo C, et al. (2009) A case of Calliphora vicina Robineau-Desvoidy, 1830 (Diptera, Calliphoridae) breeding in a human corpse in Calabria (southern Italy). Leg Med: 11: 30-2
5. Bonacci T, Brandmayr P, Greco S, Tersaruolo C, Vercillo V, et al. (2010) A preliminary investigation of insect succession on carrion in Calabria (southern Italy). Terr Arthropod Rev 3: 97-110.
6. Bonacci T, Zetto Brandmayr T, Brandmayr P, Vercillo V, Porcelli F (2011) Successional patterns of the insect fauna on a pig carcass in southern Italy and the role of Crematogaster scutellaris (Hymenoptera, Formicidae) as a carrion invaders. Entomological Science 14: 125-32.
7. Greco S, Brandmayr P, Bonacci T (in press) Synanthropy and temporal variability of Calliphoridae living in Cosenza (Calabria, South Italy). Journal of Insect Science (in press).
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8. Nuorteva P (1977) Sarcosaprophagous insects as forensic indicators. Forensic Medicine: A Study in Trauma and Environmental Hazards, Vol. II: Physical Trauma, eds. Tedeschi, et al., W. B. Saunders Co., Philadelphia, London, Toronto.
9. Smith KVG (1986) A manual of forensic entomology. The Trustees of the British Museum (Natural History), Cornell University Press, London, Ithaca.
10. Haskell NH, Catts EP (1990) Entomology and Death: A procedural guide, Joyce’s Print Shop, Clemson, SC: 52-97.
11. Amendt J, Campobasso CP, Gaudry E, Reiter C, LeBlanc HN, et al. (2007) Best practice in forensic entomology-standards and guidelines. Int J Legal Med 121: 90-104.
12. Whitworth (2010) Keys to the genera and species of blow flies (Diptera: Calliphoridae) of the West Indies and description of a new species of Lucilia Robineau- Desvoidy. Zootaxa 2663: 1-35.
13. Szpila K (2010) Key for the identification of third instars of European Blowflies (Diptera: Calliphoridae) of forensic importance. Current Concepts in Forensic Entomology, edited by Amendt et al., Springer Science+Business Media.
14. Richet R, Blackith RM, Pape T (2011) Sarcophaga of France (Diptera: Sarcophagidae). Pensoft, Sofia_Moscow: 327.
15. Pape T (1987) The Sarcophagidae (Diptera) of Fennoscandia and Denmark. Fauna Entomol Scand 19: 1-203.
16. Pape T (1996) Catalogue of the Sarcophagidae of the world (Insecta: Diptera). Mem Entomol Int 8: 1-558.
17. Gregor F, Rozkošný R, Barták M, Vaňhara J (2002) The Muscidae (Diptera) of Central Europe. Folia Facultatis Scientiarum Naturalium Universitatis Masarykianae Brunensis, Biologia 107: 1-280
18. Adams ZJO, Hall MJR (2003) Methods used for the killing and preservation of blowfly larvae, and their effect on post-mortem larval length. Forensic Sci Int 138: 50-61.
19. Strehler M, Klotzbach H, Madea B, Relbe S (2008) Development of Calliphora vicina under food addition of methadone. Legal Medicine 18: 425-30.
20. Marchenko MI (2001) Medicolegal relevance of cadaver entomofauna for the determination of the time of death. Forensic Sci Int 120: 89-109.
21. Tennant F (2007) Methadone deaths and warnings. Practical pain management 7: 8-9.
22. Anderson GS, VanLaerhoven SL (1996) Initial studies on insect succession on carrion in southwestern British Columbia. J Forensic Sci 41: 617-25.
23. Schröder H, Klotzbach H, Oesterhelweg L, Gehl A, Püschel K (2001) spectrum of species and temporal occurrence of insects flats corpses in the Hamburg area. Legal Medicine 11: 59-63.
24. Prado e Castro C, Garcia MD, Arnolds MI, González-Mora D (2010) Sarcophagidae (Diptera) attracted to piglet carcasses including new records for Portuguese fauna. Graellsia 66: 285-94.
25. Benecke M (2004) Forensic entomology: Arthropods and Corpses. Forensic Path Rev Vol II, Tsokos M, Humana Press, Totowa, New Jersey, USA.
26. Morris B (1987) First reported case of human aural myiasis caused by the flesh fly Parasarcophaga crassipalpis (Diptera: Sarcophagidae). J Parasitology 73: 1068-9.
27. Shiota T, Yoshida Y, Hirai S Torii S (1990) Intestinal myiasis caused by Parasarcophaga crassipalpis (Dipera: Sarcophagidae). Pediatrics 85: 215-7.
28. Shinonaga S, Kurahashi H (1969) New species of Sarcosolomonia Baranov from New Guinea and the Solomons (Diptera: Sarcophagidae). Pacific Insects 11: 755-61.
29. Martínez-Sánchez A, Magaña C, Pérez-Bañón C, Rojo S, González-Mora D (2006) Taxonomy and larval study of flesh flies (Diptera: Sarcophagidae) associated with human corpses in Spain. Proceedings of the Fourth Meeting of European Association for Forensic Entomology, Bari: 63 [abstract].
30. Sanjean J (1957) Taxonomic studies of Sarcophaga larvae of New York with notes on the adults. Cornell University Agricultural Experiment Station Memoir 349: 1-115.
31. Oliva A (2001) Insects of forensic significance in Argentina. Forensic Sci Int 120: 145-54.
32. Centeno N, Maldonado M, Oliva A (2002) Seasonal patterns of arthropods occurring on sheltered and unsheltered pig carcasses in Buenos Aires province (Argentina). Forensic Sci Int 126: 63-70.
33. Pérez-Moreno S, Marcos-García MA, Rojo S (2006) Comparative morphology of early stages of two Mediterranean Sarcophaga Meigen, 1826 (Diptera; Sarcophagidae) and a review of the feeding habits of Palaearctic species. Micron 37: 169-79.
34. Povolny´ D, Verves YG (1997) The flesh-flies of Central Europe (Insecta, Diptera, Sarcophagidae). Spixiana Supplement 24, Munich, Germany.
35. Miralbes Castillo M (2002) Study of the insect fauna associated with carcasses in the Alto Aragón (Spain). Monographs of the Entomological Society Aragonese 6: 1-94.
36. Romera E, Arnaldos MI, García MD, González-Mora D (2003) Los Sarcophagidae (Insecta, Diptera) de un ecosistema cadavérico en el sureste de la Península Ibérica. Anales de Biología 25: 49-63.