1) Ingole R. S., Khakse H. D., et.al. ‘INCIDENCE OF HYDATIDOSIS IN SLAUGHTERED CATTLE, BUFFALOES AND GOATS’ Published in Abstract Compendium of 31st Annual Conference of Indian Association of Veterinary Pathologist, National Symposium on “Impact of Climate Change on Pathology of Disease of Animals, Poultry and Fish” 13th to 15th Nov. 2014, organized by Anand Agriculture University, Anand, Sr. No. 41, Page 86. 2) Ingole R. S., Khakse H. D., et.al. ‘MOLECULAR CHARACTERIZATION OF ECHINOCOCCUS GRANULOSUS IN ANIMALS BY SEQUENCING OF COX-1 GENE’ Published in Abstract Compendium of 31st Annual Conference of Indian Association of Veterinary Pathologist, National Symposium on “Impact of Climate Change on Pathology of Disease of Animals, Poultry and Fish” 13th to 15th Nov. 2014, organized by Anand Agriculture University, Anand, Sr. No. 28, Page 86. 3) Ingole R. S., Khakse H. D., et.al. ‘PREVALENCE OF ECHINOCOCCUS GRANULOSUS IN DOGS BY POLYMERASE CHAIN REACTION’ Published in Abstract Compendium of 31st Annual Conference of Indian Association of Veterinary Pathologist, National Symposium on “Impact of Climate Change on Pathology of Disease of Animals, Poultry and Fish” 13th to 15th Nov. 2014, organized by Anand Agriculture University, Anand, Sr. No. 29, Page 86.

1. VETERINARY PATHOLOGY CONGRESS . 2014
onferenco*tf lrdia* Associatioq of Veterinry Pdholqgists3lst Annual"l
5th Annual Meetirry of Indian College of Veterinary Pathologists, !l
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Anand Agricultural University,
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41. INCIDENCE OF HYDATIDOSIS IN SLAUGIITERED CATTLE,
BUFEALOES AND GOATS
R S. Ingole, H.D. Khakse, M. G. Jadhao, M S. Hedau, N. M. Bhojane and M. V. Joshi
Depafrnent of Veterinary Pathology, Post Graduate Institute of Veterinary and Animal Sciences,
Krishi Nagar, Akola- 444104 (MS)
Hydatidosis is a serious parasitic cyclo zoonotic disease caused by larval stage (metacystode) of
dog tapeworm Echinocaccus granulosas considered to be an important condition in herbivoris socfi as
cattle,'buffaloes, sheep and goats. In livestock hydatid disease leads to reduced yield, quality of meat,
milk and birth rate. Economic losses are also occurring due to condemnation of organs, especiaily of liver
and lung in slaughtered food animals. The study was undertaken to record the prevalence of hydatidosis
in cattle, buffaloes and goats of Akola distict of Maharashtra. For the present study total 216i animals
which include 1881 cattle, 50 buffaloes and 230 goats slaughtered in seven tahsils of Akola disfrict were
observed for post mortem examination during slaughter. The overall incidence of hydatidosis was recorded
highest in buffaloes (8.00 %) followed by cattle (3.99 % On the basis of Tahsil wise analysis, the highest
prevalence for hydatidosis was recorded in Balapur tahsil followed by Akola, Akot, Murtrjapur, Barshi
Takali, Patur and Telhara for cattle and buffalo both. The older animals having age group of 8 - 10
years or more are found more predominantly infected. Irespective of host species, the hydatid cysts were
predominantly found in lung and liver. The majority of individual animals harboured hydatid cysi within a
single organ either lung or liver. Lungs (77.33%) followed by liver (20%) were found to be most preferred
site for localization of hydatid cyst in cattle, however in buffaloes liver (75 %) was most preferred site
for localization. only one bullock kidney (1.33 %) was the site of hydatid cysl.
42. PATHOBIOLOGY OF CYSTIC OVARIAN DISEASES IN CATTLE
Deparrnent of veterinary *il'i?"H'!lii;#"#T:,3ffiHtr#3"*:::it*11,'"+"rabhnagar, udaipur (Raj.)
The present investigation was carried out from May 2009 to October 2009. During this period a total
number of 390 samples of female genital tract were examined from cattle of different age and breeds.
Out of these 55 samples showing gross lesions were collected and tissue sections from these were sub-
jected to histopathological examination, In the present study the overall occlurence of Cystic ovarian
disease in genital tract of cattle is found to be 10.25% (1,6 cases), out of which Follicular cyst is 9.6L%
(15 cases) and Cystic corpus luteum is 0.64% (1 case).
43. A CASE REPORT OF NASAL GRANULOMA IN DONKEY
Deparnnent
jA?$:H&t"*;,fff3kl:?i,#l* J;,1;," and A H,
Junagadh Agricultural University, Junagadh
A twelve year male donkey was brought to Teaching veterinary Clinical complex, Junagadh
Agricultural University, Junagadh with history difficulty in breathing since last trvo months. On detiiled
clinical examination revealed the polyps like growth in the nasal mucosa. The surgery was carried out
by taking all the aseptic measures. The biopsy material was subjected to histopathology. The microscopic
examination revealed the bunches of eosionophils covered by fibrous connective tissue and might be due
to allergic reaction.
Orgonizcd by Deporlment of Veterinory Pothology, Veterinory College, AAU, Anond, Guiorot i
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28. MOLECULAR CHARACTERIZATION OF ECHINOCOCCUS GRANULOSUS IN
ANTMALS By SEQUENCTNG OF COX_I GENE
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Krishi Nagar, Akola- 444 104.
Cystic echinococcosis caused by metacestode stage of Echinococcus grnulosus is one of the
important zoonotic disease affecting human and ungulate animals worldwide. ih. dir.u.. causes great
economic losses through condemnation of infected organs, in particular liver and lungs. At presej ten
genotypes (Gl - G10) have been identified based on genetic diversity. Identification ofltrain
'types
of E.
granulosus will have immense impact on planning for iontol of hydatidosis, hence the study was ptanned
to determine the genotypes present in Akola oistrict of Maharashtra. For the present study total 2334animals which include 1970 cattle, 74 buffaloes,284 goats and 04 sheep *.r" obr.*.d during necropsyexamination and slaughter house inspection in seven iattsits of Akola dirti.t. The hydatid cyst samplesfrom cattle (s5) and buffaloes (6) and goat (2) were collected. The genomic owe was extracted usingprotoscolices or germinal layer and a region of nq bp of the mitocho-ndriur.ytocrrro,oe oxidase-l lcoxll was amplified from each isolate using previously published primer pairs. iie resutting polymerasechain reaction products of 23 isolatr, *ir" s.quen"ed and further examined by sequence analysis. onphylogenetic analysis, out of 23 isolates, 16 (6i.56%) isolates o{ gattle and, 2'(gis9lo) isolates of goatclustered within the Indian Buffalo (G3) strain of E. granulosus, while 2 (g.69%) isolates clustered withinsheep shain (G2)' These findings can be taken into cJnsideration for development of preventive measuresand control of hydatid disease in Akola district of Maharastra.
29. PREVALENCE OF ECHINOCOCCAS GRANALOSU,S IN DOGS
BY POLYMERASE CHAIN REACTION
o*",*'tii+:,:",|if ?*ryffi,:'f#?*#:k,y;.";iitr,##;x;"1,'li,ciences,
Krishi Nagar, Akola_ 444104 (MS)
cystic hydatid disease (cHD) or echinococcosis is a chronic helminthic zoonotic disease causedby metacestode infection of dog tapworm Echinococcu, gro*lorzs. It is tansmitteo cyclically betweencanines and numerous herbivorous livestock animals, wrr'ich can serye as intermediate host. The sfudywas undertaken to record the prevalence of.Echinococcus gron loru, wolm infection in dogs of Akoladistrict of Maharashtra' Total t48 fecal samples of stay dogs-from different tahsils of Akola district whichincludes Akola (60), Balapur (26), Murtijapur (26), etot 1is;, parur (10) and relhara (ll) were collectedfor observing the prevalence of i. gronlloruiin'iogs. ln fi.ri*inary diagnosis by routine conventionalfloatation method no egg of E' granilos,s was observid microscopicaity. eicept one, arr the fecal sampleswere found negative for E' granulosus by PCR. However, only one flcal sample collected from nearbyAkola Munciple slaughter house was found positive for iichinococcus granurosr.ls against mitochondrialcytochrome oxidase-l lcox l] gene. Hence, the overall prevalence of"Echonoioc"u, gron loszs in dogwas recorded as 0'67 percent in Akola district. Determination of E. granulosr;ifl, pcR in dog faeces
f$:JhXtnffilffiI.egs
of E' granutosr *i.'o'.ofirurrv .ouro indicate the potentiat risk of dog
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i orsonized by Daportmenr of veterinory pothorogy, veterinory coilegc, AAU, Anond, Guiorot
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