COMPLEMENT - A group of serum proteins which can be activated (= "fixed") by antigen-antibody complexes or other substances, which may result in lysis of a microbial target, or a variety of other biological effects important in both innate and adaptive immunity. (The majority of these proteins are produced by the liver.)The complex of serum proteins known as COMPLEMENT plays key roles in the lytic and inflammatory properties of antibodies. The CLASSICAL pathway is initiated
by antigen-antibody complexes (via complement components C1, C4, and C2), while the activation of the ALTERNATE pathway (via components B, D and P), and the MBLECTIN ("mannan-binding lectin") pathway may be initiated by other substances independently of adaptive immune responses; all three pathways share those complement components involved in the inflammatory and lytic consequences, namely C3, C5, C6,
C7, C8 and C9. The INFLAMMATION which is a consequence of complement fixation is illustrated by the manifestations of SERUM SICKNESS, and complement is also seen
to be central to the normal process of clearing immune complexes, which is important in preventing IMMUNE COMPLEX DISEASE.
introduction of adaptive immunity. classification of adaptive immunity, factor affecting it and mechanism of adaptive immunity comparison between adaptive immunity and innate immunity. characteristic of adaptive immunity . cell mediated immune responses immunoglobulins
types of immunoglobulins. functions of immunoglobulins, hypersensitivity reactions
The complement system is a part of the immune system that helps or complements the ability of antibodies and phagocytic cells to clear pathogens from an organism. It is part of the innate immune system, which is not adaptable and does not change over the course of an individual's lifetime.
consists of three pathways: 1. alternative
2. classical
3. lectin pathway
introduction of adaptive immunity. classification of adaptive immunity, factor affecting it and mechanism of adaptive immunity comparison between adaptive immunity and innate immunity. characteristic of adaptive immunity . cell mediated immune responses immunoglobulins
types of immunoglobulins. functions of immunoglobulins, hypersensitivity reactions
The complement system is a part of the immune system that helps or complements the ability of antibodies and phagocytic cells to clear pathogens from an organism. It is part of the innate immune system, which is not adaptable and does not change over the course of an individual's lifetime.
consists of three pathways: 1. alternative
2. classical
3. lectin pathway
T-Cell Activation
• Concept of immune response
• T cell-mediated immune response
• B cell-mediated immune response
I. Concept of immune response
• A collective and coordinated response to the introduction of foreign substances in an individual mediated by the cells and molecules in the immune system.
II. T cell-mediated immune response
• Cell-mediated immunity is the arm of the adaptive immune response whose role is to combat infection of intracellular pathogens, such as intracellular bacteria (mycobacteria, listeria monocytogens), viruses, protozoa, etc.
Describes the complement system components and their activation pathways, the regulation of the complement
system, the effector functions of various complement components,
and the consequences of deficiencies in them.
Lymphocyte is a type of white blood cell in the immune system of jawed vertebrate. Lymphocytes include natural killer cells (which function in cell-mediated, cytotoxic innate immunity), T cells (for cell-mediated, cytotoxic adaptive immunity), and B cells (for humoral, antibody-driven adaptive immunity). They are the main type of cell found in lymph, which prompted the name "lymphocyte". Th all myeloid and lymphoid cells develop from one type of stem cell called as Hematopoietic stem cell is a undifferentiated cell give rise to further diffetentiation of all the immune cell as well as blood cells include the T- cell and B-cell. The B-cell is synthesis and matured in the Bone Marrow and T- cell is synthesis in Bone marrow but matured in the thymus. In this topic will be discussed how the B-cell and T-cell are developed
This file contains detail study of the complement system of immunology. This document includes the introduction to complement system, different pathways including classical pathway, alternative pathway and lectin pathway and also the functions of complement system.
T-Cell Activation
• Concept of immune response
• T cell-mediated immune response
• B cell-mediated immune response
I. Concept of immune response
• A collective and coordinated response to the introduction of foreign substances in an individual mediated by the cells and molecules in the immune system.
II. T cell-mediated immune response
• Cell-mediated immunity is the arm of the adaptive immune response whose role is to combat infection of intracellular pathogens, such as intracellular bacteria (mycobacteria, listeria monocytogens), viruses, protozoa, etc.
Describes the complement system components and their activation pathways, the regulation of the complement
system, the effector functions of various complement components,
and the consequences of deficiencies in them.
Lymphocyte is a type of white blood cell in the immune system of jawed vertebrate. Lymphocytes include natural killer cells (which function in cell-mediated, cytotoxic innate immunity), T cells (for cell-mediated, cytotoxic adaptive immunity), and B cells (for humoral, antibody-driven adaptive immunity). They are the main type of cell found in lymph, which prompted the name "lymphocyte". Th all myeloid and lymphoid cells develop from one type of stem cell called as Hematopoietic stem cell is a undifferentiated cell give rise to further diffetentiation of all the immune cell as well as blood cells include the T- cell and B-cell. The B-cell is synthesis and matured in the Bone Marrow and T- cell is synthesis in Bone marrow but matured in the thymus. In this topic will be discussed how the B-cell and T-cell are developed
This file contains detail study of the complement system of immunology. This document includes the introduction to complement system, different pathways including classical pathway, alternative pathway and lectin pathway and also the functions of complement system.
Complement system consists of a group of more then 30 proteins or soluble proteins which are present in the plasma. Basically it is a part of our immune system which enhance the abitlity of Antobody and the Phagocytic cells from the organisms body or also act in inflammation and form the MAC to kill the pathogens.
These are the complements protein or Glycoproteins or Specialized proteins.
Complement System comprises of Complement proteins that function to augment the antibodies in killing bacteria by the formation of Membrane Attack Complex.
This ppt describes the different pathways of activation complement proteins and MAC formation.
Through this presentation you will be able to learn about the detailed knowledge of complement system and its functions along with the complement activation pathways [classical, alternative, lectin pathway ]
This presentation describes the Fish Complement system and different types of pathways involved and the mechanism behind the regulation of complement proteins. It gives a basic and a detailed explanation regarding the topic.
Heavy metal poisoning is caused by the accumulation of certain metals in the body due to exposure through food, water, industrial chemicals, or other sources. While your body needs small amounts of some heavy metals to function normally — such as zinc, copper, chromium, iron, and manganese — toxic amounts are harmful.
Interpretation of dna typing results and codis Neha Agarwal
An STR genotype is the allele, in the case of a homozygote, or alleles, in the
case of a heterozygote, present in a sample for a particular locus and is normally
reported as the number of repeats present in the allele. A full sample genotype
or STR profi le is produced by the combination of all of the locus genotypes into
a single series of numbers. This profi le is what is entered into a case report or
a DNA database for comparison purposes to other samples.
Sample collection and preservation of biological samplesNeha Agarwal
A preliminary survey should be carried out to evaluate potential evidence. In particular, the
recognition of evidence plays a critical role in solving or prosecuting crimes. The priority of the
potential evidence at crime scenes should be assessed based on each item’s relevance to the solution
of the case. Higher priority should be assigned to evidence with probative value to the case.
For example, the evidence related to a corpus delicti is considered to be of the highest priority.
Corpus delicti is a Latin term meaning “body of crime.” In Western law, it primarily refers to the
principle that in order for an individual to be convicted, it is necessary to prove the occurrence of the crime. In a forensic investigation, it also refers to the physical evidence proving that a crime was committed
Scope and significance of forensic chemistryNeha Agarwal
Forensic chemistry is the application of chemistry and its subfield, forensic toxicology, in a legal setting. A forensic chemist can assist in the identification of unknown materials found at a crime scene.[1] Specialists in this field have a wide array of methods and instruments to help identify unknown substances. These include high-performance liquid chromatography, gas chromatography-mass spectrometry, atomic absorption spectroscopy, Fourier transform infrared spectroscopy, and thin layer chromatography. The range of different methods is important due to the destructive nature of some instruments and the number of possible unknown substances that can be found at a scene. Forensic chemists prefer using nondestructive methods first, to preserve evidence and to determine which destructive methods will produce the best results.
Along with other forensic specialists, forensic chemists commonly testify in court as expert witnesses regarding their findings. Forensic chemists follow a set of standards that have been proposed by various agencies and governing bodies, including the Scientific Working Group on the Analysis of Seized Drugs. In addition to the standard operating procedures proposed by the group, specific agencies have their own standards regarding the quality assurance and quality control of their results and their instruments. To ensure the accuracy of what they are reporting, forensic chemists routinely check and verify that their instruments are working correctly and are still able to detect and measure various quantities of different substances.
Protection of critical information infrastructureNeha Agarwal
Information Infrastructure is the term usually used to describe the totality of inter-connected computers and networks, and information flowing through them. Certain parts of this Information Infrastructure, could be dedicated for management / control etc of infrastructure providers’ e.g. Power generation, Gas/oil pipelines, or support our economy or national
fabric e.g. Banking / Telecom etc. The contribution of the services supported
by these infrastructures, and more importantly, the impact of any sudden
failure or outage on our National well being or National Security marks them as being Critical.
By extension, information infrastructure supporting the operations of Critical Infrastructure (CI) marks this as Critical Information infrastructure (CII). These Networks operate/monitor and control important Governmental and Societal functions and services including, but not limited to, Power (Generation/transmission/ distribution etc), Telecommunication (mobile/landline/internet etc), Transportation (Air/land/rail/sea etc), Defence etc. These CII are becoming increasingly dependent on their information infrastructure for information management, communication and control functions.
“Microbial forensics” has been defined as “a scientific discipline dedicated to analyzing evidence
from a bioterrorism act, biocrime, or inadvertent microorganism/toxin release for attribution
purposes” (Budowle et al., 2003). This emerging discipline is still in the early stages of
development and faces substantial scientific challenges to provide a robust suite of technologies
for identifying the source of a biological threat agent and attributing a biothreat act to a particular
person or group. The unlawful use of biological agents poses substantial dangers to individuals,
public health, the environment, the economies of nations, and global peace. It also is likely that
scientific, political, and media-based controversy will surround any investigation of the alleged
use of a biological agent, and can be expected to affect significantly the role that scientific
information or evidence can play. For these reasons, building awareness of and capacity in
microbial forensics can assist in our understanding of what may have occurred during a biothreat
event, and international collaborations that engage the broader scientific and policy-making
communities are likely to strengthen our microbial forensics capabilities. One goal would be to
create a shared technical understanding of the possibilities—and limitations—of the scientific
bases for microbial forensics analysis._ NCBI
Forensic science utilizes scientific principles to support or negate theories surrounding physical evidence found at a crime scene. As such, forensic scientists analyze evidence gathered or received from crime scenes and present their findings based the results of their analyses.
A forensic science job description may appear distinctly different depending on the area of forensic science being practiced. This is because forensic science is a rather broad field and thus encompasses a number of specialties, all of which are rooted in the natural sciences.
The error (or disturbance) of an observed value is the deviation of the observed value from the (unobservable) true value of a quantity of interest (for example, a population mean), and the residual of an observed value is the difference between the observed value and the estimated value of the quantity of interest (for example, a sample mean).
Suppose there is a series of observations from a univariate distribution and we want to estimate the mean of that distribution (the so-called location model). In this case, the errors are the deviations of the observations from the population mean, while the residuals are the deviations of the observations from the sample mean.
A statistical error (or disturbance) is the amount by which an observation differs from its expected value, the latter being based on the whole population from which the statistical unit was chosen randomly. For example, if the mean height in a population of 21-year-old men is 1.75 meters, and one randomly chosen man is 1.80 meters tall, then the "error" is 0.05 meters; if the randomly chosen man is 1.70 meters tall, then the "error" is −0.05 meters. The expected value, being the mean of the entire population, is typically not observable, and hence the statistical error cannot be observed either.
Most bacteria are free-living organisms that grow by increasing
in mass and then divide by binary fission.
Growth and division are controlled by genes, the expression
of which must be regulated appropriately. Genes
whose activity is controlled in response to the needs of a
cell or organism are called regulated genes. All organisms
also have a large number of genes whose products
are essential to the normal functioning of a growing and
dividing cell, no matter what the conditions are. These
genes are always active in growing cells and are known as
constitutive genes or housekeeping genes; examples include
genes that code for the enzymes needed for protein
synthesis and glucose metabolism. Note that all genes are
regulated on some level. If normal cell function is impaired
for some reason, the expression of all genes, including
constitutive genes, is reduced by regulatory
mechanisms. Thus, the distinction between regulated
and constitutive genes is somewhat arbitrary.
It is over 60 years since Hodgkin and
Huxley1 made the first direct recording of
the electrical changes across the neuronal
membrane that mediate the action
potential. Using an electrode placed inside a
squid giant axon they were able to measure a
transmembrane potential of around 260 mV
inside relative to outside, under resting
conditions (this is called the resting membrane
potential). The action potential is a
transient (,1 millisecond) reversal in the
polarity of this transmembrane potential
which then moves from its point of initiation,
down the axon, to the axon terminals. In a
subsequent series of elegant experiments
Hodgkin and Huxley, along with Bernard
Katz, discovered that the action potential
results from transient changes in the permeability
of the axon membrane to sodium (Na+)
and potassium (K+) ions. Importantly, Na+ and
K+ cross the membrane through independent
pathways that open in response to a change
in membrane potential.
As testimony to their pioneering work, the
fundamental mechanisms described by
Hodgkin, Huxley and Katz remain applicable
to all excitable cells today. Indeed, the
predictions they made about the molecular
mechanisms that might underlie the changes
in membrane permeability showed remarkable
foresight. The molecular basis of the action
potential lies in the presence of proteins
called ion channels that form the permeation
pathways across the neuronal membrane.
Although the first electrophysiological
recordings from individual ion channels were
not made until the mid 1970s,2 Hodgkin and
Huxley predicted many of the properties now
known to be key components of their
function: ion selectivity, the electrical basis
of voltage-sensitivity and, importantly, a
mechanism for quickly closing down the
permeability pathways to ensure that the
action potential only moves along the axon in
one direction.
It is over 60 years since Hodgkin and
Huxley1 made the first direct recording of
the electrical changes across the neuronal
membrane that mediate the action
potential. Using an electrode placed inside a
squid giant axon they were able to measure a
transmembrane potential of around 260 mV
inside relative to outside, under resting
conditions (this is called the resting membrane
potential). The action potential is a
transient (,1 millisecond) reversal in the
polarity of this transmembrane potential
which then moves from its point of initiation,
down the axon, to the axon terminals. In a
subsequent series of elegant experiments
Hodgkin and Huxley, along with Bernard
Katz, discovered that the action potential
results from transient changes in the permeability
of the axon membrane to sodium (Na+)
and potassium (K+) ions. Importantly, Na+ and
K+ cross the membrane through independent
pathways that open in response to a change
in membrane potential.
As testimony to their pioneering work, the
fundamental mechanisms described by
Hodgkin, Huxley and Katz remain applicable
to all excitable cells today. Indeed, the
predictions they made about the molecular
mechanisms that might underlie the changes
in membrane permeability showed remarkable
foresight. The molecular basis of the action
potential lies in the presence of proteins
called ion channels that form the permeation
pathways across the neuronal membrane.
Although the first electrophysiological
recordings from individual ion channels were
not made until the mid 1970s,2 Hodgkin and
Huxley predicted many of the properties now
known to be key components of their
function: ion selectivity, the electrical basis
of voltage-sensitivity and, importantly, a
mechanism for quickly closing down the
permeability pathways to ensure that the
action potential only moves along the axon in
one direction.
The term river system refers to a ‘river along with its tributaries’.
Based on their source, the Indian River system is classified in to - Himalayan Rivers and Peninsular Rivers.
The Himalayan Rivers, as the name suggests originate from the Himalayas and flow through the Northern Plains.
The major Himalayan River systems are
The Indus River System,
The Ganga River System
The Yamuna River System
The Brahmaputra River System
Peninsular River System or Peninsular Drainage emerges mainly from the Western Ghats. Since the Western Ghats form a ‘water divide’, these rivers either flow eastwards into the Bay of Bengal or into the Arabian Sea towards the west. Peninsular Rivers are basically ‘rain fed’ rivers.
The major Peninsular River Systems are:
Mahanadi
Godavari
Krishna
Cauvery
Drain into Bay of Bengal as they flow eastwards on the plateau and make ‘deltas’ at their mouths; whereas Narmada
Tapti - the west flowing rivers fall into the Arabian Sea and make ‘estuaries’.
not originate in glaciers, but are rain fed rivers. These rivers reduce considerably or dry up during summers.
Sericulture is the silk producing agro-industry
India is the second largest silk producing country in the world after china.
Sericulture or silk farming is the rearing of silkworm for the production of silk
Silk is known as queen of textile and biosteel because of its strength
A Chinese tale of the discovery of the silkworm’s silk was by an ancient empress Lei Zu , the wife of the emperor.
She was drinking tea under tree, when a silk cocoon fell into her tea cup and the hot tea loosened the long strand of silk
As she it out, and started to wrap the silk thread around her flinger, she felt the warm sensation
When silk ran out, an larva appeared. She realized that it was this larva that produces the silk
Soon, she taught this to people and it became wide spread
Echinococcus granulosus, also called hydatid worm belongs to class Cestoda
It causes cystic echinococcosis in livestock and humans being intermediate hosts and parasitize the small intestines of adult canids
It is a zoonotic disease
Definitive hosts are carnivorous predators like dogs, wolves, foxes and lions. While sheep, goat, cattle, pigs and rodents are intermediate hosts. Birds and arthropods act as mechanical vectors
Krebs cycle or tricarboxylic cycle or citric acid cycleNeha Agarwal
The citric acid cycle is the final common pathway for the oxidation of fuel molecules — amino acids, fatty acids, and carbohydrates.
Hans Adolf Krebs. Biochemist; born in Germany. Worked in Britain. His discovery in 1937 of the ‘Krebs cycle’ of chemical reactions was critical to the understanding of cell metabolism and earned him the 1953 Nobel Prize for Physiology or Medicine.
Induced breeding is a technique where organism is stimulated by particular hormone or other synthetic hormone or by providing condition, introduced to breed in captive condition.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
2. A complex consisting of at least 20 serum proteins,
which, once activated, acts like a part of the innate
immune defense
The complement components are present in serum
in inactive form
The complement is activated in a cascading
manner (= each protein activates that following)
and it has widespread physiologic and
pathophysiologic effects
Complement proteins are synthesized mainly in the
liver, but tissue macrophages and fibroblasts can
synthesize some complement proteins as well
5. (1) The complement pathways are initiated by proteins that bind to
pathogens, either directly or via an antibody or other
pathogenspecifi c protein. After a conformational change,
(2) Enzymatic mediators activate other enzymes that generate the
central proteins of the complement cascade, the C3 and C5
convertases, which cleave C3 and C5, releasing active
components that mediate all functions of complement, including
(3) opsonization,
(4) Inflammation, and
(5) the generation of the membrane attack complex (MAC).
Effector complement proteins can label an antibody-antigen
complex for phagocytosis (opsonins), initiate inflammation
(anaphylatoxins), or bind to a pathogen and nucleate the formation
of the MAC. Often, these eff ectors act through
(6) complement receptors on phagocytic cells, granulocytes, or
erythrocytes.
(7) Regulatory proteins limit the eff ects of complement by
promoting their degradation or preventing their binding to host
Proteins Involved in the Complement System
6.
7. Pathways of complement
activationAlthough the initiating event of each of the three pathways
of complement activation is different, they all converge in
the generation of an enzyme complex capable of cleaving
the C3 molecule into two fragments, C3a and C3b. Th e
enzymes that accomplish this biochemical transformation
are referred to as C3 convertases. The classical and
lectin pathways use the dimer C4b2a for their C3
convertase activity, while the alternative pathway
uses C3bBb to achieve the same end; however, the fi nal
result is the same: a dramatic increase in the
concentration of C3b, a centrally located and
multifunctional complement protein. C3b is an opsonin
The second set of convertase enzymes of the cascade,C5
convertases, are formed by the addition of a C3b
component to the C3 convertases. C5 convertases
cleave C5 into the infl ammatory mediator, C5a, and C5b,
15. C4b2a3b complex, also called C5
convertase, cleaves C5 into C5a, which is a
soluble inflammatory mediator, and C5b,
which is capable of complexing with
additional complement components.
The generation of C5b initiates the final
phase of complement activation, which is
the formation of the Membrane Attack
Complex (MAC). The MAC is identical for
all pathways of complement activation.
C3a and C5a remain soluble and produce
local inflammatory effects.
16.
17.
18. Up to this point in the complement cascades, all of the complement reactions take
place on the hydrophilic surfaces of microbes or on immune complexes in the fl
uid phase of blood, lymph, or tissues. In contrast, when C5b binds C6 and C7,
the resulting complex undergoes a conformational change that exposes
hydrophobic regions on the surface of the C7 component capable of inserting
into the interior of the microbial membrane Released C5b67 complexes can
insert into the membrane of nearby cells and mediate “innocent bystander”
lysis. Under physiologic conditions, such lysis is usually minimized by
regulatory proteins C8 is made up of two peptide chains: C8 alpha gamma and
C8beta. Binding of C8beta to the C5b67 complex induces a conformational
change in the C8 dimer such that the hydrophobic domain of C8 can insert into
the interior of the phospholipid membrane. The C5b678 complex can create a
small pore, 10 Å in diameter, and formation of this pore can lead to lysis of red
blood cells, but not of nucleated cells. The final step in the formation of the
MAC is the binding and polymerization of C9 to the C5b678 complex. As many
as 10 to 19 molecules of C9 can be bound and polymerized by a single
C5b678 complex. During polymerization, the C9 molecules undergo a
transition, so that they, too, can insert into the membrane. The completed
MAC, which has a tubular form and functional pore diameter of 70 Å to 100 Å,
consists of a C5b678 complex surrounded by a poly-C9 complex (Figure 6-
10b). Loss of plasma membrane integrity leads irreversibly to cell death.
19.
20.
21. Human Complement
Component Deficiencies
Deficiencies of components.
Deficiencies of complement
components are very rare.
Defects in the early components of the
classical pathway do not lead to
overwhelming infection, as the MBL
and alternative pathways can bypass
this defect
