2. Electrophoresis Technique General Use
Routine electrophoresis Mostly used for separation of proteins and has some use in separating nucleic acids(1)
High resolution electrophoresis High resolution electrophoresis is used for detecting monoclonal immunoglobulins in serum and urine. Proteins with similar electrophoretic
mobilities and those present in low concentrations and that may be invisible on other methods can be detected and separated by this method
due to its superior resolution and sensitivity. (2)
Polyacrylamide gel electrophoresis (PAGE) PAGE is used to study individual proteins. It can separate, identify, and quantify them in certain cells and environments. (4) It can separate
serum proteins into 20 or more fractions than the usual 5 fractions separated by cellulose or agarose. (5)
Capillary electrophoresis This method is used for the separation, quantitation, and determination of molecular weights of proteins and peptides. It is also used for the
analysis of products from polymerase chain reaction (PCR) and for the analysis of inorganic ions, organic acids, pharmaceuticals, optical
isomers, and drugs of abuse in serum and urine. (5)
Isoelectric focusing A technique of exquisite resolution and high sensitivity that enables greater discrimination between the various immunoglobulin abnormalities
encountered in the clinical laboratory.(7)
Two-dimensional electrophoresis Combines two different electrophoresis dimensions to separate proteins from complex matrices such as serum or tissue.(5)
Pulsed field electrophoresis Used to produce DNA fingerprints for a bacterial isolate. This technique investigates the bacterial isolates from sick people, contaminated food,
and the places where food is produced. (8)
