Temozolomide And Doxorubicin Delivery
•Download as PPT, PDF•
1 like•509 views
1) Chitosan was grafted with polylactide of different molecular weights, 10 kDa and 60 kDa, to form nanoparticles for co-delivery of doxorubicin and temozolomide. 2) The nanoparticles were characterized and found to have sizes between 150-350 nm and zeta potentials of 25-45 mV, indicating stability. They showed high encapsulation efficiency of the drugs at both acidic and neutral pH. 3) In vitro drug release studies demonstrated sustained release of both drugs over 2 weeks from the nanoparticles, with the rate of release increasing at higher pH. The nanoparticles based on lower molecular weight polylactide released temozolomide faster than doxorubic
Report
Share
Report
Share
Recommended
Identification of side products from the hydrogenation reaction of bis(substi...
The document describes a hydrogenation reaction of bis(substitutedbenzylidene)cyclopentanone using palladium on carbon catalyst. This reaction produced the expected tetrahydro products in moderate yields. Additionally, it produced three side products that were characterized as alkane and alcohol forms of the cyclopentane ring. The structures of the side products were determined using spectroscopic methods such as IR, NMR, and MS.
Validation of Factor II and Xa Chromogenic Assays for Dalteparin, Enoxaparin,...
A chromogenic assay intended for the quantitative determination in purified solutions by measurement of factor IIa or Xa inhibition activity. The kit can be used for 100 test reactions as per microtiter plate protocol.
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidr...
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidr...Tomsk Polytechnic University
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidrugs controlled and sustained releaseElectrophoresis
This document provides an overview of electrophoresis techniques. It discusses different types of electrophoresis including gel electrophoresis techniques like agarose gel electrophoresis and SDS-PAGE. It explains how agarose and polyacrylamide gels are prepared and the buffers used. It also covers applications of electrophoresis like analyzing DNA, RNA, and proteins, and describes techniques like native PAGE and gradient gel electrophoresis.
Polymerize chain reaction
The document discusses the history and development of the polymerase chain reaction (PCR) technique, beginning with the discovery of DNA replication using primers by Khorana in 1970 and the refinement of the technique by Kary Mullis in 1983 which allowed for exponential amplification of specific DNA regions, and it provides details on the components and steps of a basic PCR reaction including primers, DNA polymerase, nucleotides, and cycling conditions.
Validation of factor xa assay for dalteparin sodium dalteparin injection
chromogenic assay intended for the quantitative determination in purified solutions by measurement of factor IIa or Xa inhibition activity. The kit can be used for 100 test reactions as per microtiter plate protocol.
Residual Solvent Analysis; part 1 GC-Head Space Calculations of Residual Solv...
Residual Solvent Analysis; part 1 GC-Head Space Calculations of Residual Solv...Alba Casta Pharma Solutions, India
GC Head space Analysis, Residual Solvents by GC-HS, ICH Guidelines, ICH Q3C (R6), Pharmaceutical impurities, Impurities in pharmaceuticals, Drug substances, drug products, drug excipient, Residual solvent analysis.
Solvents are critical inputs in synthetic processes. An appropriate solvent for synthesis of drug substance may enhance the yield or determine characteristics such as polymorph, purity, solubility and bio-availability. After the desired form of drug substance is achieved, solvents are no longer needed as they do not provide any therapeutic benefit. All residual solvents should, ideally be removed completely to meet product specifications, good manufacturing practices, and other quality-based requirements. However, manufacturing processes cannot remove the solvents completely from drug substances and products and some residue of them are left in pharmaceuticals.
The precise amount of a solvent can be measured in direct injection method, based on its concentration and volume of the injection. However, in GC headspace this is not possible since saturated vapors of solvents, accumulated over headspace, are fed into GC system. Responses of respective solvents depend on their relative vapor pressure in diluent which in turn depends on the nature and volume of the diluent. Therefore, weight calculation of impurity or solvent in GC headspace analyses is not straightforward, the topic of this lecture.
https://youtu.be/uViLLtaOlnkValidation of Factor IIa assay for nadroparin calcium or nadroparin injection
A chromogenic assay intended for the quantitative determination in purified solutions by measurement of factor IIa or Xa inhibition activity. The kit can be used for 100 test reactions as per microtiter plate protocol.
Recommended
Identification of side products from the hydrogenation reaction of bis(substi...
The document describes a hydrogenation reaction of bis(substitutedbenzylidene)cyclopentanone using palladium on carbon catalyst. This reaction produced the expected tetrahydro products in moderate yields. Additionally, it produced three side products that were characterized as alkane and alcohol forms of the cyclopentane ring. The structures of the side products were determined using spectroscopic methods such as IR, NMR, and MS.
Validation of Factor II and Xa Chromogenic Assays for Dalteparin, Enoxaparin,...
A chromogenic assay intended for the quantitative determination in purified solutions by measurement of factor IIa or Xa inhibition activity. The kit can be used for 100 test reactions as per microtiter plate protocol.
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidr...
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidr...Tomsk Polytechnic University
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidrugs controlled and sustained releaseElectrophoresis
This document provides an overview of electrophoresis techniques. It discusses different types of electrophoresis including gel electrophoresis techniques like agarose gel electrophoresis and SDS-PAGE. It explains how agarose and polyacrylamide gels are prepared and the buffers used. It also covers applications of electrophoresis like analyzing DNA, RNA, and proteins, and describes techniques like native PAGE and gradient gel electrophoresis.
Polymerize chain reaction
The document discusses the history and development of the polymerase chain reaction (PCR) technique, beginning with the discovery of DNA replication using primers by Khorana in 1970 and the refinement of the technique by Kary Mullis in 1983 which allowed for exponential amplification of specific DNA regions, and it provides details on the components and steps of a basic PCR reaction including primers, DNA polymerase, nucleotides, and cycling conditions.
Validation of factor xa assay for dalteparin sodium dalteparin injection
chromogenic assay intended for the quantitative determination in purified solutions by measurement of factor IIa or Xa inhibition activity. The kit can be used for 100 test reactions as per microtiter plate protocol.
Residual Solvent Analysis; part 1 GC-Head Space Calculations of Residual Solv...
Residual Solvent Analysis; part 1 GC-Head Space Calculations of Residual Solv...Alba Casta Pharma Solutions, India
GC Head space Analysis, Residual Solvents by GC-HS, ICH Guidelines, ICH Q3C (R6), Pharmaceutical impurities, Impurities in pharmaceuticals, Drug substances, drug products, drug excipient, Residual solvent analysis.
Solvents are critical inputs in synthetic processes. An appropriate solvent for synthesis of drug substance may enhance the yield or determine characteristics such as polymorph, purity, solubility and bio-availability. After the desired form of drug substance is achieved, solvents are no longer needed as they do not provide any therapeutic benefit. All residual solvents should, ideally be removed completely to meet product specifications, good manufacturing practices, and other quality-based requirements. However, manufacturing processes cannot remove the solvents completely from drug substances and products and some residue of them are left in pharmaceuticals.
The precise amount of a solvent can be measured in direct injection method, based on its concentration and volume of the injection. However, in GC headspace this is not possible since saturated vapors of solvents, accumulated over headspace, are fed into GC system. Responses of respective solvents depend on their relative vapor pressure in diluent which in turn depends on the nature and volume of the diluent. Therefore, weight calculation of impurity or solvent in GC headspace analyses is not straightforward, the topic of this lecture.
https://youtu.be/uViLLtaOlnkValidation of Factor IIa assay for nadroparin calcium or nadroparin injection
A chromogenic assay intended for the quantitative determination in purified solutions by measurement of factor IIa or Xa inhibition activity. The kit can be used for 100 test reactions as per microtiter plate protocol.
MECC 2016 Poster
This study evaluated substituted anthraquinones as inhibitors of DNA methyltransferase 1 (DNMT1). An endonuclease-coupled assay was used to screen 15 simplified anthraquinone compounds for their ability to inhibit DNMT1. One compound, compound 13, successfully inhibited DNMT1 activity in secondary assays and was determined to be a direct DNMT1 inhibitor. Compound 13 competitively inhibited DNMT1 binding to DNA with a Ki of 13 μM and could still inhibit DNMT1 activity even when an autoinhibitory domain was present. While less potent than the natural product laccaic acid A, compound 13 has a less complex structure and represents a potential new scaffold for developing DNMT1 inhibitors.
Physical, Thermal and Structural Properties of Chromium (vi) Oxide Powder:Imp...
Physical, Thermal and Structural Properties of Chromium (vi) Oxide Powder:Imp...Mahendra Kumar Trivedi
The objective of the present study was to analyse the alterations in physical, thermal and structural properties of Chromium VI Oxide powder under the influence of Mahendra Trivedi's biofield energy treatment. In this study, the FTIR, XRD & TGA-DTA analysis of CrO3 was conducted to evaluate changes in its characteristics.Validation of factor xa assay for nadroparin calcium nadroparin injection
This document describes a chromogenic assay used to quantify the anticoagulant Nadroparin in samples by measuring its inhibitory effect on factor Xa. The assay involves incubating Nadroparin samples with factor Xa and anti-thrombin III, then adding a chromogenic substrate and measuring the absorbance. The absorbance is inversely proportional to the Nadroparin concentration as it inhibits factor Xa activity. The assay kit and procedure are validated for use in quantifying Nadroparin levels in bioequivalence studies.
Validation of factor i ia assay for tinzaparin sodium-tinzaparin injection
chromogenic assay intended for the quantitative determination in purified solutions by measurement of factor IIa or Xa inhibition activity. The kit can be used for 100 test reactions as per microtiter plate protocol.
Validation of factor xa assay for tinzaparin sodium tinzaparin injection
chromogenic assay intended for the quantitative determination in purified solutions by measurement of factor IIa or Xa inhibition activity. The kit can be used for 100 test reactions as per microtiter plate protocol.
Eckenhoff et al 2001
This study investigated the binding targets of the inhaled anesthetic halothane in rat brain. The researchers used quantitative photoaffinity labeling and electrophoresis to analyze rat cerebellar homogenates exposed to [14C]halothane. They found that halothane incorporated into many soluble and membrane-bound proteins, with stoichiometry values ranging from 0 to 4 and apparent IC50 values from 0.2 to 2.0 mM. Competition experiments showed that unlabeled halothane, chloroform, and isoflurane inhibited halothane labeling to varying degrees, while a non-anesthetic compound inhibited the least. These results suggest that halothane binding motifs can be found in a wide variety of proteins in the brain
Sterilization mathematics (F0. Fphy, Fbio, Sterility Assurance (SAL) calculat...
Sterile means free from viable microorganisms and sterilization is any physical or chemical process which destroys all life forms, with special regard to microorganisms (including bacteria and sporogenous forms) and inactivates viruses.
Therefore, the terms “sterile” and “sterilization”, in a strictly biological sense, describe the absence and, respectively, the destruction of all viable microorganisms. In other words, they are absolute terms: an object or system is either “sterile” or “non-sterile”.
The destruction of a microbial population subjected to a sterilization process follows a logarithmic progression: only a treatment of infinite duration can provide the absolute certainty that the entire microbial population has been destroyed, and that the system is sterile.
thesis presentation
1) The document summarizes research on fabricating a Bismuth-Chitosan modified glassy carbon electrode and applying it to measure Tartrazine levels in food and drug samples.
2) Key aspects of the electrode modification and optimization process are described, including ex-situ plating of Bismuth and use of Chitosan.
3) The modified electrode showed good electrocatalytic activity for Tartrazine and was used to accurately determine Tartrazine concentrations in bisacodyl tablets and Eminem candy samples.
Nanotechnology and potential in Cancer therapy and treatment
this presentation focuses on new nanotechnology and it possible use in detection and therapy with cancer. it was prepared by final year biochemistry student at NCU.
Nanotechnology in treatment of cancer
This document discusses the potential applications of nanotechnology in cancer diagnosis and treatment. It begins with an overview of nanotechnology and nanomedicine. It then discusses how cancer forms and the various factors that can cause cancer like chemicals, radiation, viruses and lifestyle. The document outlines how nanotechnology can be used to more effectively deliver drugs, detect cancer at an early stage, and treat cancer through approaches like photothermal ablation using gold nanoparticles. It acknowledges challenges like ensuring nanoparticles are biocompatible and non-toxic, but envisions that human clinical trials within the next few years could demonstrate how nanotechnology allows for safer and more targeted cancer treatment.
Nanotechnology in cancer therapy
Cancer is one of the leading causes of death worldwide and is characterized by uncontrolled cell growth. Current cancer therapies like surgery, radiotherapy, and chemotherapy can be highly efficient but have low selectivity, therapeutic index, and cause side effects. Nanotechnology and nanomedicine, which involve manipulating matter at the atomic and molecular scale, show promise for more targeted cancer therapy by allowing drugs to be encapsulated in nanoparticles and delivered specifically to tumor sites, potentially reducing side effects and improving treatment outcomes. In particular, gold nanoparticles show potential for photothermal therapy, radiotherapy, and inhibiting angiogenesis in cancer treatment due to their tunable properties and ability to accumulate in tumors.
Amphiphilic polymeric nanoparticles for drug delivery
This document discusses stimuli-responsive polymers (SRP), specifically those responsive to pH and temperature. It summarizes various pH- and temperature-responsive polymers used in biomedical applications like drug delivery. It then describes the synthesis of an amphiphilic co-polymer called CS-g-PLACA by grafting carboxyl-functionalized polylactide to chitosan. Polymeric nanoparticles were formed from CS-g-PLACA using a solvent-free method and were found to be temperature responsive, swelling and collapsing depending on temperature and pH. The nanoparticles have potential applications as responsive drug delivery systems and in gene therapy.
Research
1) The document discusses research on polymer gels, nanogels, and their applications in drug delivery.
2) Simulation methods and theoretical models are used to study the phase behavior, swelling properties, and self-assembly of polyelectrolyte gels and nanogels under different conditions.
3) Ongoing work includes atomistic simulations of polymer-drug interactions to inform drug delivery applications of gels and nanogels.
Polymeric nanoparticles for encapsulation and controlled release of bioactive...
Polymeric nanoparticles for encapsulation and controlled release of bioactive...Tomsk Polytechnic University
This document discusses polymeric nanoparticles for encapsulation and controlled release of bioactive compounds. Section 1 discusses polysaccharide-based nanocomplexes of chitosan and alginic acid for co-encapsulation and sustained release of 5-fluorouracil and temozolomide. The nanoparticles had sizes around 100-200 nm, encapsulation efficiencies between 20-80%, and provided sustained release over one week. Section 2 explores chitosan grafted with low molecular weight polylactic acid for protein encapsulation and reducing initial burst release. The grafted polylactic acid prolonged release in acidic media and reduced initial burst. Section 3 examines amphiphilic nanoparticles using chitosan grafted with polylNANOTECHNOLOGY FOR CANCER THERAPY RECENT DEVELOPMENTS
This document discusses the use of nanotechnology for cancer therapy and recent developments. It describes how nanotechnology platforms can serve as targeted drug delivery vehicles, carrying therapeutic agents into malignant cells while avoiding healthy cells. The document then discusses several nanotechnology approaches for cancer treatment, including using nanoparticles loaded with anticancer drugs for targeted delivery and preventing DNA damage through coating skin cells with sunscreen-containing nanoparticles on the nanoscale. Overall, the document outlines the potential of nanotechnology to improve cancer treatment by more precisely targeting cancer cells and reducing side effects.
Nanoparticulate drug delivery sysytem
Nanoparticulate drug delivery systems can provide several advantages over traditional medications. Nanoparticles are sub-100nm structures that can encapsulate drugs and biologically active substances. They can improve drug efficacy, reduce toxicity, enhance distribution in the body, and improve patient compliance. Common types of nanoparticles used for drug delivery include polymeric nanoparticles, solid lipid nanoparticles, nanosuspensions, liposomes, dendrimers, and magnetic nanoparticles. Nanoparticles are prepared using various methods such as cross-linking of amphiphilic polymers, emulsion polymerization, and precipitation of hydrophobic polymers from organic solvents. The small size of nanoparticles allows for targeted drug delivery to specific sites in the body.
Preparation methods of polymeric nanoparticles
This document discusses methods for preparing polymeric nanoparticles. There are two main types of polymers used: natural hydrophilic polymers like proteins and polysaccharides, and synthetic lipophilic polymers that are either pre-polymerized or polymerized during preparation. The main preparation methods are amphiphilic macromolecule cross-linking using heat or chemical cross-linkers, polymerization-based methods involving emulsion, dispersion, or interfacial condensation polymerization, and polymer precipitation methods using solvent extraction/evaporation, solvent displacement, or salting out. Common techniques include single or double emulsion followed by solvent evaporation to create drug-loaded nanoparticles.
Nanotechnology Based Drug Delivery11
The emergence of nanotechnology is likely to have a significant impact on drug delivery sector, affecting just about every route of administration from oral to injectable, according to specialist market research firm NanoMarkets.
Polysaccharides based nanoparticles for drug delivery application
This document discusses the use of polysaccharide-based nanoparticles for drug delivery applications. It describes how nanoparticles between 10-700nm in size can encapsulate and provide controlled release of drugs while decreasing side effects. Polysaccharides are promising materials for nanoparticles due to their biocompatibility, low cost, and reactive groups. The document outlines methods for preparing nanoparticles from chitosan, alginate, pectin, and other polysaccharides using techniques like ionic crosslinking. Characterization of the nanoparticles in solution and solid form is presented, showing properties like particle size and surface charge. Future work aims to use the nanoparticles to encapsulate drugs and study their release, uptake, and shape evolution in physiological conditions.
Novel PLA chitosan nanocomplexes for controlled release of DNA alkylating drugs
Novel PLA chitosan nanocomplexes for controlled release of DNA alkylating drugsTomsk Polytechnic University
This document discusses the development of novel PLA-chitosan nanocomplexes for controlled drug delivery. Key points:
- Nanocomplexes were prepared from chitosan and different types of polylactic acid (PLA) to encapsulate DNA alkylating drugs like doxorubicin, temozolomide, and 5-fluorouracil.
- The PLA side chain structure influenced nanoparticle size and properties - branched PLA formulations showed higher drug encapsulation efficiency and reduced initial burst release compared to linear or carboxylated PLA.
- Drugs were released in a sustained manner from the nanocomplexes over several days. Loading the drugs simultaneously did notNanoemulsions
Nanoemulsions are dispersions of two immiscible liquids stabilized by a surfactant, with droplet sizes ranging from 5-200nm. They are thermodynamically or kinetically stable. Nanoemulsions can be classified as oil-in-water, water-in-oil, or bi-continuous depending on their composition. They are prepared using low energy methods like phase inversion temperature or high energy methods using mechanical devices. Nanoemulsions show potential for drug delivery by increasing drug loading and bioavailability while providing controlled release and protecting drugs.
Biological transport of nanoparticles
The document discusses the use of nanoparticles for biological transport and drug delivery in the human body. It explains that nanoparticles are well-suited for drug delivery due to their small size which allows them to access cells and organelles through blood capillaries and pores. The document also reviews how nanoparticles can be transported through the body via blood vessels and cross cellular barriers either transcellularly or paracellularly to reach their target sites.
More Related Content
What's hot
MECC 2016 Poster
This study evaluated substituted anthraquinones as inhibitors of DNA methyltransferase 1 (DNMT1). An endonuclease-coupled assay was used to screen 15 simplified anthraquinone compounds for their ability to inhibit DNMT1. One compound, compound 13, successfully inhibited DNMT1 activity in secondary assays and was determined to be a direct DNMT1 inhibitor. Compound 13 competitively inhibited DNMT1 binding to DNA with a Ki of 13 μM and could still inhibit DNMT1 activity even when an autoinhibitory domain was present. While less potent than the natural product laccaic acid A, compound 13 has a less complex structure and represents a potential new scaffold for developing DNMT1 inhibitors.
Physical, Thermal and Structural Properties of Chromium (vi) Oxide Powder:Imp...
Physical, Thermal and Structural Properties of Chromium (vi) Oxide Powder:Imp...Mahendra Kumar Trivedi
The objective of the present study was to analyse the alterations in physical, thermal and structural properties of Chromium VI Oxide powder under the influence of Mahendra Trivedi's biofield energy treatment. In this study, the FTIR, XRD & TGA-DTA analysis of CrO3 was conducted to evaluate changes in its characteristics.Validation of factor xa assay for nadroparin calcium nadroparin injection
This document describes a chromogenic assay used to quantify the anticoagulant Nadroparin in samples by measuring its inhibitory effect on factor Xa. The assay involves incubating Nadroparin samples with factor Xa and anti-thrombin III, then adding a chromogenic substrate and measuring the absorbance. The absorbance is inversely proportional to the Nadroparin concentration as it inhibits factor Xa activity. The assay kit and procedure are validated for use in quantifying Nadroparin levels in bioequivalence studies.
Validation of factor i ia assay for tinzaparin sodium-tinzaparin injection
chromogenic assay intended for the quantitative determination in purified solutions by measurement of factor IIa or Xa inhibition activity. The kit can be used for 100 test reactions as per microtiter plate protocol.
Validation of factor xa assay for tinzaparin sodium tinzaparin injection
chromogenic assay intended for the quantitative determination in purified solutions by measurement of factor IIa or Xa inhibition activity. The kit can be used for 100 test reactions as per microtiter plate protocol.
Eckenhoff et al 2001
This study investigated the binding targets of the inhaled anesthetic halothane in rat brain. The researchers used quantitative photoaffinity labeling and electrophoresis to analyze rat cerebellar homogenates exposed to [14C]halothane. They found that halothane incorporated into many soluble and membrane-bound proteins, with stoichiometry values ranging from 0 to 4 and apparent IC50 values from 0.2 to 2.0 mM. Competition experiments showed that unlabeled halothane, chloroform, and isoflurane inhibited halothane labeling to varying degrees, while a non-anesthetic compound inhibited the least. These results suggest that halothane binding motifs can be found in a wide variety of proteins in the brain
Sterilization mathematics (F0. Fphy, Fbio, Sterility Assurance (SAL) calculat...
Sterile means free from viable microorganisms and sterilization is any physical or chemical process which destroys all life forms, with special regard to microorganisms (including bacteria and sporogenous forms) and inactivates viruses.
Therefore, the terms “sterile” and “sterilization”, in a strictly biological sense, describe the absence and, respectively, the destruction of all viable microorganisms. In other words, they are absolute terms: an object or system is either “sterile” or “non-sterile”.
The destruction of a microbial population subjected to a sterilization process follows a logarithmic progression: only a treatment of infinite duration can provide the absolute certainty that the entire microbial population has been destroyed, and that the system is sterile.
thesis presentation
1) The document summarizes research on fabricating a Bismuth-Chitosan modified glassy carbon electrode and applying it to measure Tartrazine levels in food and drug samples.
2) Key aspects of the electrode modification and optimization process are described, including ex-situ plating of Bismuth and use of Chitosan.
3) The modified electrode showed good electrocatalytic activity for Tartrazine and was used to accurately determine Tartrazine concentrations in bisacodyl tablets and Eminem candy samples.
What's hot (8)
Physical, Thermal and Structural Properties of Chromium (vi) Oxide Powder:Imp...
Physical, Thermal and Structural Properties of Chromium (vi) Oxide Powder:Imp...
Validation of factor xa assay for nadroparin calcium nadroparin injection
Validation of factor xa assay for nadroparin calcium nadroparin injection
Validation of factor i ia assay for tinzaparin sodium-tinzaparin injection
Validation of factor i ia assay for tinzaparin sodium-tinzaparin injection
Validation of factor xa assay for tinzaparin sodium tinzaparin injection
Validation of factor xa assay for tinzaparin sodium tinzaparin injection
Sterilization mathematics (F0. Fphy, Fbio, Sterility Assurance (SAL) calculat...
Sterilization mathematics (F0. Fphy, Fbio, Sterility Assurance (SAL) calculat...
Viewers also liked
Nanotechnology and potential in Cancer therapy and treatment
this presentation focuses on new nanotechnology and it possible use in detection and therapy with cancer. it was prepared by final year biochemistry student at NCU.
Nanotechnology in treatment of cancer
This document discusses the potential applications of nanotechnology in cancer diagnosis and treatment. It begins with an overview of nanotechnology and nanomedicine. It then discusses how cancer forms and the various factors that can cause cancer like chemicals, radiation, viruses and lifestyle. The document outlines how nanotechnology can be used to more effectively deliver drugs, detect cancer at an early stage, and treat cancer through approaches like photothermal ablation using gold nanoparticles. It acknowledges challenges like ensuring nanoparticles are biocompatible and non-toxic, but envisions that human clinical trials within the next few years could demonstrate how nanotechnology allows for safer and more targeted cancer treatment.
Nanotechnology in cancer therapy
Cancer is one of the leading causes of death worldwide and is characterized by uncontrolled cell growth. Current cancer therapies like surgery, radiotherapy, and chemotherapy can be highly efficient but have low selectivity, therapeutic index, and cause side effects. Nanotechnology and nanomedicine, which involve manipulating matter at the atomic and molecular scale, show promise for more targeted cancer therapy by allowing drugs to be encapsulated in nanoparticles and delivered specifically to tumor sites, potentially reducing side effects and improving treatment outcomes. In particular, gold nanoparticles show potential for photothermal therapy, radiotherapy, and inhibiting angiogenesis in cancer treatment due to their tunable properties and ability to accumulate in tumors.
Amphiphilic polymeric nanoparticles for drug delivery
This document discusses stimuli-responsive polymers (SRP), specifically those responsive to pH and temperature. It summarizes various pH- and temperature-responsive polymers used in biomedical applications like drug delivery. It then describes the synthesis of an amphiphilic co-polymer called CS-g-PLACA by grafting carboxyl-functionalized polylactide to chitosan. Polymeric nanoparticles were formed from CS-g-PLACA using a solvent-free method and were found to be temperature responsive, swelling and collapsing depending on temperature and pH. The nanoparticles have potential applications as responsive drug delivery systems and in gene therapy.
Research
1) The document discusses research on polymer gels, nanogels, and their applications in drug delivery.
2) Simulation methods and theoretical models are used to study the phase behavior, swelling properties, and self-assembly of polyelectrolyte gels and nanogels under different conditions.
3) Ongoing work includes atomistic simulations of polymer-drug interactions to inform drug delivery applications of gels and nanogels.
Polymeric nanoparticles for encapsulation and controlled release of bioactive...
Polymeric nanoparticles for encapsulation and controlled release of bioactive...Tomsk Polytechnic University
This document discusses polymeric nanoparticles for encapsulation and controlled release of bioactive compounds. Section 1 discusses polysaccharide-based nanocomplexes of chitosan and alginic acid for co-encapsulation and sustained release of 5-fluorouracil and temozolomide. The nanoparticles had sizes around 100-200 nm, encapsulation efficiencies between 20-80%, and provided sustained release over one week. Section 2 explores chitosan grafted with low molecular weight polylactic acid for protein encapsulation and reducing initial burst release. The grafted polylactic acid prolonged release in acidic media and reduced initial burst. Section 3 examines amphiphilic nanoparticles using chitosan grafted with polylNANOTECHNOLOGY FOR CANCER THERAPY RECENT DEVELOPMENTS
This document discusses the use of nanotechnology for cancer therapy and recent developments. It describes how nanotechnology platforms can serve as targeted drug delivery vehicles, carrying therapeutic agents into malignant cells while avoiding healthy cells. The document then discusses several nanotechnology approaches for cancer treatment, including using nanoparticles loaded with anticancer drugs for targeted delivery and preventing DNA damage through coating skin cells with sunscreen-containing nanoparticles on the nanoscale. Overall, the document outlines the potential of nanotechnology to improve cancer treatment by more precisely targeting cancer cells and reducing side effects.
Nanoparticulate drug delivery sysytem
Nanoparticulate drug delivery systems can provide several advantages over traditional medications. Nanoparticles are sub-100nm structures that can encapsulate drugs and biologically active substances. They can improve drug efficacy, reduce toxicity, enhance distribution in the body, and improve patient compliance. Common types of nanoparticles used for drug delivery include polymeric nanoparticles, solid lipid nanoparticles, nanosuspensions, liposomes, dendrimers, and magnetic nanoparticles. Nanoparticles are prepared using various methods such as cross-linking of amphiphilic polymers, emulsion polymerization, and precipitation of hydrophobic polymers from organic solvents. The small size of nanoparticles allows for targeted drug delivery to specific sites in the body.
Preparation methods of polymeric nanoparticles
This document discusses methods for preparing polymeric nanoparticles. There are two main types of polymers used: natural hydrophilic polymers like proteins and polysaccharides, and synthetic lipophilic polymers that are either pre-polymerized or polymerized during preparation. The main preparation methods are amphiphilic macromolecule cross-linking using heat or chemical cross-linkers, polymerization-based methods involving emulsion, dispersion, or interfacial condensation polymerization, and polymer precipitation methods using solvent extraction/evaporation, solvent displacement, or salting out. Common techniques include single or double emulsion followed by solvent evaporation to create drug-loaded nanoparticles.
Nanotechnology Based Drug Delivery11
The emergence of nanotechnology is likely to have a significant impact on drug delivery sector, affecting just about every route of administration from oral to injectable, according to specialist market research firm NanoMarkets.
Polysaccharides based nanoparticles for drug delivery application
This document discusses the use of polysaccharide-based nanoparticles for drug delivery applications. It describes how nanoparticles between 10-700nm in size can encapsulate and provide controlled release of drugs while decreasing side effects. Polysaccharides are promising materials for nanoparticles due to their biocompatibility, low cost, and reactive groups. The document outlines methods for preparing nanoparticles from chitosan, alginate, pectin, and other polysaccharides using techniques like ionic crosslinking. Characterization of the nanoparticles in solution and solid form is presented, showing properties like particle size and surface charge. Future work aims to use the nanoparticles to encapsulate drugs and study their release, uptake, and shape evolution in physiological conditions.
Novel PLA chitosan nanocomplexes for controlled release of DNA alkylating drugs
Novel PLA chitosan nanocomplexes for controlled release of DNA alkylating drugsTomsk Polytechnic University
This document discusses the development of novel PLA-chitosan nanocomplexes for controlled drug delivery. Key points:
- Nanocomplexes were prepared from chitosan and different types of polylactic acid (PLA) to encapsulate DNA alkylating drugs like doxorubicin, temozolomide, and 5-fluorouracil.
- The PLA side chain structure influenced nanoparticle size and properties - branched PLA formulations showed higher drug encapsulation efficiency and reduced initial burst release compared to linear or carboxylated PLA.
- Drugs were released in a sustained manner from the nanocomplexes over several days. Loading the drugs simultaneously did notNanoemulsions
Nanoemulsions are dispersions of two immiscible liquids stabilized by a surfactant, with droplet sizes ranging from 5-200nm. They are thermodynamically or kinetically stable. Nanoemulsions can be classified as oil-in-water, water-in-oil, or bi-continuous depending on their composition. They are prepared using low energy methods like phase inversion temperature or high energy methods using mechanical devices. Nanoemulsions show potential for drug delivery by increasing drug loading and bioavailability while providing controlled release and protecting drugs.
Biological transport of nanoparticles
The document discusses the use of nanoparticles for biological transport and drug delivery in the human body. It explains that nanoparticles are well-suited for drug delivery due to their small size which allows them to access cells and organelles through blood capillaries and pores. The document also reviews how nanoparticles can be transported through the body via blood vessels and cross cellular barriers either transcellularly or paracellularly to reach their target sites.
are nanostructured lipid carrier are better then solid lipid nanoparticles
The document compares solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) for delivering the drug clotrimazole. SLNs have limitations like drug expulsion during storage due to crystallization. NLCs were developed to overcome these limitations by using mixtures of solid and liquid lipids that create a more imperfect crystal lattice. The study formulated clotrimazole-loaded SLNs and NLCs using emulsification followed by ultrasonication. Particle size, polydispersity index, zeta potential, drug loading, encapsulation efficiency, release, and stability were evaluated and compared for the SLNs and NLCs. NLCs showed higher drug loading and more sustained release compared to SLNs
NANOPARTICULATE DRUG DELIVERY SYSTEM
NANOTECHNOLOGY comprises technological developments on the nanometer scale, usually 0.1 to 100 nm. Nanotechnology, the science of the small. Nano is Greek for dwarf, and nanoscience deals with the study of molecular and atomic particles.
Nano Based Polymers and Applications in Drug Delivery
Nano based polymers have applications in drug delivery. Nanoparticles can be covered by DNA or other polymers to create a "nano-vector" for targeted drug delivery. The nano-vector seeks out specific receptors on tumor cells to deliver its drug payload directly into cancer cells. This allows the drug to potentially destroy tumors without surgery by inhibiting tumor cell cycling and protein synthesis. The nano-vector aims to break down the tumor from within by a targeted, controlled release of drugs directly into cancer cells.
Nanogel drug delivery system
This document provides an overview of nanogels including their classification, properties, synthesis methods, and applications. It discusses how nanogels are nanosized hydrogel particles formed by crosslinking hydrophilic polymers. They can be stimuli-responsive or non-responsive. Methods for synthesizing nanogels include photolithography, membrane emulsification, chemical crosslinking, and polymerization. Nanogels show potential for drug and gene delivery in applications such as cancer treatment, wound healing, and more due to their biocompatibility and ability to encapsulate and release therapeutic agents.
Solid lipid nanopaticle as promising drug
1) Solid lipid nanoparticles (SLNs) are submicron colloidal carriers that are made up of physiological lipids dispersed in water or aqueous surfactant solutions. They provide advantages over traditional liposomes such as good biocompatibility and lower toxicity.
2) SLNs can be prepared using various methods such as high pressure homogenization and ultrasonication. They allow for controlled drug release and encapsulation of both hydrophilic and lipophilic drugs.
3) SLNs have a variety of applications including cancer chemotherapy, brain drug delivery, and dermatological products. They are being researched as carriers for vaccines, anti-parasitic drugs, and tuberculosis treatments.
Nanostructured lipid carriers (NLC)
Nanostructured lipid carriers (NLCs) were presented as a topical drug delivery system. NLCs consist of a blend of solid and liquid lipids which can incorporate drugs at high loading capacities. They were summarized to have advantages over solid lipid nanoparticles including avoidance of drug expulsion and unpredictable gelation. Methods for producing NLCs like high pressure homogenization were described. NLCs were said to increase skin permeation of drugs while providing occlusive and moisturizing properties beneficial for skin care. Several drug-loaded NLC formulations were presented including ones for flurbiprofen, minoxidil, and tacrolimus to improve their topical delivery and stability.
Viewers also liked (20)
Nanotechnology and potential in Cancer therapy and treatment
Nanotechnology and potential in Cancer therapy and treatment
Amphiphilic polymeric nanoparticles for drug delivery
Amphiphilic polymeric nanoparticles for drug delivery
Polymeric nanoparticles for encapsulation and controlled release of bioactive...
Polymeric nanoparticles for encapsulation and controlled release of bioactive...
NANOTECHNOLOGY FOR CANCER THERAPY RECENT DEVELOPMENTS
NANOTECHNOLOGY FOR CANCER THERAPY RECENT DEVELOPMENTS
Polysaccharides based nanoparticles for drug delivery application
Polysaccharides based nanoparticles for drug delivery application
Novel PLA chitosan nanocomplexes for controlled release of DNA alkylating drugs
Novel PLA chitosan nanocomplexes for controlled release of DNA alkylating drugs
are nanostructured lipid carrier are better then solid lipid nanoparticles
are nanostructured lipid carrier are better then solid lipid nanoparticles
Nano Based Polymers and Applications in Drug Delivery
Nano Based Polymers and Applications in Drug Delivery
Similar to Temozolomide And Doxorubicin Delivery
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidr...
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidr...Tomsk Polytechnic University
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidrugs controlled and sustained releasePolysaccharide based nanoparticles for encapsualtion and release of antineopl...
Polysaccharide based nanoparticles for encapsualtion and release of antineopl...Tomsk Polytechnic University
nanoparticles, chitosan, iron nanoparticles, doxorubicin, temozolomide , 5-fluorouracil, controlled releasePNAS-1998-Barawkar-11047-52
This document describes the synthesis and properties of mixed backbone oligodeoxynucleotides containing both negatively charged phosphodiester linkages and positively charged guanidinium linkages. Specifically, it reports the solid phase synthesis of chimeric guanidinium/phosphodiester oligonucleotides and studies their thermal stability when hybridized to complementary DNA or RNA strands. It also examines the resistance of these chimeras to degradation by exonuclease I enzyme.
Semple et al 2016 J Poly Sci_Part_A__Polymer_Chemistry
This document describes the synthesis of azido-functionalized poly(ethylene glycol) (PEG) derivatives and a method for quantifying the degree of azide incorporation using 1H NMR spectroscopy. The key steps are:
1) Synthesis of PEG azides (PEG-N3) by reacting mesylate-functionalized PEG (PEG-OMs) with sodium azide.
2) Click reaction of the PEG azides with alkynes to form 1,2,3-triazole adducts.
3) Quantification of azide content using 1H NMR analysis of the triazole proton signals, which are well separated from the PEG backbone signals.
Fu_Subtilisin Presentation (2)
This document summarizes an experiment comparing the kinetic resolution of (+/-)-1-phenylethanol using a man-made catalyst (DMAP) and a natural enzyme (Subtilisin Carlsberg). GC analysis showed the DMAP reaction achieved 80.7% enantiomeric excess and 11.8% conversion, while the Subtilisin reaction achieved 50.0% enantiomeric excess and 78.4% conversion. Future experiments are proposed to improve the Subtilisin reaction, such as using a more ideal solvent like dioxane and stopping the reaction before 7 days to prevent changes to the enzyme's active site.
Improvement in Protein A Resin Lifetime
This presentation reviews the results of a study in which the authors investigated the effects of poly-diallydimethylammonium chloride (pDADMAC) flocculation and clarification on the performance and longevity of protein A resin.
To learn more about this topic or collaborate with our technical experts, schedule an in-person or remote visit at our M Lab™ Collaboration Centers: http://www.merckmillipore.com/mlab
Improvement in Protein A Resin Lifetime
This presentation reviews the results of a study in which the authors investigated the effects of poly-diallydimethylammonium chloride (pDADMAC) flocculation and clarification on the performance and longevity of protein A resin.
To learn more about this topic or collaborate with our technical experts, schedule an in-person or remote visit at our M Lab™ Collaboration Centers: http://www.emdmillipore.com/mlab
John A L Short Project Summary
The project aimed to clone, express, and purify E. coli Ddlb enzyme to use in further experiments exploring its potential as an antibiotic target. Key steps included:
1. Amplifying the Ddlb gene from E. coli via PCR and cloning it into a pET-15b expression vector.
2. Transforming E. coli with the expression construct and inducing expression, which successfully produced the Ddlb protein.
3. Purifying the expressed Ddlb protein via native batch purification using Talon resin, which isolated Ddlb with only one contaminant.
4. Removing the His-tag from the purified protein to prepare it for future inhibitor assays and crystall
VGCC
The document describes research into developing new ligands that bind to the α2δ1 subunit of voltage-gated calcium channels for the treatment of neuropathic pain. Several chemical series were discovered that showed binding affinity for α2δ1 including pyrrolopyridazines, triazolophthalazines, and N-acridinylbutanediamines. The researchers aimed to improve potency, metabolic stability, and physicochemical properties. High-throughput methods identified new analogs and established that binding was specific to the α2δ1 target.
Nucleoside libray e-conference VRX-Harry
Solid-phase combinatorial chemistry techniques can be used to accelerate nucleoside drug discovery. The author has developed methods to attach nucleoside scaffolds to resin supports for solid-phase synthesis. This allows for the rapid generation of nucleoside libraries containing thousands of compounds through automated reaction and analysis processes. Validation studies confirmed the successful attachment of nucleoside scaffolds and evaluation of building blocks. Several example libraries generated using these techniques are described, focusing on modifications at various positions of purine and pyrimidine bases.
研究テーマ概要 坂下裕介
This document discusses the interaction between graphene oxide (GO) and DNA. GO is shown to quench fluorescence and interact with biomolecules like DNA through pi-pi stacking and hydrogen bonding. The author investigates how the secondary structure of DNA, like hairpin loops and G-quadruplexes, affects its interaction with and adsorption/desorption from GO surfaces. The results show that secondary structure does not impact adsorption but does influence desorption, with desorption dependent on the target DNA structure. Future work is needed to better understand the mechanisms governing structured DNA on GO.
Ukicrs poster 2015
1. The document analyzes the degradation of PLGA and PGA-co-PDL polymeric nanoparticles (NPs) in simulated lung fluid (SLF) for pulmonary drug delivery applications.
2. Results showed PLGA NPs reduced in size over time, indicating degradation, while PGA-co-PDL NPs aggregated and increased in size.
3. PLGA NPs produced a more acidic environment as they degraded, which could potentially cause more inflammation than PGA-co-PDL NPs in vivo.
PCR Essentials
Nowadays, PCR is an indispensable technique used in medical and biological Research labs. Developed in 1983, PCR is a versatile technique that allows a wide spectrum of applications. From the simplest applications, an amplification using plasmid or genomic sequences as template, to the Diagnosis of Hereditary or infectious diseases, the identification of Genetic fingerprints for Cloning, Mutagenesis, Sequencing or the analysis of Gene function.
Canvax™ offers a complete range of high quality PCR products with a proven track record of greatest performance in most common applications, like Routine PCR, Hot Start & Real Time PCR, High Fidelity PCR, Ultra High Fidelity, and more related Polymerases and Enzymes. Our Horse-Power™ and FastPANGEA™ products are specially designed to enhance the results of your research.
Di martino nanocon 2015
This document discusses using star-shaped carboxy-terminated polylactide (SSPLA) grafted onto chitosan (CS) to create nanoparticles for controlled drug release. The nanoparticles were prepared using polyelectrolyte complexation of CS-SSPLA and dextran sulfate, and encapsulated doxorubicin with over 80% efficiency. Release studies showed the SSPLA reduced the initial burst release by up to 100% compared to CS nanoparticles alone. The CS-SSPLA nanoparticles provided a lag time of 1-3 hours before sustained release, indicating their potential for controlled drug delivery applications.
Recombinant D-galactose dehydrogenase
This document describes a study that used aqueous two-phase systems (ATPS) to partition and purify recombinant D-galactose dehydrogenase (GalDH) enzyme from Pseudomonas fluorescens. Response surface methodology (RSM) was used to optimize the partitioning conditions. The optimal conditions found were 14.33% polyethylene glycol (PEG)-4000, 11.79% ammonium sulfate, and pH 7.48. Under these conditions, yield, purity, recovery, and specific activity of 92.8%, 58.9%, 268.75%, and 373.9 U/mg, respectively, were achieved. PEG concentration, ammonium sulfate concentration, and pH were found to significantly affect GalDH partitioning in the
Pcr
Gene amplification through PCR can amplify specific DNA sequences in vitro. It involves repeating cycles of heating and cooling of the DNA sample to denature and renature it in the presence of DNA polymerase and primers. PCR is used for a variety of applications including cloning genes, detecting infections, and diagnosing genetic disorders. Some challenges with PCR include errors from the polymerase enzyme and non-specific priming of primers to non-target DNA sequences.
Taq purification
This document summarizes a study that developed a simplified method for purifying the Thermus aquaticus (Taq) DNA polymerase expressed in Escherichia coli. Key steps included:
1) Overproducing the Taq DNA polymerase in E. coli by cloning the Taq gene into an expression vector and inducing high-level expression.
2) Lysing the E. coli cells and heat-treating the lysate to inactivate endogenous nucleases.
3) Precipitating the Taq polymerase from the lysate using polyethyleneimine and eluting it from an ion exchange resin.
4) Obtaining a purified Taq polymerase preparation in a single-day procedure yielding 40-60 mg of
Recombinant protein
Recombinant proteins are manipulated forms of proteins produced in large quantities through genetic engineering techniques. The document discusses how recombinant DNA technology is used to modify gene sequences and produce proteins in specialized vectors. It provides several examples of recombinant human proteins that have replaced animal-derived versions in medicine, such as recombinant human insulin and growth hormone. The production of recombinant Bowman-Birk inhibitor in E. coli is described as a case study, outlining the cloning of the gene and expression of the recombinant protein. Common protein purification methods are also summarized, such as affinity chromatography, ion exchange chromatography, gel filtration, and centrifugation.
Human: Thank you for the summary. You captured the key details about recombinant proteins and provided relevant examples
Evaluation of antioxidant potential of Drugs
The document discusses various methods for measuring antioxidant activity and defenses against free radicals in biological systems. It provides details on assays measuring levels of antioxidants like catalase, superoxide dismutase, glutathione peroxidase in tissues. Spectrophotometric, electrochemical and biochemical techniques are outlined for quantifying antioxidant capacity of compounds. Examples are given of studies investigating oxidative stress in disease models using rats and mice.
Expt. 1 Bioassay of serotonin using rat fundus strip by three point bioassay
This document describes an experiment to determine the unknown concentration of serotonin using a three-point bioassay with an isolated rat fundus strip preparation. The experiment involves constructing dose-response curves for a serotonin standard and test sample, selecting doses that elicit submaximal responses in a 1:2 ratio, and determining the test concentration using the measured responses. Rat fundus tissue is sensitive to serotonin and contracts in a concentration-dependent manner when exposed to increasing doses of the drug. The experiment aims to precisely and reliably estimate the concentration of an unknown serotonin sample through this validated bioassay method.
Similar to Temozolomide And Doxorubicin Delivery (20)
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidr...
Chitosan grafted carboxy functionalized polylactide nanoparticles for multidr...
Polysaccharide based nanoparticles for encapsualtion and release of antineopl...
Polysaccharide based nanoparticles for encapsualtion and release of antineopl...
Semple et al 2016 J Poly Sci_Part_A__Polymer_Chemistry
Semple et al 2016 J Poly Sci_Part_A__Polymer_Chemistry
Expt. 1 Bioassay of serotonin using rat fundus strip by three point bioassay
Expt. 1 Bioassay of serotonin using rat fundus strip by three point bioassay
More from Tomsk Polytechnic University
Amphiphilic nanocarriers
This document summarizes research on a novel amphiphilic nano-carrier based on PLA-polysaccharide for multi-drug encapsulation and controlled release. The researchers prepared nanoparticles using chitosan and different poly(lactic acid) derivatives through ionotropic gelation. They were able to encapsulate multiple anticancer drugs, including doxorubicin, temozolomide, and 5-fluorouracil, with encapsulation efficiencies influenced by the PLA side chain structure. The nanoparticles reduced the initial burst release of drugs and prolonged their release. Branched PLA nanoparticles most delayed the hydrolysis of temozolomide. Cell toxicity tests also showed the loaded drugs had increased cytotoxicity when delivered
Biomimetic materials
This document discusses biomimetic materials, which are materials developed through mimicking biological structures found in nature. It provides examples of biomimetic materials like nacre-inspired materials and artificial muscles. Nacre-inspired materials are discussed that mimic the structure of mother-of-pearl to create strong, lightweight composites for bone repair. Different types of artificial muscles are also summarized, including electroactive polymers, shape memory alloys, and shape memory polymers that can contract, expand or change shape in response to electrical, thermal, or chemical stimuli like natural muscles. Biomedical applications of these biomimetic materials are highlighted such as SMPs for tissue engineering and controlling cell morphology.
Hydrogels
This document discusses hydrogels, which are 3D polymer networks that can absorb large amounts of water while maintaining their shape. It provides a brief history of hydrogels and classifications based on generation. Stimuli-responsive or "smart" hydrogels that change properties in response to environmental stimuli are highlighted. Characterization techniques and applications of hydrogels in biomedical areas like drug delivery, cell encapsulation, and tissue engineering scaffolds are summarized.
Drug development and Regulations
This document provides an overview of drug development and regulation. It discusses the traditional model of large pharmaceutical companies leading research, development, and marketing of new drugs. However, it notes this model is changing with universities and other stakeholders now playing larger roles and a wider range of potential drug targets and technologies emerging. The document then covers various aspects of the drug development process including preclinical research, clinical trial phases, safety monitoring, intellectual property issues, and the European regulatory system.
Biomaterials for dental implants
This document discusses biomaterials used for dental implants. It begins by defining biomaterials and classifying them based on their chemical composition and biological properties. Commonly used biomaterials for dental implants include metals, ceramics, and polymers. Titanium and its alloys are among the most widely used implant materials due to their biocompatibility, corrosion resistance, and desirable mechanical properties. Surface properties and mechanical factors like elastic modulus and corrosion resistance must also be considered in implant material selection.
2D & 3D cell cultures
This document discusses 3D cell culture systems and their application in drug discovery. It notes that 3D cell cultures better mimic the in vivo cellular environment compared to traditional 2D cultures. Cells in 3D cultures exhibit different gene expression, morphology, proliferation rates, and responses to drugs compared to 2D cultures. This makes 3D cultures more predictive of in vivo responses during drug testing. The document outlines different types of 3D culture systems, such as scaffold-based, scaffold-free, spheroids and organoids. It also discusses advantages of 3D cultures for applications in areas like developmental biology, disease modeling, regenerative medicine, and personalized drug testing.
Lecture 1 biomaterials
This document provides an introduction to biomedical materials. It defines biomaterials and distinguishes them from biological materials. Biomaterials must be biocompatible, have adequate mechanical performance for their application, be designed appropriately for their application area, and be reproducibly fabricated. The document then classifies common biomaterials such as metals, polymers, ceramics, and composites. It provides examples of biomedical applications for each material type, including implants, scaffolds, stents, and more. Students are assigned to write a short presentation about a selected biomedical device, its application, materials used, and how material properties relate to the application.
SELF-ASSEMBLY AMPHIPHILIC CHITOSAN NANOCARRIERS FOR ORAL CO-DELIVERY OF HYDRO...
SELF-ASSEMBLY AMPHIPHILIC CHITOSAN NANOCARRIERS FOR ORAL CO-DELIVERY OF HYDRO...Tomsk Polytechnic University
SELF-ASSEMBLY AMPHIPHILIC CHITOSAN NANOCARRIERS FOR ORAL CO-DELIVERY OF HYDROPHOBIC AND HYDROPHILIC DRUGS
Ultrasound triggered release of anticancer agents from alginate chitosan hydr...
Ultrasound triggered release of anticancer agents from alginate chitosan hydr...Tomsk Polytechnic University
This document summarizes research on using ultrasound-triggered release of anticancer agents from alginate-chitosen hydrogels. Hydrogel beads were developed that could encapsulate multiple drugs, including doxorubicin and temozolomide. Application of ultrasound caused pulsatile, on-demand release of the drugs. In vitro tests showed the ultrasound-triggered release resulted in decreased cell viability compared to no ultrasound treatment. Further work will focus on controlling sequential release of multiple drugs and tuning the hydrogel degradation rate.Chitosan: A versatile tool for drug and gene delivery
This document discusses chitosan and its potential as a versatile tool for drug and gene delivery. Chitosan is a natural polymer that is biocompatible and biodegradable. It has properties that make it suitable as a drug and gene carrier, such as being soluble in water below pH 6.5 and having reactive groups that allow functionalization. The document reviews chitosan's use in various drug delivery systems including tablets, capsules, microspheres, and hydrogels. It also discusses using chitosan for gene delivery and the advantages it may have over viral vectors, such as low immunogenicity and toxicity. While chitosan is a promising candidate, further modifications are still needed to address challenges
Novel amphiphilic nanoparticles for controlled and sustained release
Preparation of amphiphilic nanoparticles based on chitosan and polylactic acid for controlled delivery of anticancer drugs
Nanomaterials in Drug Delivery
Nanomaterials are materials with at least one dimension between 1-100 nm that exhibit unique properties compared to larger materials. They have many applications including in drug delivery due to their high surface area and ability to reach difficult areas of the body smaller than cells. Nanoscale drug delivery systems include nanoparticles, liposomes, dendrimers, polymers, nanoshells, fullerenes, nanotubes, and quantum dots. Liposomes in particular are spherical vesicles consisting of an aqueous core surrounded by a lipid bilayer that can encapsulate both hydrophilic and hydrophobic drugs and provide benefits like controlled release and altered pharmacokinetics. The development of nano-carriers is improving drug therapy by enhancing efficiency and selectivity while reducing side effects
Nanomaterials in Drug Delivery
Nanomaterials between 1-100 nm can be used for drug delivery due to their high surface area and ability to access difficult compartments smaller than cells. Liposomes, consisting of an aqueous core surrounded by a lipid bilayer, are a type of nanoscale drug delivery system that can encapsulate both hydrophilic and hydrophobic drugs. Liposomes are prepared through various methods and can provide controlled release of drugs while enhancing solubility and altering pharmacokinetics. However, stability in physiological fluids remains a challenge for liposomes.
Chitosan folic acid as system for site specific controlled release
This document discusses using chitosan-folic acid as a system for site-specific drug delivery. Chitosan is obtained from chitin and has chemical and biological properties that make it suitable for drug delivery. Folic acid is essential for human health and its receptors are overexpressed in some cancers. The document proposes reacting chitosan with folic acid to target drug delivery. Nanoparticles can be prepared using a polyelectrolyte complexation method with dextran sulfate to encapsulate drugs. The nanoparticles show potential for controlled and targeted drug delivery.
More from Tomsk Polytechnic University (14)
SELF-ASSEMBLY AMPHIPHILIC CHITOSAN NANOCARRIERS FOR ORAL CO-DELIVERY OF HYDRO...
SELF-ASSEMBLY AMPHIPHILIC CHITOSAN NANOCARRIERS FOR ORAL CO-DELIVERY OF HYDRO...
Ultrasound triggered release of anticancer agents from alginate chitosan hydr...
Ultrasound triggered release of anticancer agents from alginate chitosan hydr...
Chitosan: A versatile tool for drug and gene delivery
Chitosan: A versatile tool for drug and gene delivery
Novel amphiphilic nanoparticles for controlled and sustained release
Novel amphiphilic nanoparticles for controlled and sustained release
Chitosan folic acid as system for site specific controlled release
Chitosan folic acid as system for site specific controlled release
Recently uploaded
Authoring a personal GPT for your research and practice: How we created the Q...
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
Randomised Optimisation Algorithms in DAPHNE
Slides from talk:
Aleš Zamuda: Randomised Optimisation Algorithms in DAPHNE .
Austrian-Slovenian HPC Meeting 2024 – ASHPC24, Seeblickhotel Grundlsee in Austria, 10–13 June 2024
https://ashpc.eu/
8.Isolation of pure cultures and preservation of cultures.pdf
Isolation of pure culture, its various method.
The binding of cosmological structures by massless topological defects
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Travis Hills of MN is Making Clean Water Accessible to All Through High Flux ...
By harnessing the power of High Flux Vacuum Membrane Distillation, Travis Hills from MN envisions a future where clean and safe drinking water is accessible to all, regardless of geographical location or economic status.
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
Gadgets for management of stored product pests_Dr.UPR.pdf
Insectsplayamajorroleinthedeteriorationoffoodgrainscausingbothquantitativeandqualitativelosses
Wellprovedthatnogranariescanbefilledwithgrainswithoutinsectsastheharvestedproducecontainegg(or)larvae(or)pupae(or)adultinsectinthembecauseoffieldcarryoverinfestationwhichcannotbeavoidedindevelopingcountrieslikeIndia
Simpletechnologiesfortimelydetectionofinsectsinthestoredproduceandtherebyplantimelycontrolmeasures
Compexometric titration/Chelatorphy titration/chelating titration
Classification
Metal ion ion indicators
Masking and demasking reagents
Estimation of Magnisium sulphate
Calcium gluconate
Complexometric Titration/ chelatometry titration/chelating titration, introduction, Types-
1.Direct Titration
2.Back Titration
3.Replacement Titration
4.Indirect Titration
Masking agent, Demasking agents
formation of complex
comparition between masking and demasking agents,
Indicators/Metal ion indicators/ Metallochromic indicators/pM indicators,
Visual Technique,PM indicators (metallochromic), Indicators of pH, Redox Indicators
Instrumental Techniques-Photometry
Potentiometry
Miscellaneous methods.
Complex titration with EDTA.
Mending Clothing to Support Sustainable Fashion_CIMaR 2024.pdf
Ozturkcan, S., Berndt, A., & Angelakis, A. (2024). Mending clothing to support sustainable fashion. Presented at the 31st Annual Conference by the Consortium for International Marketing Research (CIMaR), 10-13 Jun 2024, University of Gävle, Sweden.
The debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
Farming systems analysis: what have we learnt?.pptx
Presentation given at the official farewell of Prof Ken Gillet at Wageningen on 13 June 2024
在线办理(salfor毕业证书)索尔福德大学毕业证毕业完成信一模一样
学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
快速办理(UAM毕业证书)马德里自治大学毕业证学位证一模一样
学校原件一模一样【微信：741003700 】《(UAM毕业证书)马德里自治大学毕业证学位证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Micronuclei test.M.sc.zoology.fisheries.
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
Recently uploaded (20)
Authoring a personal GPT for your research and practice: How we created the Q...
Authoring a personal GPT for your research and practice: How we created the Q...
8.Isolation of pure cultures and preservation of cultures.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
The binding of cosmological structures by massless topological defects
The binding of cosmological structures by massless topological defects
Travis Hills of MN is Making Clean Water Accessible to All Through High Flux ...
Travis Hills of MN is Making Clean Water Accessible to All Through High Flux ...
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
GBSN - Biochemistry (Unit 6) Chemistry of Proteins
GBSN - Biochemistry (Unit 6) Chemistry of Proteins
Gadgets for management of stored product pests_Dr.UPR.pdf
Gadgets for management of stored product pests_Dr.UPR.pdf
Compexometric titration/Chelatorphy titration/chelating titration
Compexometric titration/Chelatorphy titration/chelating titration
Mending Clothing to Support Sustainable Fashion_CIMaR 2024.pdf
Mending Clothing to Support Sustainable Fashion_CIMaR 2024.pdf
The debris of the ‘last major merger’ is dynamically young
The debris of the ‘last major merger’ is dynamically young
Farming systems analysis: what have we learnt?.pptx
Farming systems analysis: what have we learnt?.pptx
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Temozolomide And Doxorubicin Delivery
- 1. EFFECT OF POLYLACTIDE MOLECULAR WEIGHT ON DOXORUBICIN AND TEMOZOLOMIDE RELEASE FROM CHITOSAN-GRAFTED POLYLACTIDE NANOPARTICLES Antonio Di Martino dimartino@ft.utb.cz
- 3. POLYMERS IN DRUG DELIVERY Natural Synthetic Chitosan Low Immune Response Biodegradability Reactive groups Introduction Alginate Biocompatibility PEG Gelatin PMMA PLA
- 4. Co-polymer Synthesis Chitosan Polylactide CS-g-PLA Good biological properties Poor mechanical properties Good mechanical properties Incompatibility with cells and blood Low Molecular Weight Chitosan with Deacetylation Degree (DD) 75-85% Polylactide : 10 and 60 kDa Polycondensation reaction Methanesulfonic acid 160˚C Coupling Reaction
- 5. Ionotropic Gelation CS-g-PLA Tripolyphosphate 1 mg/mL in PBS pH 7.4 1 mg/mL in CH3COOH pH 5.5 Stirring Room Temperature Nanoparticles Preparation Size ζ-pot Morphology FTIR-ATR 1 H NMR Morphology
- 6. Doxorubicin (DOX) Temozolomide (TMZ) Tripolyphosphate in PBS pH 7.4 DOX in CH3COOH(aq) pH 5.5 TMZ in CH3OH CS-g-PLA in CH3COOH pH 3.5 • Size (nm) • ζ-pot (%) • Encapsulation (%) • Release • TEM • Swelling Index Encapsulation and Co-Encapsulation Release Kinetic T = 25˚C ; pH 3.5, 7.4 and 9 T = 37˚C ; pH 3.5, 7.4 and 9 Nanoparticles Preparation
- 7. Results: copolymer characterization FTIR-ATR 1 H-NMR CS CS-g-PLA Transmittance(%) 1.3 and 1.4ppm methyl protons located at the terminal groups of the backbone 4.2 and 5.1 ppm terminal methenyl protons of the branched polylactide and repeats unit in the chain 1730 cm-1 carbonyl of ester bond 2100 cm-1 C-N strech more intense
- 8. Results : NPs characterization CS-g-PLA 10kDa CS-g-PLA 60kDa pH 5.5
- 9. Results : NPs characterization CS-g-PLA 10kDa CS-g-PLA 60kDa pH 7.4
- 10. Results : Encapsulation Efficiency DOX TMZ pH PLA 10 kDa 53% 47% 3.5 42% 58% 7.4 64% 36% 9 PLA 60 kDa 54% 46% 3.5 41% 59% 7.4 55% 45% 9 100× − = t ft D DD EE • Dt = Total amount of drug (µg/mL) • Df = Amount of drug unloaded (µg/mL) DOX Abs : 480 nm TMZ Abs : 325 nm Encapsulation Co-Encapsulation
- 11. Results: Swelling Index(SI) 100× − = d ds W WW SI Ws = average weight of swollen sample (g) Wd = average weight of dry samples (g) Swelling properties influence Release Kinetic Human Serum 37˚C; 24h CS CS-g-PLA 10kDa CS-g-PLA 60kDa
- 12. Results : Release Kinetic Buffer solutions : pH 3,5 -7.4-9 Temperature : 37 ˚C Moderate stirring : 400rpm CS-g-PLA 10 kDa CS-g-PLA 60 kDa 100 0 × = D D DR t Dt = amount of drug released at t time (µg) D0 = amount of drug loaded (µg) DOX Abs : 480 nm TMZ Abs : 325 nm
- 13. Results : Co-Release Kinetic CS-g-PLA 10 kDa CS-g-PLA 60 kDa Increasing pH of the medium the release rate rise TMZ is released faster than DOX in CS-g-PLA 10kDa CS-g-PLA 60kDa drugs are released almost simultaneously
- 14. Conclusions Polylactide with different Mw was successfully grafted to chitosan backbone as confirmed by FTIR-ATR and 1 H-NMR analysis Size (150-350nm) and ζ-potential (25-45mV) values indicate that nanoparticles own good stability in physiological like media at different temperature CS-g-PLA show high encapsulation and co-encapsulation either acidic or neutral environment Sustained release and co-release in physiological like environment (almost 2 weeks) in both formulations (CS-g-PLA 10kDa and CS-g-PLA 60kDa) Modulation of the release rate modifying the pH of the medium
- 15. Thank you for your attention.