The document lists various EC numbers and their corresponding enzymatic reactions. It describes enzymes that form carbon-oxygen bonds (EC 6.1), carbon-sulfur bonds (EC 6.2), and carbon-nitrogen bonds (EC 6.3). Specifically, it provides details on the ligase enzymes that attach amino acids to tRNA molecules (EC 6.1.1), ligase enzymes that attach acids to coenzyme A (EC 6.2.1), and ligase enzymes that attach acids to ammonia or amines to form amides (EC 6.3.1). Over 30 specific enzyme reactions are detailed.

2. TATA NAMA ENZIM
EC EC 6.1.1.1 - EC 6.5.1.4
A. EC 6.1 Membentuk Ikatan Karbon-Oksigen
a. EC 6.1.1 Ligase Membentuk aminoasil-RNAt dan Senyawa Terkait
• EC 6.1.1.1 ligase tyrosine-RNAt
o ATP + L-tyrosine + tRNATyr
= AMP + diphosphate + L-tyrosyl-tRNATyr
• EC 6.1.1.2 ligase tryptophan-RNAt
o ATP + L-tryptophan + tRNATrp
= AMP + diphosphate + L-tryptophyl-tRNATrp
• EC 6.1.1.3 ligase treonin-RNAt
o ATP + L-threonine + tRNAThr
= AMP + diphosphate + L-threonyl-tRNAThr
• EC 6.1.1.4 ligase leusin-RNAt
o ATP + L-leucine + tRNALeu
= AMP + diphosphate + L-leucyl-tRNALeu
• EC 6.1.1.5 ligase isoleusin-RNAt
o ATP + L-isoleucine + tRNAIle
= AMP + diphosphate + L-isoleucyl-tRNAIle
• EC 6.1.1.6 ligase lisin-RNAt
o ATP + L-lysine + tRNALys
= AMP + diphosphate + L-lysyl-tRNALys
• EC 6.1.1.7 alanin ligase-RNAt
o ATP + L-alanine + tRNAAla
= AMP + diphosphate + L-alanyl-tRNAAla
• EC 6.1.1.8 dihapus
• EC 6.1.1.9 ligase valin-RNAt
o ATP + L-valine + tRNAVal
= AMP + diphosphate + L-valyl-tRNAVal
• EC 6.1.1.10 ligase metionin-RNAt
o ATP + L-methionine + tRNAMet
= AMP + diphosphate + L-methionyl-tRNAMet
• EC 6.1.1.11 ligase serin-RNAt
o ATP + L-serine + tRNASer
= AMP + diphosphate + L-seryl-tRNASer
• EC 6.1.1.12 ligase aspartat-RNAt
o ATP + L-aspartate + tRNAAsp
= AMP + diphosphate + L-aspartyl-tRNAAsp
• EC 6.1.1.13 ligase D-alanin-poli (phosphoribitol)
o ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-
poly(ribitol phosphate)
• EC 6.1.1.14 ligase glisin-RNAt
o ATP + glycine + tRNAGly
= AMP + diphosphate + glycyl-tRNAGly
• EC 6.1.1.15 ligase prolin-RNAt
o TP + L-proline + tRNAPro
= AMP + diphosphate + L-prolyl-tRNAPro
• EC 6.1.1.16 ligase sistein-RNAt
o ATP + L-cysteine + tRNACys
= AMP + diphosphate + L-cysteinyl-tRNACys
• EC 6.1.1.17 ligase glutamat-RNAt
o ATP + L-glutamate + tRNAGlu
= AMP + diphosphate + L-glutamyl-tRNAGlu
• EC 6.1.1.18 ligase glutamin-RNAt
o ATP + L-glutamine + tRNAGln
= AMP + diphosphate + L-glutaminyl-tRNAGln
• EC 6.1.1.19 ligase arginin-RNAt
o ATP + L-arginine + tRNAArg
= AMP + diphosphate + L-arginyl-tRNAArg
• EC 6.1.1.20 ligase fenilalanin-RNAt
o ATP + L-phenylalanine + tRNAPhe
= AMP + diphosphate + L-phenylalanyl-tRNAPhe
• EC 6.1.1.21 ligase histidin-RNAt
o ATP + L-histidine + tRNAHis
= AMP + diphosphate + L-histidyl-tRNAHis
• EC 6.1.1.22 ligase asparagin-RNAt
o ATP + L-asparagine + tRNAAsn
= AMP + diphosphate + L-asparaginyl-tRNAAsn
• EC 6.1.1.23 ligase aspartat-RNAtAsn

3. o ATP + L-aspartate + tRNAAsx
= AMP + diphosphate + aspartyl-tRNAAsx
• EC 6.1.1.24 ligase glutamat-RNAtGln
o ATP + L-glutamate + tRNAGlx
= AMP + diphosphate + glutamyl-tRNAGlx
• EC 6.1.1.25 ligase lisin-RNAtPyl
o ATP + L-lysine + tRNAPyl
= AMP + diphosphate + L-lysyl-tRNAPyl
• EC 6.1.1.26 ligase pyrrolysine-RNAtPyl
o ATP + L-pyrrolysine + tRNAPyl
= AMP + diphosphate + L-pyrrolysyl-tRNAPyl
• EC 6.1.1.27 ligase O-phosphoserine-RNAt
o ATP + O-phospho-L-serine + tRNACys
= AMP + diphosphate + O-phospho-L-seryl-
tRNACys
b. EC 6.1.2 Ligase Asam-Alkohol (synthases ester)
• EC 6.1.2.1 Ligase D-alanin-(R)-laktat
o D-alanine + (R)-lactate + ATP = D-alanyl-(R)-lactate + ADP + phosphate
B. EC 6.2 Pembentukan Ikatan karbon-Sulfur
a. EC 6.2.1 Ligases Asam-tiol
• EC 6.2.1.1 ligase asetat-CoA
o ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
• EC 6.2.1.2 ligase butirat-CoA
o ATP + an acid + CoA = AMP + diphosphate + an acyl-CoA
• EC 6.2.1.3 ligase rantai panjang asam lemak-CoA
o ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA
• EC 6.2.1.4 ligase suksinat-KoA (pembentuk GDP)
o GTP + succinate + CoA = GDP + phosphate + succinyl-CoA
• EC 6.2.1.5 ligase suksinat-KoA (pembentuk ADP)
o ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
• EC 6.2.1.6 ligase glutarat-CoA
o ATP + glutarate + CoA = ADP + phosphate + glutaryl-CoA
• EC 6.2.1.7 ligase kolat-CoA
o (1) ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA
o (2) ATP + (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oate + CoA = AMP +
diphosphate + (25R)-3α,7α,12α-trihydroxy-5β-cholestanoyl-CoA
• EC 6.2.1.8 ligase oksalat-CoA
o ATP + oxalate + CoA = AMP + diphosphate + oxalyl-CoA
• EC 6.2.1.9 ligase malat-CoA
o ATP + malate + CoA = ADP + phosphate + malyl-CoA
• EC 6.2.1.10 ligase asam-KoA (pembentuk GDP)
o GTP + an acid + CoA = GDP + phosphate + acyl-CoA
• EC 6.2.1.11 ligase biotin-CoA
o ATP + biotin + CoA = AMP + diphosphate + biotinyl-CoA
• EC 6.2.1.12 Ligase 4-coumarate-CoA
o ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA
• EC 6.2.1.13 ligase asetat-KoA (pembentuk ADP)
o ATP + acetate + CoA = ADP + phosphate + acetyl-CoA
• EC 6.2.1.14 Ligase 6-carboxyhexanoate-CoA
o ATP + 6-carboxyhexanoate + CoA = AMP + diphosphate + 6-carboxyhexanoyl-CoA
• EC 6.2.1.15 ligase arachidonate-CoA
o ATP + arachidonate + CoA = AMP + diphosphate + arachidonoyl-CoA

4. • EC 6.2.1.16 ligase acetoacetate-CoA
o ATP + acetoacetate + CoA = AMP + diphosphate + acetoacetyl-CoA
• EC 6.2.1.17 ligase propionat-CoA
o ATP + propanoate + CoA = AMP + diphosphate + propanoyl-CoA
• EC 6.2.1.18 ligase sitrat-CoA
o ATP + citrate + CoA = ADP + phosphate + (3S)-citryl-CoA
• EC 6.2.1.19 ligase rantai panjang asam lemak – komponen luciferin
o ATP + an acid + protein = AMP + diphosphate + an acyl-protein thioester
• EC 6.2.1.20 ligase rantai panjang asam lemak-[asil pembawa protein]
o ATP + an acid + an [acyl-carrier protein] = AMP + diphosphate + an acyl-[acyl-carrier
protein]
• EC 6.2.1.21 ditutupi oleh EC 6.2.1.30
• EC 6.2.1.22 ligase [sitrat (pro-3S)-lyase]
o ATP + acetate + [citrate (pro-3S)-lyase](thiol form) = AMP + diphosphate + [citrate
(pro-3S)-lyase](acetyl form)
• EC 6.2.1.23 ligase dicarboxylate-CoA
o ATP + an αω-dicarboxylic acid + CoA = AMP + diphosphate + an ω-carboxyacyl-CoA
• EC 6.2.1.24 ligase phytanate-CoA
o ATP + phytanate + CoA = AMP + diphosphate + phytanoyl-CoA
• EC 6.2.1.25 ligase benzoat-CoA
o ATP + benzoate + CoA = AMP + diphosphate + benzoyl-CoA
• EC 6.2.1.26 Ligase o-succinylbenzoate-CoA
o ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 4-(2-carboxyphenyl)-4-
oxobutanoyl-CoA
• EC 6.2.1.27 Ligase 4-hydroxybenzoate-CoA
o ATP + 4-hydroxybenzoate + CoA = AMP + diphosphate + 4-hydroxybenzoyl-CoA
• EC 6.2.1.28 ligase 3α, 7α-dihidroksi-5β-cholestanate-CoA
o ATP + (25R)-3α,7α-dihydroxy-5β-cholestan-26-oate + CoA = AMP + diphosphate +
(25R)-3α,7α-dihydroxy-5β-cholestanoyl-CoA
• EC 6.2.1.29 sekarang EC 6.2.1.7
• EC 6.2.1.30 ligase phenylacetate-CoA
o ATP + phenylacetate + CoA = AMP + diphosphate + phenylacetyl-CoA
• EC 6.2.1.31 Ligase 2-furoate-CoA
o ATP + 2-furoate + CoA = AMP + diphosphate + 2-furoyl-CoA
• EC 6.2.1.32 ligase anthranilate-CoA
o ATP + anthranilate + CoA = AMP + diphosphate + anthranilyl-CoA
• EC 6.2.1.33 Ligase 4-chlorobenzoate-CoA
o 4-chlorobenzoate + CoA + ATP = 4-chlorobenzoyl-CoA + AMP + diphosphate
• EC 6.2.1.34 sintesa trans-feruloyl-CoA
o ferulic acid + CoA + ATP = feruloyl-CoA + products of ATP breakdown
• EC 6.2.1.35 ACP-SH: ligase asetat
o ATP + acetate + an [acyl-carrier protein] = AMP + diphosphate + an acetyl-[acyl-
carrier protein]
• EC 6.2.1.36 Sintesa 3-hidroksipropionil – CoA
o 3-hydroxypropionate + ATP + coenzyme A = 3-hydroxypropionyl-CoA + AMP +
diphosphate
C. EC 6.3 Membentuk Ikatan Carbon-Nitrogen
a. EC 6.3.1 Ligase Asam-Amonia (atau Amin) (Synthases Amide)
• EC 6.3.1.1 ligase aspartat-amonia
o ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine

5. • EC 6.3.1.2 ligase glutamat-amonia
o ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
• EC 6.3.1.3 sekarang EC 6.3.4.13
• EC 6.3.1.4 ligase aspartat-amonia (pembentuk ADP)
o ATP + L-aspartate + NH3 = ADP + phosphate + L-asparagine
• EC 6.3.1.5 sintesa NAD+
o ATP + deamido-NAD+
+ NH3 = AMP + diphosphate + NAD+
• EC 6.3.1.6 ligase glutamat-etilamin
o ATP + L-glutamate + ethylamine = ADP + phosphate + N5
-ethyl-L-glutamine
• EC 6.3.1.7 ligase 4-methyleneglutamate-amonia
o ATP + 4-methylene-L-glutamate + NH3 = AMP + diphosphate + 4-methylene-L-
glutamine
• EC 6.3.1.8 sintesa glutathionylspermidine
o glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate
• EC 6.3.1.9 sintesa trypanothione
o glutathione + glutathionylspermidine + ATP = N1
,N8
-bis(glutathionyl)spermidine +
ADP + phosphate
• EC 6.3.1.10 sintesa adenosylcobinamide-fosfat
o 1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP +
phosphate + adenosylcobinamide phosphate
o (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate +
adenosylcobinamide
• EC 6.3.1.11 ligase glutamat-putresin
o ATP + L-glutamate + putrescine = ADP + phosphate + γ-L-glutamylputrescin
• EC 6.3.1.12 ligase D-aspartat)
o ATP + D-aspartate + [β-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-
Ala)]n = [β-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-6-N-(β-D-Asp)-L-Lys-D-Ala-
D-Ala)]n + ADP + phosphate
• EC 6.3.1.13 L-cysteine: ligase 2-amino-2-deoksi-α-D-glucopyranoside 1D-myo-inositol
o 1-O-(2-amino-2-deoxy-α-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-
O-[2-(L-cysteinamido)-2-deoxy-α-D-glucopyranosyl]-1D-myo-inositol + AMP +
diphosphate
• EC 6.3.1.14 ligase diphthine-amonia
o ATP + diphthine + NH3 = ADP + phosphate + diphthamide
b. EC 6.3.2 Ligases Asam-Asam Amino- (Synthases Peptida)
• EC 6.3.2.1 ligase pantoate-β-alanin
o ATP + (R)-pantoate + β-alanine = AMP + diphosphate + (R)-pantothenate
• EC 6.3.2.2 ligase glutamat-cysteine
o ATP + L-glutamate + L-cysteine = ADP + phosphate + γ-L-glutamyl-L-cysteine
• EC 6.3.2.3 glutathione sintesa
o ATP + γ-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione
• EC 6.3.2.4 Ligase D-alanin-D-alanin
o ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine
• EC 6.3.2.5 ligase phosphopantothenate-cysteine
o CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-[(R)-4'-
phosphopantothenoyl]-L-cysteine
• EC 6.3.2.6 sintesa phosphoribosylaminoimidazolesuccinocarboxamide

6. o ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP
+ phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-
carboxamido]succinate
• EC 6.3.2.7 ligase UDP-N- acetylmuramoyl-L-alanyl-D-glutamat-L-lisin
o ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine = ADP + phosphate +
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine
• EC 6.3.2.8 ligase UDP-N- acetylmuramate-L-alanin
o ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-
acetylmuramoyl-L-alanine
• EC 6.3.2.9 ligase UDP-N-acetylmuramoylalanin-D-glutamat
o ATP + UDP-N-acetylmuramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-
N-acetylmuramoyl-L-alanyl-D-glutamate
• EC 6.3.2.10 ligase UDP-N- acetylmuramoylalanyl-tripeptide-D-alanyl-D-alanin
o ATP + UDP-N-acetylmuramoyl-L-alanyl-γ-D-glutamyl-L-lysine + D-alanyl-D-alanine =
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanyl-
D-alanine
• EC 6.3.2.11 sintesa carnosine
o ATP + L-histidine + β-alanine = ADP + phosphate + carnosine
• EC 6.3.2.12 sintesa dihydrofolate
o ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-
dihydropteroylglutamate
• EC 6.3.2.13 ligase UDP-N-acetylmuramoyl-L-alanyl-D-glutamat-2 ,6-diaminopimelate
o ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-
diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-γ-
glutamyl-meso-2,6-diaminoheptanedioate
• EC 6.3.2.14 ligase 2,3-dihydroxybenzoate-serin
o ATP + 2,3-dihydroxybenzoate + L-serine = products of ATP breakdown + N-(2,3-
dihydroxybenzoyl)-L-serine
• EC 6.3.2.15 dihapus, karena EC 6.3.2.10
• EC 6.3.2.16 ligase D-alanin-alanyl-poli (glycerolphosphate)
o ATP + D-alanine + alanyl-poly(glycerolphosphate) = ADP + phosphate + D-alanyl-
alanyl-poly(glycerolphosphate)
• EC 6.3.2.17 sintesa tetrahydrofolate
o ATP + tetrahydropteroyl-[γ-Glu]n + L-glutamate = ADP + phosphate +
tetrahydropteroyl-[γ-Glu]n+1
• EC 6.3.2.18 sintesa γ-glutamylhistamine
o ATP + L-glutamate + histamine = products of ATP breakdown + Nα
-γ-L-
glutamylhistamine
• EC 6.3.2.19 ligase ubiquitin-protein
o ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysin
• EC 6.3.2.20 Sintetase indoleacetate-lisin
o ATP + (indol-3-yl)acetate + L-lysine = ADP + phosphate + N6
-[(indole-3-yl)acetyl]-L-
lysine
• EC 6.3.2.21 ligase ubiquitin-kalmodulin
o n ATP + calmodulin + n ubiquitin = n AMP + n diphosphate + (ubiquitin)n-calmodulin
• EC 6.3.2.22 sekarang EC 6.3.1.14
• EC 6.3.2.23 sintesa homoglutathione
o ATP + γ-L-glutamyl-L-cysteine + β-alanine = ADP + phosphate + γ-L-glutamyl-L-
cysteinyl-β-alanine
• EC 6.3.2.24 ligase tyrosine-arginine
o ATP + L-tyrosine + L-arginine = AMP + diphosphate + L-tyrosyl-L-arginine
• EC 6.3.2.25 ligase tubulin-tirosin

7. o ATP + detyrosinated α-tubulin + L-tyrosine = α-tubulin + ADP + phosphate
• EC 6.3.2.26 Sintesa N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valin
o 3 ATP + L-2-aminohexanedioate + L-cysteine + L-valine + H2O = 3 AMP + 3
diphosphate + N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine
• EC 6.3.2.27 sintesa aerobactin
o 4 ATP + citrate + 2 N6
-acetyl-N6
-hydroxy-L-lysine + 2 H2O = 4 ADP + 4 phosphate +
aerobactin
• EC 6.3.2.28 ligase L-amino-asam α-
o ATP + an L-amino acid + an L-amino acid = ADP + phosphate + L-aminoacyl-L-amino
acid
• EC 6.3.2.29 sintesa cyanophycin (menambah L-aspartat-)
o ATP + [L-Asp(4-L-Arg)]n + L-Asp = ADP + phosphate + [L-Asp(4-L-Arg)]n—L-Asp
• EC 6.3.2.30 sintesa cyanophycin (menambah L-arginine-)
o ATP + [L-Asp(4-L-Arg)]n—L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1
• EC 6.3.2.31 ligase koenzim F420-0: L-glutamat
o GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1
• EC 6.3.2.32 ligase koenzim γ-F420-2: α-L-glutamat
o ATP + coenzyme γ-F420-2 + L-glutamate = ADP + phosphate + coenzyme α-F420-3
• EC 6.3.2.33 sintesa tetrahydrosarcinapterin
o ATP + tetrahydromethanopterin + L-glutamate = ADP + phosphate + 5,6,7,8-
tetrahydrosarcinapterin
• EC 6.3.2.34 ligase koenzim F420-1: γ-L-glutamat
o GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme γ-F420-2
• EC 6.3.2.35 Ligase D-alanin-D-serin
o D-alanine + D-serine + ATP = D-alanyl-D-serine + ADP + phosphate
• EC 6.3.2.36 Ligase 4-phosphopantoate-β-alanin
o ATP + (R)-4-phosphopantoate + β-alanine = AMP + diphosphate + (R)-4'-
phosphopantothenate
c. EC 6.3.3 Ligases cyclo
• EC 6.3.3.1 ligase cyclo phosphoribosylformylglycinamidine
o ATP + 2-(formamido)-N1
-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-
amino-1-(5-phospho-D-ribosyl)imidazole
• EC 6.3.3.2 ligase cyclo 5-formyltetrahydrofolate
o ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate
• EC 6.3.3.3 sintesa dethiobiotin
o ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin
• EC 6.3.3.4 sintesa (carboxyethyl) β-laktam- arginin
o ATP + L-N2
-(2-carboxyethyl)arginine = AMP + diphosphate +
deoxyamidinoproclavaminate
d. EC 6.3.4 Ligases Karbon-Nitrogen Lain-lain
• EC 6.3.4.1 sintesa GMP
o ATP + xanthosine 5'-phosphate + NH3 = AMP + diphosphate + GMP
• EC 6.3.4.2 sintesa CTP
o ATP + UTP + NH3 = ADP + phosphate + CTP
• EC 6.3.4.3 ligase formate-tetrahydrofolate
o ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
• EC 6.3.4.4 sintesa adenylosuccinate
o GTP + IMP + L-aspartate = GDP + phosphate + N6
-(1,2-dicarboxyethyl)-AMP
• EC 6.3.4.5 sintesa argininosuccinate

8. o ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nω
-L-arginino)succinate
• EC 6.3.4.6 urea karboksilase
o ATP + urea + HCO3
-
= ADP + phosphate + urea-1-carboxylate
• EC 6.3.4.7 ligase ribosa-5-fosfat-amonia
o ATP + ribose 5-phosphate + NH3 = ADP + phosphate + 5-phosphoribosylamine
• EC 6.3.4.8 ligase imidazoleacetate-phosphoribosyldiphosphate
o ATP + imidazole-4-acetate + 5-phosphoribosyl diphosphate = ADP + phosphate + 1-
(5-phosphoribosyl)imidazole-4-acetate + diphosphate
• EC 6.3.4.9 ligase biotin-[methylmalonyl-CoA-carboxytransferase]
o ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxytransferase] = AMP +
diphosphate + [methylmalonyl-CoA:pyruvate carboxytransferase]
• EC 6.3.4.10 ligase biotin-[propionil-CoA-karboksilase (ATP-hydrolysing)]
o ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP +
diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
• EC 6.3.4.11 ligase biotin-[methylcrotonoyl-CoA-karboksilase]
o ATP + biotin + apo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] =
AMP + diphosphate + [3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)]
• EC 6.3.4.12 ligase glutamat-metilamin
o ATP + L-glutamate + methylamine = ADP + phosphate + N5
-methyl-L-glutamine
• EC 6.3.4.13 ligase phosphoribosylamine-glisin
o ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1
-(5-phospho-D-
ribosyl)glycinamide
• EC 6.3.4.14 biotin karboksilase
o ATP + biotin-carboxyl-carrier protein + CO2 = ADP + phosphate + carboxybiotin-
carboxyl-carrier protein
• EC 6.3.4.15 ligase biotin-[asetil-KoA-karboksilase]
o ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP +
diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
• EC 6.3.4.16 sintesa karbamoil-fosfat (amonia)
o 2 ATP + NH3 + CO2 + H2O = 2 ADP + phosphate + carbamoyl phosphate
• EC 6.3.4.17 ligase formate-dihydrofolate
o ATP + formate + dihydrofolate = ADP + phosphate + 10-formyldihydrofolate
• EC 6.3.4.18 sintesa 5 - (karboksiamino) imidazola ribonucleotide
o ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3
-
= ADP + phosphate + 5-
carboxyamino-1-(5-phospho-D-ribosyl)imidazole
e. EC 6.3.5 Ligases Carbon-Nitrogen dengan Glutamine sebagai amido-N-Donor
• EC 6.3.5.1 sintesa NAD+
(hidrolisis glutamin)
o ATP + deamido-NAD+
+ L-glutamine + H2O = AMP + diphosphate + NAD+
+ L-
glutamate
• EC 6.3.5.2 sintesa GMP (hidrolisis glutamin)
o ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP +
L-glutamate
• EC 6.3.5.3 sintesa phosphoribosylformylglycinamidine
o ATP + N2
-formyl-N1
-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP +
phosphate + 2-(formamido)-N1
-(5-phospho-D-ribosyl)acetamidine + L-glutamate
• EC 6.3.5.4 sintesa asparagin (hidrolisis glutamin)
o ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-
glutamate
(1a) L-glutamine + H2O = L-glutamate + NH3
(1b) ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine
• EC 6.3.5.5 sintesa karbamoil-fosfat (hidrolisis glutamin)

9. o 2 ATP + L-glutamine + HCO3
-
+ H2O = 2 ADP + phosphate + L-glutamate + carbamoyl
phosphate
(1) L-glutamine + H2O = L-glutamate + NH3
• EC 6.3.5.6 sintesa asparaginyl-RNAt (hidrolisis glutamin)
o ATP + aspartyl-tRNAAsn
+ L-glutamine = ADP + phosphate + asparaginyl-tRNAAsn
+ L-
glutamate
• EC 6.3.5.7 sintesa glutaminyl-RNAt (hidrolisis glutamin)
o ATP + glutamyl-tRNAGln
+ L-glutamine = ADP + phosphate + glutaminyl-tRNAGln
+ L-
glutamate
• EC 6.3.5.8 sekarang EC 2.6.1.85
• EC 6.3.5.9 sintesa hydrogenobyrinic asam a, c-diamide (hidrolisis glutamin)
o 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate +
hydrogenobyrinic acid a,c-diamide + 2 L-glutamate
• EC 6.3.5.10 sintesa asam adenosylcobyric (hidrolisis glutamin)
o 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4
phosphate + adenosylcobyric acid + 4 L-glutamate
• EC 6.3.5.11 sintasa cobyrinate a, c- diamide
o 2 ATP + cobyrinate + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + cobyrinate a,c-
diamide + 2 L-glutamate
(1a) ATP + cobyrinate + L-glutamine + H2O = ADP + phosphate + cobyrinate c-
monamide + L-glutamate
(1b) ATP + cobyrinate c-monamide + L-glutamine + H2O = ADP + phosphate +
cobyrinate a,c-diamide + L-glutamate
D. EC 6.4 Membentuk Ikatan Karbon - Karbon
• EC 6.4.1.1 piruvat karboksilase
o ATP + pyruvate + HCO3
-
= ADP + phosphate + oxaloacetate
• EC 6.4.1.2 asetil-KoA Karboksilase
o ATP + acetyl-CoA + HCO3
-
= ADP + phosphate + malonyl-CoA
• EC 6.4.1.3 propionil-CoA Karboksilase
o ATP + propanoyl-CoA + HCO3
-
= ADP + phosphate + (S)-methylmalonyl-CoA
• EC 6.4.1.4 methylcrotonoyl-CoA Karboksilase
o ATP + 3-methylcrotonoyl-CoA + HCO3
-
= ADP + phosphate + 3-methylglutaconyl-
CoA
• EC 6.4.1.5 geranoyl-CoA Karboksilase
o ATP + geranoyl-CoA + HCO3
-
= ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-
2-enedioyl-CoA
• EC 6.4.1.6 aseton karboksilase
o acetone + CO2 + ATP + 2 H2O = acetoacetate + AMP + 2 phosphate
• EC 6.4.1.7 2-oxoglutarate Karboksilase
o ATP + 2-oxoglutarate + HCO3
-
= ADP + phosphate + oxalosuccinate
E. EC 6.5 Membentuk Ikatan Ester fosfat
• EC 6.5.1.1 ligase DNA (ATP)
o ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m = AMP + diphosphate +
(deoxyribonucleotide)n+m
• EC 6.5.1.2 ligase DNA (NAD+
)
o NAD+
+ (deoxyribonucleotide)n + (deoxyribonucleotide)m = AMP + nicotinamide
nucleotide + (deoxyribonucleotide)n+m
• EC 6.5.1.3 ligase RNA (ATP)
o ATP + (ribonucleotide)n + (ribonucleotide)m = AMP + diphosphate +
(ribonucleotide)n+m

10. • EC 6.5.1.4 RNA-3'- Adenilat fosfat
o ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-
phosphate
F. EC 6.6 Membentuk Ikatan Nitrogen-Metal
a. EC 6.6.1 Koordinasi Pembentukan Kompleks
• EC 6.6.1.1 magnesium chelatase
o ATP + protoporphyrin IX + Mg2+
+ H2O = ADP + phosphate + Mg-protoporphyrin IX
+ 2 H+
• EC 6.6.1.2 cobaltochelatase
o ATP + hydrogenobyrinic acid a,c-diamide + Co2+
+ H2O = ADP + phosphate +
cob(II)yrinic acid a,c-diamide + H+